LAT2 Antibody (R31149)

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LAT2 Antibody (R31149) Catalog No. Formulation Size R31149 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal UniProt Q9GZY6 Applications Western blot : 0.5-1ug/ml Limitations This LAT2 antibody is available for research use only. Western blot testing of LAT2 antbody; Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng Description Linker for Activation of T Cells Family, Member 2, also known as LAB or NTAL , is a protein that in humans is encoded by the LAT2 gene. By genomic sequence analysis, Doyle et al.(2000) mapped the gene to chromosome 7q11.23. They noted that the flanking genes are highly conserved between mouse and human. By immunoprecipitation and immunoblot analysis, Brdicka et al.(2002) found that the protein associated with GRB2, SOS1, and GAB1 after B-cell receptor activation. Janssen et al.(2003) determined that phosphorylation of LAT2 is likely mediated by SYK upon B-cell receptor stimulation. Binding analysis indicated associations with GRB2, and, indirectly, with PLCG1 and PLCG2. Application Notes The stated application concentrations are suggested starting amounts. Titration of the LAT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen An amino acid sequence from the N-terminus of human Linker for Activation of T Cells Family, Member 2 (KRSEKIYQQRSLRED) was used as the immunogen for this LAT2 antibody. Storage After reconstitution, the LAT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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LAT2 Monoclonal Antibody, Clone NAP-07 (PE)
LAT2 monoclonal antibody, clone NAP-07 (PE) Catalog # : MAB4522 規格 : [ 100 ug ] List All Specification Application Image Product Mouse monoclonal antibody raised against partial recombinant LAT2. Western Blot Description: Immunocytochemistry Immunogen: Recombinant protein corresponding to partial human LAT2. Immunoprecipitation Flow Cytometry Host: Mouse Theoretical MW 25-30 (kDa): Reactivity: Human, Mouse Specificity: This antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25-30 KDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells. Form: Liquid Conjugation: PE Concentration: 0.1 mg/mL Isotype: IgG1 Recommend Flow Cytometry (1:30) Usage: The optimal working dilution should be determined by the end user. Storage Buffer: In PBS (0.2% BSA, 0.09% sodium azide) Storage Store in the dark at 4°C. Do not freeze. Instruction: Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing. Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Datasheet: Download Publication Reference 1. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. Lebduska P, Korb J, Tumova M, Heneberg P, Draber P.J Immunol Methods. 2007 Dec 1;328(1-2):139-51. Epub 2007 Sep 18. 2. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P.J Exp Med.
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