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Dysfunctional LAT2 Amino Acid Transporter Is Associated with Cataract in Mouse and Humans

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Dysfunctional LAT2 Amino Acid Transporter Is Associated with Cataract in Mouse and Humans Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2019 Dysfunctional LAT2 Amino Acid Transporter Is Associated With Cataract in Mouse and Humans Knöpfel-Boiadjieva, Emilia; Vilches, Clara; Camargo, Simone M R; Errasti-Murugarren, Ekaitz; Stäubli, Andrina; Mayayo, Clara; Munier, Francis L; Miroshnikova, Nataliya; Poncet, Nadège; Junza, Alexandra; Bhattacharya, Shomi S; Prat, Esther; Berry, Vanita; Berger, Wolfgang; Heon, Elise; Moore, Anthony T; Yanes, Óscar; Nunes, Virginia; Palacín, Manuel; Verrey, Francois; Kloeckener-Gruissem, Barbara Abstract: Cataract, the loss of ocular lens transparency, accounts for 50% of worldwide blindness and has been associated with water and solute transport dysfunction across lens cellular barriers. We show that neutral amino acid antiporter LAT2 ) and uniporter TAT1 () are expressed on mouse ciliary epithelium and LAT2 also in lens epithelium. Correspondingly, deletion of LAT2 induced a dramatic decrease in lens essential amino acid levels that was modulated by TAT1 defect. Interestingly, the absence of LAT2 led to increased incidence of cataract in mice, in particular in older females, and a synergistic effect was observed with simultaneous lack of TAT1. Screening in patients diagnosed with congenital or age-related cataract yielded one homozygous single nucleotide deletion segregating in a family with congenital cataract. Expressed in HeLa cells, this LAT2 mutation did not support amino acid uptake. Heterozygous LAT2 variants were also found in patients with cataract some of which showed a reduced transport function when expressed in HeLa cells. Whether heterozygous LAT2 variants may contribute to the pathology of cataract needs to be further investigated. Overall, our results suggest that defects of amino acid transporter LAT2 are implicated in cataract formation, a situation that may be aggravated by TAT1 defects. DOI: https://doi.org/10.3389/fphys.2019.00688 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-171531 Journal Article Published Version The following work is licensed under a Creative Commons: Attribution 4.0 International (CC BY 4.0) License. Originally published at: Knöpfel-Boiadjieva, Emilia; Vilches, Clara; Camargo, Simone M R; Errasti-Murugarren, Ekaitz; Stäubli, Andrina; Mayayo, Clara; Munier, Francis L; Miroshnikova, Nataliya; Poncet, Nadège; Junza, Alexandra; Bhattacharya, Shomi S; Prat, Esther; Berry, Vanita; Berger, Wolfgang; Heon, Elise; Moore, Anthony T; Yanes, Óscar; Nunes, Virginia; Palacín, Manuel; Verrey, Francois; Kloeckener-Gruissem, Barbara (2019). Dysfunctional LAT2 Amino Acid Transporter Is Associated With Cataract in Mouse and Humans. Frontiers in Physiology, 10:688. DOI: https://doi.org/10.3389/fphys.2019.00688 2 fphys-10-00688 June 3, 2019 Time: 19:57 # 1 ORIGINAL RESEARCH published: 04 June 2019 doi: 10.3389/fphys.2019.00688 Dysfunctional LAT2 Amino Acid Transporter Is Associated With Cataract in Mouse and Humans Emilia Boiadjieva Knöpfel1,2,3†, Clara Vilches4,5†, Simone M. R. Camargo1,2†, Ekaitz Errasti-Murugarren5,6†, Andrina Stäubli7,8, Clara Mayayo4,6, Francis L. Munier9, Edited by: Nataliya Miroshnikova7, Nadège Poncet1,2, Alexandra Junza10,11, Christine Kranz, Shomi S. Bhattacharya12,13, Esther Prat4,5,14, Vanita Berry12, Wolfgang Berger2,7,15, University of Ulm, Germany Elise Heon16, Anthony T. Moore12,17,18, Óscar Yanes10,11, Virginia Nunes4,5,14*‡, Reviewed by: Manuel Palacín5,6,19*‡, Francois Verrey1,2,3*‡ and Barbara Kloeckener-Gruissem7,8*‡ Snezana Petrovic, Wake Forest School of Medicine, 1 Institute of Physiology, University of Zurich, Zurich, Switzerland, 2 Zurich Center for Integrative Human Physiology, University United States of Zurich, Zurich, Switzerland, 3 Swiss National Centre of Competence in Research Kidney.CH, University of Zurich, Zurich, Yoshinori Marunaka, Switzerland, 4 Genes, Disease and Therapy Program, Molecular Genetics Laboratory – IDIBELL, Barcelona, Spain, 5 U730 Kyoto Industrial Health Association, and U731, Centro de Investigación Biomédica en Red de Enfermedades Raras, Barcelona, Spain, 6 Institute for Research Japan in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Spain, 7 Institute of Medical Molecular 8 9 *Correspondence: Genetics, University of Zurich, Zurich, Switzerland, Department of Biology, ETH Zurich, Zurich, Switzerland, Jules-Gonin 10 Virginia Nunes Eye Hospital, University of Lausanne, Lausanne, Switzerland, Metabolomics Platform, IISPV, Department of Electronic 11 [email protected] Engineering, Universitat Rovira i Virgili, Tarragona, Spain, CIBER of Diabetes and Associated Metabolic Diseases 12 Manuel Palacín (CIBERDEM), Madrid, Spain, Andalusian Molecular Biology and Regenerative Medicine Centre – CABIMER, Seville, Spain, 13 14 [email protected] UCL Institute of Ophthalmology, London, United Kingdom, Genetics Section, Department of Physiological Sciences, 15 Francois Verrey Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain, Neuroscience Center Zurich – ZNZ, 16 [email protected] University of Zurich and ETH Zurich, Zurich, Switzerland, Department of Ophthalmology and Vision Sciences, The Hospital 17 18 Barbara Kloeckener-Gruissem for Sick Children, Toronto, ON, Canada, Moorfields Eye Hospital, London, United Kingdom, Department [email protected] of Ophthalmology, School of Medicine, University of California, San Francisco, San Francisco, CA, United States, 19 Departament de Bioquímica i Biomedicina Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain †These authors have contributed equally to this work ‡These authors shared senior Cataract, the loss of ocular lens transparency, accounts for ∼50% of worldwide authorship blindness and has been associated with water and solute transport dysfunction across lens cellular barriers. We show that neutral amino acid antiporter LAT2 (Slc7a8) and Specialty section: This article was submitted to uniporter TAT1 (Slc16a10) are expressed on mouse ciliary epithelium and LAT2 also Renal and Epithelial Physiology, in lens epithelium. Correspondingly, deletion of LAT2 induced a dramatic decrease a section of the journal Frontiers in Physiology in lens essential amino acid levels that was modulated by TAT1 defect. Interestingly, Received: 27 March 2019 the absence of LAT2 led to increased incidence of cataract in mice, in particular Accepted: 16 May 2019 in older females, and a synergistic effect was observed with simultaneous lack Published: 04 June 2019 of TAT1. Screening SLC7A8 in patients diagnosed with congenital or age-related Citation: cataract yielded one homozygous single nucleotide deletion segregating in a family with Knöpfel EB, Vilches C, Camargo SMR, Errasti-Murugarren E, congenital cataract. Expressed in HeLa cells, this LAT2 mutation did not support amino Stäubli A, Mayayo C, Munier FL, acid uptake. Heterozygous LAT2 variants were also found in patients with cataract Miroshnikova N, Poncet N, Junza A, Bhattacharya SS, Prat E, Berry V, some of which showed a reduced transport function when expressed in HeLa cells. Berger W, Heon E, Moore AT, Whether heterozygous LAT2 variants may contribute to the pathology of cataract needs Yanes Ó, Nunes V, Palacín M, to be further investigated. Overall, our results suggest that defects of amino acid Verrey F and Kloeckener-Gruissem B (2019) Dysfunctional LAT2 Amino transporter LAT2 are implicated in cataract formation, a situation that may be aggravated Acid Transporter Is Associated With by TAT1 defects. Cataract in Mouse and Humans. Front. Physiol. 10:688. Keywords: amino acid transporters LAT2 and TAT1, gene expression, cataract, ocular tissues, mouse model, doi: 10.3389/fphys.2019.00688 patient screen Frontiers in Physiology| www.frontiersin.org 1 June 2019| Volume 10| Article 688 fphys-10-00688 June 3, 2019 Time: 19:57 # 2 Knöpfel et al. Amino Acid Transporter and Cataract INTRODUCTION water channel (aquaporin) (Zampighi et al., 2002) and connexin (Cx) hemichannels (Shi et al., 2018) also some solute transport Cataract, the condition of partial or complete loss of transparency proteins (Truscott, 2000). Accordingly in the lens, that includes in the ocular lens, is the most frequent cause for vision also the aqueous humor-facing lens epithelium, a variety of impairment worldwide (51%)1. Typically the condition affects transport proteins have been localized, for instance in addition individuals over 50 years of age (McCarty and Taylor, 2001) to aquaporins (Barandika et al., 2016) also ion channels (KC, and since life expectancy is increasing the number of affected NaC, Ca2C), pumps (Na,K-ATPase) (Mathias and Rae, 2004), individuals is raising, for instance in the United States from 24.4 glucose transporters (GLUT1, GLUT3, and SGLT2) (Merriman- million in 2010 it will change to potentially 50 million in 20502, Smith et al., 1999, 2003), creatine transporter CRT2 (MCT12) making age-related cataract an important socio-economical (Abplanalp et al., 2013) and amino acid transporters (GLYT problem. Age-related cataract is considered a multi-factorial AND ASCT2) (Marcantonio and Duncan, 1983, 1987; Lim et al., disease where both genetic and environmental risk factors 2006, 2007). These latter transporters need to import all amino contribute to the pathogenesis (Shiels et al., 2010; Shiels and acids involved in lens cell metabolism and in particular also Hejtmancik, 2013, 2015). In contrast,
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