Boletes Come of Age
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Boletus Edulis and Cistus Ladanifer: Characterization of Its Ectomycorrhizae, in Vitro Synthesis, and Realised Niche
UNIVERSIDAD DE MURCIA ESCUELA INTERNACIONAL DE DOCTORADO Boletus edulis and Cistus ladanifer: characterization of its ectomycorrhizae, in vitro synthesis, and realised niche. Boletus edulis y Cistus ladanifer: caracterización de sus ectomicorrizas, síntesis in vitro y área potencial. Dª. Beatriz Águeda Hernández 2014 UNIVERSIDAD DE MURCIA ESCUELA INTERNACIONAL DE DOCTORADO Boletus edulis AND Cistus ladanifer: CHARACTERIZATION OF ITS ECTOMYCORRHIZAE, in vitro SYNTHESIS, AND REALISED NICHE tesis doctoral BEATRIZ ÁGUEDA HERNÁNDEZ Memoria presentada para la obtención del grado de Doctor por la Universidad de Murcia: Dra. Luz Marina Fernández Toirán Directora, Universidad de Valladolid Dra. Asunción Morte Gómez Tutora, Universidad de Murcia 2014 Dª. Luz Marina Fernández Toirán, Profesora Contratada Doctora de la Universidad de Valladolid, como Directora, y Dª. Asunción Morte Gómez, Profesora Titular de la Universidad de Murcia, como Tutora, AUTORIZAN: La presentación de la Tesis Doctoral titulada: ‘Boletus edulis and Cistus ladanifer: characterization of its ectomycorrhizae, in vitro synthesis, and realised niche’, realizada por Dª Beatriz Águeda Hernández, bajo nuestra inmediata dirección y supervisión, y que presenta para la obtención del grado de Doctor por la Universidad de Murcia. En Murcia, a 31 de julio de 2014 Dra. Luz Marina Fernández Toirán Dra. Asunción Morte Gómez Área de Botánica. Departamento de Biología Vegetal Campus Universitario de Espinardo. 30100 Murcia T. 868 887 007 – www.um.es/web/biologia-vegetal Not everything that can be counted counts, and not everything that counts can be counted. Albert Einstein Le petit prince, alors, ne put contenir son admiration: -Que vous êtes belle! -N´est-ce pas, répondit doucement la fleur. Et je suis née meme temps que le soleil.. -
(Boletaceae, Basidiomycota) – a New Monotypic Sequestrate Genus and Species from Brazilian Atlantic Forest
A peer-reviewed open-access journal MycoKeys 62: 53–73 (2020) Longistriata flava a new sequestrate genus and species 53 doi: 10.3897/mycokeys.62.39699 RESEARCH ARTICLE MycoKeys http://mycokeys.pensoft.net Launched to accelerate biodiversity research Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest Marcelo A. Sulzbacher1, Takamichi Orihara2, Tine Grebenc3, Felipe Wartchow4, Matthew E. Smith5, María P. Martín6, Admir J. Giachini7, Iuri G. Baseia8 1 Departamento de Micologia, Programa de Pós-Graduação em Biologia de Fungos, Universidade Federal de Pernambuco, Av. Nelson Chaves s/n, CEP: 50760-420, Recife, PE, Brazil 2 Kanagawa Prefectural Museum of Natural History, 499 Iryuda, Odawara-shi, Kanagawa 250-0031, Japan 3 Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia 4 Departamento de Sistemática e Ecologia/CCEN, Universidade Federal da Paraíba, CEP: 58051-970, João Pessoa, PB, Brazil 5 Department of Plant Pathology, University of Flori- da, Gainesville, Florida 32611, USA 6 Departamento de Micologia, Real Jardín Botánico, RJB-CSIC, Plaza Murillo 2, Madrid 28014, Spain 7 Universidade Federal de Santa Catarina, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Campus Trindade – Setor F, CEP 88040-900, Flo- rianópolis, SC, Brazil 8 Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte, Campus Universitário, CEP: 59072-970, Natal, RN, Brazil Corresponding author: Tine Grebenc ([email protected]) Academic editor: A.Vizzini | Received 4 September 2019 | Accepted 8 November 2019 | Published 3 February 2020 Citation: Sulzbacher MA, Orihara T, Grebenc T, Wartchow F, Smith ME, Martín MP, Giachini AJ, Baseia IG (2020) Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest. -
AR TICLE New Sequestrate Fungi from Guyana: Jimtrappea Guyanensis
IMA FUNGUS · 6(2): 297–317 (2015) doi:10.5598/imafungus.2015.06.02.03 New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., ARTICLE Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales) Matthew E. Smith1, Kevin R. Amses2, Todd F. Elliott3, Keisuke Obase1, M. Catherine Aime4, and Terry W. Henkel2 1Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA 2Department of Biological Sciences, Humboldt State University, Arcata, CA 95521, USA; corresponding author email: Terry.Henkel@humboldt. edu 3Department of Integrative Studies, Warren Wilson College, Asheville, NC 28815, USA 4Department of Botany & Plant Pathology, Purdue University, West Lafayette, IN 47907, USA Abstract: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus Key words: cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected Boletineae in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Caesalpinioideae Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea Dipterocarpaceae (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) ectomycorrhizal fungi and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and gasteroid fungi micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Guiana Shield Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera. Article info: Submitted: 31 May 2015; Accepted: 19 September 2015; Published: 2 October 2015. INTRODUCTION 2010, Gube & Dorfelt 2012, Lebel & Syme 2012, Ge & Smith 2013). -
Sequestrate Boletaceae)
PERSOONIA Volume 18, Part 4,499-504(2005) New observations on the basidiome ontogeny of Chamonixia caespitosa (sequestrate Boletaceae) H. Cleménçon Institut d'Ecologie, Universite de Lausanne, CH-1015 Lausanne, Switzerland E-mail: [email protected] The description of basidiome development of Chamonixia caespitosa made by Eduard Fischer during the first quarter of the last century is extended, based on a new investigation of the original permanent mounts. This puffball-like fungus is A exocarpic, claustropileate and amphicleistoblemate. hymeniform palisade on the primordial stipe becomes partly covered and obliterated by the pileus margin and an amphicleistoblema. The morphological data confirm the molecular-taxonomic position of Chamonixia in the Boletaceae. Chamonixiacaespitosa Rolland is a puffball-like Basidiomycete with a finely tomen- tose peridium turning blue when bruised. Molecular analyses showed that the genus Chamonixia has phylogenetic affinities with the boletes (Bruns et al., 1998;Kretzer & Bruns, 1999). In 1925 the Swiss mycologist Eduard Fischer published a description ofthe fruit- body development of C. caespitosa based on thick sections that he made from material collected by E. Soehner (1922, as Hymenogaster caerulescens Soehner). He strongly resemblance of the of Chamonixia with emphasised the early stages caespitosa early of with free such stages gymnocarpic agarics a pileus margin, as Gymnopus dryophilus (Collybia dryophila), and he explained the puffball-like appearance of the mature basid- iome with the fact that the pileus margin grows towards the primordial stipe and fuses with it, whilethe hymenophore, instead of being regular as in agarics, becomes sponge-like (Fig. 1). Therefore, Fischer (1925) called the development of C. -
Systematics of the Genus Rhizopogon Inferred from Nuclear Ribosomal DNA Large Subunit and Internal Transcribed Spacer Sequences
AN ABSTRACT OF THE THESIS OF Lisa C. Grubisha for the degree of Master of Science in Botany and Plant Pathology presented on June 22, 1998. Title: Systematics of the Genus Rhizopogon Inferred from Nuclear Ribosomal DNA Large Subunit and Internal Transcribed Spacer Sequences. Abstract approved Redacted for Privacy Joseph W. Spatafora Rhizopogon is a hypogeous fungal genus that forms ectomycorrhizae with genera of the Pinaceae. The greatest number and species of Rhizopogon are found in coniferous forests of the Pacific Northwestern United States, where members of the Pinaceae are also concentrated. Rhizopogon spp. are host-specific primarily with Pinus spp. and Pseudotsuga spp. and thus are an important component of these forest ecosystems. Rhizopogon includes over 100 species; however, the systematics of Rhizopogon have not been well understood. Currently the genus is placed in the Boletales, an order of ectomycorrhizal fungi that are primarily epigeous and have a tubular hymenium. Suillus is a stipitate genus closely related to Rhizopogon that is also in the Boletales and host specific with Pinaceae.I examined the relationship of Rhizopogon to Suillus and other genera in the Boletales. Infrageneric relationships in Rhizopogon were also investigated to test current taxonomic hypotheses and species concepts. Through phylogenetic analyses of large subunit and internal transcribed spacer nuclear ribosomal DNA sequences, I found that Rhizopogon and Suillus formed distinct monophyletic groups. Rhizopogon was composed of four distinct groups; sections Amylopogon and Villosuli were strongly supported monophyletic groups. Section Rhizopogon was not monophyletic, and formed two distinct clades. Section Fulviglebae formed a strongly supported group within section Villosuli. -
New Localities of Chamonixia Caespitosa (Hypogeous Boletaceae) in Central Europe
ACTa MYCOLoGica Vol. 44 (1): 29–42 2009 New localities of Chamonixia caespitosa (hypogeous Boletaceae) in Central Europe PIOTR MLECZKO1, MACIEJ KOZAK1, Maria Ławrynowicz2 and anna GÓrSzczyK3 1Institute of Botany, Jagiellonian University, Lubicz 46 PL-31-512 Kraków, [email protected] 2Department of Mycology, University of Łódź Banacha 12/16, PL-90-237 Łódź, [email protected] 3Orzeszkowej 6, PL-32-640 Zator Mleczko P., Kozak M., Ławrynowicz M., Górszczyk a.: New localities of Chamonixia caespitosa (hypogeous Boletaceae) in Central Europe. acta Mycol. 44 (1): 29–42, 2009. Chamonixia caespitosa rolland, has been recently found in Poland for the first time after 1945. The basidiocarps, partially exposed from the humus layer, were found in two localities: in the spruce forest in the Polish Tatra Mts., at the elevation of 1540 m a.s.l., and in the mixed forest with spruce and fir in the Beskid niski Mts. at the elevation of app. 400 m a.s.l. The description of the Polish specimens generally agrees with descriptions of the specimens found in other Central European countries. The roundish to tuberculate basidiocarps were characterized by the presence of highly reduced stipe, whitish colour of the peridium changing rapidly to blue after exposure to air, small, complete or incomplete columella and brown, spongy gleba. Typically 4-spored basidia were present which produced ellipsoid, brown spores with the ornamentation in the form of rough, interconnected ridges. Taxonomic position, ecology and chorology of the species, the ontogeny of basidiocarps and description of ectomycorrhizae are summarized in the paper. Key words: hypogeous fungi, ectomycorrhizal fungi, Boletales, Tatra Mts., Beskid niski Mts. -
Boletales – Boletaceae S.L. (26 October 2020, © R. E. Halling)
Boletales – Boletaceae s.l. (26 October 2020, © R. E. Halling) NOTE: 104 genera listed here are conceived in a broad, classical sense (generally the fleshy stipitate mushrooms with pores) including sequestrate morphologies. Phylogenetic inferences from DNA sequences suggest alignment in suborders: Boletineae, Suillineae, Sclerodermatineae, or in the Paxillaceae. Not all genera are well known, equally circumscribed or robustly inferred phylogenetically. Mycorrhizal associations may be confirmed, but many are presumed or suspected. Recent phylogenetic analyses based on DNA sequences infer some true gasteroid (truffle-like, sequestrate) taxa (aside from those in Sclerodermatineae, Suillineae) belong here. Some of the diagnoses are from protologues. Year of publication follows authority (-ies). Afroboletus Pegler & Young (1981) Pileus dry, coarsely fibrillose to squamose, black, often with appendiculate veil remnants, microscopically a trichodermium. Context white, staining red then black. Hymenophore adnexed, white then black, staining red then black. Peronate veil present. Stipe dry, squamose, sometimes annulate, white to gray to black. Spores black, short ellipsoid, longitudinally ridged or winged, sometimes with intercostal veins; a basal thickened rim around sterigmal appendage, lacking a plage. Hymenial cystidia present. Clamp connections absent. Apparently restricted to the African tropics. One sequestrate species known. Ectomycorrhizae presumed with caesalpinoid legumes. Afrocastellanoa M.E. Smith & Orihara (2017) From the protologue: Basidiomata sequestrate, gasteroid, firm, rubbery, with one or a few rhizomorphs at the base. Similar to Octaviania in the morphology of the basidiome and basidiospores, but different from Octaviania in the multilayered peridium and in basidia that are irregularly distributed within the solid gleba, resulting in the absence of a distinct hymenium and subhymenium. -
Revision of Leccinoid Fungi, with Emphasis on North American Taxa
MYCOLOGIA 2020, VOL. 112, NO. 1, 197–211 https://doi.org/10.1080/00275514.2019.1685351 Revision of leccinoid fungi, with emphasis on North American taxa, based on molecular and morphological data Michael Kuo a and Beatriz Ortiz-Santana b aThe Herbarium of Michael Kuo, P.O. Box 742, Charleston, Illinois 61920; bCenter for Forest Mycology Research, Northern Research Station, United States Department of Agriculture Forest Service, One Gifford Pinchot Drive, Madison, Wisconsin 53726 ABSTRACT ARTICLE HISTORY The leccinoid fungi are boletes and related sequestrate mushrooms (Boletaceae, Basidiomycota) Received 30 April 2019 that have traditionally been placed in Leccinum, Boletus, Leccinellum, and a handful of other less Accepted 23 October 2019 familiar genera. These mushrooms generally feature scabers or scaber-like dots on the surface of KEYWORDS the stipe, and they are often fairly tall and slender when compared with other boletes. They are Basidiomycota; Boletaceae; ectomycorrhizal fungi and appear to be fairly strictly associated with specific trees or groups of Octaviania; Chamonixia; related trees. In the present study, we investigate the phylogenetic relationships among the Leccinellum; Leccinum; leccinoid fungi and other members of the family Boletaceae using portions of three loci from Rossbeevera; Turmalinea;10 nuc 28S rDNA (28S), translation elongation factor 1-α (TEF1), and the RNA polymerase II second- new taxa largest subunit (RPB2). Two DNA data sets (combined 28S-TEF1 and 28S-TEF1-RPB2), comprising sequences from nearly 270 voucher specimens, were evaluated using two different phylogenetic analyses (maximum likelihood and Bayesian inference). Five major clades were obtained, and leccinoid fungi appeared in four of them. -
Complete References List
Aanen, D. K. & T. W. Kuyper (1999). Intercompatibility tests in the Hebeloma crustuliniforme complex in northwestern Europe. Mycologia 91: 783-795. Aanen, D. K., T. W. Kuyper, T. Boekhout & R. F. Hoekstra (2000). Phylogenetic relationships in the genus Hebeloma based on ITS1 and 2 sequences, with special emphasis on the Hebeloma crustuliniforme complex. Mycologia 92: 269-281. Aanen, D. K. & T. W. Kuyper (2004). A comparison of the application of a biological and phenetic species concept in the Hebeloma crustuliniforme complex within a phylogenetic framework. Persoonia 18: 285-316. Abbott, S. O. & Currah, R. S. (1997). The Helvellaceae: Systematic revision and occurrence in northern and northwestern North America. Mycotaxon 62: 1-125. Abesha, E., G. Caetano-Anollés & K. Høiland (2003). Population genetics and spatial structure of the fairy ring fungus Marasmius oreades in a Norwegian sand dune ecosystem. Mycologia 95: 1021-1031. Abraham, S. P. & A. R. Loeblich III (1995). Gymnopilus palmicola a lignicolous Basidiomycete, growing on the adventitious roots of the palm sabal palmetto in Texas. Principes 39: 84-88. Abrar, S., S. Swapna & M. Krishnappa (2012). Development and morphology of Lysurus cruciatus--an addition to the Indian mycobiota. Mycotaxon 122: 217-282. Accioly, T., R. H. S. F. Cruz, N. M. Assis, N. K. Ishikawa, K. Hosaka, M. P. Martín & I. G. Baseia (2018). Amazonian bird's nest fungi (Basidiomycota): Current knowledge and novelties on Cyathus species. Mycoscience 59: 331-342. Acharya, K., P. Pradhan, N. Chakraborty, A. K. Dutta, S. Saha, S. Sarkar & S. Giri (2010). Two species of Lysurus Fr.: addition to the macrofungi of West Bengal. -
Evolutionary History of the Sequestrate Genus Rossbeevera (Boletaceae) Reveals a New Genus Turmalinea and Highlights the Utility
Persoonia 37, 2016: 173–198 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158516X691212 Evolutionary history of the sequestrate genus Rossbeevera (Boletaceae) reveals a new genus Turmalinea and highlights the utility of ITS minisatellite-like insertions for molecular identification T. Orihara1, T. Lebel 2, Z.-W. Ge3, M.E. Smith 4, N. Maekawa 5 Key words Abstract The sequestrate (truffle-like) basidiomycete genera Rossbeevera, Chamonixia, and Octaviania are closely related to the epigeous mushroom genera Leccinum and Leccinellum. In order to elucidate the properties and place- biogeography ment of several undescribed sequestrate taxa in the group and to reveal the evolutionary history of Rossbeevera and cryptic species its allies, we conducted phylogenetic analyses based on three nuclear (ITS, nLSU, EF-1α) and two mitochondrial DNA barcoding DNA loci (ATP6 and mtSSU) as well as precise morphological observations. Phylogenetic analyses of three nuclear hypogeous fungi loci suggest a complex evolutionary history with sequestrate fruiting bodies present in several clades, including a introgression previously unrecognized sister clade to Rossbeevera. Here we propose a new sequestrate genus, Turmalinea, with species tree four new species and one new subspecies as well as two new species of Rossbeevera. The three-locus nuclear phylogeny resolves species-level divergence within the Rossbeevera-Turmalinea lineage, whereas a separate phylogeny based on two mitochondrial genes corresponds to geographic distance within each species-level line- age and suggests incomplete lineage sorting (ILS) and gene introgression within several intraspecific lineages of Rossbeevera. Furthermore, topological incongruence among the three nuclear single-locus phylogenies suggests that ancient speciation within Rossbeevera probably involved considerable ILS. -
Studies on Secotiaceous Fungi IV. Gastroboletus
STUDIES ON SECOTIACEOUS FUNGI-IV GASTROBOLETUS, TRUNCOCOLUMELLA AND CHAMONIXIA ALEXANDER H. SMITH AND ROLF SINGER! University of Michigan, Ann Arbor, and Universidad Nacional de Tucum{lII, Tucum{lIl, Argentina MalenQon (1931) showed that a series of related genera connected Lactal'ius and Russula of the Agaricales to certain genera of the Gastromycetes, including some hypogeous genera. He us~d the name "La Serie des Asterospol'es" for this group. The present paper deals with a second, analogous series which we here designate the"Gctstroboletus Series." At the level of the Hymenom;ycetes this series includes the Boletales in the sense of Singer (1951). At the secotioid level in the Gastromycetes it includes Gastl'obolet'tts. Tl'uncocolmnella and Rhizopo{jon represent progressively more gastromycetous genera. \Ve regard ChanlOnixia as bt'longing to this "eries at the level of TnlllcocolulHclla. Rhizo- 1 Papers from the University Herbarium and the Dcpnrtment. of Botnn~' 1\o. 1081. Univer sity of ?'fichig:ul. BRI'I"rOXIA 11: 205-223. October 1959. 206 nRlTTONJA [\'OL. 1] ])o[lon is not treated here because it is sudl a large genus that it represt'llts H major study in itself. Howrver, we should like to emphasize here that a redsiolJ of that genus in the light of our series of papers on secotioid fungi is now urgently needed to clarify the trends of evolution among hypogeous 13a"idio. mycetes. One other genus, Gauticria, eventually may be found to belong to thi~ series, but we are not including' it in this paper. By its spore characters it resembles Cha11l01l1:xia, but it differs in the gelatinous-cartilaginous texture of the tissues of the gastrocarp. -
New Localities of Chamonixia Caespitosa (Hypogeous Boletaceae) in Central Europe
ACTa MYCOLoGica Vol. 44 (1): 29–42 2009 New localities of Chamonixia caespitosa (hypogeous Boletaceae) in Central Europe PIOTR MLECZKO1, MACIEJ KOZAK1, Maria Ławrynowicz2 and anna GÓrSzczyK3 1Institute of Botany, Jagiellonian University, Lubicz 46 PL-31-512 Kraków, [email protected] 2Department of Mycology, University of Łódź Banacha 12/16, PL-90-237 Łódź, [email protected] 3Orzeszkowej 6, PL-32-640 Zator Mleczko P., Kozak M., Ławrynowicz M., Górszczyk a.: New localities of Chamonixia caespitosa (hypogeous Boletaceae) in Central Europe. acta Mycol. 44 (1): 29–42, 2009. Chamonixia caespitosa rolland, has been recently found in Poland for the first time after 1945. The basidiocarps, partially exposed from the humus layer, were found in two localities: in the spruce forest in the Polish Tatra Mts., at the elevation of 1540 m a.s.l., and in the mixed forest with spruce and fir in the Beskid niski Mts. at the elevation of app. 400 m a.s.l. The description of the Polish specimens generally agrees with descriptions of the specimens found in other Central European countries. The roundish to tuberculate basidiocarps were characterized by the presence of highly reduced stipe, whitish colour of the peridium changing rapidly to blue after exposure to air, small, complete or incomplete columella and brown, spongy gleba. Typically 4-spored basidia were present which produced ellipsoid, brown spores with the ornamentation in the form of rough, interconnected ridges. Taxonomic position, ecology and chorology of the species, the ontogeny of basidiocarps and description of ectomycorrhizae are summarized in the paper. Key words: hypogeous fungi, ectomycorrhizal fungi, Boletales, Tatra Mts., Beskid niski Mts.