Evaluation of the Hypervariable Region in the 16S Rdna Sequence As an Index for Rapid Species Identification in the Genus Paenibacillus
J. Gen. Appl. Microbiol., 48, 281–285 (2002) Short Communication Evaluation of the hypervariable region in the 16S rDNA sequence as an index for rapid species identification in the genus Paenibacillus Keiichi Goto,* Yuko Kato, Mika Asahara, and Akira Yokota1 Microbiological & Analytical Group, Food Research Laboratories, Mitsui Norin Co., Ltd., Fujieda 426–0133, Japan 1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113–0032, Japan (Received January 9, 2002; Accepted August 29, 2002) Key Words——Bacillus; grouping; hypervariable region; identification; Paenibacillus; 16S rRNA gene The genus Paenibacillus (Ash et al., 1993) compris- additional strains of Paenibacillus species were sub- ing aerobic or facultatively anaerobic endospore-form- jected to a sequence comparison of the HV regions ing bacilli is represented by rRNA group 3 and con- and the use of this HV region for molecular typing of tains 28 validated species and 2 subspecies. The the various species of the genus Paenibacillus was genus is phenotypically similar to other members of evaluated. the family Bacillaceae but is difficult to distinguish from The 59 strains used in this study were obtained from the other genera by morphology alone. Recently a ATCC (American Type Culture Collection, Manassas, highly specific primer set directed to the 16S rDNA of VA, USA), DSM (Deutsche Sammlung von Mikroor- Paenibacillus was described (Shida et al., 1997), ganismen und Zellkulturen GmbH, Braunschweig, Ger- which enabled the rapid identification of Paenibacillus many), IAM (Institute of Molecular and Cellular Bio- to the genus level. However the identification of Paeni- sciences, The University of Tokyo, Tokyo, Japan), IFO bacillus below the genus level remains difficult and (Institute for Fermentation, Osaka, Japan) and NCIMB time-consuming due to the absence of well-defined (National Collection of Industrial and Marine Bacteria, phenotypes that differentiate species (Reva et al., Aberdeen, UK).
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