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DATASHEET USA: [email protected] FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] OXCT1 Polyclonal Anbody Catalog number: 12175-1-AP Background Size: 38 μg/150 μl 3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial Source: Rabbit CoA transferase required for ketone body degradaon. It catalyzes the transfer of Isotype: IgG CoA from succinyl-CoA to acetoacetate, generang acetoacetyl-CoA. OXCT1 is Synonyms: expressed in brain, heart, and skeletal muscle, but not in liver. This anbody OXCT1; 3 oxoacid CoA specifically recognizes endogenous OXCT1. (21209089) transferase 1, OXCT, OXCT1, SCOT, SCOT s Applications Tested applicaons: ELISA, WB, IHC, IF, IP Cited applicaons: IHC, WB Species specificity: Human,Mouse,Rat; other species not tested. Cited species: Human, mouse Caculated OXCT1 MW: 520aa,56 kDa Observed OXCT1 MW: 56 kDa Posive WB detected in Human brain ssue, human heart ssue, human kidney Immunofluorescent analysis of MCF-7 ssue, human lung ssue, mouse skeletal muscle ssue, cells, using OXCT1 anbody 12175-1- AP at 1:25 diluon and Rhodamine- mouse thymus ssue labeled goat an-rabbit IgG (red). Posive IP detected in Mouse brain ssue Posive IHC detected in Human medulloblastoma ssue Posive IF detected in MCF-7 cells Recommended diluon: WB: 1:1000-1:10000 IP: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:10-1:100 Applicaon key: WB = Western blong, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipitaon Immunohistochemical of paraffin- embedded human medulloblastoma Immunogen information using 12175-1-AP(SCOT anbody) at diluon of 1:50 (under 10x lens) Immunogen: Ag2818 GenBank accession number: BC009001 Gene ID (NCBI): 5019 Full name: 3-oxoacid CoA transferase 1 Product information Purificaon method: Angen affinity purificaon Storage: PBS with 0.1% sodium azide and 50% glycerol pH o human brain ssue were subjected to 7.3. Store at -20 C. SDS PAGE followed by western blot with 12175-1-AP(SCOT anbody) at diluon of 1:400 IP Result of an-SCOT (IP:12175-1-AP, 3ug; Detecon:12175-1-AP 1:1000) Detecon:12175-1-AP 1:1000) with mouse brain ssue lysate 4000ug..

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A Turkish Patient with Succinyl-Coa:3-Oxoacid Coa
Original Article Journal of Inborn Errors of Metabolism & Screening 2016, Volume 4: 1–5 A Turkish Patient With ª The Author(s) 2016 DOI: 10.1177/2326409816651281 Succinyl-CoA:3-Oxoacid CoA iem.sagepub.com Transferase Deficiency Mimicking Diabetic Ketoacidosis Sahin Erdol, MD1, Mehmet Ture, MD2, Tahsin Yakut, PhD2, Halil Saglam, PhD1, Hideo Sasai, MD3, Elsayed Abdelkreem, MD3, Hiroki Otsuka, MD3, and Toshiyuki Fukao, MD, PhD3 Abstract Succinyl-CoA:3-oxoacid CoA transferase (SCOT) deficiency is an autosomal recessive disorder of ketone body utilization that is clinically characterized with intermittent ketoacidosis crises. We report here the second Turkish case with SCOT deficiency. She experienced 3 ketoacidotic episodes: The first ketoacidotic crisis mimicked diabetic ketoacidosis because of the associated hyperglycemia. Among patients with SCOT deficiency, the blood glucose levels at the first crises were variable, and this case had the highest ever reported blood glucose level. She is a compound heterozygote with 2 novel mutations, c.517A>G (K173E) and c.1543A>G (M515V), in exons 5 and 17 of the OXCT1 gene, respectively. In patient’s fibroblasts, SCOT activity was deficient and, by immunoblot analysis, SCOT protein was much reduced. The patient attained normal development and had no permanent ketosis. The accurate diagnosis of SCOT deficiency in this case had a vital impact on the management strategy and outcome. Keywords succinyl-CoA:3-oxoacid CoA transferase, deficiency, mimicking, diabetic ketoacidosis, Turkey Introduction deficiency. We herein describe a Turkish SCOT-deficient patient whose first episode mimicked diabetic ketoacidosis Succinyl-CoA:3-oxoacid CoA transferase (SCOT) deficiency because of the associated hyperglycemia.
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Novel Compound Heterozygous OXCT1 Mutations Causing Succinyl-Coa:3-Ketoacid Coa Transferase Deficiency
Case Report Yonsei Med J 2019 Mar;60(3):308-311 https://doi.org/10.3349/ymj.2019.60.3.308 pISSN: 0513-5796 · eISSN: 1976-2437 A Rare Cause of Life-Threatening Ketoacidosis: Novel Compound Heterozygous OXCT1 Mutations Causing Succinyl-CoA:3-Ketoacid CoA Transferase Deficiency Young A Kim1,2, Seong Heon Kim1, Chong Kun Cheon1, and Yoo-Mi Kim3 1Department of Pediatrics, Pusan National University Children’s Hospital, Yangsan; 2Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan; 3Department of Pediatrics, Chungnam National University Hospital, College of Medicine, Chungnam National University, Daejeon, Korea. Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency is a rare inborn error of ketone body utilization, characterized by ep- isodic or permanent ketosis. SCOT deficiency is caused by mutations in the OXCT1 gene, which is mapped to 5p13 and consists of 17 exons. A 12-month-old girl presented with severe ketoacidosis and was treated with continuous renal replacement therapy. She had two previously unrecognized mild-form episodes of ketoacidosis followed by febrile illness. While high levels of ketone bodies were found in her blood and urine, other laboratory investigations, including serum glucose, were unremarkable. We identified novel compound heterozygous mutations in OXCT1:c.1118T>G (p.Ile373Ser) and a large deletion ranging from exon 8 to 16 through targeted exome sequencing and microarray analysis. This is the first Korean case of SCOT deficiency caused by novel mutations in OXCT1, resulting in life-threatening ketoacidosis. In patients with unexplained episodic ketosis, or high anion gap metabolic acidosis in infancy, an inherited disorder in ketone body metabolism should be suspected.
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Anti-OXCT1 Antibody (ARG58973)
Product datasheet [email protected] ARG58973 Package: 100 μl anti-OXCT1 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes OXCT1 Tested Reactivity Hu, Ms, Rat Tested Application IHC-P, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name OXCT1 Antigen Species Human Immunogen Recombinant fusion protein corresponding to aa. 261-520 of Human OXCT1 (NP_000427.1). Conjugation Un-conjugated Alternate Names OXCT; EC 2.8.3.5; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT-s; SCOT; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; 3-oxoacid CoA-transferase 1 Application Instructions Application table Application Dilution IHC-P 1:50 - 1:100 WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control Mouse heart Calculated Mw 56 kDa Observed Size 56 kDa Properties Form Liquid Purification Affinity purified. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol OXCT1 Gene Full Name 3-oxoacid CoA transferase 1 Background This gene encodes a member of the 3-oxoacid CoA-transferase gene family.
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Succinyl-Coa:3-Ketoacid Coa Transferase Deficiency
Succinyl-CoA:3-ketoacid CoA transferase deficiency Description Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency is an inherited disorder that impairs the body's ability to break down ketones, which are molecules produced in the liver during the breakdown of fats. The signs and symptoms of SCOT deficiency typically appear within the first few years of life. Affected individuals experience episodes of extreme tiredness (lethargy), appetite loss, vomiting, rapid breathing, and, occasionally, seizures. These episodes, which are called ketoacidotic attacks, sometimes lead to coma. About half of affected individuals have a ketoacidotic attack within the first 4 days of life. Affected individuals have no symptoms of the disorder between ketoacidotic attacks. People with SCOT deficiency usually have a permanently elevated level of ketones in their blood (persistent ketosis). If the level of ketones gets too high, which can be brought on by infections, fevers, or periods without food (fasting), a ketoacidotic attack can occur. The frequency of ketoacidotic attacks varies among affected individuals. Frequency The prevalence of SCOT deficiency is unknown. More than 20 cases of this condition have been reported in the scientific literature. Causes Mutations in the OXCT1 gene cause SCOT deficiency. The OXCT1 gene provides instructions for making an enzyme called succinyl-CoA:3-ketoacid CoA transferase ( SCOT). The SCOT enzyme is made in the energy-producing centers of cells ( mitochondria). The enzyme plays a role in the breakdown of ketones, which are an important source of energy during fasting or when energy demands are increased, such as during illness or when exercising. OXCT1 gene mutations result in the production of a SCOT enzyme with little or no function.
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Regulation of Autophagy by Acetyl Coenzime a : from the Mechanisms to a Revised Definition of Caloric Restriction Mimetics Federico Pietrocola
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EGL Test Description
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