DATASHEET USA: [email protected] FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] OXCT1 Polyclonal Anbody Catalog number: 12175-1-AP Background Size: 38 μg/150 μl 3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial Source: Rabbit CoA transferase required for ketone body degradaon. It catalyzes the transfer of Isotype: IgG CoA from succinyl-CoA to acetoacetate, generang acetoacetyl-CoA. OXCT1 is Synonyms: expressed in brain, heart, and skeletal muscle, but not in liver. This anbody OXCT1; 3 oxoacid CoA specifically recognizes endogenous OXCT1. (21209089) transferase 1, OXCT, OXCT1, SCOT, SCOT s Applications Tested applicaons: ELISA, WB, IHC, IF, IP Cited applicaons: IHC, WB Species specificity: Human,Mouse,Rat; other species not tested. Cited species: Human, mouse Caculated OXCT1 MW: 520aa,56 kDa Observed OXCT1 MW: 56 kDa Posive WB detected in Human brain ssue, human heart ssue, human kidney Immunofluorescent analysis of MCF-7 ssue, human lung ssue, mouse skeletal muscle ssue, cells, using OXCT1 anbody 12175-1- AP at 1:25 diluon and Rhodamine- mouse thymus ssue labeled goat an-rabbit IgG (red). Posive IP detected in Mouse brain ssue Posive IHC detected in Human medulloblastoma ssue Posive IF detected in MCF-7 cells Recommended diluon: WB: 1:1000-1:10000 IP: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:10-1:100 Applicaon key: WB = Western blong, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipitaon Immunohistochemical of paraffin- embedded human medulloblastoma Immunogen information using 12175-1-AP(SCOT anbody) at diluon of 1:50 (under 10x lens) Immunogen: Ag2818 GenBank accession number: BC009001 Gene ID (NCBI): 5019 Full name: 3-oxoacid CoA transferase 1 Product information Purificaon method: Angen affinity purificaon Storage: PBS with 0.1% sodium azide and 50% glycerol pH o human brain ssue were subjected to 7.3. Store at -20 C. SDS PAGE followed by western blot with 12175-1-AP(SCOT anbody) at diluon of 1:400 IP Result of an-SCOT (IP:12175-1-AP, 3ug; Detecon:12175-1-AP 1:1000) Detecon:12175-1-AP 1:1000) with mouse brain ssue lysate 4000ug..