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Appendix 1: Leptospira proteins identified in Leptospira interrogans serovar Canicola Accession Number Protein identification Protein Length (Amino Acids) DNA polymerase III 24212702 373 subunit beta 24212705 DNA gyrase subunit B 639 24212706 DNA gyrase subunit A 834 hypothetical protein 24212709 245 LA_0009 short chain 24212720 248 dehydrogenase 24212721 translation factor Sua5 203 hypothetical protein 24212731 234 LA_0031 inositol 24212733 monophosphatase family 283 protein 24212740 ribonuclease R 748 TPR-repeat-containing 24212743 502 protein 3-dehydroquinate 24212745 234 dehydratase nucleoside-diphosphate- 24212751 312 sugar epimerase NAD(P)(+) 24212752 transhydrogenase 389 subunit alpha 24212754 histidyl-tRNA synthetase 439 24212756 OmpA family protein 687 deoxycytidine 24212772 174 triphosphate deaminase diaminopimelate 24212783 281 epimerase hypothetical protein 24212807 118 LA_0107 cystathionine gamma- 24212813 500 synthase imidazoleglycerol- phosphate 24212824 206 dehydratase/histidinol- phosphatase phosphoribosylformimin o-5-aminoimidazole 24212826 241 carboxamide ribotide isomerase hypothetical protein 24212827 404 LA_0127 phosphomethylpyrimidin 24212830 274 e kinase protein ribosome-associated 24212834 318 GTPase hypothetical protein 24212835 433 LA_0135 metal-dependent 24212841 hydrolase of the beta- 312 lactamase superfamily I 24212846 transcriptional regulator 699 SET domain-containing 24212849 133 protein hypothetical protein 24212853 489 LA_0153 24212857 chorismate synthase 380 NADH dehydrogenase 24212860 482 (ubiquinone) chain G preprotein translocase 24212878 627 subunit YidC Jag-like RNA-binding 24212879 236 protein hypothetical protein 24212884 692 LA_0184 24212885 fumarate hydratase 464 hypothetical protein 24212918 510 LA_0218 24212922 OmpA family lipoprotein 195 cytochrome c oxidase 24212943 534 polypeptide I integral membrane 24212950 343 protein with PIN domain phosphoenolpyruvate 24212951 530 carboxykinase aspartyl/glutamyl-tRNA 24212956 amidotransferase 486 subunit B hypothetical protein 24212963 242 LA_0263 hypothetical protein 24212972 337 LA_0272 hypothetical protein 24212978 94 LA_0278 24212980 cAMP-binding protein 405 hypothetical protein 24212984 231 LA_0284 ADP-ribose 24213000 182 pyrophosphatase metal-dependent 24213011 416 hydrolase 24213013 elongation factor G 706 NAD(P)(+) 24213035 transhydrogenase 466 subunit beta 24213060 glycine dehydrogenase 964 hypothetical protein 24213065 553 LA_0365 ribose-5-phosphate 24213069 145 isomerase ATP-dependent protease 24213091 846 ClpA phosphohistidine 24213101 160 phosphatase ABC transporter ATP- 24213102 304 binding protein tryptophanyl-tRNA 24213108 322 synthetase electron transfer 24213111 flavoprotein subunit 319 alpha electron transfer 24213112 254 flavoprotein subunit beta isovaleryl-CoA 24213114 394 dehydrogenase hypothetical protein 24213119 182 LA_0419 24213138 acyl-CoA dehydrogenase 565 hypothetical protein 24213147 56 LA_0447 acetyl-CoA 24213157 393 acetyltransferase riboflavin synthase 24213163 151 subunit beta isopropylmalate/homocit 24213169 rate/citramalate 428 synthase hypothetical protein 24213171 157 LA_0471 hypothetical protein 24213194 231 LA_0494 hypothetical protein 24213205 366 LA_0505 UDP-3-O-[3- hydroxymyristoyl] 24213213 340 glucosamine N- acyltransferase pyridoxal phosphate- 24213233 dependent 369 aminotransferase hypothetical protein 24213244 391 LA_0544 hypothetical protein 24213246 841 LA_0546 periplasmic solute- 24213252 338 binding protein glutathione S-transferase- 24213295 209 like protein 24213311 cell division protein FtsA 409 shikimate 5- 24213338 313 dehydrogenase 24213346 30S ribosomal protein S1 384 anti-sigma regulatory 24213348 222 factor excinuclease ABC subunit 24213349 666 B mannose-6-phosphate 24213356 330 isomerase metallo-beta-lactamase 24213365 321 superfamily protein 24213371 citrate synthase 426 24213374 oxidoreductase 514 24213393 aspartate kinase 405 hypothetical protein 24213420 249 LA_0720 carbamoyl phosphate 24213427 1106 synthase large subunit peroxiredoxin-like 24213434 183 protein 24213437 elongation factor Tu 401 30S ribosomal protein 24213438 102 S10 24213439 50S ribosomal protein L3 206 24213440 50S ribosomal protein L4 211 50S ribosomal protein 24213441 104 L23 24213442 50S ribosomal protein L2 279 50S ribosomal protein 24213444 110 L22 24213445 30S ribosomal protein S3 225 50S ribosomal protein 24213446 137 L16 24213451 50S ribosomal protein L5 182 30S ribosomal protein 24213452 61 S14 24213453 30S ribosomal protein S8 133 24213454 50S ribosomal protein L6 179 50S ribosomal protein 24213455 122 L18 50S ribosomal protein 24213458 180 L15 24213460 adenylate kinase 187 30S ribosomal protein 24213462 125 S13 24213464 30S ribosomal protein S4 207 RNA polymerase subunit 24213465 325 alpha 50S ribosomal protein 24213466 171 L17 L,L-diaminopimelate 24213476 408 aminotransferase aspartate 24213478 carbamoyltransferase 320 catalytic subunit 24213485 Zn-dependent hydrolase 347 SAM-dependent 24213488 315 methyltransferase cell shape determination 24213499 146 protein pyridoxal phosphate 24213500 352 biosynthesis protein trypsin-like serine 24213509 388 protease Holliday junction DNA 24213510 341 helicase B 24213527 thioesterase 286 24213533 pantothenate kinase 257 UDP-N- 24213538 acetylmuramoylalanine-- 463 D-glutamate ligase anti-sigma factor 24213539 110 antagonist indole-3-glycerol- 24213540 252 phosphate synthase 2-isopropylmalate 24213545 298 synthase 50S ribosomal protein 24213549 104 L21 50S ribosomal protein 24213551 84 L27 24213552 GTPase ObgE 357 24213553 gamma-glutamyl kinase 290 gamma-glutamyl 24213554 416 phosphate reductase nicotinate-nucleotide 24213555 199 adenylyltransferase 24213562 thiol peroxidase 171 cytoplasmic membrane 24213573 423 protein hypothetical protein 24213579 156 LA_0879 NADH dehydrogenase 24213590 443 (ubiquinone) chain F NADH dehydrogenase 24213592 405 (ubiquinone) chain D NADH dehydrogenase 24213593 178 (ubiquinone) chain C MaoC-like acyl 24213599 154 dehydratase MarR family 24213600 151 transcriptional regulator 24213603 methylase 543 24213609 methylglyoxal synthase 149 24213613 NHL repeat protein 676 24213618 enoyl-CoA hydratase 266 24213629 enolase-phosphatase 234 hypothetical protein 24213640 392 LA_0940 transcription elongation 24213642 460 factor NusA polynucleotide 24213647 699 phosphorylase hypothetical protein 24213654 441 LA_0954 glutamate synthase 24213656 1498 subunit alpha thiamine biosynthesis 24213680 495 protein ThiC 24213707 glutathione peroxidase 189 transcription termination 24213721 482 factor Rho diaminopimelate 24213747 422 decarboxylase hypothetical protein 24213753 136 LA_1053 24213780 aminopeptidase N 884 hypothetical protein 24213792 160 LA_1092 hypothetical protein 24213799 477 LA_1099 succinyl-CoA synthetase 24213801 291 subunit alpha succinyl-CoA synthetase 24213802 390 subunit beta hypothetical protein 24213807 278 LA_1107 adenylosuccinate 24213810 425 synthase ATP 24213811 phosphoribosyltransferas 344 e regulatory subunit glycerol-3-phosphate 24213812 669 dehydrogenase 24213819 dUTP diphosphatase 145 hypothetical protein 24213832 80 LA_1132 outer membrane 24213836 lipoprotein-sorting 257 protein hypothetical protein 24213837 371 LA_1137 2-methylthioadenine 24213838 437 synthetase anthranilate 24213840 phosphoribosyl 336 transferase pyrimidine deaminase, 24213844 466 riboflavin biosynthesis bifunctional 3,4- dihydroxy-2-butanone 4- 24213847 402 phosphate synthase/GTP cyclohydrolase II sulfate ABC transporter 24213855 335 substrate-binding protein 24213873 acetyl-CoA synthetase 660 ABC transporter 24213874 330 substrate-binding protein histidinol-phosphate 24213881 370 aminotransferase signal transduction 24213885 569 protein hypothetical protein 24213890 313 LA_1190 3-hydroxybutyryl-CoA 24213898 257 dehydratase thioesterase superfamily 24213907 140 protein dihydrolipoamide 24213922 419 acetyltransferase dihydrolipoamide 24213923 467 dehydrogenase alpha-ketoglutarate 24213924 920 decarboxylase threonyl-tRNA 24213940 639 synthetase translation initiation 24213942 179 factor IF-3 50S ribosomal protein 24213943 67 L35 50S ribosomal protein 24213944 117 L20 hypothetical protein 24213945 213 LA_1245 5-formyltetrahydrofolate 24213947 176 cyclo-ligase chemotaxis protein 24213951 1070 histidine kinase CheA chemotaxis-specific 24213952 357 methylesterase CheB two-component 24213953 response regulator 120 receiver protein segregation and 24213955 187 condensation protein B 3-phosphoshikimate 1- 24213958 440 carboxyvinyltransferase fatty acid/phospholipid 24213964 335 synthesis protein aspartate/tyrosine/arom 24213993 366 atic aminotransferase 24214013 glutamine synthetase 473 phosphoribosylformylgly 24214023 cinamidine (FGAM) 745 synthase isoleucyl-tRNA 24214025 914 synthetase hypothetical protein 24214029 239 LA_1329 capsule biosynthesis 24214051 342 protein CapA hypothetical protein 24214059 150 LA_1359 24214078 GTP-binding protein BipA 606 24214088 glycyl-tRNA synthetase 464 ribosomal protein L11 24214091 300 methylase 24214092 ribokinase 328 hypothetical protein 24214098 218 LA_1398 serine 24214109 hydroxymethyltransferas 415 e 24214117 lactoylglutathione lyase 152 hypothetical protein 24214120 125 LA_1420 24214122 serine/threonine kinase 1780 24214137 glucose kinase 298 carboxy-terminal 24214149 462 processing protease UDP-glucose 6- 24214159 436 dehydrogenase PLP dependent enzyme 24214180 222 class III 24214188 glycolate oxidase 760 hypothetical protein 24214195 331 LA_1495 24214211 L-aspartate oxidase 532 pyridoxal phosphate 24214214
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    Appendix 3 : Conserved proteins present in L. interrogans serovar Canicola L. interrogans serovar Canicola Accession Number Protein identification Protein Length (Amino Acids) Mean No. of peptides Mean % Coverage 5,10 methylene tetrahydrofolate 45655587 310 7.67 11.33 reductase 45655588 274 hypothetical protein LIC20005 4.33 20.00 45655592 547 porphobilinogen deaminase 4.33 7.67 delta-aminolevulinic acid 45655593 317 15.00 20.33 dehydratase glutamate-1-semialdehyde 45655594 443 24.00 24.33 aminotransferase 45655597 340 uroporphyrinogen decarboxylase 2.67 5.67 45655598 443 coproporphyrinogen III oxidase 5.00 12.33 45655605 200 azoreductase 5.33 18.00 45655609 251 short-chain dehydrogenase 2.33 14.33 45655611 422 NADH dehydrogenase 3.67 11.00 45655613 202 hypothetical protein LIC20030 2.33 15.67 45655615 140 hypothetical protein LIC20032 4.33 15.67 45655617 356 hypothetical protein LIC20034 2.33 8.33 45655618 440 hypothetical protein LIC20035 8.17 14.67 methanol dehydrogenase 45655620 357 7.83 19.17 regulator 45655627 607 heat shock protein 90 9.33 14.83 45655628 189 hypothetical protein LIC20045 2.33 8.33 45655641 670 methylmalonyl-CoA mutase 14.33 14.33 phosphoribosyl-ATP 45655645 92 10.67 13.00 pyrophosphatase 3-oxoacyl-(acyl-carrier protein) 45655647 254 4.00 17.00 reductase 45655648 77 acyl carrier protein 3.67 9.00 45655661 171 hypothetical protein LIC20078 5.00 26.00 4-hydroxybenzoyl-CoA 45655663 142 3.33 11.67 thioesterase S-adenosyl-L-homocysteine 45655666 436 13.00 19.67 hydrolase B12-dependent methionine 45655668 1247 42.67 21.67
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