Product Sheet Info

Product Information Sheet for NR-19703

Vibrio cholerae Gateway® Clone Set, Growth Conditions: Media: Recombinant in Escherichia coli, Plate 25 LB broth or agar containing 50 µg/mL kanamycin

Incubation: Catalog No. NR-19703 Temperature: E. coli, strain DH10B-T1 clones should be This reagent is the tangible property of the U.S. Government. grown at 37°C. Atmosphere: Aerobic For research use only. Not for human use. Propagation: 1. Scrape top of frozen well with a pipette tip and streak onto Contributor: agar plate. Pathogen Functional Genomics Resource Center at the J. 2. Incubate the plates at 37°C for 1 day. Craig Venter Institute Citation: Manufacturer: Acknowledgment for publications should read “The following BEI Resources reagent was obtained through BEI Resources, NIAID, NIH: ® Vibrio cholerae Gateway Clone Set, Recombinant in Product Description: Escherichia coli, Plate 25, NR-19703.”

Production in the 96-well format has increased risk of cross- contamination between adjacent wells. Individual clones Biosafety Level: 1 should be purified (e.g. single colony isolation and purification Appropriate safety procedures should always be used with this using good microbiological practices) and sequence-verified material. Laboratory safety is discussed in the following prior to use. BEI Resources does not confirm or validate publication: U.S. Department of Health and Human Services, individual mutants provided by the contributor. Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in The Vibrio cholerae (V. cholerae) Gateway® clone set consists Microbiological and Biomedical Laboratories. 5th ed. of 46 plates which contain 3813 sequence validated clones Washington, DC: U.S. Government Printing Office, 2007; see from V. cholerae, strain El Tor N16961 cloned in Escherichia www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm. coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR221 with a native start codon Disclaimers: and stop codon. The library was independently cloned and You are authorized to use this product for research use only. sequence verified by the Harvard Institute of Proteomics. It is not intended for human use. Detailed information about each clone is shown in Table 1. Use of this product is subject to the terms and conditions of the Information related to the use of Gateway® Clones can be BEI Resources Material Transfer Agreement (MTA). The MTA obtained from Invitrogen. Recombination was facilitated is available on our Web site at www.beiresources.org. through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR221) While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, to create an attL-containing entry clone. The entry clone ® contains recombinational cloning sites, attL1 and attL2 to neither ATCC nor the U.S. Government make any warranties facilitate gene transfer into a destination vector, M13 forward or representations as to its accuracy. Citations from scientific and reverse priming sites for sequencing and a kanamycin literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that resistance gene for selection. Please refer to the Invitrogen such information has been confirmed to be accurate. Gateway® Technology Manual for additional details.

This product is sent with the condition that you are responsible Material Provided: for its safe storage, handling, use and disposal. ATCC® and the Each inoculated well of the 96-well plate contains U.S. Government are not liable for any damages or injuries approximately 60 µL of E. coli culture (strain DH10B-T1) in arising from receipt and/or use of this product. While Luria Bertani (LB) broth containing 50 µg/mL kanamycin reasonable effort is made to ensure authenticity and reliability supplemented with 15% glycerol. of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for Packaging/Storage: damages arising from the misidentification or NR-19703 was packaged aseptically in a 96-well plate. The misrepresentation of products. product is provided frozen and should be stored at -80°C or colder immediately upon arrival. For long-term storage, the Use Restrictions: vapor phase of a liquid nitrogen freezer is recommended. This material is distributed for internal research, non- Freeze-thaw cycles should be avoided. commercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals

BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898

Product Information Sheet for NR-19703

contemplating commercial use of the material, its products or References: its derivatives must contact the contributor to determine if a 1. Heidelberg, J. F., et al. “DNA Sequence of both license is required. U.S. Government contractors may need a Chromosomes of the Cholera Pathogen Vibrio cholerae.” license before first commercial sale. Nature 406 (2000): 477-483. PubMed: 10952301

ATCC® is a trademark of the American Type Culture Collection.

Table 1: Vibrio cholerae Gateway® Clones, Plate 25 Clone Well ORF Locus ID Symbol Product Accession ID Position Length Number 174494 A02 118 VC2574 rpsM ribosomal protein S13 NP_232202.1 197988 A03 N/A VCA0131 rbsK ribokinase N/A 174749 A04 289 VC1165 conserved hypothetical protein NP_230810.1 174878 A05 135 VC0168 cycB cytochrome c5 NP_229825.1 174386 A06 635 VC0855 dnaK dnaK protein NP_230502.1 175488 A07 259 VC1224 hypothetical protein NP_230869.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N- 174653 B01 343 VC2329 dapD NP_231960.1 succinyltransferase 174499 B02 123 VC2586 rplN ribosomal protein L14 NP_232214.1 197991 B03 N/A VCA0619 hypothetical protein N/A 174757 B04 290 VC1177 conserved hypothetical protein NP_230822.1 spermidine-putrescine ABC transporter, periplasmic 174882 B05 359 VC0704 NP_230353.1 spermidine-putrescine-binding protein, putative 174394 B06 N/A VCA0287 thrS threonyl-tRNA synthetase N/A 175512 B07 293 VC0771 vibB vibriobactin-specific isochorismatase NP_230420.1 174282 B08 278 VC0638 folP dihydropteroate synthase NP_230287.1 174657 C01 894 VC1258 gyrA DNA gyrase, subunit A NP_230903.1 174503 C02 525 VC0680 mviN MviN protein NP_230329.1 cysteine synthase-cystathionine beta-synthase family 198012 C03 355 VC1061 NP_230706.1 protein 174765 C04 298 VC0161 ilvY transcriptional activator IlvY NP_229818.1 174906 C05 391 VC0705 pheA chorismate mutase-prephenate dehydratase NP_230354.1 176113 C06 N/A VCA0820 groEL-2 chaperonin, 60 Kd subunit N/A 175536 C07 305 VC0764 hypothetical protein NP_230413.1 200919 C08 649 VC0298 acs-1 acetyl-CoA synthase NP_229953.2 174673 D01 903 VC2394 secA preprotein translocase, SecA subunit NP_232024.1 174507 D02 128 VC2570 rplQ ribosomal protein L17 NP_232198.1 198026 D03 405 VC1727 glgC-1 glucose-1-phosphate adenylyltransferase NP_231363.1 174781 D04 304 VC0718 conserved hypothetical protein NP_230367.1 175216 D05 323 VC2385 RNA-directed DNA polymerase NP_232015.1 176121 D06 548 VC0725 pstA-1 phosphate ABC transporter, permease protein NP_230374.1 175728 D07 489 VC0767 guaB inosine-5`-monophosphate dehydrogenase NP_230416.1 200920 D08 979 VC0812 helicase-related protein NP_230461.1 UDP-N-acetylglucosamine--N-acetylmuramyl- 174677 E01 354 VC2401 murG (pentapeptide) pyrophosphoryl-undecaprenol N- NP_232031.1 acetylglucosamine transferase 174511 E02 544 VC0085 ubiquinone biosynthesis protein AarF, putative NP_229744.1 198036 E03 N/A VCA0605 aminotransferase, class III N/A 174799 E04 65 VC0713 hypothetical protein NP_230362.1 175220 E05 129 VC1895 conserved hypothetical protein NP_231529.1 176135 E06 644 VC1872 conserved hypothetical protein NP_231506.1 175743 E07 517 VC0768 guaA GMP synthase NP_230417.1 175647 E08 N/A VCA0732 conserved hypothetical protein N/A 174693 F01 338 VC2623 trpS tryptophanyl-tRNA synthetase NP_232251.2

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Product Information Sheet for NR-19703

Clone Well ORF Locus ID Symbol Product Accession ID Position Length Number 198038 F03 N/A VCA0137 glpT glycerol-3-phosphate transporter N/A 174811 F04 321 VC1179 pseudouridine synthase family 1 protein NP_230824.1 vibriobactin and enterobactin ABC transporter, 175224 F05 325 VC0776 viuP periplasmic ferric vibriobactin-enterobactin-binding NP_230425.1 protein 175104 F06 276 VC2381 conserved hypothetical protein NP_232011.1 175765 F07 556 VC0734 aceB malate synthase A NP_230383.1 175698 F08 446 VC0766 xseA exodeoxyribonuclease VII, large subunit NP_230415.1 174709 G01 367 VC2289 apbE thiamin biosynthesis lipoprotein ApbE NP_231920.1 174539 G02 146 VC2730 epsG general secretion pathway protein G NP_232357.1 198044 G03 532 VC2237 mltD membrane-bound lytic murein transglycosylase D NP_231868.1 174819 G04 323 VC1163 conserved hypothetical protein NP_230808.1 175112 G06 279 VC1900 fadR fatty acid metabolism regulator protein NP_231534.1 174180 G07 419 VC2180 hemA glutamyl-tRNA reductase NP_231712.1 174721 H01 1375 VC0328 rpoB DNA-directed RNA polymerase, beta subunit NP_229982.1 174543 H02 600 VC2041 conserved hypothetical protein NP_231675.1 198046 H03 N/A VCA0601 ABC transporter, permease protein N/A phosphate ABC transporter, periplasmic phosphate- 174843 H04 325 VC0721 NP_230370.1 binding protein, putative 174338 H05 334 VC2266 thiL thiamin-monophosphate kinase NP_231897.1 vibriobactin and enterobactin ABC transporter, ATP- 175120 H06 279 VC0779 viuC NP_230428.1 binding protein 174228 H07 439 VC0233 kdtA 3-deoxy-D-manno-octulosonic-acid transferase NP_229890.1 175361 H08 224 VC1232 conserved hypothetical protein NP_230877.1

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