Product datasheet Anti-MED6 Antibody Catalog Number: A09224

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-MED6 Antibody Name MED6 Source Rabbit IgG Species Reactivity ,mouse,rat Tested Application WB,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E. coli-derived human MED6 recombinant protein (Position: F40-Q246). Purification Immunogen affinity purified. Observed MW 28KD Dilution Ratios Western blot: 1:500-2000 Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an that in is encoded by the MED6 gene. This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-MED6 Antibody Catalog Number: A09224

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot analysis of MED6 using anti-MED6 antibody (A09224). Lane 1: human A431 whole cell lysates,Lane 2: human U2OS whole cell lysates. anti-MED6 antigen affinity purified polyclonal antibody (Catalog # A09224)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for MED6 at approximately 32KD. The expected band size for MED6 is at 28KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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