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Scientific Tracks & Abstracts conferenceseries.com conferenceseries.com 963rd Conference 10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan Posters Scientific Tracks & Abstracts Page 49 Yu-Kuo Chen et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045 10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan Pharmacokinetic studies of pterostilbene and 3'-hydroxypterostilbene in rats Yu-Kuo Chen1, Hui-Yun Tsai2 and Chi-Tang Ho2 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA ur previous results demonstrated that 3'-hydroxypterostilbene (OHPt) has higher anti-tumor activity than pterostilbene (Pt) Oin vivo. In the present study, we would like to elucidate whether the stronger biological activity of OHPt is due to its higher bioavailability than Pt. We performed pharmacokinetic studies in male Sprague Dawley rats. The animals received a single oral administration of OHPt and Pt at the dose of 50 mg/kg through oral gavage. After oral administration, a series of plasma samples were collected at 0, 5, 10, 15, 30, 60, 120, 180, 240, 480 and 600 min for further HPLC analysis. The results showed that two unidentified metabolites without parent compound OHPt were observed following ingestion of OHPt; while only one metabolite (glucuronide/ sulfate conjugates) and parent compound Pt were detected in rat plasma after ingestion of Pt. These results indicated that the biotransformation of OHPt might not be glucuronide or sulfate conjugation, whereas, the major biotransformation pathway of Pt might be glucuronide or sulfate conjugation. The findings of this study provide message that despite structural similarity, Pt and its hydroxyl analog OHPt exert completely differential effects on oral bioavailability and metabolism. Further experiment is needed to identify the unknown metabolites of OHPt. Biography Yu-Kuo Chen has received his PhD from the Institute of Food Science and Technology of National Taiwan University in 2009. After completing around two years as a Post-doctoral Associate in the Department of Chemical Biology at Rutgers University, he joined the faculty at National Pingtung University of Science and Technology as an Assistant Professor in the Department of Food Science. His research focuses on the biological evaluation of functional foods, purification and identification of active components in functional foods and analysis of metabolism and bioavailability of those compounds. [email protected] Notes: J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017 Page 50 Yi-Zhen Chen et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045 10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan Regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters Yi-Zhen Chen, Jia-Lin Guo, Pi-Jen Tsai, Mei-Li Wu and Yu-Kuo Chen National Pingtung University of Science and Technology, Taiwan eart disease and cerebrovascular disease are the second and third leading cause of death in Taiwan in 2015. Many studies showed Hthat the most important factor leading to cardiovascular-related diseases is atherosclerosis, and the main caused by atherosclerosis is high blood cholesterol levels. Over the years, the risk of cardiovascular disease associated with population continues to increase, so the healthy diet and exercise have become one of the important issues. Djulis (Chenopodium formosanum) is a native cereal plant in Taiwan, and the Djulis Hull (DH) was found to be its major source of antioxidant compound. Studies showed that it possesses several active components with hypolipidemic activities, such as dietary fiber and polyphenol compounds. The objective of this investigation was to evaluate the regulation effects of DH in blood lipid levels to achieve the prevention of atherosclerosis effect. Five weeks old hamsters were randomly divided into five groups: (1) control group, (2) hyperlipidemic group (high fat diet containing 0.2% cholesterol; HFC), (3) low-dose DH group (HFC diet and treated with 100 mg/kg/day DH; LDH), medium-dose DH group (HFC diet and treated with 250 mg/kg/day DH; MDH), high-dose DH group (HFC diet and treated with 500 mg/kg/day DH; HDH). After 8 weeks of feeding study, the results showed that levels of triglyceride (TG; LDH: 194±31 mg/dl; MDH: 194±25 mg/dl; HDH: 204±26 mg/dl), total cholesterol (TC; LDH: 328±25 mg/dl; MDH: 301±21 mg/dl; HDH: 283±41 mg/dl) and low-density lipoprotein cholesterol (LDL-C; LDH: 165±33 mg/dl; MDH: 145±31 mg/dl; HDH: 140±28 mg/dl) in serum were significantly reduced by DH administration compared with those of HFC group (TG: 309±64 mg/dl; TC: 398±79 mg/dl; LDL-C: 184±31 mg/dl). Moreover, levels of TG and TC in liver were lower than HFC group, and levels of TG and TC in feces were higher than HFC group. These results indicate that DH possesses hypolipidemic potential to be a benefit functional food to prevent high fat/high cholesterol diet-induced hyperlipidemia. Biography Yi-Zhen Chen is currently a Master’s student in the Department of Food Science at the University of National Pingtung University of Science and Technology. She has obtained her Bachelor's degree in Food Science at NPUST in June 2015. She has full of great interests in the knowledge about Food Science. Her Master’s thesis title is “The regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters”. She has published one peer-reviewed research article in SCI international journal and five conference posters and has passed the required qualification examination for six technician certificates. [email protected] Notes: J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017 Page 51 Jia-Lin Guo et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045 10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan Study on bioavailability of 5-demethyl tangeretin and its acetylated derivative in PC-3 cells by HPLC with electrochemical detector Jia-Lin Guo1, Yin-Bo Chen1, Hui-Yun Tsai2, Shiming Li2, Chi-Tang Ho2 and Yu-Kuo Chen1 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA angeretin (TAN), a kind of polymethoxyflavones (PMFs) is found in citrus peels and has been proved to have many kinds of Tbioactivities including anti-inflammatory, anti-atherosclerosis and antitumor activities. Prostate cancer is universal malignant in male genital system. According to studies, 5-demethyltangeretin has hydroxyl group at the C5 position, and higher cytotoxicity than TAN for cancer cells, but the low solubility of 5-DTAN limits their availability in biological system. For the reason that we prepared 5-DTAN derivative (with acetyl group at the C5 position, 5-acetyltangeretin derivative; 5-ATAN), and hope to increase its solubility and bioavailability. The objective of this study is to investigate the anti-proliferation and cellular uptake of 5-ATAN in PC-3 human prostate cancer cells. Esterase present in the cells, therefor, 5-ATAN will be converted to 5-DTAN when 5-ATAN was uptaken into cells. We could use high-performance liquid chromatography lined electrochemical detector (ECD) to detect concentration of 5-DTAN within PC-3 cell. In MTT assay, it demonstrated that 5-ATAN significantly inhibit growth of PC-3 cells in dose- and time-depend manners. In condition of HPLC-ECD showed that 300 mV is the best volt, and retention time of 5-DTAN is 11.6 minutes, and linear regression is 0.1-10 µM. Moreover, the quantification and detection limits (LOQ and LOQ) of 5-DTAN were 7.16 and 1.79 μg/ml, respectively. In bioavailability test, intracellular concentration of 5-DTAN of 5-ATAN-treated group was significantly higher than those of 5-DTAN-treated group, which showed dose- and time-depend responses. Overall, the results indicate that 5-ATAN showed highest anti-proliferation activity than TAN & 5-DTAN in PC-3 cancer cells, and had greater bioavailability than 5-DTAN. 5-ATAN will be expected to have better oral bioavailability in vivo. Biography Jia Lin Guo is currently pursuing his Master’s degree in Food Science from National Pingtung University of Science and Technology and has expertise in HPLC analysis of natural compounds. [email protected] Notes: J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017 Page 52 Po-Hua Wu et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045 10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan Regulation effect of tempe (tempeh) in serum glucose in type II diabetic rats Po-Hua Wu, Ming-Chang Wu and Yu-Kuo Chen National Pingtung University of Science and Technology, Taiwan n recent years, tempe (tempeh) is being more attention than before. Tempe (tempeh) originates from traditional food of Indonesia, Iis produced by fermentation of soybean. Traditional tempeh only uses Rhizopus to ferment, trying Lactobacillus co-culture with Rhizopus to create more comfortable condition for fermentation. Diabetes is an increasingly serious disease of modern civilization. A healthy body secretes insulin to regulate blood glucose by pancreas. Through the action of insulin so that glucose in the blood transport into cells to provide energy which cells need. When the level of insulin is not enough or inefficient use of insulin to reduce blood sugar in body, the problem of high blood sugar will occur.
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