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conferenceseries.com 963rd Conference
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Posters Scientific Tracks & Abstracts
Page 49 Yu-Kuo Chen et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Pharmacokinetic studies of pterostilbene and 3'-hydroxypterostilbene in rats Yu-Kuo Chen1, Hui-Yun Tsai2 and Chi-Tang Ho2 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA
ur previous results demonstrated that 3'-hydroxypterostilbene (OHPt) has higher anti-tumor activity than pterostilbene (Pt) Oin vivo. In the present study, we would like to elucidate whether the stronger biological activity of OHPt is due to its higher bioavailability than Pt. We performed pharmacokinetic studies in male Sprague Dawley rats. The animals received a single oral administration of OHPt and Pt at the dose of 50 mg/kg through oral gavage. After oral administration, a series of plasma samples were collected at 0, 5, 10, 15, 30, 60, 120, 180, 240, 480 and 600 min for further HPLC analysis. The results showed that two unidentified metabolites without parent compound OHPt were observed following ingestion of OHPt; while only one metabolite (glucuronide/ sulfate conjugates) and parent compound Pt were detected in rat plasma after ingestion of Pt. These results indicated that the biotransformation of OHPt might not be glucuronide or sulfate conjugation, whereas, the major biotransformation pathway of Pt might be glucuronide or sulfate conjugation. The findings of this study provide message that despite structural similarity, Pt and its hydroxyl analog OHPt exert completely differential effects on oral bioavailability and metabolism. Further experiment is needed to identify the unknown metabolites of OHPt.
Biography Yu-Kuo Chen has received his PhD from the Institute of Food Science and Technology of National Taiwan University in 2009. After completing around two years as a Post-doctoral Associate in the Department of Chemical Biology at Rutgers University, he joined the faculty at National Pingtung University of Science and Technology as an Assistant Professor in the Department of Food Science. His research focuses on the biological evaluation of functional foods, purification and identification of active components in functional foods and analysis of metabolism and bioavailability of those compounds.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 50 Yi-Zhen Chen et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters Yi-Zhen Chen, Jia-Lin Guo, Pi-Jen Tsai, Mei-Li Wu and Yu-Kuo Chen National Pingtung University of Science and Technology, Taiwan
eart disease and cerebrovascular disease are the second and third leading cause of death in Taiwan in 2015. Many studies showed Hthat the most important factor leading to cardiovascular-related diseases is atherosclerosis, and the main caused by atherosclerosis is high blood cholesterol levels. Over the years, the risk of cardiovascular disease associated with population continues to increase, so the healthy diet and exercise have become one of the important issues. Djulis (Chenopodium formosanum) is a native cereal plant in Taiwan, and the Djulis Hull (DH) was found to be its major source of antioxidant compound. Studies showed that it possesses several active components with hypolipidemic activities, such as dietary fiber and polyphenol compounds. The objective of this investigation was to evaluate the regulation effects of DH in blood lipid levels to achieve the prevention of atherosclerosis effect. Five weeks old hamsters were randomly divided into five groups: (1) control group, (2) hyperlipidemic group (high fat diet containing 0.2% cholesterol; HFC), (3) low-dose DH group (HFC diet and treated with 100 mg/kg/day DH; LDH), medium-dose DH group (HFC diet and treated with 250 mg/kg/day DH; MDH), high-dose DH group (HFC diet and treated with 500 mg/kg/day DH; HDH). After 8 weeks of feeding study, the results showed that levels of triglyceride (TG; LDH: 194±31 mg/dl; MDH: 194±25 mg/dl; HDH: 204±26 mg/dl), total cholesterol (TC; LDH: 328±25 mg/dl; MDH: 301±21 mg/dl; HDH: 283±41 mg/dl) and low-density lipoprotein cholesterol (LDL-C; LDH: 165±33 mg/dl; MDH: 145±31 mg/dl; HDH: 140±28 mg/dl) in serum were significantly reduced by DH administration compared with those of HFC group (TG: 309±64 mg/dl; TC: 398±79 mg/dl; LDL-C: 184±31 mg/dl). Moreover, levels of TG and TC in liver were lower than HFC group, and levels of TG and TC in feces were higher than HFC group. These results indicate that DH possesses hypolipidemic potential to be a benefit functional food to prevent high fat/high cholesterol diet-induced hyperlipidemia.
Biography Yi-Zhen Chen is currently a Master’s student in the Department of Food Science at the University of National Pingtung University of Science and Technology. She has obtained her Bachelor's degree in Food Science at NPUST in June 2015. She has full of great interests in the knowledge about Food Science. Her Master’s thesis title is “The regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters”. She has published one peer-reviewed research article in SCI international journal and five conference posters and has passed the required qualification examination for six technician certificates.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 51 Jia-Lin Guo et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Study on bioavailability of 5-demethyl tangeretin and its acetylated derivative in PC-3 cells by HPLC with electrochemical detector Jia-Lin Guo1, Yin-Bo Chen1, Hui-Yun Tsai2, Shiming Li2, Chi-Tang Ho2 and Yu-Kuo Chen1 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA
angeretin (TAN), a kind of polymethoxyflavones (PMFs) is found in citrus peels and has been proved to have many kinds of Tbioactivities including anti-inflammatory, anti-atherosclerosis and antitumor activities. Prostate cancer is universal malignant in male genital system. According to studies, 5-demethyltangeretin has hydroxyl group at the C5 position, and higher cytotoxicity than TAN for cancer cells, but the low solubility of 5-DTAN limits their availability in biological system. For the reason that we prepared 5-DTAN derivative (with acetyl group at the C5 position, 5-acetyltangeretin derivative; 5-ATAN), and hope to increase its solubility and bioavailability. The objective of this study is to investigate the anti-proliferation and cellular uptake of 5-ATAN in PC-3 human prostate cancer cells. Esterase present in the cells, therefor, 5-ATAN will be converted to 5-DTAN when 5-ATAN was uptaken into cells. We could use high-performance liquid chromatography lined electrochemical detector (ECD) to detect concentration of 5-DTAN within PC-3 cell. In MTT assay, it demonstrated that 5-ATAN significantly inhibit growth of PC-3 cells in dose- and time-depend manners. In condition of HPLC-ECD showed that 300 mV is the best volt, and retention time of 5-DTAN is 11.6 minutes, and linear regression is 0.1-10 µM. Moreover, the quantification and detection limits (LOQ and LOQ) of 5-DTAN were 7.16 and 1.79 μg/ml, respectively. In bioavailability test, intracellular concentration of 5-DTAN of 5-ATAN-treated group was significantly higher than those of 5-DTAN-treated group, which showed dose- and time-depend responses. Overall, the results indicate that 5-ATAN showed highest anti-proliferation activity than TAN & 5-DTAN in PC-3 cancer cells, and had greater bioavailability than 5-DTAN. 5-ATAN will be expected to have better oral bioavailability in vivo.
Biography Jia Lin Guo is currently pursuing his Master’s degree in Food Science from National Pingtung University of Science and Technology and has expertise in HPLC analysis of natural compounds.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 52 Po-Hua Wu et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Regulation effect of tempe (tempeh) in serum glucose in type II diabetic rats Po-Hua Wu, Ming-Chang Wu and Yu-Kuo Chen National Pingtung University of Science and Technology, Taiwan
n recent years, tempe (tempeh) is being more attention than before. Tempe (tempeh) originates from traditional food of Indonesia, Iis produced by fermentation of soybean. Traditional tempeh only uses Rhizopus to ferment, trying Lactobacillus co-culture with Rhizopus to create more comfortable condition for fermentation. Diabetes is an increasingly serious disease of modern civilization. A healthy body secretes insulin to regulate blood glucose by pancreas. Through the action of insulin so that glucose in the blood transport into cells to provide energy which cells need. When the level of insulin is not enough or inefficient use of insulin to reduce blood sugar in body, the problem of high blood sugar will occur. The most common diabetes is type II, which is acquired diabetes. It is confirmed that aglycone can help regulation of blood sugar. Tempe has more aglycone than other soybeans product. This study observes changes of blood sugar in diabetic rats by fed tempe. To induce diabetes uses pharmacy with high-fat diet. Using streptozotocin (STZ) with nicotinamide induces rats to get diabetes and make sure their blood sugar above 150 (mg/dl). At same time, rats had fed high fat diet which includes cholesterol, coconut oil and normal diet. Comparing tempe, tempe with Lactobacillus are useful with negative control and soybeans groups. Obviously, diabetic rats’ regulation of blood sugar with tempe are very useful.
Biography Po-Hua Wu is a second year Master’s student at Department of Food Science in National Pingtung University of Science and Technology.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 53 Chien-Hua Huang et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Study on the protective effects of tocotrienols in SH-SY5Y cells Chien-Hua Huang1, Yun-Xuan Zhang2, Yi-Lin Zheng2, Chung S Yang2 and Yu-Kuo Chen1 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA
ocotrienols and tocopherols, naturally occurring isoforms of vitamin E, are found abundantly in food such as palm oil, rice bran oil, Tcorn, oats, barley, rye and wheat. Tocotrienols have four different isomers: α-, β-, γ- and δ-forms. The present study was designed to evaluate the protective effect of tocotrienols against methylglyoxal (MG)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. In order to assess the protective effect of tocotrienols, α-tocotrienol (α-T3), γ-tocotrienol (γ-T3) and δ-tocotrienol (δ-T3) were administered to SH-SY5Y cells for 2 hours prior to the induction of cytotoxicity by MG, and the cell viabilities of SH-SY5Y cells were analyzed by MTT assay. The cell viability of SH-SY5Y cells were reduced to 40% by 24 h treatment of 0.5 mM MG, and two hours pretreatment of α-T3, γ-T3 and δ-T3 increased the cell viability to 75%, 53% and 82%, respectively. It is shown that T3 has protective effect on the neuro-toxicity induction by MG. Among T3, δ-T3 has the best protective activity. The future work needs to be performed to elucidate the neuro-protective mechanism of T3.
Biography Chien-Hua Huang is currently pursuing her Master’s degree in the Food Science Department at National Pingtung University of Science and Technology. She has a great passion for food science and has the expertise in cell and animal experiments and biochemical techniques such as Western blotting. She is studying the protective effect of Corbicula fluminea extracts in RGM-1 cell against indomethacin-induced gastric mucosal injury. She has published 4 posters, one of which was presented at the ISNFF International Conference in 2016.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 54 Ching-Yi Kuan et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Study on the inhibitory activity of 3'-hydroxypterostilbene on tumor growth in xenograft model of human prostate cancer cells Ching-Yi Kuan1, Hui-Yun Tsai2, Chi-Tang Ho2 and Yu-Kuo Chen1 1National Pingtung University of Science and Technology, Taiwan 2Rutgers University, USA
ccording to the Ministry of Health and Welfare in Taiwan, prostate cancer is the sixth highest cause of cancer-related death. ABesides, prostate cancer cells will metastasize to bones and lymph nodes, so it’s important that prevention and treatment of prostate cancer. In this study, 3'-hydroxypterostilbene (OHPt), a structural analogue of pterostilbene (Pt), were used as experimental samples to investigate the potent anti-tumor effects in PC-3 xenografted nude mice. 1×106 PC-3 cells were injected subcutaneously into the flanks of 30 male Balb/c nude mice to form tumors. When xenografted tumor volume reached around 100 mm3, mice were randomly divided into five groups. Each group consisted of 6 animals. Mice were orally administrated with daily doses of OHPt (5, 25 and 50 mg/kg/d) and Pt (25 mg/kg/d) for 4 weeks; while control group was orally administrated with olive oil only. During the experiment, the tumor volume was determined and recorded once or twice per week using caliper measurements. At the end of the experiment, animals were
sacrificed by CO2 asphyxiation and the individual tumors were excised immediately and weighed. Further the protein expression of tumors was measured. The results showed that the treatment of mice with OHPt could inhibit the tumor volume and tumor weight in a dose-dependent manner, and increased the expression of c-caspase-3 while decreased the expression of MMP-9 and Cox-2. In conclusion, these results revealed that OHPt might be a potential agent to treat prostate cancer.
Biography Ching-Yi Kuan is currently a Master’s student in the Department of Food Science at National Pingtung University of Science and Technology in Taiwan. She has a great passion for Food Science. She can perform cell experiments, western blotting and animal experiments. She is studying the inhibitory activity of the stilbenes on liver cancer. She has published one poster in Taiwan Association for Food and Technology in 2015.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 55 Taejoon Kang et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
A novel and highly specific phage endolysin cell wall binding domain for detection ofBacillus cereus Taejoon Kang, Gayoung Eom, Ahreum Hwang and Jeong Moon KRIBB, Korea
apid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety.Bacillus cereus is harmful as Rit causes foodborne illness and a number of systemic and local infections. We report a novel phage endolysin cell wall-binding domain (CBD) for B. cereus and the development of a highly specific and sensitive surface plasmon resonance (SPR)-based B. cereus detection method using the CBD. The newly discovered CBD from endolysin of PBC1, aB. cereus-specific bacteriophage, provides high specificity and binding capacity to B. cereus. By using the CBD-modified SPR chips, B. cereus can be detected at the range of 105–108 CFU/ml. More importantly, the detection limit can be improved to 102 CFU/ml by using a subtractive inhibition assay based on the pre-incubation of B. cereus and CBDs, removal of CBD-bound B. cereus, and SPR detection of the unbound CBDs. The present study suggests that the small and genetically engineered CBDs can be promising biological probes for B. cereus. We anticipate that the CBD based SPR-sensing methods will be useful for the sensitive, selective, and rapid detection of B. cereus.
Biography Taejoon Kang received a BS degree in Chemistry from KAIST in 2004 and a PhD in Chemistry from KAIST in 2010. He did his Post-doctoral research at KAIST before joining in KRIBB as a Senior Researcher in 2012. He is currently working as Senior Researcher at BioNanotechnology Research Center and BioNano Health Guard Research Center in KRIBB and Associate Professor in major of Nanobiotechnology and Bioinformatics at UST.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 56 Suh-Jen Jane Tsai et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
HPLC-fluorescence and LC-MS/MS detection of trans-resveratrol, quercetin and emodin in grape and red wine with SFE Suh-Jen Jane Tsai, Yu-Ren Lin, Cheng-Ru Tsai and An-Kai Syu Providence University, Taiwan
he identification and analysis of three anti-oxidants including: trans-resveratrol, quercetin and emodin in grape and red wine Thave been studied by HPLC-fluorescence and LC-MS/MS detection with SFE in this work. Trans-resveratrol (trans-3,5,4'- trihydroxystilbene, resveratrol) is commonly found in grape and red wine. Trans-resveratrol (trans-RSV) has important effects on lipid metabolism and can be used as anti-inflammatory, anti-oxidant, free radical scavenging, and anti-allergic agent. It can also prevent the cardiovascular diseases. Besides, trans-RSV is also an effective cancer preventive medication. It can effectively inhibit tumor growth and cancer formation. Trans-resveratrol is sensitive to light and heat. Consequently, trans-RSV will convert to inactive cis-RSV. The coexistence of antioxidants will also cause activity recession. In this work, quercetin (2-(3,4-dihydroxyphenyl)- 3,5,7-trihydroxy- 4H-chromen-4-one) and emodin (1,3,8-trihydroxy-6-methylanthracene- 9,10-dione were also studied for their interferences in the analytical procedure for trans-RSV. The sensitivity and recovery for the proposed method for natural trans- resveratrol from grape and red wine were also evaluated.
Biography Suh-Jen Jane Tsai is a Professor and an affiliate of department of applied chemistry in the Providence University of Taichung, Taiwan.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 57 Ponpis Raungkhajon et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Nutritional management in upper gastrointestinal tract cancers Ponpis Raungkhajon, Kanjana Chimrueng and Pakjira BenyaPanya Prince of Songklanagarind Hospital, Thailand
n a study of 224 patients, comprising 157 male patients and 57 female patients, to determine the correlations between Body Mass IIndex (BMI) and the risk of malnutrition, 124 patients were found to have BMI of 16.00-18.49 Kg/m2 while 54 patients were found to have BMI less than 16.00 Kg/m2. Using the same sample, an assessment of nutritional status of the patients using the Nutrition Alert Form shows that 78 patients had severe malnutrition while 112 patients had moderate malnutrition. In addition, by monitoring the change in patients’ weight over the course of 4 weeks, the study shows that 69 patients or 30% of the total sample experienced over 5% weight-loss, 36 patients or 16% experienced 2 - 5% weight-loss, and 108 patients or 48.2% experienced 1 - 2% weight-loss. 160 patients had low albumin serum with 143 patients having 2.0 - 3.5 mg% of albumin serum and 17 patients having less than 2.0 mg% of albumin serum. Patients received enteral nutrition support according to Nutrition Care Process for Cancer Treatment Plan with 115 patients or 51.3% of the total sample receiving 1.2 kcal/1kd/d and 15-20 gm protein, 66 patients or 29.4% receiving 1.25 kcal/1kd/d and 10 - 15 gm protein, 31 patients or 13.8% receiving 1.5 kcal/1kd/d, and 12 patients or 5.5% receiving 1 kcal/1kd/d and 20 - 40 gm protein. Our patient care team is a multidisciplinary patient care approach designed to implement a mixed and integrated patient care system. In particular, the pre-treatment nutritional care is central to the reduction of risk of malnutrition and allows quick and uninterrupted access to nutritional care through our care process.
Biography Ponpis Raungkhajon is a Senior Professional Level Nutrition Officer at Prince of Songklanagarin Hospital located in Songkla,Thailand.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 58 Pakjira BenyaPanya et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Developing soy and soy-based recipes for menopausal women Pakjira BenyaPanya, Kanjana Chimrueng and Ponpis Raungkhajon Prince of Songklanagarind Hospital, Thailand
Background: The Nutritional Unit of the Prince of Songkhla University Hospital aspired to develop recipes from soy and soy-based products, which are rich in phytoestrogen and may help improve health condition and prevent medical problems, as a healthcare alternative for patients and their family. Objectives: To study and develop recipes from soy and soy-based products for female patients of OBGYN Clinic at the Prince of Songkhla University Hospital who are in their menopause. Methodology: Developing 4 recipes from soy and soy-based products. Result: Southern Coconut-Rice with Chicken Curry provides the highest level of energy at 460 Kcal, followed by Stuffed Glutinous Rice Balls at 335 Kcal, Spicy Tofu at 270 Kcal, while Baby Mushroom provides the lowest level of energy at 190 Kcal. In addition, a look at how patients assess the recipes shows that the effectiveness of the recipe in controlling underlying medical conditions ranks highest with an average score of 4.43, followed by taste at 4.31, and presentation/appeal at 4.17. The assessment criteria with the lowest score were the ease of cooking, which only received 3.95 on average. The recipes were then assessed on 3 subsequent assessment criteria with the following results- Taste: Stuffed Glutinous Rice Balls scored highest at 4.6, followed by Spicy Tofu at 4.3, and Coconut-Rice with Chicken Curry at 4.25. Baby Mushroom scored lowest at 4.1; Presentation/Appeal: Spicy Tofu scored highest at 4.47, followed by Stuffed Glutinous Rice Balls at 4.2, while Coconut-Rice with Chicken Curry and Baby Mushroom received an equal score of 4 and; Ease of Cooking: Spicy Tofu score highest at 4.2, followed by Coconut-Rice with Chicken Curry at 4, while Baby Mushroom and Stuffed Glutinous Rice Balls received an equal score of 3.8.
Biography Pakjira BenyaPanya is a Senior Professional Level Nutrition Officer at Prince of Songklanagarin Hospital located in Songkla,Thailand.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 59 Ting-Yi Chien et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Neuroprotective effects of Paecilomyces hepiali mycelium extract in Drosophila models of Huntington's disease Ting-Yi Chien1, Pin-Chieh Liu1 and Po-An Yeh2 1Shih Chien University, Taiwan 2Academia Sinica, Taiwan
s the world aging rapidly, neurodegenerative diseases became one of the most serious age-related health problems. In south Asia, Aseveral medical foods have been used as an anti-aging remedy since ancient times. Cordyceps (Paecilomyces hepiali mycelium), a traditional tonic, has been reported with various pharmacological activities. In this study, we utilized Drosophila model system mimicking Huntington’s disease (HD) to exam the neuroprotective effects of the ethanol extraction of P. hepiali mycelium (PHE). These HD flies are expressing motor dysfunction and early death. Results demonstrated that diet containing 1 mg/mL PHE could significantly prolong the mean lifespan up to 31% of flies compared to the control diet. It was also found that PHE could improve the locomotor activity of flies at the young age (4 days). Besides, oxidative stress plays a causal role in the neurodegenerative process. PHE exhibit anti-oxidative properties in a concentration-dependent manner by using the DPPH radical scavenging and ferric ions reducing power assay. These results indicated that supplementation with PHE improved movement performance and longevity might contribute by its neuroprotective antioxidants. We suggest that the PHE can be developed as a potential dietary supplement against neurodegenerative diseases such as HD.
Biography Ting-Yi Chien is an Assistant Professor of Food Science, Nutrition, and Nutraceutical Biotechnology at Shih Chien University, Taiwan. His research field includes food analysis and bioassay with special focus on anti-cancer, anti-inflammatory, and analgesic effects by phytochemicals from food. Recently, he is developing the anti-aging model to elucidate the protective effects of medical food on degenerative diseases such as osteoarthritis, sarcopenia and neurodegeneration.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 60 Ji Yeon Kwak et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Regulation and current status on approval of novel food ingredients in Korea Ji Yeon Kwak, Mi Ran Jang, Su Eun Lee, Woo Young Lee, Myung-Sang Yoo, Ji-Eun Shin, Ji Sun Park, Yun-Sook Kang and Jin-Hwan Hong National Institute of Food & Drug Safety Evaluation, Korea
ovel food ingredient means any food ingredient that has no history of consumption in Korea. Foods derived from sources Nnot previously used as human foods must be evaluated for safety. Novel food ingredients scope is the agricultural, livestock, marine products and microorganisms newly introduced in Korea as well as the ingredients acquired from extraction, separation and cultivation of those materials. Since 2010, applicants are required under 'Temporary Standards and Regulations for food Ingredients (MFDS Notification No.2016-27)' to submit information to Ministry of Food and Drug Safety (MFDS) regarding the product (e.g., the status of approval and use in other countries, manufacturing methods, ingredient characteristics, toxicological test, evaluation of food intake, etc.) In March 2017, the record shows that there have been 20 cases that are approved as novel food ingredients including insects, plants, microorganisms, and sugars. Among the approved novel food ingredients, insects are Mealworm (Tenebrio molitor Linne.) larva, White worm (Protaetia brevitarsis) larva, Dynastid beetle (Allomyrina dichotoma) larva and Two-spotted cricket (Gryllus bimaculatus) and all sugars (6 cases) are allulose (liquid, powder) that is commercialized in Japan and the U.S.A. As a substance that exists in nature, allulose is found in small quantities figs and raisins, etc. Allulose has 70% relative sweetness to sucrose. The calorie of allulose is 0.2 kcal which is 5% lower than sucrose (4 kcal). Since 2016, Korea has continuously carried out national sugar intake reduction project and allulose will help reduce sugar intake as a low-calorie alternative sweetener.
Biography Ji Yeon Kwak is an affiliate of National Institute of Food & Drug Safety Evaluation at Ministry of Food and Drug Safety in South Korea.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 61 Chun-Jung Chen et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Nutritional strategy against Japanese encephalitis-associated neuro-inflammation Chun-Jung Chen, Su-Lan Liao, Shue-Ling Raung and Yu-Hui Hu Taichung Veterans General Hospital, Taiwan
Statement of the Problem: Neuroinflammation has a central role in common pathologies of brain diseases. Japanese encephalitis, which is caused by infection with mosquito-transmitted Japanese encephalitis virus (JEV), is an example of an immunopathological disease closely associated with pathological disruption of the BBB and severe neuroinflammation. Those immunopathological changes correlate well with the onset and disease progression of Japanese encephalitis and represent promising targets for therapeutic control. With a view to gaining a better understanding of the pathogenic process and developing therapeutic options for Japanese encephalitis, further investigations of the underlying neuroinflammatory mechanisms during the course of JEV infection are needed. DHA possesses nutritional and pharmaceutical properties with a broad range of applications, including anti-inflammation. Since dietary and nutraceutical supplements of DHA are increasingly becoming acceptable options for health promotion and/or therapeutic treatments, the purpose of this study was to investigate the effect of DHA on Japanese encephalitis-associated neuroinflammation. Methodology & Theoretical Orientation:Cultured neuron/glia containing 40% neurons, 40% astrocytes, and 20% microglia were prepared from cerebral cortices of Sprague-Dawley rats. JEV NT113, a JEV strain isolated from mosquito, was propagated in C6/36 cells and used to infect cultured neuron/glia. Findings: Infection of cultured neuron/glia with JEV caused a profound reduction of neuron viability and the neurotoxicity was attenuated by DHA. The neuroprotective action of DHA was accompanied by inhibition of NO, TNF-a, and IL-1b production. Mechanistic studies revealed that JEV infection elicited an elevation of toll-like receptor 7 (TLR7)/MyD88 signaling axis leading to activation of NFκB and AP-1 as well as expression of cytokines. We further identified that HMGB1 released from JEV-infected neuron/ glia had neurotoxic effect and proinflammatory effect. In conclusion, current results indicate that DHA can attenuate JEV infection- induced neurotoxicity involving resolution of neuroinflammation.
Biography Chun-Jung Chen has devoted his effort in the study of viral encephalitis and neurological degeneration. By culturing primary neural cells and establishing diseased models from rodents, pathological mechanisms and therapeutic strategies are his interests. He has built many types of primary neural cells and diseased animal models of central and peripheral nervous system after years of experience in research, evaluation, teaching and administration both in hospital and university.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 62 Yu-Hsiang Kuan et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Protective effect of 5,7,3'-trihydroxy-4'-methoxyflavone on LPS-induced acute lung injury via Akt- dependent NFκB pathway Yu-Hsiang Kuan and Ming-Ling Yang Chung Shan Medical University, Taiwan
cute lung injury (ALI) is the acute and serious inflammatory disorder which has the high incidence and mortality in patients. AHowever, there is still no effective treatment strategy for ALI up to now. 5,7,3'-trihydroxy-4'-methoxyflavone (THMF) has bioactive effects such as antifungal, antibacterial, antioxidant, anti-inflammatory effects. Acute lung injury was induced by intratracheal injection of LPS, and Diosmetin at various concentrations was injected intraperitoneally 30 min prior to LPS. Expression of cytokines and adhesion molecules were determined by commercially and by ELISA assay kits, respectively. Akt phosphorylation and NFκB activation were measured by western blot and NFκB activation assay kit, respectively. Pretreatment with THMF inhibited the histopathological changes such as neutrophils infiltration, increased in alveolar barrier thickness, hemorrhage, and hyaline membrane formation occurred in lungs in LPS-induced ALI. In addition, not only LPS-induced expression of cytokines was suppressed by THMF, but also adhesion molecules expression lungs were inhibited as well. Moreover, pretreatment with THMF reversed the Akt phosphorylation and NFκB activation induced by LPS. These results from present study suggested that the protective mechanisms of THMF on LPS- induced ALI were via Akt-dependent NFκB pathway.
Biography Yu-Hsiang Kuan has his expertise in evaluation in anti-inflammation, genotoxicity, and improving the pulmonary disease. He has developed the cellular and animal model based on acute lung inflammation, macrophages genotoxicity and cytotoxicity, and cellular signaling. He has built this model after years of experience in research, evaluation, and teaching in the education institutions.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 63 Sang Don Ryu et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 in dairy foods and environments Sang Don Ryu, Mi Ri Park and Sangnam Oh Chonbuk National University, Korea
he entry route of foodborne pathogens including EHEC O157:H7 from the dairy farm environment into raw milk and dairy Tfoods may come from several different vectors, and influence on the human health. Here, we employed HT-29 intestine epithelial cells (IECs; in vitro) and C. elegans (in vivo) as host model systems and compared the specific host responses during EHEC O157:H7 infections using whole transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157, we employed quantitative real time-polymerase chain reaction (qRT-PCR), transgenic worms, and RNAi. After EHEC O157:H7 infections, the regulation of gene encoding detoxification process, stress response, immune response and oxidative stress-associated proteins were immediately remodeled. Among them, we showed that the genes encoding cytochrome P450 (CYP450) family were dramatically induced by >10- fold during infection of EHEC O157:H7 is common in both host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infections comparing to wild-type N2. Consistent with susceptibility tests, qRT-PCR results showed that CYP450 loss of function mutations significantly affected the transcriptional induction of antimicrobial peptide genes such as clec-60. Taken in their totality our results provide critical insights for host strategy to exclude EHEC O157:H7 pathogenesis in gastro- intestinal (GI) tracts through cytochrome P450 family.
Biography Sang Don Ryu has great experience on detection and characterization of foodborne pathogens in food microbiology research. In recent, he focused on the prevalence and molecular pathway of various foodborne pathogens including Listeria monocytogenes, Staphylococcus aureus, Camphylobacter jejuni, Salmonella typhumurium, and pathogenic E. coli isolated from dairy foods and environments. Especially, his outstanding results may help monitor and control the spread of bacterial species through the farm environment, which is a natural reservoir for these organisms.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 64 Mi Ri Park et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Functionality of milk fermented with Lactobacillus spp. on metabolic bone health in ovariectomized rats Mi Ri Park, Sang Don Ryu and Younghoon Kim Chonbuk National University, Korea
he osteoporosis primary definition is a skeletal disorder of post-menopausal women or of old people. But, recent definition is, a Tsystemic skeletal disease characterized by low bone mass and the micro architectural deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture. Here, we focused on evaluating the noble functionality of fermented milk with selected probiotics for anti-osteroporosis using in vitro and in vivo assays. Among selected probiotic bacteria, Lactobacillus plantarum B719 exhibited higher (p<0.05) proliferation in MC3T3-E1 osteoblast cells, and was thus selected for subsequent analysis, involving for the comparison of osteoprotective effects with L. plantarum B719 fermented milk product (FMP-B719). Furthermore, the effects of FMP-B719 were investigated with medically ovariectomized (OVX) rats at the age of eight weeks. Results also demonstrated that assessment items for bone health including bone mineral density (BMD) in FMP-B719 group are significantly improved compared to that of OVX groups (p<0.05). Interestingly, metagenome analysis showed that the taxonomic composition at the phylum level showed a considerable alteration in fecal microbiota induced by FMP-B719. Taken together, we proposed that FMP-B719 could be a potential candidate for prevention of osteoporosis, improvement of bone remodeling as well as inhibition of bone resorption through influencing dynamics of gut microbiota.
Biography Mi Ri Park is a PhD candidate and has great experience on functionality of dairy originated foods including milk and probiotics for promoting human health. In recent, she is focusing on the molecular mechanisms of probiotic strain and its fermented products for anti-aging activity. In addition, she has interests in central nervous system (CNS) function by modulating signaling pathways of the microbiota-gut-brain axis. She has taken a novel viewpoint in this review by specifically highlighting that the gut microbial composition may be a key player for various metabolic diseases, and this may thus have critical impacts on CNS function, with significant implications for brain function and behavior.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 65 Seong-Soo Roh et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Evaluation of immunopotentiation activities of combined extract of silkworm and food in vitro and in vivo Seong-Soo Roh, Su Hyun Kim and Ah Reum Lee Daegu Haany University, South Korea
e conducted experiments about immunopotentiating with powder of silkworm as food and immunity promoting materials W(Angelica Gigas Root, Eucommiae cortex, turmeric, cinnamon, Alpinia oxyphylla Miquel, Achyranthes bidentata Blume, liriope radix, Japanese lady bell, chives, ulmoides). In order to measure the effect of enhancing the immune function, a sample of silkworm powder and composite materials were orally treatment for 2 weeks and T lymphocyte proliferation and IL-2, IL-4, and IFN-gamma secreted by T lymphocyte activation, degree of nitric oxide uptake and total spleen, lymphocyte, and peritoneal macrophage counts were measured in macrophages. That is, in vitro cytotoxicity was observed when the cells were co-cultured in silkworm powder and immunoprecipitation candidate treatment for 24 hours. As a result among 10 kinds of immunopotentiating material, 100 ug/ml Eucommia ulmoides, 400 ug/ml, 200 ug/ml cinnamon, 100 ug/ml liriope radix, 100 ug/ml chives, and Acanthopanax 100 ug/ml were chosen based on the results of spleen cell proliferation ability. The control group treated with Concanavalin A (Con-A) for activating splenocyte T cells and the control group treated with Con-A and candidate material samples were simultaneously treated to determine whether synergic effect of T cell proliferation occurred. As a result, group treated with each combination extract of Silkworm and Liriope radix, Cinamori cortex and Eucommiae cortex showed a synergistic effect on splenic T cell proliferation compared to group only treated with Silkworm. In addition, the expression levels of cytokines were increased in the silkworm cinnamon, silkworm liriope radix , and silkworm Eucommia ulmoides treated groups compared with silkworm alone group. In conclusion, it can be concluded that an equal amount of silkworms and mosquitoes can be obtained to enhance the immunity-enhancing effect.
Biography Seong-Soo Roh His major is traditional Korean medicine, and he has lectured and studied pharmacology and toxicity in Korean medicine at Daeguhaany university. In addition to natural drug research, he is also focusing on research on functional food ingredients and natural cosmetic materials. Now he is the editor-in-chief of The Korea Association of Herbology and serves as an academic member and editor of internationally renowned journals. He has built this model after years of experience in research, evaluation, teaching and administration both in hospital and education institutions.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 66 Diana Mareta I F A Pitaloka, J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Case study: Nutritional treatment for status epilepticus with ketogenic diet Diana Mareta I F A Pitaloka Taipei Medical University, Taiwan
Introduction: Status epilepticus is a condition which has seizures more than 30 minutes without recovery between them. Seizures in the meningoencephalitis patients are caused by enhancement of emphasis and expansive of inflammation area in the brain. The ketogenic diet was given to the epileptic patient to minimize the seizures through some pathways such as: inhibit neurotransmitter release and activation of ATP-sensitive potassium channels, enhance overall bioenergetics and reduce oxidative stress, and modulate tricarboxylic acid flux. Objective: The aim of this case study is to know the effectiveness of ketogenic diet in order to decrease seizures in a patient with status epilepticus. Method: This study was a case study which observed a patient (boy, 10 years old) at the Dr. Saiful Anwar Hospital, Indonesia, with status epilepticus and loss of consciousness. The ketogenic diet was given gradually, for 7 days (February 25th- March 3rd, 2015), a patient was given pan enteral diet 1200 cc/day (1200 kcal, protein 36.72 g, fat 61.8 g, and carbohydrate 130.8 g), for 9 days (4th-12th March 2015) received ketogenic diet 1600 cc (2169.9 kcal, protein 16.32 g, fat 107.2 g, and carbohydrate 280 g). Result: Seizure decreased at the first day after ketogenic diet administration (seizures < 30 minutes) and stopped completely at second day after administration. Based on monitoring result, a patient had decreasing body weight and MUAC during ketogenic diet administration. Furthermore, patient had enhancement of lipid profile (total cholesterol 211 mg/dL, TG 256 mg/dL, HDL 32 mg/dL, dan LDL 146 mg/dL). Conclusion: The ketogenic diet gradually can reduce and inhibit seizures in the meningoencephalitis patients.
Biography Diana Mareta IFA Pitaloka has completed her graduation in Nutrition and Health from Brawijaya University and currently is a Master’s student of Taipei Medical University (TMU), Taiwan. She got basic knowledge about clinical nutrition, food science, and community nutrition at her Bachelor’s degree level. Out of which, she is interested in clinical nutrition and food science. In her Master’s program, she concentrated on high fat diet, inflammation, and lipid metabolism. Recently, she learnt about glutamine and arginine against lipid metabolism in the liver.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 67 Nindy Sabrina et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
The Interaction between Iron and Nε-(carboxymethyl)lysine and its association with non-alcoholic fatty liver disease Nindy Sabrina and Jung-Su Chang Taipei Medical Universty, Taiwan
Background: Dysregulated iron metabolism is a common disorder in non-alcoholic fatty liver disease (NAFLD). Recently, the advanced glycation end products (AGEs) and it’s receptors (RAGE) are also linked to the pathogenesis of NAFLD. The aim of this study was to investigate the interactive effects of iron-AGE axis on fatty liver severity. Methods: NAFLD was diagnosed based on abdominal ultrasonography. In total, 170 Taiwanese adults (33 controls and 137 NAFLD) were selected for analysis. Parameters for RBC, iron status, soluble CD163 (sCD163), cell free hemoglobin (Hb) and Nε-(carboxymethyl) lysine (CML) were evaluated. Results: Multivariate linear regression analysis confirmed that serum free Hb (Beta=-1.1(-1.4--0.74); p<0.0001) and soluble CD163 (Beta=0.053 (0.01-0.09); p<0.05) are independent predictors of serum CML. Interactive analysis showed that, compared to those with the lowest sCD163 (T1) and free Hb (T1), individuals with the highest free Hb tertile (T3) and the lowest sCD163 tertile (T1) had an negative predicting effect on serum CML (Beta= -93.136 (-185.1—1.177); p =0.047) and the inverse relationship changes with increasing tertile groups of sCD163 (Beta=-46.54 (-139.2-46.1) and 65.1 (-12.6-142.84) for T2 and T3, respective; p-trend=0.011). Categorical logistic regression analysis showed that, compared to individuals with the lowest serum free Hb and CML median (M1, Reference), individuals with the highest serum CML (M2) and free Hb (M2) had 6.86 times (95% CI: 1.85-125.4; p=0.004) times highest risk for NAFLD and the odds increased to 8.66 times (95%CI: 1.684- 44.48; p=0.01) after adjusting for age, gender and BMI. Conclusion: Our results raise the possibility that increased shedding of CD163 surface receptor may interfere with CD163+macrophage’s ability to recycle serum free Hb and increased free Hb level may lead to the endogenous formation of CML and liver injury.
Biography Nindy Sabrina is a second year master student in the School of Nutrition and Health Sciences, Taipei Medical University, Taiwan. Nindy is originally from Malang, Indonesia. She received her undergraduate education at Brawijaya University, Indonesia, with a degree in Nutrition and Health. Currently, she is actively involved in Indonesia Student Association in Taiwan. Nindy is broadly interested in human nutrition research particularly obesity, iron, and advanced glycation end products. In her master research, she is seeking to understand the relationships between dietary intake, serum iron biomarkers and advanced glycation end products in Non- Alcoholic Fatty Liver Disease (NAFLD) patient in Taipei.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 68 Yu-Ri Kang et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Enhancement of anti-hyperglycemic activity of Jeju onion (Allium cepa L.) extract by heat-treatment process, and purification of bioactive compounds Yu-Ri Kang1, Jong-Wook Lee2, Sung-Cheul Kim2 and Young-In Kwon1 1Hannam University, South Korea 2Kunpoong Bio, South Korea
nion (Allium cepa L.) is one of the most widely used vegetable as food or medicinal plant due to its health benefits. It has been Oreported that onion and its extracts have antibacterial, antidiabetic and antioxidant activity. In our previous study, we confirmed that inhibitory activities of some onion cultivars against rat intestinal α-glucosidase and porcine pancreatic α-amylase were increased by heat-treatment process (121°C, 30 min). In this study, heat-treated sample was purified by cation-exchange and gel-permeation chromatography. Its structural characteristics were investigated by nuclear magnetic resonance (NMR) spectroscopy (1H, 13C, H–H COSY, HSQC, TOCSY, and NOESY). Furthermore, we investigated anti-hyperglycemic activity of heat-treated Jeju onion (Allium cepa L.), which is one of the yellow onion species grown in Jeju (Korea), in normal Sprague-Dawley (SD) rat model. Heat-treated sample was purified by column chromatography using cation exchange resin to obtain a purified product of a powdered form. To confirm the structure and purity of the sample, purified sample was subjected to NMR analysis, LC-MS/MS, and HPLC system. The structure was elucidated on the basis of extensive spectroscopic analysis, including 1D, 2D NMR and ESI-MS. Non-heated onion had no significant change in postprandial blood glucose levels compared to control group in normal Sprague-Dawley (SD) rat model. Meanwhile, heat-treated onion significantly decreased the postprandial blood glucose levels 8.7% at 30 min (p<0.01) when compared to control group. These results suggest that development of heat-treatment process with onion may provide useful dietary strategy for managing postprandial hyperglycemia.
Biography Yu-Ri Kang is a Doctoral Student at Hannan University, Daejeon, Korea and her research productivity as a graduate student is outstanding. She had very balanced experiences on both study (Hannam University, Korea; Exchange program from Framingham State University, Framingham, USA, 2013 and 2015) and research field (number of manuscript and poster presentation). Her major study field is bioactive polyphenols from natural plant foods inhabited in Jeju and US area and emerging issues in modern Food Science studies, especially in US.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 69 Ki-Rim Kim, J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Effects of Prunus persica fruit on nicotine toxicity in animal model Ki-Rim Kim Kyungpook National University, South Korea
icotine is a major toxic component of tobacco used through cigarette smoking and has been recognized to result in oxidative tissue Ninjury as an important risk factor for cardiovascular diseases, lung-related diseases and cancers. Prunus persica fruit has been used for the treatment of degenerative disorders such as hypermenorrhea, dysmenorrhea and infertility in Asia region including China, Korea and Japan. In this study, we investigated the protective effect of Prunus persica fruit extracts (PPFE) against chronic nicotine- induced tissue damages in animal model. Male ICR mice were injected intraperitoneally with nicotine (5 mg/kg body weight) and then orally administered with saline or PPFE (250 or 500 mg/kg body weight) once daily for 36 days. Chronic nicotine administration induced nephrotoxicity and hepatotoxicity in mouse serum. Nicotine also caused an increase in level of malondialdehyde (MDA) as a lipid peroxidation product, and decreases in glutathione (GSH) level and activities of antioxidant enzymes, including superoxide dismutase, glutathione peroxidase and catalase, in liver and kidney tissues. PPFE treatment, however, decreased MDA level, and recovered GSH level and activities of antioxidant enzymes. In addition, PPFE inhibited nicotine-induced histological changes and nitrotyrosine expression in tissues. These results suggest that PPFE may protect against tissue damages chronically exposed to nicotine.
Biography Ki-Rim Kim is a student and an affiliate of Kyungpook National University, South Korea
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 70 Amanda Berhaupt-Glickstein et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Older green tea consumers in the United States Amanda Berhaupt-Glickstein and William K Hallman The State University of New Jersey, USA
Statement of the Problem: By 2030, one in five Americans will be 65 years or older. As adults age, the risk for chronic disease, such as cancer, dramatically increases. Education programs and marketing campaigns should target this key demographic to prevent chronic disease and facilitate successful aging. Research shows that older adults are receptive to new information and adopt disease preventive behaviors, such as reading food labels and taking dietary supplements. The purpose of this study is to identify the demographic and psychographic characteristics of older green tea consumers in the United States. By understanding this segment’s background, perceptions, and behaviors, health and marketing professionals can tailor messages to reach clients and consumers. Methodology: An online survey was completed in January 2014 with a nationally representative sample of adults aged 55 years and older (n=1,335). Data were analyzed using descriptive statistics and binomial logistic regression. Findings: More than half (n=682, 51.2%) of respondents drank green tea. Most consumers are college-educated and employed, female home owners. The odds for green tea consumption are greater if a respondent is in good health, is informed about diet and health, or made a health-related dietary change in the past year. There are greater odds of consumption if a person is familiar with the relationship between drinking green tea and the reduced risk of cancer. However, the importance of health claims on product labels did not predict consumption. Health educators, regulators and marketing professionals may use this profile to tailor messages that speak to consumers and client’s values and motivations.
Biography Amanda Berhaupt-Glickstein is a Nutrition Research Consultant in Tokyo, Japan. She is a Registered Dietitian and has a PhD in Nutritional Sciences from Rutgers, The State University of New Jersey. Her research focuses on perceptions of nutrition marketing and claims made on food and dietary supplement labels. Her research interest is to help consumers make informed decisions through systems that characterize or suggest the nutritional quality of products. She currently serves as the Country Representative of Japan for the American Overseas Dietetic Association.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 71 Min Hyun Suk et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
The food intake status according to obesity among Korean adolescents Min Hyun Suk1, Yeojin Im2, Won-Oak Oh3 and Je Hyeong Kim3 1CHA University, Korea 2Kyung Hee University, Korea 3Korea University, Korea
Backgrounds: Adolescence refers to the psychosocial, emotional, cognitive, and moral transition from childhood to young adulthood. Adolescence offer health care providers unique opportunities for health promotion and preventive services. Purpose: The purpose of this study was to provide basic data for strategies in maintenance of optimal body weight among adolescents. Methods: This study was a descriptive study based on a secondary analysis of raw data from the th9 Korea Youth Risk Behavior Web- based Survey completed in 2013. The survey has been conducted annually with a complex design to identify the current health status and health risk behaviors of Korean adolescents enrolled in middle and high schools. A total of 799 schools were participated in the survey, and, in total, 72435 students responded (response rate: 96.4%). The adolescents were divided into low weight, normal weight, overweight, and obesity groups according to body mass index (BMI). Differences in food intakes were analyzed. Results: In obesity group, breakfast skipping rate was highest (χ2=27.13, p<0.001). Fruits and vegetable intakes were lowest in low weight (χ2=177.79, p<0.001) and normal weight (χ2=26.43, p<0.001) groups, respectively. Low weight group significantly intakes more carbonated soft drink (χ2=42.24, p<0.001), fast food (χ2=40.59, p<0.001), ramen (χ2=314.29, p<0.001), and snacks (χ2=641.87, p<0.001) than other groups. Conclusions: It was expected that intake of fast food, carbonated soft drink, ramen, and snacks would be higher in overweight and obesity groups. However, the results of this study showed that the intakes of fast food, carbonated soft drink, ramen, and snacks were higher in low weight group in difference with usual expectation. Therefore in the development of programs or policies for maintenance of optimal body weight among adolescents, the real status of food intake should be carefully surveyed and considered.
Biography Min Hyun Suk has her expertise in teaching and research in Community Health Nursing. Her main study involves figuring out the real health status and affecting factors, and developing strategies in health promotion among adolescents and elderly people.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 72 Ji Hyun Moon et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Low muscle mass and depressed mood in Korean adolescents: The fourth and fifth Korea national health and nutrition examination surveys Ji Hyun Moon, Mi Hee Kong and Hyeon Ju Kim Jeju National University Hospital, Korea
uscle mass and muscle function are related to depressed mood in studies of adults. Like older adults, Korean students are highly Mlikely to suffer from decreased muscle mass due to social conditions. In this study, we evaluated the muscle mass status of Korean adolescents and assessed the effect of muscle on depressive mood. A total of 1,233 adolescent male and female participants from the Korea National Health and Nutrition Examination Survey (KNHANES) were enrolled in our study. Participants underwent dual- energy x-ray absorptiometry (DXA) for assessment of appendicular muscle mass and completed self-report questionnaires regarding depressed mood, stress, suicidal ideations, and attempts. We found that 17.5% of boys and 10.9% of girls had low muscle mass (LMM). Boys’ depressed mood was unrelated to muscle status (P=0.928). However, girls with decreased muscle mass had a greater tendency for depressed mood compared to girls with optimal muscle mass (P=0.017). After adjusting for age, body mass index, smoking status, alcohol consumption, frequency of physical activity with resistance exercise, sleep duration, and stress, the female LMM group had a 4.05 greater risk than normal muscle girls of suffering from depressed mood (95% C.I.: 1.13-14.54, P=0.032). Adolescent girls who have insufficient muscle mass are more likely to report depressed mood than girls who have ideal muscle mass. Interventions for maintaining proper muscle mass are needed.
Biography Ji Hyun Moon is an affiliate of Jeju National University Hospital, Korea.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 73 Chew Wen Qi et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Absolute quantitative real-time PCR-based method for fish and fishery products authentication Chew Wen Qi and Cheng-Hong Hsieh Asia University, Taiwan
ish has been part of our food since few hundred million years ago. Chinese even believe that eating fish head will make a child Fsmarter, showing the great importance for fish to serve as an essential source of nutrients. The consumer can recognize fish when it maintains its complete profile but if it is cooked or processed, it is hard to do the identification. Unfortunately, fish adulteration causes fish recognition even harder. It is prevalent and risking the public health, whereby the risks include food poisoning, long-term health defects and so on. Therefore, quantitative real-time PCR (qPCR) has been devised in order to curb this issue. qPCR has been widely used due to numerous advantages such as high accuracy and high reliability. Compare to relative quantification, qPCR provides an absolute genome copy number for a specific target. Hence in this study, we developed a crucial real-time quantitative PCR for the detection of common edible fish species in Taiwan that are usually processed or adulterated. The genome of generic fish fragments was used as a standard, alongside with the design generic primers and probes for the fish, to calculate the genome copy number of fish. Finally, we demonstrated the efficacy of quantitative real-time PCR for the authentication of common edible fish and fishery products in Taiwan. In conclusion, this assay enables multiple fish species genome copy number being detected, which could be applied to detect adulterated foods and genetically modified foods in general.
Biography Chew Wen Qi is an affiliate of Asia University, Taiwan.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 74 Yi-Cheng Hou et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Brain coordination and the association with diet in prediabetes Yi-Cheng Hou1, Jiun-Rong Chen1, Shwu-Huey Yang1, Yu-Te Wu2, Cheng-yu Chen3 and Chien-Han Lai2 1Taipei Medical University, Taiwan 2National Yang-Ming University, Taiwan 3Taipei Tzu Chi Hospital, Taiwan
he functional connectivity of diabetes can help us explain the brain function decline in hyperglycemic status. However, the issue Thas not been addressed much in prediabetes. Therefore, we designed this study to investigate the inter-hemispheric coordination in the prediabetes. Sixty-four prediabetic patients and fifty-four controls were enrolled in this protocol. They received the structural and resting-state functional magnetic resonance imaging screen. The imaging data were preprocessed and analyzed to obtain voxel- mirrored homotopic connectivity (VMHC), which can measure inter-hemispheric coordination. The VMHC values were compared between two groups with age and gender as covariates. The controls had higher VMHC values than prediabetic patients in bilateral anterior cingulate cortex. The prediabetic patients had higher VMHC values than controls in bilateral middle frontal gyrus. The VMHC values were also negatively correlated with pre-prandial serum glucose level in inferior frontal gyrus of prediabetic patients. In addition, the VMHC values of prediabetic patients were negatively correlated with total carbohydrate and calorie intake in anterior cingulate cortex. The inter-hemispheric coordination in anterior sub-network of default mode network and fronto-cingulate regions would play a role in the pathophysiology of prediabetes. The diet impact on the inter-hemispheric coordination is also an interesting issue.
Biography Yi-Cheng Hou beening detention since June 2007 in Taipei Tzu Chi Hospital. So far, in the clinical business in the deep understanding of pre-diabetes and diabetes patients have an increasing trend for the active intervention of this group of patients become urgent of the subject. Diabetes and pre-diabetes in addition to blood sugar than the average person, the nutritional intake and diet behavior correction, intestinal function has begun to occur lesions, and even whether the brain structure has begun to change, need to be strictly monitored. Therefore, Hou dietitian research is mainly for pre-diabetes and diabetes patients with the above objectives of the intervention. During the working period, two papers have been published in the domestic society, ten international papers, one from SCI original papers and international journals SCI papers
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 75 Seok Jun Son et al., J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Analysis of microbial community and functional components of Kombucha Seok Jun Son1, Hyung Joo Suh2, Jung Min Bae1, Sung Hee Han2, Sin Geun Kim2 and Hyun-Sun Lee1 1Agency for Korea National Food Cluster, Korea 2Korea University, Korea
ombucha is a fermented beverage obtained by fermentation of bacteria and yeast; green tea (or black tea) and sugar as main Kingredients. It is also known as ‘mushroom tea’ and it has been used as a folk remedy for hepatitis, arthritis, indigestion and hypertension. However, scientific data on its efficacy is very low. Therefore, we identified the major microbe’s influence to ferment the Kombucha and studied to establish the optimum conditions for Kombucha during the fermentation process. We analyzed the microbial community after Kombucha fermentation using the Kombucha as a starter. In group A,Komagataeibacter hansenii was 42.09% while in group B, Komagataeibacter hansenii was 90.56%. In addition, after the identification analysis, the major strains of Kombucha were acetic acid bacteria (Gluconacetobacter hansenii, Gluconacetobacter europaeus, Gluconobacter oxydans, Acetobacter peroxydans) and yeast (Dekkera bruxellensis, Lachancea fermentati, Cryptococcus diffluens). As a result of the analysis of various components during the fermentation process of Kombucha, the amount of dried matter and amino nitrogen of the culture tended to decrease with increasing fermentation time. Changes in total sugars tended to decrease with increasing fermentation time, while changes in the content of reducing sugars tended to increase with fermentation time of Kombucha. Moreover, ABTS radical scavenging activity was not changed during the fermentation process while DPPH radical scavenging activity was slightly increased with increasing fermentation time. The main organic acid of Kombucha was acetic acid, and glucuronic acid; malic acid and lactic acid were also produced. Through our scientific analysis, we anticipate that Kombucha result in the discovery of many more potential functional beverages for food industry.
Biography Seok Jun Son is a PhD candidate and has great research experience on functionality of diverse foods or its materials including fermented beverage (Kombucha), royal jelly, protein powder originated rice, and probiotics for promoting human health. Recently, he is focusing on the molecular mechanisms of probiotic strain and its fermented products for intestinal microbial community and anti-inflammatory properties. In addition, he has taken a novel viewpoint in this review by specifically highlighting that the microbial composition in beverage (Kombucha) may be a key player for fermentation products and its functionality. Especially, his outstanding results may help monitor and control the microbes and fermentation products through the producing environment, which has influence on these foods.
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J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
The relation between jamu (traditional herbs) consumption and food restriction (taboo) against hemoglobin level of preconception woman in Banggai regency, Central Sulawesi Province Lucy Widasari1, Yustiyanty Monoarfa2, Rahayu Yekti1, June Luhulima1, Anang S Otoluwa3, Maisuri T Chalid1, Nurhaedar Jafar1, Veni Hadju1, Soekirman1, Abdul Razak Thaha1 Hasanuddin University, Indonesia Airlangga University. Indonesia Banggai District Office. Indonesia Indonesian Nutrition Foundation for Food Fortification, Indonesia
Background: Nutritional status during preconception period is an important determinant of pregnancy outcome. Cultural norms, taboo, and beliefs lie within the contextual factors that could potentially affect nutritional status and health in preconception women. Methodology: The research was conducted in threesub-districts of Banggai district, namely, Luwuk, North Luwuk, and South Luwuk, from October 2016 to February 2017. This study aims to identify relation between Jamu consumption (traditional herbs) and food restriction (taboo) against hemoglobin (Hb) value in preconception women in Banggai Regency in the year 2016-2017. This study used cross sectional design with saturated sampling technique. The populations in this study were 53 preconceptional women that meet the inclusion and exclusion criteria to judge the several factors that affect the value of Hb. Bivariate analysis using unpaired t test. Results: The result show that stated hemoglobin levels average is 12.76 g / dL and the mean value 13.0 g / dL with the lowest hemoglobin value of 7.3 g / dL and hemoglobin highest value 15.9 g / dL. The test results of bivariate showed no significant difference in mean hemoglobin values among women whose consume herbs and women who did not (p = 0.751), there was no significant difference in mean hemoglobin values between groups of women with certain food restriction (taboo) and those who do not have restriction on certain foods (p = 0.231), there are no significant differences in mean hemoglobin values among women who do not consume herbs and those who consume herbs (p = 0753). Conclusion: It is important to be aware of the pharmacological content of the medication particularly when it is systemically administered. Food taboos influence the amount, frequency and quality of nutrients as contributing factor against hemoglobin level and nutritional status of women before pregnancy. Hence, we need integrated and comprehensive approaches, with interventions to improve the overall health of the preconception women in this specific cultural context.
Biography Lucy Widasari is a Doctoral Candidate and an affiliate of School of Public Health, Hasanuddin University, Indonesia.
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10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
A novel real-time PCR quantitative method of crab species Zi-Yu Song and Cheng-Hong Hsieh Asia University, Taiwan
eat adulteration has posed considerable risks to public health, and quantitative methody real-time PCR (qPCR) is a powerful Mtool used for both research and diagnostic, which has the advantages, compared to relative quantification, of providing an absolute copy number for a particular target. However, reliable standard is important for qPCR by using genome synthesis of generic crab fragments as a standard (fragments region is 16s rRNA) . In this study, we developed a novel real-time PCR quantitative method for the detection of crab species that are used as meat products or meat adulterants, And design generic primers and probes for crabs, and then calculate copy number of crabs. The results confirmed crab species could be tested to calculate their copy number. The limit of quantification was 12 copies of crabs genomic DNA or 1% meat ingredient. This assay enables detection of multiple species of crab copy number. This is also applicable to other adulterated foods and genetically modified foods.
Biography Zi-Yu Song graduated from the Asia University of Food Nutrition and Biotechnology, after graduation continue to study, studying at the Asian University Master student. Because Taiwan is surrounded by the sea, the sea food is very rich in resources, many people poisoning in Taiwan because of eating toxic crab. So my direction of the study of the crab qualitative, quantitative, and calculate the copies, the future will continue to study crab processed products, and combined with the detection of toxic crabs(TTX), the future hope that I can make effort to protect the Taiwanese food safety issues.
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conferenceseries.com 963rd Conference
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Accepted Abstracts
Scientific Tracks & Abstracts
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Challenges in clinical nutritional research: How adaptive design can help? Chow Shein-Chung2, Tanguy Jérôme1, Gosoniu Maria Laura1 1Nestlé Research Center, Switzerland 2Duke University School of Medicine, USA
uman studies are essential to obtain evidence of beneficial effects of nutritional interventions on health. Clinical nutritional Hdevelopment plans include a range from small pilot trials to well-designed confirmatory studies, following good clinical practices. Nutrition interventions are generally safe compared to pharmaceutical compounds. Regulatory guidance limits the benefits of nutrition to the maintenance of normal function or the reduction of accepted risk factors. Therefore, nutritional trials do not necessarily follow the same development process as pharmaceutical products. The objectives focus more on maintaining health and preventing risk factors for disease rather than showing efficacy in disease conditions. Clinical trials in the nutritional field have specific challenges to overcome in terms of population, intervention, design and methodology. Additional challenges are the lack of learning phases, small beneficial effects, high heterogeneity of the responses and difficulties in finding appropriate endpoints. In recent years, the use of adaptive design methods in clinical trials has received much attention due to its flexibility and efficiency in clinical development. In practice, adaptive design may provide the opportunity to modify certain aspects of the trial design whilst the study is still ongoing, without violating the quality and the integrity of the data. However, introducing more flexibility may come with many difficulties: major adaptations of on-going trials may result in designs unable to address the original scientific/medical questions. In addition, fundamental differences between pharmaceutical and nutritional research potentially trigger limitations but also opportunities for the application of such innovative design. The aim of this work is to highlight specificities and challenges in nutritional clinical trials, review the concepts of adaptive design and then address the advantages, limitations, and feasibility of commonly considered adaptive designs. Statistical, clinical, operational and regulatory aspects of implementing adaptive designs will be discussed.
New trends within the probiotics market: how producers should deal with them Daniel R Calvo Biopolis, Spain
t is undeniable the fact that the probiotic market is suffering a transition where new trends are coming across. In addition to this, Iregulation (by means of EFSA in Europe) is extremely relevant. New trends and Health Claim Regulations entail new challenges and impact on innovation. Furthermore, the industry is moving at a fast-growing pace so that products containing probiotics are attractive to both food and pharma companies. New indications from different nature -the so called third generation claims- appear more and more as hot topics/niches (atopic dermatitis, psoriasis, stroke, sport nutrition, gut-brain axis), abandoning soft communications such as digestive health or immune system support. In this sense, new and interesting final product ideas are being developed within the industry, where probiotic producers should acquire a relevant role, going together with pharma and food industry based companies as well as contract manufacturers in order to create outstanding synergies and fulfill new market needs.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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Dynamic of human adipose tissues and ectopic fat deposits induced by different lifestyle interventions: a long-term MRI randomized trial Iris Shai Ben Gurion University, Israel
Background: It remains unclear whether specific lifestyle strategies can differentially mobilize fat depots/ectopic-fat-deposits. Methods: During an 18-month trial in an isolated workplace with monitored, provided lunch, we randomly assigned participants with abdominal obesity/dyslipidemia to iso-caloric low-fat (LF) or low-carbohydrate/Mediterranean(LC/MED) diets. After 6 months, we re-randomized half of each arm to moderately-intense physical-activity (PA+;80%-aerobic; free gym membership). We performed whole-body magnetic-resonance-imaging (3-Tesla-MRI) at 0,6,18 months. Results: Of 278 randomized participants [age= 48 yr; 88% men; body-mass-index= 30.8kg/m2) 86.3% completed the trial. While the caloric deficit was similar, the LF group preferentially decreased saturated/unsaturated fats (p<0.001), and the LC/MED decreased carbohydrates (p<0.001) and trans-fat intake (p=0.029 vs. other diet). The PA+ group increased their MET/wk (p=0.009 vs. PA-). Fat depots/deposits were significantly reduced to different degrees [Hepatic: (-32%); deep-subcutaneous-adipose-tissue (SAT): (-29%); visceral-adipose-tissue (VAT): (-25%); superficial-SAT: (-19%) and intra-pericardial: (-14% (-25mL))], with lesser changes of renal: (-9%); intermuscular: (-2%) and pancreatic-fat: (-1%). Although weight loss was comparable, LC/MEDPA+ favorably reduced waist- circumference (p<0.05), and induced -14% further loss of intra-pericaridal; p=0.003, -4% VAT; p=0.037 and -1.9% pancreatic-fat; p=0.007, compared to LFPA- changes (reference-group; weight-loss-adjusted for all). LC/MED decreased hepatic fat beyond LF diet by -15%; p=0.044. Conversely, renal-sinus and femoral-intermuscular fat dynamics were similarly altered by lifestyle strategies. VAT and intrahepatic fat loss associated with an improved lipid profile, and deep-SAT loss with improved glycemic control. Conclusions: Human depots/ectopic-fat-deposits are substantially variable in their capacity to respond to different long-term moderate weight-loss strategies. LC/MED diet, particularly with PA, can favorably enhance fat mobilization in several depots. These findings may suggest defined lifestyle protocols for targeting specific fat storage sites.
Immunomodulation by Food Vaibhav Aher Matoshri College of Pharmacy, India
n optimally active and balanced immune system is a pre-requisite for maintaining health. Immunity strongly varies at different Alife stages. Over past few decades, well balanced immune system for maintaining good health has been crucial. There is a strong consensus that nutrition plays a role in modulating immune function and that the immune system needs adequate supply of nutrients to function properly. The intricacy of the immune system supports this idea because its optimal functioning involves a variety of biological activities including cell division and proliferation, energy metabolism, anti-genotoxicity and production of proteins. The micronutrients most often cited as being important to immune function include vitamins A, C, E, and B6, folate, iron, zinc, and selenium. Other nutrients mentioned as playing a role in immune function include beta-carotene (a precursor to vitamin A), vitamin B12, and vitamin D. On the other hand, over-activation of the immune system can lead to detrimental effects such as chronic inflammation or autoimmune diseases. In persons with allergies, a normally harmless material can be mistaken as an antigen. This review will highlight the interaction between the immune system and some foods and food components in terms of modulation of immune functions by a variety of mechanisms.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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Effect of diet rich in lycopene and exercise on blood lipids in adults with dyslipidemia Cruz-Bojórquez Reyna María1, González-Gallego Javier2, Sanchez-Collado2, Pilar Avila-Escalante María Luisa1 1Universidad Autónoma de Yucatán, México 2Universidad de León, España
Objective: To determine the effects of a diet rich in lycopene, the practice of physical exercise or association between them on levels of blood lipids in adults with dyslipidemia. Material and Methods: It is an experimental study with duration of 12 weeks. The diet was designed rich in lycopene (50mg / day), the standard diet and exercise program. Before and after the intervention lipid profile of the participants was determined. Involved people between 21 and 64 years old, randomly divided into 4 groups: two standard diet (one did exercise and one not), and the other two with diet rich in lycopene, one did exercise and one not. For each indicator behavior regarding their group arithmetic mean and standard deviation were used. Later ANOVA was used to demonstrate the significance and finally the post hoc Tukey test. Results: 48 participants completed the study of which 35.4% (17) were male and 64.6% (31) female. The mean differences were significant post-pre cholesterol (p = 0.004) and LDL (p = 0.001). The mean difference between groups was significant for cholesterol between the standard diet with exercise and diet rich in lycopene with exercise and vice versa (p = 0.002). For the LDL was significant difference between the standard diet and exercise group with the other groups. Conclusions: The lycopene-rich diet (50 mg / day) for 12 weeks significantly reduce LDL cholesterol levels and blood. Exercise training (4 days a week for 60 minutes) for 12 weeks significantly decreased blood levels of LDL when combined with the standard diet and cholesterol levels and LDL to be associated with diet rich in lycopene.
The association between dietary intake of folate (F) and vitamin B12 (VB12) with body composition (BC) of adolescents aged 10-18 years from Merida, Mexico Avila-Escalante Maria Luisa1, Bogin Barry2, Cruz-Bojórquez Reyna María1, Dickinson Federico3 1Universidad Autónoma de Yucatán, México. 2Loughborough University, UK 3Unidad Mérida, México
exico has a high prevalence of stunting in children and adolescents, due to poor nutrition. Paradoxically, too many Mexican Mchildren and adolescents are currently overweight or obese (OW/OB). The dual-burden of stunting (low height for age) and OW/OB are nutritional conditions that increase the risk of developing chronic degenerative diseases in adulthood. The persistence of high levels of stunting in southern regions of Mexico, such as Yucatan, may be due to diet changes (nutrition transition) that Yucatecan population has experienced in recent years. Vitamins, such as folic acid (FA) and VB12 are micronutrients necessary for normal metabolism and growth. The relationships between the intake of these micronutrients and growth rate were identified. The impact of micronutrient consumption on body composition was also investigated. The sample population comprised 273 adolescents (152 females) aged 10-18 years, from Merida, Mexico. Dietary information was obtained using a food frequency questionnaire (FFQ) covering 106 local foods. Anthropometric and body composition measurements were taken, and socioeconomic data were gathered. Data were collected between September 2011 and July 2012. Over 50% of participants had inadequate consumption of both vitamins: 64% had inadequate intake of FA and 69% of VB12. Almost half (46.9%) were either short or stunted, and 33% were at risk of OW/OB. A higher risk of presenting OW/OB was found for participants with FA intake less than the RDI of 400 μg/day and for those with VB12 intake less than the RDI of 2.4 μg/day. No relationship was found between body fat percentage and the consumption of FA or VB12.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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The pleiotropic effects of fisetin and hesperetin on human acute promyelocytic and chronic myeloid leukemia cells Aysun Adan and Yusuf Baran Abdullah Gul University, Turkey
he studies elucidating the roles and the mechanisms of action of fisetin and hesperetin, plant flavonoids, in acute promyelocytic Tleukemia (APL) and chronic myeloid leukemia (CML) are absent. In this study, we investigated the mechanism of the antiproliferative and apoptotic actions exerted by fisetin and hesperetin on human HL60 APL and human K562 CML cells. The viability of HL60 and K562 cells was evaluated using the MTT assay, apoptosis by annexin V/propidium iodide (PI) staining and cell cycle distribution using flow cytometry, and changes in caspase-3 enzyme activity and mitochondrial transmembrane potential were determined. Moreover, we performed whole-genome microarray gene expression analysis to reveal genes and biological networks affected by KEGG and IPA analysis. For APL cells, both flavonoids showed a concentration- and time-dependent inhibition of proliferation and induced G2/M arrest and G0/G1 arrest for hesperetin at only the highest concentrations. There was a disruption of mitochondrial membrane potential together with increased caspase-3 activity. Furthermore, findcution of apoptosis was confirmed by annexin V/PI analysis. The microarray gene profiling analysis revealed some important biological pathways including MAPK and inhibitor of DNA binding (ID) signaling pathways altered by fisetin and hesperetin treatment as well as gave a list of genes modulated involved in cell proliferation, cell division, and apoptosis. For CML cells, fisetin and hesperetin inhibited cell proliferation and triggered programmed cell death. The latter was confırmed by mitochondrial membrane depolarization and an increase in caspase-3 activation. In addition to that, we have detected S and G2/M cell cycle arrests and G0/G1 arrest upon fisetin and hesperetin treatment, respectively. The microarray gene profiling analysis revealed some altered important signaling pathways including JAK/STAT pathway, KIT receptor signaling. They both significantly modulated the expression of genes involved in cell proliferation and division, apoptosis, cell cycle regulation, and other significant cellular processes such as replication, transcription, and translation.
Investigation of ferulic acid effects on autophagy Akin demet1, Farah adriana2, Demircan gunnur3, Beyhan ozdas sule3 1Bahcesehir University, Turkey 2Federal University of Rio de Janeiro, Brazil 3Istanbul Bilim University, Turkey
offee is particularly rich in bound phenolic acids, such as caffeic acid, ferulic acid, and p-coumaric acid. Over the past years, Cseveral studies have shown that Ferulic acid acts as a potent antioxidant by scavenging free radicals. Autophagy has recently been considered as a protective mechanism during the development of atherosclerosis because of its ability to stabilize plaques through the processing of oxidatively modified proteins. Our objective was to study the effect of ferulic acid on autophagy in cultured human umbilical vein endothelial cells (HUVECs) undergoing oxidative stress. LC3B and Beclin1 protein expression levels were measured by western blott and immunocytochemistry in oxidative stress conditions induced by administration of 200 microM hydrogen peroxide
(H2O2) for 1 hour. After H2O2 administration cells were washed with DMEM and incubated 24 hours with 100 microM ferulic acid (FA). Ascorbic acid, a known antioxidant, has been used as positive control. Ferulic acid decreased the ratio of LC3II/LC3I and Beclin-1 protein levels in oxidative stress conditions. We found similar results in immunocytochemistry studies. Our preliminary data suggest the novel cardioprotective properties of ferulic acid mediated by targeting the autophagic pathway. Ferulic acid may have the potential for use as an autophagic-related antioxidant for prevention and treatment of oxidative stress.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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Fortification of some date products with microencapsulated probiotic bacteria Saleh F A King Faisal University, Saudi Arabia
ecently, scientists interested in functional food, especially probiotic food which has many health benefits. Probiotic microorganisms Rexpose to many risks during their presence in food products and after passing through gastrointestinal tract as well. Therefore, the a main goal of this study to try to keep probiotic bacteria alive in date products during storage period and after eating. Free and calcium alginate microencapsulated Bifidobacterium lactis Bb-12 were mixed with date paste and date syrup (Dibs) and exposed to simulated gastrointestinal conditions, to observe its effects on viability of on B. lactis. The results showed that date paste and Dibs have a protective effect to the free bacteria and increased protection of encapsulated bacteria when compared to free bacteria without mixing with a date paste and Dibs. The viable counts of probiotic bacteria were numerated in date paste and Dibs during storage. At the end of the seventh week from storage period, the numbers of free B. lactis were reduced in both date paste and Dibs for up to 106 cfu/g, while the numbers of encapsulated bacteria remained at 107 cfu/g. The microencapsulated bacteria retained the numbers up to 106 cfu/g until week ten. The changes in pH values, production of organic acid and color of date paste and Dibs were monitored during storage period. The sensory analysis of date products fortified with probiotic bacteria were evaluated. The results showed that the addition of both free and encapsulated probiotic bacteria did not show any significant effect on the sensory properties of date products. This study has shown the microencapsulation protected Bifidobacterium lactis Bb-12 from the high sugars concentration of date products and simulated gastrointestinal conditions. Such of this product will provide an alternative probiotic food choice for consumers and could be particularly appealing to children.
Analysis of dose of fast food consumption as risk factor of childhood obesity in Yogyakarta, Indonesia Esti Nurwanti1, Hamam Hadi1 and Madarina Julia2 1Alma Ata Center for Healthy Life and Food (ACHEAF), Indonesia 2Gadjah Mada University, Indonesia
Background: The childhood obesity has been an epidemic in both developed and developing countries including Indonesia. Whether exposure to fast food consumption is associated with the increased obesity prevalence in Indonesian school children remains unclear. Objective: To examine dose energy, saturated fat, and sodium from fast food as risk factors of obesity in Indonesian elementary school children. Design: A case-control study of 244 obese and 244 non obese children was conducted in Yogyakarta municipality in 2012. Cases were elementary school children with body mass index/age > 95th percentile identified in previous survey. Controls were children from the same class and seating at the right side nearest to the cases. Weight and standing height were noted by trained nutritionists. Data on fast food consumption were obtained by semi quantitative food frequency questionnaire. Body mass index of the study subjects were computed using WHO Anthro2005 software. Statistical analysis was done using STATA 11th edition software. Result: Elementary school children who ate energy from fast food >1500 Kcal/day (OR=3.24) and saturated fat from fast food > 30 g/ day (OR=6.32) more likely to be obese. Sodium from fast food intake > 1500 mg/day also have significant result to increase obesity risk in children (OR=2.08), but the result is not consistent. Conclusion: Energy, saturated fat, and sodium from fast food were proven to be significantly associated with the increased risk of obesity in Indonesian elementary school children.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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Breakfast skipping as a risk correlate of overweight, obesity and central obesity among adolescents in the state of Qatar G Daradkeh1, Asma Al Muhannadi1, P Chandra1, Adam Fadlalla2, Moudi Al Hajr1 and H Al Muhannadi1 1Hamad Medical Corporation, Qatar 2Qatar University, Qatar
Background: Prevalence of overweight and obesity among children and adolescence over the past three decades reached an alarming point in both developed and developing countries. As a result of the economic boom overweight and obesity has become a major health problem in the Arabian Gulf countries particularly in Qatar. Breakfast is considered to be the first and most important meal of the day. Breakfast skipping may be one of the complexes and multi-factorial causes of obesity. Aims: The aims of this study were (1) to examine the prevalence of breakfast skipping among adolescent in the state of Qatar. (2) Evaluate the hypothesis that breakfast skipping would be associated with increased overweight and obesity and central obesity in the study population. Method: A cross-sectional study was carried out among 1225 adolescents (625 boys and 600 girls) aged 15 to 18 years were selected by means of a multistage stratified random sampling technique from 14 public and 7 private schools between October 2012 and February 2013. Anthropometric measurements: Weight in (kg), height in (cm), Body Mass Index (kg/m²), waist circumference (cm) and waist to height ratio (WHtR) were measured/computed. Demographic data and Food habits were collected by using structured questionnaire. Breakfast eating pattern was classified as:Breakfast eaters (students who are ate breakfast at least 4 days in a week). Breakfast skippers (students who skipped breakfast at least 4 days in a week). Results: Breakfast skipping is a common unhealthy habit, out of 1225 participants 766 (62.5%) consumed breakfast less than daily. 52.5% of the participants were breakfast skipper (ate breakfast less than 4 times/week). Weight, Body Mass Index (BMI), waist cir¬cumference (WC), was significantly different between participants age in both gender p< 0.05. The prevalence of overweight (43.0%) and obesity (39.5%) was lower among breakfast eaters than skippers (56.7% & 60.5%) respectively. In addition central obesity (WHtR ≥0.5) was more prevalent among breakfast skippers than eaters (59.3% vs 40.7%) respectively. A dose-response relationship was shown to exist between breakfast consumption and being overweight and obese among all students. This study showed that breakfast skippers were heavier than eater in both genders; body mass index was significantly higher among breakfast skippers. Conclusion: Regular breakfast consumption is negatively associated with overweight and obesity among adolescents in Qatar, we found that breakfast skipping was prevalent in our study sample, and was associated with overweight, obesity and central obesity in both gender. Awareness programs about the importance of breakfast meal are needed for adolescents.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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Nutrient quality of 22 important coldwater fish species and their potential contribution to human nutrition Debajit Sarma, P Sharma, M S Akhtar, C Akhtar, V Joshi, A Sharma, P Das, D Baruah and A K Singh ICAR-DCFR, India
utrient quality of 22 important coldwater fishes preferred by the people of upland region in their diet was analyzed and their Npotential contribution to human nutrition was evaluated. Tor putitora, N. hexagonolepis, O. mykiss, S. richardsonii and C. carpio were examined for muscle fatty acid and amino acid composition. All the fish species recorded excellent amounts of n-3 fatty acids (8.69– 21.48%) and the n-3/n-6 ratio ranged from 0.7 to 4.98. The amino acid score calculated based on the WHO protein standard indicated good quality proteins. Limiting amino acids such as lysine, methionine and cysteine occurred at levels higher than or marginally lower than the recommended amino acid level. Six small indigenous fish species namelyM. aral, Setipinna phasa, C. garua, A. morar, B. bendelisis and S. semiplotus were subjected to muscle nutrient quality. Protein and lipid in all six fishes were found ranging from 15.65 (S.phasa) to 20.88% (C. garua) and 2.91 (C. garua) to 13.23% (S.phasa) respectively. Among the fatty acids, sum of n-3 PUFAs was high in S. semiplotus, S. phasa and B. bendelisis, and lower in C. garua and so was in n-3/n-6 ratio. In most of these fishes, with slight exception, the profiles of macro minerals reveals the abundance trend, as potassium>calcium> sodium, while the trend for micro minerals was iron>zinc> manganese. In terms of dietary mineral contribution, M. aral and B. bendalisis showed best dietary potential for Ca and S. phasa and M. aral contributed for Fe. Also while analyzing the muscle composition of L. dero, L. dyocheilus, S. sanguina, B. bendelisis, G. mullya, L. pangusia, the important food fishes in Himalaya recorded crude protein levels ranged from 16-20%, crude fat 9.60-1.54%, moisture content 71-78% and ash 3.5-0.99%. Maximum concentration of potassium was found in L. dero followed by L. pangusia. Selenium was abundant in all the experimental fishes. Proximate composition, total fat, cholesterol, triglyceride, mineral and fatty acids profile of the flesh of five snow trout (S. niger, S. progastus, S. plagiostomus, S. curvifrons and S. esocinus) were also analyzed in the present study. SFA, MUFA, PUFA content were ranging from 57.47-66.06%, 19.44-31.66% and 10.54-14.51%. In SFA, palmitic acid (C16:0) is predominant followed by myristic acid (C14:0). Oleic acid (C18:1n9) was higher in S. plagiostomus (16.93%) and S. esocinus (13.61%) while palmitoleic acid (C16:1n7) was principal MUFA in S. niger (16.49%) S. progastus (13.66%), and S. curvifrons (14.34%). The dominant n-6 PUFA were linolenic acid (C18:3n6), linoleic acid (C18: 2n6) and arachidonic acid (C20:4n6). The DHA values are higher than EPA. The n-6/n-3 ratio ranged from 2.135-4.167. Phosphorus was maximum (5800-8300 mg/kg) followed by potassium (4500-7000 mg/kg), calcium (3500-4300 mg/kg), sodium (700-1800 mg/kg) and magnesium (700-1500 mg/kg). Trace mineral contents (mg/kg muscle) were observed 114.48-125.97 (Fe), 26.38-64.5 (Zn), 4.34-16.19 (Mn) and 7.16-18.11 (Cu). The above results infer that all the studied fishes are having excellent nutrient qualities in terms of protein, PUFA and selected micro and macro minerals which are beneficial to human health.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
Page 86 J Nutr Food Sci 2017, 7:4 (Suppl) conferenceseries.com DOI: 10.4172/2155-9600-C1-045
10th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan
Is there a correlation between eating habits and study location among Indonesian college students? Rany Adelina and Fatimah Azzahra Ministry of Health, Indonesia
Statement of the Problem: The background of this study is referring to the phenomenon of Indonesian students who live outside their hometown tend to have a careless eating habit. The difference in study location may cause a different type of eating habits between Indonesia and overseas, furthermore study intensity, meal preparation, and food access also may be influenced by this difference of study location. The purpose of this study is to determine the correlation between study location and eating habits for students who go abroad in Malang, Indonesia and in the city of Taipei, Taiwan. Methodology & Theoretical Orientation: Subjects were Indonesian college students who live outside their hometown for doing the study to Malang city, Indonesia and Taipei city, Taiwan. The total target sample was obtained as many as 493 respondents (397 respondents in Malang city and 96 respondents in Taipei City). Measured variables were studied intensity, meal preparation, food access, and eating habits in both two cities. Findings: The Indonesian college students, who lived in both Malang city and Taipei city, did not have the significant difference in eating habits. However, the trend showed healthier eating habits in Malang city. On the other hand, study intensity, meal preparation, and food access were significantly different in both cities. Indonesian college students in Taipei tended to have higher prevalence of low study intensity, dependent meal preparation, and difficult food access. Conclusion & Significance:There is the significant correlation between study location and study intensity, meal preparation, food access, but not eating habits. Future research is needed to know the reasons for this insignificant correlation between eating habits in these two countries.
Silkworm pupae oil exerts hypolipidemic and antioxidant effects in a rat model of high-fat diet-induced hyperlipidemia Yuxiao Zou Guangdong Academy of Agricultural Sciences, China
ilkworm pupae are good resources of edible oil that is especially rich in unsaturated fatty acids and considered an excellent dietary Ssupplement for hyperlipidemia. In order to investigate the effects of silkworm pupae oil (SPO) on the lipid profile and oxidative stress, dyslipidemic-diabetic Wistar rats (n=50) were divided into five diet groups of 10: normal control (C); high-fat diet control group (HF); and three other groups fed a high-fat diet with SPO supplementation (1, 2, or 4 mL•kg-1•d-1) orally. Groups fed with SPO had significantly lower concentrations of serum total cholesterol (TC) (P<0.05) and low-density lipoprotein cholesterol (LDL-C) (P<0.05) compared with the HF group. In regard to antioxidant parameters, except for levels of glutathione peroxidase (GSH-Px) in the liver, 2 and 4 mL•kg-1•d-1 of SPO supplementation leaded to higher total antioxidant capacity (T-AOC) (P<0.05), superoxide dismutase (SOD) (P<0.05) and GSH-Px levels (P<0.05) and lower malondialdehyde (MDA) levels (P<0.05) both in serum and liver versus the HF group. The study indicated that supplementation with SPO can improve lipid profiles and alleviate oxidative stress in dyslipidemic- diabetic rats induced by high-fat-diet, which showed great potential in ameliorating disorders of glucose and lipid metabolism.
J Nutr Food Sci Volume 7, Issue 4 (Suppl) ISSN: 2155-9600 JNFS, an open access journal Nutrition 2017 May 29-31, 2017
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