14Th International Nutrition & Diagnostics Conference INDC 2014
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Integrative Food, Nutrition and Metabolism Research Article ISSN: 2056-8339 14th International Nutrition & Diagnostics Conference INDC 2014 Prague, Czech Republic, September 2-5, 2014 Chairman: A. Horna Scientific Organising Committee: I. Acworth, J. Ehrenbergerová, A. Horna, P. Jandera, R. Mabrouk, M. Macka, A. Marcos, M. Šamánek, H. Tlaskalová- Hogenová, J. Xiao, Z. Zadák, T. Zima Local Organising Committee: J. Blattná, A. Horna, J. Hornová, M. Hornová, J. Vávrová, Š. Voborníková, C. Garnica Ortiz *Correspondence to: A. Horna, Institute of Nutrition and Diagnostics, RADANAL, Ltd. Okruzni 613, Pardubice, Czech Republic, Tel: +420 466 650 618; Fax: +420 467 027 020; E-mail: [email protected]; Homepage: www.indc.cz The microbiome and man theatre: established concepts application of lactic acid bacteria, E. coli and E. faecalis. Such concepts and novel prospects in medicine win increasing acceptance in clinic and practice. Our group is involved in numerous experimental and clinical studies with microbiologics Rusch V* containing viable or dead E. coli, E. faecalis and autologous vaccines Old Herborn University Foundation, Institute for Integrative in sinusitis, rhinosinusitis, bronchitis, tonsillitis, atopy, asthma, the Biology, D35745Herborn-Dill, Germany, E-mail: volker.rusch@old- irritable bowel syndrome and other disorders. A mixture of data will herborn-university.de be presented to demonstrate significant effects in experimental and clinical situations and how regulatory signals modulate host physiology A rising flood of literature published on the topic “microbiome and immune functions, including most recent findings. and man” illustrates the growth of appreciation of a new image of man and microbes as a functional unity and its role of intimate partners Colorectal cancer and gut microbiota: Probiotics and in a symbiotic system. Surprising insights into this complex ecological prebiotics in prevention? network let familiar paradigms falter and offer new routes in adaptive Tlaskalová-Hogenová H*., Klimešová K. , Zákostelská Z. , control of numerous interdependent physiological processes at multiple Kverka M., Hornová M., Vannucci L., Štěpánková R., Kozáková H., interfaces leading to an understanding of established concepts in Hudcovic T., and Rossmann P. therapeutic and preventive intervention, the promise of better targeted research and new clinical applications. Intensive studies of metazoan Institute of Microbiology, Academy of Sciences of the Czech and human physiology and its metabolic, immune and other functions Republic, Videnska 1083, Prague 4 and Nový Hrádek, Czech Republic, reveal that many functions are deeply influenced by symbiotic microbial E-mail: [email protected] communities and even brain and behaviour are affected. The gut brain Colorectal cancer is the most common malignancy of the axis links gut and brain activities in a bi-directional communication gastrointestinal tract. More frequent incidence in industrialized system utilizing neural, endocrine and immunological signaling. A new countries than in developing once could be explained by differences in comprehension of immune functions emerged and the immune system dietary habits and lifestyle. The link between diet and pathogenesis of now appears not only as a defensive system, but also as an institution colorectal cancer is likely to be due to an imbalance of gut microbiota. organising the communication between multicellular organisms and The microbiome encodes huge numbers of catabolic and anabolic microbes. As a consequence, special attention is focused upon microbes pathways in the gut enabling it to use a vast range of substrates. The as preventive and therapeutic agents in modulation of immune composition and metabolic profile of the gut microbiota is substantially and other physiological functions, as reflected in rapidly expanding and rapidly changed after major changes in diet. Balanced interaction numbers of publications on well designed and controlled studies and between commensal bacteria and the host mucosal immune system mounting evidence on clinical efficacy. Papers mainly deal with lactic is a prerequisite for homeostasis in intestinal tract. Barrier formed by acid bacteria and to a lesser extent with other microorganisms in intestinal epithelium separates intestinal microbiota from underlying probiotics or microbiologics. The medical community vividly discusses tissues, preventing bacterial infiltration and subsequent inflammation. the potential of fecal microbiota transplants and even considers Numerous chronic diseases may occur as a result of disturbance of microbes as “psychobiotics”. In Germany, a medical association intestinal microbiota composition (“dysbiosis”), impaired mucosal for microbiological therapy developed a modular system in clinical Integr Food Nutr Metab, 2014 doi: 10.15761/IFNM.1000104 Volume 1(1): 7-82 Horna A (2014) 14th International Nutrition & Diagnostics Conference INDC 2014 barrier function and changes in mechanisms regulating mucosal 2Analytics Group, German Institute of Human Nutrition Potsdam immunity. Impairment of intestinal barrier function was found Rehbruecke, Germany during development of inflammation leading to colorectal cancer 3Department of Pathology, Baylor College of Medicine, Houston development. Carcinogenesis in the gut could be driven by the Tx, USA presence of potentially harmfull microbes or by lack of protective ones, by the production of carcinogens generated by microbes, and 4Department of Gastroenterology, Charité University Hospital by the induction of inflammation and modulation of the immune Berlin, Germany system. Accumulating data suggest that ingesting of probiotics or/ 5Department of Gastrointestinal Microbiology, German Institute and prebiotics may be able to play a preventive role in the onset of of Human Nutrition Potsdam Rehbruecke, Germany, E-mail: delphine. colorectal cancer. Probiotics contribute to development and reactivity [email protected] of the mucosal immune system by promoting epithelial, dendritic, and T-cell responses. Probiotics may decrease the burden of carcinogens by Folate is one of the key vitamins involved in normal cellular influencing microbiota composition, moreover, they have stabilizing function, growth, and development. Low folate and raised homocysteine effects on the gut microbiota, potentially reducing a proinflammatory levels are risk factors for chronic inflammation. Although evidence is response. Gnotobiological approach is allowing to analyse the impact accumulating that microbially derived folates could impact the folate of defined microbes on the regulation of mucosal immunity and status of the host, their production in the gut has been so far largely carcinogenesis. Using gnotobiological methods we have shown that gut ignored. Subclinical but biochemically relevant folate deficiency microbiota promotes colitis-associated colorectal cancer by increasing occurs in 40% of inflammatory bowel diseases (IBD) patients. IBD, the exposure of gut epithelium to carcinogens. Gut microbiota and whose main manifestations are ulcerative colitis (UC) and Crohn’s its metabolic activity could be potential targets for colorectal cancer disease (CD), are the result of an altered immune homeostasis within therapy. the intestinal mucosa against the gut microbiota, leading to chronic inflammation in genetically predisposed individuals. Incidence rates The link between diet, gut microbiota and disease of IBD increased dramatically in parallel with social development but expression in rodent models of human disease etiology of the disease is unclear. Our hypothesis is that serum folate Hansen A. K* deficiency in ileal CD subjects reflects an altered folate metabolism in the gastrointestinal tract due to a host and/or a gastrointestinal Department of Veterinary Disease Biology, University of microbiota component. Furthermore, we hypothesize that altered Copenhagen, E-mail: [email protected] folate metabolism in the gastrointestinal tract contribute to microbial An increasing amount of evidence shows that a range of human dysbiosis and increased inflammation in ileal CD patients. In the diseases, such as type 1 and type 2 diabetes, colitis, obesity and first stage of this project, folate production by selected gut bacterial cancer are under impact of the diet; an impact, which often works commensals was assessed in a folate free-medium. Most strains, with in conjunction with the gut microbiota. In this context it has been the exception of Faecalibacterium prausnitzii - a species with anti- essential to perform studies in mice, in which it has been shown that inflammatory properties strongly reduced in IBD - were able to produce various dietary components within the fractions of fat, carbohydrates, both intra and extra-cellular folates. In a second stage of this project, and proteins with a documented impact on the development of disease we are defining the quantity as well as the different forms of fecal in mouse models, act through or with the gut microbiota. Sequencing folate vitamers to be obtained from 40 ileal CD patients with different techniques have allowed us a better ability to identify single bacterial serum folate status by a microbiological assay and mass spectrometry. species of importance, but it has also shown us that the more overall Furthermore, we are characterizing microbiota composition using balance between microbiota members and the time of life we meet next generation sequencing technologies. In the future, we will also these for the