Kjeldahl Distillation Unit
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Exploring Small-Scale Chemostats to Scale up Microbial Processes: 3-Hydroxypropionic Acid Production in S
Lawrence Berkeley National Laboratory Recent Work Title Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae. Permalink https://escholarship.org/uc/item/43h8866j Journal Microbial cell factories, 18(1) ISSN 1475-2859 Authors Lis, Alicia V Schneider, Konstantin Weber, Jost et al. Publication Date 2019-03-11 DOI 10.1186/s12934-019-1101-5 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Lis et al. Microb Cell Fact (2019) 18:50 https://doi.org/10.1186/s12934-019-1101-5 Microbial Cell Factories RESEARCH Open Access Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae Alicia V. Lis1, Konstantin Schneider1,2, Jost Weber1,3, Jay D. Keasling1,4,5,6,7, Michael Krogh Jensen1 and Tobias Klein1,2* Abstract Background: The physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defned changes to one single parameter at a time, which is most commonly the growth rate. The subsequent establishment of a steady state then permits constant variables enabling the acquisition of reproducible data sets for comparing microbial perfor- mance under diferent conditions. We performed physiological characterizations of a 3-hydroxypropionic acid (3-HP) producing Saccharomyces cerevisiae strain in a miniaturized and parallelized chemostat cultivation system. The physi- ological conditions under investigation were various growth rates controlled by diferent nutrient limitations (C, N, P). Based on the cultivation parameters obtained subsequent fed-batch cultivations were designed. Results: We report technical advancements of a small-scale chemostat cultivation system and its applicability for reliable strain screening under diferent physiological conditions, i.e. -
Microscope Compatible Cell-Culture Incubator
Microscope Compatible Cell-Culture Incubator BME 400 December 14th, 2016 Client: Dr. John Puccinelli, UW-Madison Dept. of Biomedical Engineering Advisor: Dr. Mitchell Tyler, UW-Madison Dept. of Biomedical Engineering Team Members: Trevor Zarecki - Team Leader Jenny Westlund - Communicator Peter Hartig - BPAG Jack McGinnity - BWIG Steve Gock - BSAC Abstract Live cell imaging experiments are difficult to perform over long periods of time on normal lab microscopes without incubation capabilities. Current commercial microscope-stage incubators are expensive, not compatible with multiple microscopes and ineffective at evenly controlling the environment. The client desires an inexpensive incubation chamber compatible with cell microscopy that is capable of maintaining desired temperature, CO2, and humidity evenly throughout the chamber. The device should not alter image quality, and should be accessible for changing media or cell culture dishes. An initial prototype has been developed that involves a small, cohesive system to regulate temperature, CO2, and humidity. Testing of the current prototype has demonstrated regulation of these three systems, through an automated feedback system capable of maintaining the system near a physiological set point. Further development of the design will ensure that it performs efficiently according to all specifications, and ultimately help bridge the gap in the market between high-cost, functional incubation systems and cheaper, less effective designs. 1 Table of Contents Introduction 3 Motivation 3 Existing -
Effective Contamination Control with CO2 Incubators
WHITE PAPER No. 30 Effective Contamination Control with CO2 Incubators Ines Kristina Hartmann¹, James Jarvis² ¹Eppendorf AG, ²Eppendorf, Inc. Executive Summary CO2 incubators provide an optimal cell growth environ- ment by maintaining a humidified atmosphere with temperature and carbon dioxide control. These conditions not only promote cell growth, but also the growth of contaminants, like bacteria, yeast, molds and other fungi. The contamination-reducing features of an incubator’s functional design and the effectiveness of its self-decon- tamination system must be considered in choosing an instrument. In this paper, we compare various strategies for preventing contamination in CO2 incubators, from the functional design of the device to self-disinfection programs. We also give some useful tips to prevent contamination when using CO2 incubators. Introduction Sources of contamination How do contaminants get into a CO2 incubator? Contamination is a major cause of frustration when culturing The CO2 incubator may become an indirect source of con- cells. There are many sources of contamination, either direct tamination. Unlike a biological safety cabinet, an incubator or indirect. Direct sources are contaminated reagents, media cannot prevent the influx of airborne contaminants, as the or seed culture. Media and reagents from reputable suppliers door must be opened during routine use. The incubator are rarely delivered contaminated nowadays. New cell lines chamber can also be contaminated by carelessness in aseptic can introduce contaminants into the lab, especially when techniques, and unnoticed splashes from cell culture vessels. they are given from lab to lab. They should be quarantined Some CO2 incubators use a HEPA filter to remove microor- before culturing with the rest of the cells. -
Microbiology Laboratory Exercises Third Edition 2020
MICROBIOLOGY Laboratory Exercises Third Edition Keddis & Rauschenbach 2020 Photo Credits (in order of contribution): Diane Davis, Ines Rauschenbach & Ramaydalis Keddis Acknowledgements: Many thanks to those in the Department of Biochemistry and Microbiology, Rutgers University, who have through the years inspired our enthusiasm for the science and teaching of microbiology, with special thanks to Diane Davis, Douglas Eveleigh and Max Häggblom. Safety: The experiments included in this manual have been deemed safe by the authors when all necessary safety precautions are met. The authors recommend maintaining biosafety level 2 in the laboratory setting and using risk level 1 organisms for all exercises. License: This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International License Microbiology Laboratory Exercises Third Edition 2020 Ramaydalis Keddis, Ph.D. Ines Rauschenbach, Ph.D. Department of Biochemistry and Microbiology Rutgers, The State University of New Jersey CONTENTS PAGE Introduction Schedule ii Best Laboratory Practices Iii Working in a Microbiology Laboratory iv Exercises Preparation of a Culture Medium 1 Culturing and Handling Microorganisms 3 Isolation of a Pure Culture 5 Counting Bacterial Populations 8 Controlling Microorganisms 10 Disinfectants 10 Antimicrobial Agents: Susceptibility Testing 12 Hand Washing 14 The Lethal Effects of Ultraviolet Light 15 Selection of Fungi from Air 17 Microscopy 21 Morphology and Staining of Bacteria 26 Microbial Metabolism 30 Enzyme Assay 32 Metabolic -
Standard Operating Procedures
Standard Operating Procedures 1 Standard Operating Procedures OVERVIEW In the following laboratory exercises you will be introduced to some of the glassware and tech- niques used by chemists to isolate components from natural or synthetic mixtures and to purify the individual compounds and characterize them by determining some of their physical proper- ties. While working collaboratively with your group members you will become acquainted with: a) Volumetric glassware b) Liquid-liquid extraction apparatus c) Distillation apparatus OBJECTIVES After finishing these sessions and reporting your results to your mentor, you should be able to: • Prepare solutions of exact concentrations • Separate liquid-liquid mixtures • Purify compounds by recrystallization • Separate mixtures by simple and fractional distillation 2 EXPERIMENT 1 Glassware Calibration, Primary and Secondary Standards, and Manual Titrations PART 1. Volumetric Glassware Calibration Volumetric glassware is used to either contain or deliver liquids at a specified temperature. Glassware manufacturers indicate this by inscribing on the volumetric ware the initials TC (to contain) or TD (to deliver) along with the calibration temperature, which is usually 20°C1. Volumetric glassware must be scrupulously clean before use. The presence of streaks or droplets is an indication of the presence of a grease film. To eliminate grease from glassware, scrub with detergent solution, rinse with tap water, and finally rinse with a small portion of distilled water. Volumetric flasks (TC) A volumetric flask has a large round bottom with only one graduation mark positioned on the long narrow neck. Graduation Mark Stopper The position of the mark facilitates the accurate and precise reading of the meniscus. If the flask is used to prepare a solution starting with a solid compound, add small amounts of sol- vent until the entire solid dissolves. -
SC-300 Pro Kit Manual 1 Version 2.3D Materials Provided in the Kit
Vinmetrica SC-300 Pro Kit™ User Manual Vinmetrica SC-300 Pro Kit is a simple and robust device that provides high accuracy in determination of sulfite (SO2), pH and titratable acidity (TA) levels in wines, ciders, and other liquids. These are essential parameters to control in the effort to make high quality wines. The Pro kit includes lab accessories for the SC-300 Analyzer. Table of Contents: Materials Provided in the Kit............................................................................................................... 2 Things you will need............................................................................................................................ 2 Why Test for SO2, pH and TA?............................................................................................................ 3 Theory of Operation............................................................................................................................. 4 Setup.................................................................................................................................................... 5 Setting up the SC-300 for the first time................................................................................... 5 Assembling the Pro Kit Equipment......................................................................................... 6 Burette Maintenance................................................................................................................ 8 Burette Reading ...................................................................................................................... -
Technical Guidance for Microbiology Incubators
DRAFT – FOR DISCUSSION PURPOSES ONLY DEPARTMENT OF ENVIRONMENTAL PROTECTION Laboratory Accreditation Program Document Number: # Title: Microbiology Incubator Guidance Effective Date: Date Authority: 27 Pa.C.S. §§ 4101 – 4113 (relating to environmental laboratory accreditation), Environmental Laboratory Accreditation Regulations, 25 Pa. Code Chapter 252 Policy: It is the policy of the Department of Environmental Protection (DEP) to provide laboratory management personnel with the information necessary to either obtain or maintain accreditation to perform and report environmental analyses in Pennsylvania. Purpose: This technical guidance will provide laboratory management with the tools to develop procedures for maintenance and verification of incubation units used for microbiological testing that meet the requirements for laboratory accreditation. Applicability: This guidance will apply to all laboratories desiring to obtain and/or maintain accreditation under 25 Pa. Code Chapter 252. Disclaimer: The policies and procedures outlined in this guidance document are intended to supplement existing requirements. Nothing in the policies or procedures will affect regulatory requirements. The policies and procedures herein are not an adjudication or a regulation. There is no intent on the part of the Department to give these rules that weight or deference. This document establishes the framework within which DEP will exercise its administrative discretion in the future. DEP reserves the discretion to deviate from this policy statement if circumstances -
General Chemistry Laboratory I Manual
GENERAL CHEMISTRY LABORATORY I MANUAL Fall Semester Contents Laboratory Equipments .............................................................................................................................. i Experiment 1 Measurements and Density .............................................................................................. 10 Experiment 2 The Stoichiometry of a Reaction ..................................................................................... 31 Experiment 3 Titration of Acids and Bases ............................................................................................ 10 Experiment 4 Oxidation – Reduction Titration ..................................................................................... 49 Experiment 5 Quantitative Analysis Based on Gas Properties ............................................................ 57 Experiment 6 Thermochemistry: The Heat of Reaction ....................................................................... 67 Experiment 7 Group I: The Soluble Group ........................................................................................... 79 Experiment 8 Gravimetric Analysis ........................................................................................................ 84 Scores of the General Chemistry Laboratory I Experiments ............................................................... 93 LABORATORY EQUIPMENTS BEAKER (BEHER) Beakers are containers which can be used for carrying out reactions, heating solutions, and for water baths. They are for -
Microlab® STAR™
Microlab ® STAR™ Microlab ® STAR ™ AUTOMATED WORKFLOW SOLUTIONS CENTERED AROUND YOUR ASSAY The STAR combines Hamilton's patented pipetting technology including precise lock-and-key tip attachment, unrivaled liquid level detection, and comprehensive volume ranges to create flexible liquid handling workstations. Available in three base platform sizes, the STAR portfolio incorporates countless options to automate your workflows. Hamilton Robotics has also partnered with top leaders in the biotechnology industry to provide Standard Solutions based on commonly automated applications. Offering ready-to-start protocols for a variety of applications such as NGS, ELISA, and forensic assays, our Standard Solutions provide a faster way to automate your processes. 2 1 PATENTED TECHNOLOGY The STAR utilizes Hamilton’s proprietary Compressed O-Ring Expansion (CO-RE®) technology. CO-RE minimizes the production of aerosols and allows disposable tips or washable, steel needles to be used on channels in the same run. 2 MULTI-FUNCTIONAL ARM Our technology offers high pipetting accuracy and precision, from sub-microliter to large volumes, using Independent Channels and/or the Multi-Probe Head (MPH). Labware transportation is possible with the iSWAP® or CO-RE Grippers. The STAR can incorporate a camera, tube transportation, and other channel tools on a single arm. Comprehensive pipetting range: ■■■0.5 μL to 1 mL using the 1 mL Independent Channel ■■■50 μL to 5 mL using the 5 mL Independent Channel ■■■1 μL to 1 mL using the CO-RE 96 MPH ■■■0.1 μL to 50 μL using the CO-RE 384 MPH 3 FLEXIBLE SETUP The high-capacity deck is customized specific to your workflow, accommodating a wide range of labware and automated devices that can easily be exchanged to support multiple assays on one platform. -
Thermo Scientific Solutions Automated Liquid Handling, Detection Model Cat
THERMO SCIENTIFIC AUTOMATED LIQUID HANDLING, DETECTION AND SAMPLE PURIFICATION SOLUTIONS Versette™ RapidStak™ Also available: 96-and 384-channel Automated Microplate • A complete range of 96- and 384-well solid and strip microplates. Automated Liquid Handler Stacker • A complete line of sample storage tubes and equipment. • Total volume range 0.5-300 µl • Works seamlessly with • A complete range of Thermo Scientific manual and electronic • 96- and 384-channel interchangeable the entire Multidrop line of single and multichannel pipettes and tips. pipetting heads dispensers • Laboratory automation solutions for microplate instrument systems. • Compatible with D.A.R.T.sTM tips with • Schedule and automate any unique sealing properties two instruments with the • 6-position stage with compact dual level Thermo ScientificTM PolaraTM RS deck structure software For additional information contact your ™ • User-friendly programming options • Capacity from 30 to 150 plates Thermo Fisher Scientific sales representative or visit: with onboard touch screen or Thermo • Quick and easy setup www.thermoscientific.com/kingfisherinfo ScientificTM ControlMateTM PC software Model Cat. No. www.thermoscientific.com/versette RapidStak F01350 www.thermoscientific.com/multidrop RapidStak 2x F01351 Model Cat. No. www.thermoscientific.com/platereaders 30-Plate Stak Versette F01436 www.thermoscientific.com/wellwash Reference Guide Versette base unit 650-01-BS 50-Plate Stak F01437 www.thermoscientifc.com/ELISAsolutions 96- and 384-channel pipetting module for use with 650-02-NTC Polara RS Inquire 96- and 384-channel pipetting head 6-position stage 650-03-SPS Versette Pipetting Heads Orbitor RS™ 96-channel air displacement pipetting head. 650-06-9630 Volume 0.5-30 µl Automated Microplate Mover • Dedicated plate mover providing 96-channel air displacement pipetting head. -
2020 NGSS High School Chemistry Supply List
Recommended Minimum Core Inventory for NGSS High School Chemistry, Class of 32 Students Qty. Per Catalog Equipment / Supplies FREY Catalog # Total Classroom Unit $ SAFETY EQUIPMENT 1 Acid Corrosive Storage Cabinet 528445 $602.09 $602.09 1 Corrosive Storage Cabinet 528446 $1,009.95 $1,009.95 1 Chemical Spill Kit 1491078 $146.29 $146.29 Chemical Storage Reference - Ask your Frey 1 Chemical Storage Reference Scientific Representative how to safely store your chemicals with our Color Code System. 1 Emergency Shower and Eyewash 572443 $3,135.95 $2,983.95 1 Gravit-Eye Portable Eye Wash Station, 16 gallon 1320143 $333.39 $333.39 1 Fire Blanket w/ Hanging Pouch, 6' L x 5' W 1488340 $149.99 $149.99 1 Dry Chemical Fire Extinguisher 1471240 $153.79 $153.79 1 First Aid Station for 25 people 1451983 $70.09 $70.09 1 Flammables Storage Cabinet, 23-1/4" x 35" x 12 gallon, yellow 601016 $960.95 $960.95 1 Fume Hood Labconco Basic 47 526701 $10,081.49 $10,081.49 32 Indirect Vent Safety Goggles 577927 $3.89 $124.48 1 Goggle Sanitizer Cabinet 1574050 $911.99 $911.99 32 Rubberized Aprons, 27" x 42" 589239 $8.39 $268.48 EQUIPMENT / SUPPLIES 1 Balance, Ohaus Pioneer Precision, 0.001 mg, 160g capacity 2001994 $1,254.69 $1,254.69 5 Balance, Ohaus Navigator Electronic , .01g, 510g capacity 2012765 $328.79 $1,643.95 3 Beakers, 50 mL, Pyrex Vista Low Form Borosilicate, pk/12 529623 $35.09 $105.27 3 Beakers, 100 mL, Pyrex Vista Low Form Borosilicate, pk/12 529624 $35.09 $105.27 2 Beakers, 250 ml, Pyrex Vista Low Form Borosilicate pk/12 529626 $37.39 $74.78 2 Beakers, -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout