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CURRICULUM VITAE NAME: Patricia Lynn Opresko BUSINESS ADDRESS: University of Pittsburgh Graduate School of Public Health Department of Environmental and UPMC Hillman Cancer Center 5117 Centre Avenue, Suite 2.6a Pittsburgh, PA15213-1863 Phone: 412-623-7764 Fax: 412-623-7761 E-mail: [email protected] EDUCATION AND TRAINING Undergraduate 1990 - 1994 DeSales University B.S., 1994 Chemistry and Center Valley, PA Biology Graduate 1994 - 2000 Pennsylvania State Ph.D., 2000 Biochemistry and University, College of Molecular Biology Medicine, Hershey, PA Post-Graduate 3/2000 - 5/2000 Pennsylvania State Postdoctoral Dr. Kristin Eckert, University, College of Fellow Mutagenesis and Medicine, Jake Gittlen Cancer etiology Cancer Research Institute Hershey, PA 2000-2005 National Institute on IRTA Postdoctoral Dr. Vilhelm Bohr Aging, National Fellow Molecular Institutes of Health, Gerontology and Baltimore, MD DNA Repair 1 APPOINTMENTS AND POSITIONS Academic 8/1/2018 – Co-leader Genome Stability Program, UPMC present Hillman Cancer Center 5/1/2018- Tenured Professor Pharmacology and Chemical Biology, present School of Medicine, University of Pittsburgh, Pittsburgh, PA 2/1/2018- Tenured Professor Environmental and Occupational Health, present Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 2014 – Tenured Associate Environmental and Occupational Health, 1/31/2018 Professor Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 2005 - 2014 Assistant Professor Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 3/2000-5/2000 Postdoctoral Fellow Pennsylvania State University, College of Medicine, Jake Gittlen Cancer Research Institute Hershey, PA Non-Academic 2000 - 2005 IRTA Postdoctoral Laboratory of Molecular Gerontology, Fellow National Institute on Aging, National Institutes of Health, Baltimore, MD MEMBERSHIP IN PROFESSIONAL AND SCIENTIFIC SOCIETIES 1999 - prsent American Association for Cancer Research 2005 - present Environmental Mutagenesis and Genomics Society 2006 - present University of Pittsburgh Cancer Institute. Molecular and Cellular Cancer Biology Program 2010-present Invited member of the Center for Nucleic Acids Science and Technology, Carnegie Mellon University 2 HONORS 1991 Freshman Chemistry Achievement Award, CRC Press 1993 Delta Delta Chapter of Delta Epsilon Sigmas, National Catholic Collegiate Honor Society 1994 American Chemical Society Award, Lehigh Valley Chapter of ACS 1999 Special Conference Travel Grant Award, American Association for Cancer Research 1998-1999 Mentored Investigator Award, The Four Diamonds Fund of the Milton S. Hershey 2005 Travel Award, Environmental Mutagen Society 2006 Selected to present at the Senior Vice Chancellor's Research Seminar Series, University of Pittsburgh 2006 Ellison Medical Foundation New Scholar in Aging Research 2006 Outstanding New Environmental Scientist (ONES), NIEHS 2008, 2010 Annual Meeting of the Environmental Mutagen Society, Session Co-chair 2009 Annual International Conference on Environmental Mutagens, Session Co- chair 2011 2011 FASEB meeting on Helicases and NTP-Driven Nucleic Acid Motors, Session Chair 2012 2012 Gordon Conference on DNA damage, mutation and cancer; Session Chair 2015 Invited to give the keynote lecture at the Pennsylvania State University School of Medicine Graduate Student Research Forum. 3 2016 Elected as a Councilor of the Environmental Mutagenesis and Genomics Society for the 2017-2020 term. 2016 Research featured in Pittsburgh Post Gazette and Pittsburgh Tribune Review 2017 NSMB article was selected for NIEHS Papers of the Month in January 2017 2017 Gordon Conference on Mammalian DNA Repair; Session Chair 2017 Research was highlighted in PittMed Magazine: Summer 2017 2018 Invited Public Lecture at the annual meeting for the German DNA Repair Society, Karlsruhe, Germany. 2018 Glenn Award for Research in Biological Mechanisms of Aging 2019 Elected next Vice Chair and Chair of the Gordon Conference on Mammalian DNA Repair PROFESSIONAL ACTIVITIES 1. Teaching a. Courses Taught Years Course Number: Title Hours of Role in course Taught Lecture, credits Primary/Coordinator Average Enrollment Spring EOH 3210: 1.5 hrs, 12 Lecturer 2015, 17, Pathophysiology and graduate students 19 Environmental Disease 2013-15 MLB 1: Methods and 2 hrs, 6 Tsinghua Facilitator/ Dr. Peter Logic in Biomedicine Students Drain Course Director Spring EOH 3305, MSMPHL 1.5 hrs, 3 credits, Co-director with Drs. biennial 3330 and MSBMG 3530: 5 students Bennett Van Houten 2006 -18 Genome instability and 3 lectures and Chris Bakkenist human disease 4 Years Course Number: Title Hours of Role in course Taught Lecture, credits Primary/Coordinator Average Enrollment Fall EOH 2310: Molecular 3 hrs, 3 credits Co-director with Dr. biennial Fundamentals 6 students Peter Di 2010-12 2015-18 Summer EPIDEM 2980 1 hr, 1 credit, Lecturer biennial Biology and Physiology of 15 students Director: Dr. Anne 2008-16 Aging 1 lecture Newman Fall HUGEN 2031 2 hr, 2 credits Lecturer 2010-11 Introduction to Human 21 students Director: Dr. Suzanne 2014-18 Genetics Gollin Fall EOH 2175: General 1.5 hr, 3 credits Lecturer 2007-18 Toxicology 2 lectures Director: Dr. James 15 students Fabisiak Fall MSELCT 5130-1020. 1.5 hr Co-director with Dr. 2011 M.S.T.P. Research Basis of 16 sessions Bennett van Houten Medical Knowledge 11-16 students SOM, 2nd year students Fall/Spring MSLECT 5130-1020. 1.5 hr Co-director with 2006-10 M.S.T.P. Research Basis of 12 sessions Dr. Richard Medical Knowledge 11-16 students Steinman SOM, 1st year students Fall EOH 2310: Molecular 1 hr, 3 credits Lecturer 2005-08 Fundamentals 7 Lectures Directors: Dr. Paul Reynolds Dr. Sy Garte b. Other Teaching (lectures, tutorials and continuing education courses) Date(s) Type of Teaching Title Fall 2006 1hr lecture "Basic orientation to 'bench' Biomarkers of biomarkers of age with an emphasis on Aging Workshop telomeres" to faculty, postdoctoral November 7, 2006 fellows and graduate students. The Pittsburgh Mind-Body Center 5 Date(s) Type of Teaching Title Fall 2006 Preceptor for article "The aflatoxin B(1) EOH 2176: formamidopyrimidine adduct plays a Principles of major role in causing the types of Toxicology mutations observed in human Conference hepatocellular carcinoma." Smela et al. EOH Graduate 2002 PNAS. Program Fall 2006 Preceptor for article "Xeroderma EOH 2176: pigmentosum variant (XP-V) correcting Principles of protein from HeLa cells has a thymine Toxicology dimer bypass DNA polymerase activity". Conference Matsutani et al. 1999 EMBO J EOH Graduate Program Spring 2006 Lecture on experience with the peer EOH 2109: review for a publication “POT1 Graduate Program Stimulates RecQ Helicases WRN and in Molecular BLM to Unwind Telomeric DNA Toxicology Journal Substrates” Opresko et al., 2005, Journal Club Biological Chemistry. Fall 2005 Mentor/preceptor for student EOH 2311: Mol presentation of the article “DNA Fundamentals Helicase Srs2 disrupts the Rad51 Conference. presynaptic filament” Krejci 2003 EOH Graduate Nature. Program Fall 2005 Mentor for Aaron Secrest and Oni Obi Journal Club 2nd for their presentation of the article year MD/PhD “Defective Telomere Lagging Strand students Synthesis in Cells Lacking WRN Helicase Activity” Crabbe et al, 2004, Science. Fall/Spring Tutored Medical Students for the Dept. Biochemistry, 1996-97 Biochemistry course Pennsylvania State University School of Medicine 1993 – 94 Private high school Chemistry tutor Undergraduate Chemistry, Center Valley, PA 1991 – 94 Peer tutor in under-graduate Biology Biology Dept, DeSales University, Center Valley, PA c. Major Advisor for Graduate Student Essays, Theses, and Dissertations 6 Name of Degree Type of Document and Title Notes Student Awarded, Year Gerald Nora PhD, MBSB Thesis, Processing of Alternative Attending April 2010 DNA Structures in the Human physician at Telomere Columbia- St. Mary’s, Shorewood, WI Rama Rao Ph.D., HuGen Werner Syndrome protein and Postdoctoral Damerla April 2011 telomeric DNA replication fellow at Memorial Sloan Kettering Cancer Center Hannah Pope- Ph.D., EOH Cellular Defense against Telomere Student is Varsalona August 2014 Dysfunction Induced by Exogenous now a Genotoxicants Toxicologist at EPA Dhvani Ph.D., EOH Nucleotide Excision Repair at Student is Mukesh Parikh December Telomeres now a 2014 Toxicologist at the U.S. Green Building Council, D.C. Samantha Ph.D., IDM TBA Sanford Expected 2021 Samuel Ph.D., MBSB TBA Johnson Expected 2022 d. Service on Masters or Doctoral Committees Dates Name of Degree Title of Dissertation/Essay Served Student Awarded 6/2019 Kirill PhD, TBA Lavrenyuk Molecular Biophysics and Structural Biology Program 7 Dates Name of Degree Title of Dissertation/Essay Served Student Awarded 6/2019 Daniel PhD, TBA Whitefiled Biomedical Engineering, CMU 9/2018 Thong The PhD, TBA Luong Pharmacology and Chemical Biology 4/2018 Xiaoshuang MPH, The association between telomere length Xun Epidemiology and risk of breast cancer in Singapore Chinese Health Study 4/2018 Meiyuzhen MPH, The role of telomere length in the risk of Qi Epidemiology colorectal cancer incidences: A cohort study from the Singapore Chinese Health Study 10/2017- My Song PhD, TBA present Hoang Pharmacology and Chemical Biology 10/2016 - Emily PhD, TBA present Beckwitt Molecular Structural Biology and Biophysics 4/21/2016 Stanley PhD, Recognition of Guanine Quadruplexes thesis Oyaghire Chemistry, by PNA and Gamma-PNA Oligomers defense Carnegie Mellon
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    UNIVERSITY OF CINCINNATI _____________ , 20 _____ I,______________________________________________, hereby submit this as part of the requirements for the degree of: ________________________________________________ in: ________________________________________________ It is entitled: ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ Approved by: ________________________ ________________________ ________________________ ________________________ ________________________ BLM is a Suppressor of DNA Recombination A dissertation submitted to the Division of Research and Advanced Studies Of the University of Cincinnati In partial fulfillment of the Requirements for the degree of Doctorate of Philosophy (Ph.D.) In the Department of Molecular Genetics, Microbiology, and Biochemistry Of the College of Arts and Sciences 2002 by Joel E. Straughen B.S., The Ohio State University, 1985 M.D., University of Cincinnati, 2002 Committee Chair: Joanna Groden, Ph.D. i ABSTRACT Bloom’s syndrome (BS) is a rare, recessive chromosome breakage disorder characterized by small stature, sun sensitivity, facial erythema, immunodeficiency, female subfertility, male infertility, and a predisposition to a variety of cancers. When this body of work was started, the gene for Bloom’s syndrome (BLM)hadyettobe identified. This work presents characterization of the genomic region at BLM and the identification of BLM. With the cloning
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