Peptide Summary Report (Submitted from PROJ13988 by Mascot

Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

User : Matt F Email : Search title : Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08 MS data file : \\Apaf-hpv-file\User_Shared\MFitzhenry\2012 Reports\PROJ13988_LingZhu(SSI)\A9.mgf Database : SwissProt 2012 (536489 sequences; 190389898 residues) Taxonomy : Homo sapiens (human) (20312 sequences) Timestamp : 3 Jul 2012 at 05:04:17 GMT Protein hits : K2C1_HUMAN Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 GDE_HUMAN Glycogen debranching enzyme OS=Homo sapiens GN=AGL PE=1 SV=3 K1C9_HUMAN Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 K1C10_HUMAN Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 K22E_HUMAN Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 EEA1_HUMAN Early endosome antigen 1 OS=Homo sapiens GN=EEA1 PE=1 SV=2 UGGG1_HUMAN UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens GN=UGGT1 PE=1 SV=3 A2MG_HUMAN Alpha-2-macroglobulin OS=Homo sapiens GN=A2M PE=1 SV=3 ALBU_HUMAN Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2 K2C6B_HUMAN Keratin, type II cytoskeletal 6B OS=Homo sapiens GN=KRT6B PE=1 SV=5 SPTA2_HUMAN Spectrin alpha chain, brain OS=Homo sapiens GN=SPTAN1 PE=1 SV=3 K2C5_HUMAN Keratin, type II cytoskeletal 5 OS=Homo sapiens GN=KRT5 PE=1 SV=3 K1H2_HUMAN Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3 K1C24_HUMAN Keratin, type I cytoskeletal 24 OS=Homo sapiens GN=KRT24 PE=1 SV=1 AP1B1_HUMAN AP-1 complex subunit beta-1 OS=Homo sapiens GN=AP1B1 PE=1 SV=2 K22O_HUMAN Keratin, type II cytoskeletal 2 oral OS=Homo sapiens GN=KRT76 PE=1 SV=2 PUR2_HUMAN Trifunctional purine biosynthetic protein adenosine-3 OS=Homo sapiens GN=GART PE=1 SV=1 PLCB1_HUMAN 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Homo sapiens GN=PLCB1 PE=1 SV=1 RPA1_HUMAN DNA-directed RNA polymerase I subunit RPA1 OS=Homo sapiens GN=POLR1A PE=1 SV=2

SwissProt Decoy False discovery rate Peptide matches above identity threshold 66 5 7.58 % Peptide matches above homology or identity threshold 77 5 6.49 %

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 30 indicate identity or extensive homology (p<0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Downloaded from iovs.arvojournals.org on 10/02/2021 1 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

Peptide Summary Report

Help Significance threshold p< Max. number of hits Show Percolator scores Standard scoring MudPIT scoring Ions score or expect cut-off Show sub-sets Show pop-ups Suppress pop-ups Sort unassigned Require bold red

Error tolerant

1. K2C1_HUMAN Mass: 65999 Score: 401 Matches: 17(10) Sequences: 16(9) emPAI: 0.93 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 162 437.7582 873.5018 873.4920 11.2 0 42 0.005 1 U R.SLVNLGGSK.S 286 487.2764 972.5383 972.5240 14.7 0 48 0.001 1 K.IEISELNR.V 301 500.2318 998.4490 998.4379 11.2 0 4 23 1 U K.DVDGAYMTK.V 324 517.2700 1032.5255 1032.5087 16.2 0 30 0.072 1 U R.TLLEGEESR.M 342 533.2775 1064.5405 1064.5138 25.1 0 29 0.071 1 U K.AQYEDIAQK.S 396 590.3155 1178.6164 1178.5931 19.7 0 69 8.5e-006 1 K.YEELQITAGR.H 451 633.3401 1264.6657 1264.6299 28.3 0 70 6.2e-006 1 U R.TNAENEFVTIK.K 459 639.3636 1276.7126 1276.7027 7.81 0 43 0.0033 1 U K.LALDLEIATYR.T 491 651.8632 1301.7118 1301.7078 3.09 0 (63) 2.7e-005 1 U R.SLDLDSIIAEVK.A 492 651.8704 1301.7263 1301.7078 14.2 0 77 1.2e-006 1 U R.SLDLDSIIAEVK.A 553 692.3265 1382.6385 1382.6830 -32.18 0 2 36 2 U K.SLNNQFASFIDK.V 558 465.2548 1392.7425 1392.7249 12.7 1 57 0.00011 1 U R.TNAENEFVTIKK.D 585 738.3873 1474.7601 1474.7416 12.5 0 85 1.7e-007 1 U K.WELLQQVDTSTR.T 586 738.4002 1474.7858 1474.7780 5.30 0 94 2e-008 1 R.FLEQQNQVLQTK.W 601 513.9425 1538.8056 1538.7762 19.1 1 24 0.2 1 U R.LLRDYQELMNTK.L + Oxidation (M) 619 546.9683 1637.8832 1637.8525 18.7 1 19 0.71 1 U K.SLNNQFASFIDKVR.F 737 673.3141 2016.9206 2016.8768 21.7 1 22 0.26 1 U R.LDSELKNMQDMVEDYR.N + 2 Oxidation (M)

2. GDE_HUMAN Mass: 174652 Score: 301 Matches: 17(8) Sequences: 17(8) emPAI: 0.28 Glycogen debranching enzyme OS=Homo sapiens GN=AGL PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 29 407.2449 812.4752 812.4756 -0.45 0 27 0.13 1 U R.LGPTLQGK.A 126 429.7784 857.5422 857.5334 10.2 0 19 1 1 U R.LGISSLIR.E 270 478.2676 954.5207 954.4117 114 0 10 5.5 2 U R.FSCDVAEGK.Y 274 481.2847 960.5549 960.5392 16.3 0 29 0.09 1 U K.IPFASLASR.L 394 587.8311 1173.6476 1173.6241 20.0 0 69 9e-006 1 U R.DGSAVEIVGLSK.S 441 623.8029 1245.5913 1245.5626 23.1 0 32 0.038 1 U R.SLDWENPTER.E 465 641.8395 1281.6644 1281.6353 22.6 0 75 1.9e-006 1 U R.LEQGYELQFR.L

Downloaded from iovs.arvojournals.org on 10/02/2021 2 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

531 673.3878 1344.7610 1344.7402 15.5 0 74 2.4e-006 1 U K.SGGGYIVVDPILR.V 551 691.3536 1380.6926 1380.6674 18.3 0 28 0.091 1 U R.NFAEPGSEVYLR.R 560 700.3776 1398.7407 1398.7354 3.74 0 54 0.00021 1 U K.SGSLAVDNADPILK.I 579 726.3799 1450.7452 1450.7092 24.8 0 24 0.23 1 U K.YTWNDVGQLVEK.L 580 729.8389 1457.6632 1457.6245 26.5 0 63 2.8e-005 1 U R.SGDWMIDYVSNR.L + Oxidation (M) 590 496.9575 1487.8507 1487.8269 16.0 1 28 0.092 1 U R.ILLLNEMEKLEK.T + Oxidation (M) 593 501.9450 1502.8133 1502.7954 11.9 0 27 0.1 1 U R.HSPSIHQSVVAVSR.T 599 513.2671 1536.7795 1536.7685 7.19 1 5 16 4 U R.NFAEPGSEVYLRR.E 731 653.0110 1956.0112 1955.9589 26.8 0 44 0.0021 1 U R.GYDELVPHQISVVSEER.F 734 988.4999 1974.9852 1974.9476 19.1 0 65 1.5e-005 1 U K.AVTVYTNYPFPGETFNR.E

3. K1C9_HUMAN Mass: 62027 Score: 277 Matches: 13(9) Sequences: 11(8) emPAI: 0.69 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 200 449.2196 896.4246 896.4062 20.5 0 41 0.0047 1 U R.MTLDDFR.I 226 457.2132 912.4118 912.4011 11.7 0 (33) 0.027 1 U R.MTLDDFR.I + Oxidation (M) 340 530.7877 1059.5609 1059.5560 4.62 0 51 0.00055 1 U K.TLLDIDNTR.M 341 533.2644 1064.5143 1064.4920 20.9 0 24 0.23 1 U K.STMQELNSR.L 348 537.7765 1073.5385 1073.5142 22.7 0 38 0.011 1 U R.QFSSSYLSR.S 354 541.7592 1081.5038 1081.4862 16.3 0 35 0.019 1 U K.FEMEQNLR.Q + Oxidation (M) 427 616.8168 1231.6190 1231.5906 23.1 0 105 2.2e-009 1 U R.SGGGGGGGLGSGGSIR.S 429 618.2744 1234.5342 1234.5215 10.3 0 45 0.0018 1 U R.FSSSSGYGGGSSR.V 534 450.9115 1349.7126 1349.6800 24.2 1 3 31 6 U K.IGLGGRGGSGGSYGR.G 685 896.3719 1790.7293 1790.7205 4.90 0 89 6.8e-008 1 U R.GGSGGSYGGGGSGGGYGGGSGSR.G 713 617.9898 1850.9474 1850.9196 15.0 1 (7) 9.8 2 U K.TLNDMRQEYEQLIAK.N 717 623.3186 1866.9341 1866.9145 10.5 1 22 0.26 1 U K.TLNDMRQEYEQLIAK.N + Oxidation (M) 830 837.3935 2509.1585 2509.1245 13.6 0 50 0.00038 1 U K.EIETYHNLLEGGQEDFESSGAGK.I

4. K1C10_HUMAN Mass: 58792 Score: 266 Matches: 13(9) Sequences: 13(9) emPAI: 0.74 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 19 404.2074 806.4002 806.3923 9.91 0 41 0.0055 1 R.LAADDFR.L 297 497.2528 992.4911 992.4927 -1.63 0 39 0.009 1 U K.YENEVALR.Q 322 516.3108 1030.6071 1030.5910 15.6 0 21 0.49 1 U R.VLDELTLTK.A 358 545.7764 1089.5383 1089.5237 13.4 0 35 0.021 1 K.VTMQNLNDR.L 367 555.2456 1108.4766 1108.4825 -5.34 0 37 0.011 1 U K.DAEAWFNEK.S 389 583.3006 1164.5866 1164.5775 7.83 0 49 0.00069 1 U R.LENEIQTYR.S 428 617.8534 1233.6922 1233.6717 16.6 1 30 0.055 1 U R.LKYENEVALR.Q 450 631.8101 1261.6057 1261.5899 12.6 0 62 3.5e-005 1 U R.SLLEGEGSSGGGGR.G 545 453.2509 1356.7307 1356.7110 14.6 1 14 2.4 1 U R.QSVEADINGLRR.V 550 691.3312 1380.6478 1380.6408 5.07 0 84 2.1e-007 1 U R.ALEESNYELEGK.I

Downloaded from iovs.arvojournals.org on 10/02/2021 3 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

575 478.9299 1433.7679 1433.7626 3.67 1 25 0.16 1 U K.IRLENEIQTYR.S 647 854.4003 1706.7860 1706.7649 12.4 0 108 8.8e-010 1 U K.GSLGGGFSSGGFSGGSFSR.G 856 968.8161 2903.4265 2903.3753 17.6 0 23 0.21 1 U R.NVSTGDVNVEMNAAPGVDLTQLLNNMR.S + 2 Oxidation (M)

5. K22E_HUMAN Mass: 65393 Score: 226 Matches: 12(7) Sequences: 12(7) emPAI: 0.47 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 64 416.2538 830.4931 830.4862 8.28 0 14 3 1 U R.SLVGLGGTK.S 286 487.2764 972.5383 972.5240 14.7 0 48 0.001 1 K.IEISELNR.V 326 519.2794 1036.5443 1036.5189 24.5 0 31 0.044 1 U R.YLDGLTAER.T 328 521.2928 1040.5710 1040.5502 19.9 0 21 0.44 1 U K.VDPEIQNVK.A 348 537.7765 1073.5385 1073.4812 53.4 0 16 1.8 3 U R.STSSFSCLSR.H 366 554.2852 1106.5558 1106.5356 18.3 0 33 0.03 1 K.AQYEEIAQR.S 408 604.8200 1207.6254 1207.6085 14.0 0 9 8.5 1 U R.TAAENDFVTLK.K 445 627.8215 1253.6284 1253.6001 22.6 0 65 1.7e-005 1 U R.GFSSGSAVVSGGSR.R 516 665.3767 1328.7388 1328.7187 15.1 0 58 0.00011 1 R.NLDLDSIIAEVK.A 523 446.2489 1335.7249 1335.7034 16.1 1 25 0.18 1 U R.TAAENDFVTLKK.D 582 730.9178 1459.8211 1459.7922 19.8 0 63 3.2e-005 1 U K.VDLLNQEIEFLK.V 586 738.4002 1474.7858 1474.7780 5.30 0 94 2e-008 1 R.FLEQQNQVLQTK.W

6. EEA1_HUMAN Mass: 162367 Score: 195 Matches: 9(7) Sequences: 9(7) emPAI: 0.22 Early endosome antigen 1 OS=Homo sapiens GN=EEA1 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 329 522.3171 1042.6196 1042.6022 16.7 0 20 0.65 1 U K.VSQLDIQIK.A 373 564.8232 1127.6318 1127.6186 11.7 0 35 0.02 1 U K.QIEALQGELK.I 377 573.3109 1144.6073 1144.6339 -23.24 0 30 0.062 1 U K.TELLLSAEAAK.T 385 580.8378 1159.6611 1159.6449 14.0 0 62 4.3e-005 1 U R.IQTTVTELQK.V 391 585.3587 1168.7029 1168.6815 18.3 0 53 0.00029 1 U K.IQNLEALLQK.S 413 609.3444 1216.6742 1216.6663 6.47 0 44 0.0022 1 U K.ITTQLDQVTAK.L 437 622.3530 1242.6914 1242.6819 7.62 0 75 1.7e-006 1 U K.AAQLATEIADIK.S 502 657.8478 1313.6810 1313.6575 17.8 0 58 8.5e-005 1 U R.EAQNDLEQVLR.Q 626 548.2989 1641.8748 1641.8434 19.1 1 36 0.012 1 U K.ASKEQALQDLQQQR.Q

7. UGGG1_HUMAN Mass: 177078 Score: 176 Matches: 10(5) Sequences: 10(5) emPAI: 0.13 UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens GN=UGGT1 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 249 468.2798 934.5450 934.5335 12.3 0 14 2.7 1 U K.AITTSLTTK.W 280 483.7785 965.5424 965.5289 14.0 0 13 2.7 1 U R.IMMLSVLK.N + 2 Oxidation (M)

Downloaded from iovs.arvojournals.org on 10/02/2021 4 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

300 499.2907 996.5668 996.5505 16.3 0 11 4.2 1 U K.ANPGAWILR.L 306 502.2757 1002.5369 1002.5168 20.1 0 19 0.83 1 U R.ISMINNPAK.E + Oxidation (M) 310 504.7765 1007.5384 1007.5288 9.54 0 51 0.00059 1 U R.FTILDSQGK.T 439 623.7911 1245.5677 1245.5666 0.86 0 30 0.066 1 U R.IEYQFFEDR.H 447 628.3478 1254.6811 1254.6608 16.2 0 47 0.0012 1 U K.ILETTTFFQR.A 559 700.3526 1398.6906 1398.6813 6.66 0 65 1.8e-005 1 U K.TAAVANSMNYLTK.K + Oxidation (M) 634 831.9162 1661.8179 1661.7937 14.5 0 35 0.015 1 U R.IVPEWQDYDQEIK.Q 642 852.4546 1702.8947 1702.8526 24.7 0 89 6e-008 1 U K.LNIQPSEADYAVDIR.S

8. A2MG_HUMAN Mass: 163188 Score: 165 Matches: 10(6) Sequences: 9(6) emPAI: 0.14 Alpha-2-macroglobulin OS=Homo sapiens GN=A2M PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 183 443.7284 885.4422 885.4345 8.76 0 17 1.5 1 K.YGAATFTR.T 363 552.3136 1102.6126 1102.5982 13.0 0 3 34 2 R.SSGSLLNNAIK.G 370 558.8181 1115.6216 1115.5975 21.6 0 58 0.00011 1 U R.QTVSWAVTPK.S 379 574.8205 1147.6265 1147.6138 11.1 0 32 0.037 1 U R.QGIPFFGQVR.L 380 574.8205 1147.6265 1147.6138 11.1 0 (19) 0.84 1 U R.QGIPFFGQVR.L 409 605.8348 1209.6551 1209.6353 16.3 0 42 0.0035 1 U K.LPPNVVEESAR.A 446 628.3336 1254.6527 1254.6357 13.5 0 53 0.00034 1 U K.AIGYLNTGYQR.Q 458 638.3043 1274.5941 1274.5602 26.6 0 80 6.3e-007 1 U R.VGFYESDVMGR.G + Oxidation (M) 602 515.9540 1544.8403 1544.7947 29.5 0 33 0.03 1 U R.LVHVEEPHTETVR.K 653 857.4171 1712.8197 1712.8291 -5.49 0 8 7.8 1 U K.SSSNEEVMFLTVQVK.G + Oxidation (M)

9. ALBU_HUMAN Mass: 69321 Score: 134 Matches: 3(3) Sequences: 3(3) emPAI: 0.17 Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 240 464.2514 926.4882 926.4861 2.18 0 30 0.059 1 U K.YLYEIAR.R 597 756.4295 1510.8445 1510.8355 5.92 0 85 1.7e-007 1 U K.VPQVSTPTLVEVSR.N 621 547.3290 1638.9653 1638.9305 21.2 1 71 4.2e-006 1 U K.KVPQVSTPTLVEVSR.N

10. K2C6B_HUMAN Mass: 60030 Score: 114 Matches: 5(5) Sequences: 5(5) emPAI: 0.35 Keratin, type II cytoskeletal 6B OS=Homo sapiens GN=KRT6B PE=1 SV=5 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 366 554.2852 1106.5558 1106.5356 18.3 0 33 0.03 1 K.AQYEEIAQR.S 396 590.3155 1178.6164 1178.5931 19.7 0 69 8.5e-006 1 K.YEELQITAGR.H 406 602.3331 1202.6516 1202.6295 18.3 0 33 0.033 1 K.WTLLQEQGTK.T 516 665.3767 1328.7388 1328.7187 15.1 0 58 0.00011 1 R.NLDLDSIIAEVK.A 567 704.3641 1406.7137 1406.7041 6.77 0 39 0.0074 1 K.ADTLTDEINFLR.A

Downloaded from iovs.arvojournals.org on 10/02/2021 5 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

11. SPTA2_HUMAN Mass: 284364 Score: 87 Matches: 6(2) Sequences: 6(2) emPAI: 0.03 Spectrin alpha chain, brain OS=Homo sapiens GN=SPTAN1 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 106 425.2000 848.3854 848.4538 -80.56 1 8 10 5 U R.AMRSQLK.K + Oxidation (M) 293 495.7772 989.5398 989.5294 10.5 0 43 0.004 1 U K.LFGAAEVQR.F 352 540.3046 1078.5947 1078.5811 12.6 0 15 2.1 1 U R.QGFVPAAYVK.K 450 631.8101 1261.6057 1261.5799 20.5 0 5 19 7 U R.EANQQQQFNR.N 490 651.8390 1301.6635 1301.6463 13.3 0 75 1.8e-006 1 U K.VLETAEDIQER.R 667 579.9626 1736.8660 1736.8217 25.5 1 10 4.4 1 U K.TATDEAYKDPSNLQGK.V

12. K2C5_HUMAN Mass: 62340 Score: 80 Matches: 4(3) Sequences: 4(3) emPAI: 0.19 Keratin, type II cytoskeletal 5 OS=Homo sapiens GN=KRT5 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 318 508.7782 1015.5418 1015.5298 11.8 0 13 3.8 1 R.QLDSIVGER.G 360 547.2715 1092.5284 1092.5199 7.76 0 46 0.0014 1 U K.AQYEEIANR.S 406 602.3331 1202.6516 1202.6295 18.3 0 33 0.033 1 K.WTLLQEQGTK.T 516 665.3767 1328.7388 1328.7187 15.1 0 58 0.00011 1 R.NLDLDSIIAEVK.A

13. K1H2_HUMAN Mass: 50310 Score: 41 Matches: 3(1) Sequences: 3(1) emPAI: 0.07 Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 19 404.2074 806.4002 806.3923 9.91 0 41 0.0055 1 K.LAADDFR.A 486 649.2909 1296.5673 1296.6422 -57.77 1 9 6.8 1 U K.KNHEEEVGSLR.C 533 450.1000 1347.2782 1347.5734 -219.07 0 1 31 8 U R.TVGMPCSPCPQGR.Y + Oxidation (M)

14. K1C24_HUMAN Mass: 55053 Score: 41 Matches: 2(1) Sequences: 2(1) emPAI: 0.07 Keratin, type I cytoskeletal 24 OS=Homo sapiens GN=KRT24 PE=1 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 19 404.2074 806.4002 806.3923 9.91 0 41 0.0055 1 R.LAADDFR.L 392 586.3228 1170.6310 1170.5921 33.2 0 7 12 1 U K.YYSIIEDLR.N

15. AP1B1_HUMAN Mass: 104570 Score: 39 Matches: 2(1) Sequences: 2(1) emPAI: 0.04 AP-1 complex subunit beta-1 OS=Homo sapiens GN=AP1B1 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Downloaded from iovs.arvojournals.org on 10/02/2021 6 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 334 525.2465 1048.4785 1048.5951 -111.14 1 4 30 2 U K.KVIASMTVGK.D + Oxidation (M) 608 796.8990 1591.7835 1591.8093 -16.20 1 39 0.0064 1 U K.GEIFELKAELNSDK.K

16. K22O_HUMAN Mass: 65800 Score: 33 Matches: 2(1) Sequences: 2(1) emPAI: 0.06 Keratin, type II cytoskeletal 2 oral OS=Homo sapiens GN=KRT76 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 366 554.2852 1106.5558 1106.5356 18.3 0 33 0.03 1 R.AQYEEIAQR.S 690 451.1000 1800.3709 1800.7818 -228.18 1 15 2.3 1 U R.MSCVARSGGAGGGACGFR.S + Oxidation (M); 2 Propionamide (C)

17. PUR2_HUMAN Mass: 107699 Score: 29 Matches: 2(0) Sequences: 1(0) emPAI: 0.03 Trifunctional purine biosynthetic protein adenosine-3 OS=Homo sapiens GN=GART PE=1 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 536 451.2671 1350.7796 1350.7184 45.3 0 29 0.075 1 U K.VDLGGFAGLFDLK.A 539 451.3000 1350.8782 1350.7184 118 0 (7) 11 9 U K.VDLGGFAGLFDLK.A

18. PLCB1_HUMAN Mass: 138480 Score: 24 Matches: 2(0) Sequences: 2(0) emPAI: 0.03 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Homo sapiens GN=PLCB1 PE=1 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 343 534.7620 1067.5094 1067.5070 2.30 0 24 0.26 1 U R.YVNLMEQR.A + Oxidation (M) 684 596.3161 1785.9263 1785.9360 -5.39 1 2 35 4 U R.AKQLAALTLEDEEEVK.K

19. RPA1_HUMAN Mass: 194687 Score: 16 Matches: 4(0) Sequences: 4(0) emPAI: 0.02 DNA-directed RNA polymerase I subunit RPA1 OS=Homo sapiens GN=POLR1A PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 53 414.2508 826.4870 826.4232 77.3 1 16 1.2 1 U K.AHMNAKR.C 408 604.8200 1207.6254 1207.5867 32.1 0 6 17 2 U R.TALSAVDMTQR.E + Oxidation (M) 715 467.3000 1865.1709 1864.9287 130 1 7 10 5 U K.CLRPEDILRFMETR.F + Oxidation (M); Propionamide (C) 844 669.3677 2673.4415 2673.4126 10.8 1 1 37 9 U R.LYSNDIHAIANTYGIEAALRVIEK.E

Peptide matches not assigned to protein hits: (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 283 485.3029 968.5912 968.5767 14.9 0 30 0.062 1 VAVVNQIAR

Downloaded from iovs.arvojournals.org on 10/02/2021 7 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

854 721.3580 2881.4028 2881.4709 -23.60 0 23 0.2 1 QQQLEEEAAKPPEPEKPVSPPPIESK 336 527.2975 1052.5804 1052.5655 14.2 0 21 0.43 1 VIFLENYR 91 421.7632 841.5118 841.5021 11.5 1 21 0.48 1 ELKSIPR 178 442.7624 883.5102 883.5565 -52.35 0 21 0.48 1 VLPCIVIK 836 646.2931 2581.1435 2581.1748 -12.13 1 20 0.38 1 GASVSINVNTSLEDMRSNNVEDCK 603 783.8985 1565.7824 1565.7937 -7.20 1 20 0.53 1 FQESQEEIKSLTK 433 414.2797 1239.8172 1239.6935 99.8 0 20 0.61 1 ILIGGNLPGSSGR 732 490.2924 1957.1407 1956.9866 78.7 0 20 0.45 1 VMDIPYLNLEGPDLQPK + Oxidation (M) 346 536.2967 1070.5788 1070.6237 -41.91 0 20 0.72 1 LLALAGGPGFR 50 413.2000 824.3854 824.3817 4.55 0 19 0.63 1 APDGFYR 457 425.2000 1272.5782 1272.7150 -107.49 1 19 0.81 1 ELVRSISTNVR 703 606.2144 1815.6214 1815.9528 -182.46 1 19 0.67 1 MPHAFKPGDLVFAKMK 546 453.2911 1356.8516 1356.6885 120 0 18 0.86 1 LAEELGQAEELR 49 412.7503 823.4860 823.4552 37.4 0 18 0.83 1 HIQGLEK 680 445.1305 1776.4927 1776.8750 -215.14 0 18 0.88 1 IMASSPDMDLATVSALR 44 411.1469 820.2793 820.3749 -116.47 0 18 0.98 1 ACSQGEVK 400 596.3161 1190.6176 1190.5965 17.7 0 18 0.94 1 LTLSMRPDDK + Oxidation (M) 375 571.2000 1140.3854 1140.6139 -200.27 1 18 0.9 1 VPDNKNVLDK 825 613.3162 2449.2356 2449.2641 -11.66 1 18 0.59 1 SDFPLLAPPTNYGLYEQLKQR 330 523.2992 1044.5838 1044.5788 4.74 1 18 1.1 1 SISRPRSSR 167 439.2000 876.3854 876.4011 -17.83 0 18 1.2 1 AEAEQMAK 635 417.1000 1664.3709 1664.8482 -286.68 1 18 0.92 1 THLAPGVERQTAEEK 277 482.3085 962.6024 962.5297 75.5 1 17 1.3 1 AAGFLRSNK 290 492.2383 982.4621 982.4794 -17.58 0 17 1.1 1 FTMDVIGGK + Oxidation (M) 588 740.4067 1478.7989 1478.7803 12.6 0 17 1.1 1 VVTYGMANLLTGPK + Oxidation (M) 691 451.2671 1801.0394 1800.9218 65.3 1 17 1 1 AGVDTSPDTQKILVDSR 387 582.3253 1162.6361 1162.5699 56.9 1 17 1.3 1 LVGGTNRCMGR 584 492.5868 1474.7386 1474.7198 12.8 1 17 1.1 1 MRQIASNSPGSSPK + Oxidation (M) 410 606.2000 1210.3854 1210.5870 -166.47 0 17 1.1 1 AFAEGFLAEEK 776 737.7217 2210.1431 2210.0644 35.6 1 17 0.87 1 TVNLTDFSNKWDSTNPFPK 675 587.2000 1758.5782 1758.8359 -146.54 0 17 1.1 1 VPSMGPPSKPPDEEHR 648 571.2000 1710.5782 1710.8069 -133.71 1 16 1.1 1 LVGVESACDVDCRFK + Propionamide (C) 508 441.1000 1320.2782 1320.5988 -242.82 0 16 0.8 1 TPCLPGTCSCLR + Propionamide (C) 728 484.2072 1932.7999 1932.9640 -84.91 1 16 1.1 1 KEAVSVLDSADIEVTDSR 423 411.1000 1230.2782 1230.4753 -160.18 0 16 1.1 1 MMMGCGESELK + Oxidation (M) 820 606.2000 2420.7709 2420.1327 264 1 16 1.7 1 YEFSPQTLCCYGKQLCTIPR + Propionamide (C) 215 453.1481 904.2817 904.4767 -215.52 0 16 1.7 1 AFVGEVGAR 158 436.2275 870.4404 870.4923 -59.65 1 16 1.6 1 KAVPGNSAK 403 401.1000 1200.2782 1200.5775 -249.32 0 16 0.97 1 DLEFHAVQDK 30 408.2039 814.3931 814.4800 -106.61 0 16 1.7 1 ILAEELK 823 611.2000 2440.7709 2440.2203 226 1 16 1.8 1 VVQLSDACDMLGDHIVLDSVRR 456 425.2000 1272.5782 1272.6860 -84.74 0 16 1.5 1 MVVQVVIGEQR + Oxidation (M) 695 451.3000 1801.1709 1800.8941 154 1 16 1.3 1 LQINSPPFDQPMSRR + Oxidation (M) 166 439.2000 876.3854 876.5103 -142.39 1 16 1.8 1 AMTGLIKK + Oxidation (M) 376 571.8712 1141.7278 1141.6343 81.9 0 16 1.7 1 NIPQSTEILK 638 425.2000 1696.7709 1696.8672 -56.75 1 16 1.4 1 VEDLVKQYFQTAEK 561 467.3000 1398.8782 1398.8235 39.1 1 16 1.5 1 VIYINSPLPQKK

Downloaded from iovs.arvojournals.org on 10/02/2021 8 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

101 424.2615 846.5085 846.4671 48.9 1 16 2 1 LSRQSTR 67 417.1000 832.1854 832.4113 -271.27 0 16 1.2 1 ELAQCAK + Propionamide (C) 263 475.2270 948.4394 948.4553 -16.69 0 16 2 1 DEQVPFSK 512 442.7903 1325.3490 1325.5315 -137.70 0 15 1.4 1 SCCPGWSAMAR + Oxidation (M); 2 Propionamide (C) 688 449.3000 1793.1709 1792.8699 168 0 15 1.4 1 IMASSPDMDLATVSALR + Oxidation (M) 411 606.2331 1210.4516 1210.5870 -111.85 0 15 1.6 1 AFAEGFLAEEK 589 740.4093 1478.8040 1478.7803 16.0 0 15 1.6 1 VVTYGMANLLTGPK + Oxidation (M) 135 432.2374 862.4602 862.4041 65.1 1 15 2.2 1 EMPGCKK + Propionamide (C) 666 435.1000 1736.3709 1736.8776 -291.71 1 15 1.7 1 LLPCPRCYGILTCSK + Propionamide (C) 270 478.2676 954.5207 954.5862 -68.61 0 15 1.7 1 SKPQIAALK 554 462.1000 1383.2782 1383.6309 -254.90 0 15 0.95 1 NLTAGMAMITCR + 2 Oxidation (M); Propionamide (C) 833 846.2401 2535.6985 2535.3234 148 1 15 2.1 1 VHHPDYNNELTQFLPRTITLK 611 539.3000 1614.8782 1614.8763 1.17 1 15 1.6 1 MELLEDALVLRSAR 232 460.2951 918.5757 918.4606 125 1 15 2.3 1 AHRCHPK + Propionamide (C) 673 439.2000 1752.7709 1752.8426 -40.93 0 15 1.4 1 VTCVEMEPLAEYVVR + Oxidation (M) 206 451.1000 900.1854 900.4487 -292.37 0 15 0.71 1 LMSLEHR + Oxidation (M) 529 449.0000 1343.9782 1343.7310 184 1 15 1.7 1 VRKPNDLEAFR 476 430.9000 1289.6782 1289.5566 94.2 0 15 1.8 1 HNITMCPLCDK + Oxidation (M) 495 653.3640 1304.7135 1304.7466 -25.34 1 15 1.8 1 IDFHLHVLRR 177 442.2489 882.4833 882.5651 -92.59 1 15 1.8 1 KSIPALVR 414 611.2000 1220.3854 1220.6513 -217.81 1 15 1.9 1 AIGPHLAKDGDK 506 439.2000 1314.5782 1314.6793 -76.90 1 15 2 1 RWGQVSQAVER 713 617.9898 1850.9474 1850.9924 -24.32 1 15 1.6 1 GTGLFCSTTQGKILVQK + Propionamide (C) 54 414.2797 826.5448 826.5388 7.22 1 15 1.8 1 RIANLIK 472 430.1000 1287.2782 1287.6221 -267.12 0 15 1.1 1 GAGGHPQFAPHGR 704 611.2000 1830.5782 1830.8855 -167.88 1 15 1.6 1 ECTALDAHKELEMVVK + Oxidation (M) 278 482.7636 963.5126 963.4695 44.8 0 15 2.3 1 LEMEISAR + Oxidation (M) 234 461.7495 921.4845 921.4113 79.5 0 15 2.1 1 EAMEADLK + Oxidation (M) 62 416.2287 830.4429 830.3341 131 0 14 2.8 1 HMENER + Oxidation (M) 623 411.1000 1640.3709 1639.8967 289 0 14 1.7 1 NLPLDVAIECLTLR + Propionamide (C) 208 451.2671 900.5197 900.4777 46.7 1 14 2.8 1 ALEEGARR 454 425.2000 1272.5782 1272.7289 -118.45 1 14 2.3 1 DKVDVASVIVTK 765 539.2035 2152.7848 2153.0869 -140.30 0 14 1.8 1 LCTCTMLMLIGLGVLAWQR + 2 Oxidation (M) 415 611.3591 1220.7036 1220.6699 27.6 1 14 2.3 1 VNQGLIRMYK 824 611.2678 2441.0423 2441.3947 -144.35 1 14 1.7 1 VAILAQLYHEKPLVFLERFR 440 416.2000 1245.5782 1245.6362 -46.59 1 14 2.4 1 LMTLFMRYR + Oxidation (M) 796 578.1573 2308.6002 2308.1733 185 0 14 2.9 1 LSESPASLPSCPPVETALINQR 312 507.1982 1012.3818 1012.5957 -211.23 0 14 2.3 1 DLALLFPPK 828 625.2758 2497.0743 2497.4488 -149.96 1 14 1.4 1 MAVMAPRTLLLLLLGALALTQTR + 2 Oxidation (M) 182 443.2383 884.4620 884.4716 -10.78 0 14 2.3 1 APEAGATLR 622 411.1000 1640.3709 1639.8967 289 0 14 1.9 1 NLPLDVAIECLTLR + Propionamide (C) 416 612.3115 1222.6084 1222.5587 40.7 0 14 2.5 1 AQVFSCPACR + 2 Propionamide (C) 542 453.2000 1356.5782 1356.7013 -90.73 1 14 2.4 1 AGAVPKMFFFSR 133 432.1000 862.1854 862.3603 -202.78 0 14 1.7 1 AGMGDSPGR + Oxidation (M) 543 453.2106 1356.6099 1356.7302 -88.70 0 14 2.5 1 NYLVHAVSWLR 500 655.3339 1308.6533 1308.7150 -47.15 1 14 2.4 1 AKTHVLDIEQR 122 428.7670 855.5194 855.4273 108 0 14 2.7 1 SPSCPLPR

Downloaded from iovs.arvojournals.org on 10/02/2021 9 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

681 593.2267 1776.6583 1776.8642 -115.89 0 14 2.5 1 TSGPLGNLAEELNGYSR 562 467.3000 1398.8782 1398.8235 39.1 1 14 2.5 1 VIYINSPLPQKK 259 472.2845 942.5544 942.5247 31.5 1 14 2.7 1 AQSSAPKVR 556 462.1000 1383.2782 1383.6857 -294.50 0 13 1.5 1 MSFSSQVATLWK 658 431.2093 1720.8082 1720.7726 20.7 1 13 2.7 1 KENYGSITSMGYESR 393 587.2000 1172.3854 1172.6230 -202.58 0 13 3.2 1 TILGGFQYFK 268 477.2606 952.5066 952.4475 62.1 0 13 2.8 1 EHQQQQR 716 467.3000 1865.1709 1864.8890 151 0 13 2.3 1 LHSHVENMLDLAWER + Oxidation (M) 489 434.8809 1301.6210 1301.6324 -8.79 1 13 2.8 1 RSVQSGETGPER 709 462.1460 1844.5551 1844.7492 -105.21 0 13 2.4 1 QEMTSTVYCHGGGSCK + Oxidation (M); 2 Propionamide (C) 662 433.1000 1728.3709 1727.9570 240 1 13 2.8 1 VLGQYLRDTAALSPPK 569 471.1000 1410.2782 1410.5821 -215.44 0 13 1.3 1 DDGSTLMEIDGDK + Oxidation (M) 518 667.3377 1332.6608 1332.6397 15.8 1 13 3 1 YGSAVFRGCFR + Propionamide (C) 645 427.2892 1705.1278 1704.9306 116 0 13 2.6 1 FMPAGLIAGASLLMVAK + Oxidation (M) 696 451.3000 1801.1709 1800.8395 184 0 13 2.7 1 QLLAMLMLFCDCSR + Oxidation (M); 2 Propionamide (C) 402 600.8341 1199.6536 1199.6761 -18.74 1 13 3.1 1 EINDLLVKEK 100 424.2615 846.5085 846.4671 48.9 1 13 3.9 1 LSRQSTR 655 430.1000 1716.3709 1716.8393 -272.82 0 13 2.9 1 MSTIGSFEGFQAVSLK + Oxidation (M) 727 482.1464 1924.5566 1924.9901 -225.19 1 13 3 1 VGLALPGGRLGCGEAGSANR + Propionamide (C) 672 439.2000 1752.7709 1752.8069 -20.56 1 13 2.4 1 CECTVQTRAVACTR + 3 Propionamide (C) 598 507.2000 1518.5782 1518.5320 30.4 0 13 2.7 1 SSAGSDGCGSMCCGR + 2 Propionamide (C) 605 525.2000 1572.5782 1572.7685 -121.01 1 13 2.8 1 SPGQNWVKTADGWK 549 459.3000 1374.8782 1374.7296 108 0 13 3.3 1 DVLHGVFNYLAK 577 720.4156 1438.8167 1438.7061 76.9 0 13 3.1 1 APLIPMEHCTTR + Propionamide (C) 173 441.2000 880.3854 880.4333 -54.32 1 13 3.3 1 VCMVCKK + Propionamide (C) 195 447.3000 892.5854 892.4072 200 0 13 3.7 1 TAEAAMQR + Oxidation (M) 540 451.3000 1350.8782 1350.6826 145 1 13 3.1 1 ACGALRNLSYGR + Propionamide (C) 715 467.3000 1865.1709 1864.7931 203 1 13 2.7 1 SPLKMSSNGCENDPGDSK 103 425.2000 848.3854 848.3876 -2.49 1 13 3.9 1 GDESKDAK 471 429.7823 1286.3251 1286.6078 -219.72 0 13 2.7 1 HCTPAWTTTAK + Propionamide (C) 368 556.3005 1110.5864 1110.5782 7.47 1 13 3.1 1 HSVDELQKR 790 571.2000 2280.7709 2281.0937 -141.49 0 12 3.7 1 VEQVPIVDIMFNDFGEASQK + Oxidation (M) 443 417.1000 1248.2782 1248.5743 -237.21 0 12 2.5 1 NFMEHGLMVR + Oxidation (M) 517 667.3352 1332.6559 1332.6067 36.9 1 12 3.5 1 SFQNIMRCYR + Oxidation (M) 37 409.2000 816.3854 816.4453 -73.36 1 12 4.5 1 AKGLGESR 420 409.2000 1224.5782 1224.7190 -115.02 0 12 3.4 1 VLIIGGGDGGVLR 708 462.1000 1844.3709 1844.8298 -248.74 0 12 4.1 1 ANNPWPASTSCCVPTAR + Propionamide (C) 778 557.1912 2224.7355 2225.0683 -149.54 1 12 3.8 1 MMDFETFLPMLQHISKNK + Oxidation (M) 153 435.2243 868.4340 868.3902 50.5 0 12 3.1 1 AYFHMGK + Oxidation (M) 251 469.2000 936.3854 936.5253 -149.38 1 12 3.7 1 AGPGVGAPRR 674 439.2000 1752.7709 1752.8941 -70.28 1 12 2.9 1 VEQMPQASPGLAPRTR + Oxidation (M) 798 583.2549 2328.9904 2329.1195 -55.42 1 12 2.4 1 ETCPGWVNKNKPALEQDVCK + Propionamide (C) 362 552.3024 1102.5903 1102.5506 36.1 1 12 4.4 1 EQEEKAEIK 24 405.1894 808.3643 808.3571 8.89 0 12 4.6 1 MTCDIR + Propionamide (C) 355 543.2000 1084.3854 1084.5488 -150.60 0 12 4.2 1 FVIHHSMSK 176 442.2171 882.4197 882.4633 -49.43 1 12 3.5 1 KLMAYGGK + Oxidation (M) 448 629.8190 1257.6234 1257.6214 1.60 0 12 4.1 1 NLNGHSIGQYR

Downloaded from iovs.arvojournals.org on 10/02/2021 10 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

616 409.1000 1632.3709 1632.7453 -229.34 0 12 3 1 NADAPSPQAFDEMLK 803 587.2000 2344.7709 2345.0919 -136.90 0 12 4.6 1 TGMLTITLDESDCFAAWVSAK + Oxidation (M); Propionamide (C) 837 651.2378 2600.9219 2601.2605 -130.17 1 12 4.3 1 RVSTDLPEGQDVYTAACNSVIHR + Propionamide (C) 398 592.8719 1183.7293 1183.5465 154 0 12 4.2 1 TMEDTLMTVK + Oxidation (M) 417 409.1000 1224.2782 1224.6098 -270.84 0 12 2.6 1 DISEEVQLHR 185 444.2367 886.4589 886.5674 -122.40 1 12 5.2 1 LICVKALK 189 445.2642 888.5138 888.5103 4.03 0 12 5.2 1 IVCLNSLK 613 543.2000 1626.5782 1626.8577 -171.83 1 12 3.5 1 TTDLTTERLVHVDK 736 504.2000 2012.7709 2012.9699 -98.88 1 12 3.1 1 EREFLFNAIETMPCVK + Oxidation (M); Propionamide (C) 643 427.1000 1704.3709 1703.9102 270 0 12 4 1 NLPMISFLILPMQR + 2 Oxidation (M) 46 411.2000 820.3854 820.3385 57.2 0 12 4.7 1 CPSGDSAGK 282 484.7570 967.4994 967.6178 -122.37 1 12 3.7 1 SILPKAALR 814 593.2935 2369.1450 2369.0416 43.7 1 12 2.5 1 THSGEKPFECNECEKAFSQK + Propionamide (C) 179 442.7883 883.5620 883.5280 38.5 0 12 3.9 1 VPLPFGVR 505 439.2000 1314.5782 1314.6391 -46.31 0 12 4.2 1 FLQMNGYAVTR + Oxidation (M) 105 425.2000 848.3854 848.4756 -106.25 0 12 4.8 1 AGIFQSVK 374 567.3351 1132.6557 1132.4633 170 0 12 4.5 1 QTESDPDADR 636 417.1000 1664.3709 1664.7724 -241.19 1 12 3.7 1 GMKIYYMQVQMNK + 2 Oxidation (M) 629 414.2797 1653.0896 1652.8054 172 1 12 3.6 1 MHAAASLFEVYCKI + Propionamide (C) 789 570.2818 2277.0982 2276.7926 134 0 11 2.6 1 CDADADCMDGSDEEACGTGVR + Oxidation (M); 2 Propionamide (C) 843 667.2008 2664.7742 2665.1658 -146.94 1 11 4.7 1 SVIKGAHYICHSGGHCPMDTYMR + 2 Oxidation (M); Propionamide (C) 702 606.2000 1815.5782 1815.7776 -109.83 0 11 3.5 1 NFVDLACECSAVICCR + Propionamide (C) 712 463.1309 1848.4945 1848.8572 -196.16 1 11 5 1 MLMNARYFLEMSDTK 412 405.2351 1212.6836 1212.5411 118 1 11 4.2 1 DEEENFFKR 201 449.3000 896.5854 896.4538 147 1 11 3.8 1 LGSSFCKR 692 451.3000 1801.1709 1800.6897 267 0 11 3.8 1 EYINECDSDYHEER 219 453.2126 904.4106 904.4866 -83.95 0 11 5.4 1 SLATTADVK 637 421.1000 1680.3709 1679.9942 224 1 11 3.7 1 LLALVPMGIPRTLMR 483 648.8354 1295.6562 1295.6833 -20.92 1 11 4.3 1 AELHQLSGGEKK 169 440.1446 878.2746 878.4280 -174.61 0 11 5.1 1 AVSMASAAR + Oxidation (M) 361 547.3069 1092.5992 1092.5564 39.2 1 11 4.6 1 DGGDVKALYR 453 634.3404 1266.6663 1266.6820 -12.33 1 11 4.1 1 GEGPEVDVKLPK 261 473.2595 944.5044 944.5226 -19.19 1 11 6 1 RGVLGMANK 519 445.3000 1332.8782 1332.5591 239 0 11 4.5 1 SHGFMDMLHDK + Oxidation (M) 97 424.1641 846.3137 846.4963 -215.78 0 11 5.8 1 ILVPSYR 275 482.2191 962.4237 962.5041 -83.52 0 11 5.3 1 AATLMMLGR 750 525.2000 2096.7709 2097.0201 -118.83 1 11 3.4 1 DPLMQWLGKHVDSEGEIK + Oxidation (M) 753 530.1755 2116.6727 2116.0623 288 1 11 4.7 1 ILQACTEKVDEHQTFLNK 141 434.2597 866.5048 866.4974 8.62 0 11 4.4 1 IHSLLER 418 409.1000 1224.2782 1224.6238 -282.20 1 11 3.1 1 ETITKETEFK 661 433.1000 1728.3709 1728.8471 -275.47 0 11 4.5 1 FNGDVLSPSYIQSFR 317 508.2640 1014.5135 1014.5458 -31.81 1 11 5.7 1 ALGEDIGGKR 571 472.9179 1415.7319 1415.7078 17.0 0 11 4.4 1 MVSIHSLSELER + Oxidation (M) 744 519.1458 2072.5540 2072.9738 -202.50 0 11 6.1 1 LLNCQEFAVDLEHHSYR 419 409.1000 1224.2782 1224.5162 -194.35 1 11 3.2 1 RCNDMMNLGR + Oxidation (M) 84 421.2116 840.4086 840.4858 -91.78 0 11 4.2 1 ALPGAWVK 347 536.3317 1070.6489 1070.5972 48.3 0 11 5.4 1 LQGTDIPTVK

Downloaded from iovs.arvojournals.org on 10/02/2021 11 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

771 542.2283 2164.8841 2165.0211 -63.30 1 11 3.3 1 DLPQQLKNPASMDADGFYR 323 516.3173 1030.6200 1030.4688 147 0 11 5.5 1 CACASHVAK + 2 Propionamide (C) 405 401.2400 1200.6982 1200.5847 94.6 1 11 4.9 1 VQRGDENDIR 714 467.3000 1865.1709 1864.8018 198 0 11 4 1 AHQYQITGCCLSPDCR + Propionamide (C) 780 557.2291 2224.8874 2225.0535 -74.65 1 11 3.6 1 VNSYTMKDTAAHGTIPNYSR 759 536.1762 2140.6756 2141.0622 -180.57 1 11 5.4 1 GCYIGQELTARTHHMGVIR 850 707.3023 2825.1800 2824.4793 248 1 11 4.5 1 LFVCISGVADQLQTNYASDLRSILK + Propionamide (C) 646 427.3000 1705.1709 1704.8691 177 1 11 4.3 1 RTPVQPNPIVYMMK + 2 Oxidation (M) 106 425.2000 848.3854 848.4240 -45.40 0 11 5.8 1 TVTSDQAK 481 432.8867 1295.6382 1295.7310 -71.59 1 11 4.8 1 LRDPGLQQTLR 615 409.1000 1632.3709 1632.7453 -229.34 0 11 3.9 1 NADAPSPQAFDEMLK 285 487.2117 972.4088 972.5426 -137.58 0 11 6 1 MLPLLSQR + Oxidation (M) 34 409.1000 816.1854 816.3735 -230.30 0 11 2.9 1 VHQMMR + Oxidation (M) 609 401.1000 1600.3709 1600.7481 -235.67 1 11 3.9 1 VDEEYKNPHTVDR 821 607.2351 2424.9112 2425.2828 -153.21 1 11 5 1 SGSVFIGGFPDLHGKIQMPVPVK + Oxidation (M) 487 651.3054 1300.5962 1300.6268 -23.53 1 11 5 1 QVDMDHVMKAK 247 467.3000 932.5854 932.4208 177 0 11 6.3 1 GMCVHATK + Oxidation (M); Propionamide (C) 431 413.2000 1236.5782 1236.7190 -113.88 1 11 4.9 1 GIIQKIVDSHK 307 502.2842 1002.5539 1002.5233 30.5 0 11 6 1 IDELIESGK 510 441.2000 1320.5782 1320.5988 -15.66 0 11 5 1 TPCLPGTCSCLR + Propionamide (C) 607 530.2800 1587.8182 1587.7868 19.8 0 11 4.7 1 MYPQLTFAPVHER 210 451.3000 900.5854 900.5215 71.0 0 11 6.6 1 LQMIQLR 700 453.2126 1808.8213 1808.8291 -4.33 1 11 4.3 1 YKYSLLTDGSWDMSK + Oxidation (M) 222 454.2625 906.5104 906.5399 -32.51 0 11 6.4 1 AALIVHQR 539 451.3000 1350.8782 1350.7064 127 1 11 5.1 1 MITSVLEKEASK + Oxidation (M) 617 409.1000 1632.3709 1632.6635 -179.25 0 10 4.2 1 YCYICHSMAYHR + Oxidation (M); Propionamide (C) 835 643.2975 2569.1610 2569.1222 15.1 0 10 3.2 1 MWDVSTGMCLMTLVGHDNWVR + 3 Oxidation (M); Propionamide (C) 140 434.2459 866.4772 866.4320 52.2 0 10 5.1 1 SLFQCAK + Propionamide (C) 187 444.2706 886.5267 886.4621 72.9 0 10 7.1 1 AATSRPER 211 451.3000 900.5854 900.4301 173 0 10 6.9 1 SHGALSDSK 689 450.1000 1796.3709 1795.8774 275 1 10 6.1 1 LENKIFSVTNNTECGK 722 471.1000 1880.3709 1879.9826 207 0 10 6.3 1 ARPGQVSSYMATVSGLLK + Oxidation (M) 144 435.1000 868.1854 868.3902 -235.73 0 10 2.5 1 EPCFFR + Propionamide (C) 163 438.2116 874.4086 874.5310 -139.98 1 10 7.2 1 MKIINLK + Oxidation (M) 25 405.1990 808.3834 808.4290 -56.52 1 10 6.7 1 TSTTSKGK 316 507.8184 1013.6222 1013.6485 -25.92 0 10 6.3 1 LLGLTGLLSK 792 767.4050 2299.1930 2298.9845 90.7 1 10 3.8 1 EYQTMRESQLQQEDPMDR + Oxidation (M) 252 469.3000 936.5854 936.4916 100 0 10 5.6 1 YTVLAENK 488 651.3585 1300.7024 1300.6299 55.7 0 10 5.5 1 ISVYYNEATGGK 325 518.2882 1034.5619 1034.5583 3.51 1 10 5.8 1 MVSSKGLWK 853 717.3667 2865.4377 2865.3936 15.4 1 10 3.4 1 SPPMLPVNGKMHSAVDCNGVVSLVDGR + Oxidation (M); Propionamide (C) 372 564.2927 1126.5708 1126.5731 -2.04 0 10 5.5 1 LLASATGHSDR 444 417.2194 1248.6365 1248.7078 -57.08 0 10 5.7 1 LQLPGAPSPLEK 527 447.5689 1339.6848 1339.5319 114 0 10 5.4 1 CADCQMQLADR + Oxidation (M); Propionamide (C) 604 784.3859 1566.7573 1566.7752 -11.44 0 10 5.2 1 AFDSGIIPMEFVNK 710 462.1501 1844.5713 1844.9124 -184.89 1 10 4.6 1 TVCSMGTGTSRLYSALAK 184 443.7782 885.5419 885.3940 167 0 10 6.7 1 SHSESPSR

Downloaded from iovs.arvojournals.org on 10/02/2021 12 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

773 547.2000 2184.7709 2185.1102 -155.29 0 10 5 1 CHLSSDSFIQIALQLAHFR 102 425.1954 848.3763 848.4392 -74.13 0 10 6.7 1 DPTLTFR 583 490.2924 1467.8555 1467.7140 96.4 1 10 4.9 1 NSGAPRTTYLASCK 697 452.3000 1805.1709 1804.9069 146 1 10 4.9 1 ADEQAFLVALYKYMK + Oxidation (M) 426 411.2000 1230.5782 1230.5993 -17.15 1 10 6.3 1 YKHVEQNGEK 383 579.7963 1157.5781 1157.5764 1.46 1 10 6.6 1 WVGARMGEPR 216 453.1784 904.3422 904.5606 -241.54 1 10 7.2 1 AHLRAPLK 842 660.3081 2637.2031 2637.5114 -116.87 1 10 3.9 1 MSLPLNPKPFLNGLTGKPVMVKLK + Oxidation (M) 353 541.2484 1080.4823 1080.5386 -52.06 1 10 5.9 1 ACERALQYK 627 413.2000 1648.7709 1648.8130 -25.56 1 10 5.2 1 AEKAMVDGSWLDLAK + Oxidation (M) 663 577.2000 1728.5782 1728.8366 -149.47 0 10 5.1 1 VHQSIHTGEKPHECK 807 593.1599 2368.6104 2369.1070 -209.60 1 10 7.3 1 GGDAASHLFTASLGGKNQYSSCK + Propionamide (C) 532 449.3000 1344.8782 1344.6521 168 1 10 6 1 QPLTSKEEEER 350 538.2944 1074.5742 1074.5491 23.4 1 10 7 1 KPPMSEKSR + Oxidation (M) 463 427.3000 1278.8782 1278.5095 288 0 10 5.7 1 MSHGHSHGGGGCR 694 451.3000 1801.1709 1800.8941 154 1 10 5.3 1 LQINSPPFDQPMSRR + Oxidation (M) 104 425.2000 848.3854 848.4109 -29.99 1 10 7.1 1 ACRVCR + 2 Propionamide (C) 76 421.1999 840.3853 840.4566 -84.79 1 10 5.4 1 APERVGGR 61 416.2000 830.3854 830.4610 -90.97 1 10 8 1 AAGLSRGLS 288 491.2104 980.4062 980.5403 -136.75 1 10 6.2 1 RAGPATSPPK 171 441.1000 880.1854 880.4014 -245.27 0 10 3.9 1 AFWCQR + Propionamide (C) 576 718.3907 1434.7669 1434.7943 -19.09 1 10 5.8 1 QTGRVLEPPVPSR 594 752.8794 1503.7443 1503.7140 20.2 0 10 5.6 1 HEVTAVFAADAMAR + Oxidation (M) 35 409.1000 816.1854 816.4242 -292.45 0 10 3.6 1 NWSAIAR 677 441.2000 1760.7709 1760.8628 -52.19 1 10 5.2 1 VTSQCLDQNGHKLYR 530 449.2176 1344.6309 1344.6608 -22.28 1 10 6.3 1 EGMHLKNVDFR 739 513.2950 2049.1508 2049.0756 36.7 1 10 4.7 1 LPAPPSSSSPTVVFQSHRR 784 746.3910 2236.1511 2236.0987 23.4 0 10 4.5 1 GLVYIQQTDDSLIHFCWK + Propionamide (C) 242 466.2307 930.4468 930.4957 -52.45 1 10 7.9 1 MEPSALRK 581 487.2117 1458.6132 1458.6314 -12.45 1 10 6.2 1 KMMMKPSCSWCK 570 471.1000 1410.2782 1410.6700 -277.79 0 10 3 1 MSYAEKPDEITK 507 439.2000 1314.5782 1314.6979 -91.05 1 9 6.9 1 MLGRLAQAYHR 754 530.2800 2117.0910 2116.9491 67.0 1 9 4.5 1 MLRCGAELGSPGGGGGGGGGGGAGGR + Oxidation (M) 785 562.1104 2244.4125 2244.1862 101 1 9 6.9 1 HPSLKPSTEVEDPAVKGAVQR 698 453.2000 1808.7709 1808.9268 -86.20 1 9 5.7 1 SEILLNQKTTQDTYR 786 563.2459 2248.9545 2249.0369 -36.64 0 9 4.6 1 MSGEDEQQEQTIAEDLVVTK 612 541.2484 1620.7235 1620.8657 -87.77 0 9 6 1 LLQMQPAQHGEIIK + Oxidation (M) 664 434.2341 1732.9072 1732.7878 68.9 0 9 6 1 GDTPGHATPGHGGATSSAR 245 467.3000 932.5854 932.4199 178 0 9 8.7 1 AEGQASENK 656 430.8931 1719.5433 1719.8086 -154.27 1 9 6.6 1 LHAWRAGCAGSVCYR + Propionamide (C) 254 470.2410 938.4675 938.4644 3.28 0 9 6.9 1 TLCGAFTR + Propionamide (C) 156 435.7810 869.5475 869.4971 58.0 0 9 6.8 1 VVDVSVPR 248 468.2654 934.5163 934.5124 4.23 0 9 9 1 YDLGQVIK 748 519.1479 2072.5626 2072.7784 -104.07 0 9 9.2 1 FHHCDMYGSACADCCLAR + Propionamide (C) 160 437.2674 872.5203 872.4716 55.8 0 9 9.8 1 AGDTVGVVR 256 471.1000 940.1854 940.3808 -207.70 0 9 2.8 1 TSEMNTDK + Oxidation (M) 125 429.2431 856.4717 856.4589 14.9 0 9 8.4 1 MILSTHR

Downloaded from iovs.arvojournals.org on 10/02/2021 13 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

258 472.2619 942.5092 942.5134 -4.51 0 9 7.5 1 AAAEELLAR 321 515.2838 1028.5530 1028.4307 119 0 9 7.9 1 ADCLMGEFK + Oxidation (M) 327 521.2712 1040.5278 1040.4637 61.6 0 9 7.1 1 YMWEGEVK 228 458.7498 915.4851 915.4774 8.43 1 9 8.8 1 ARAELNDK 364 553.7931 1105.5716 1105.5689 2.44 0 9 8.5 1 LVEDILSSCK 273 480.2766 958.5386 958.5196 19.9 1 9 9.1 1 AQAQSQKAK 578 721.4000 1440.7854 1440.6528 92.1 1 9 6.7 1 MNSQNSGFTQRR + Oxidation (M) 501 437.2674 1308.7804 1308.6245 119 1 9 7.2 1 CDSIQFAVDRR 841 659.3627 2633.4219 2633.3463 28.7 1 9 5.1 1 EFSQSLHLQTHQRVHTGEKPFK 699 453.2106 1808.8132 1808.8284 -8.42 0 9 6.4 1 AEENLLMVLGTDMSDR + Oxidation (M) 217 453.2000 904.3854 904.4688 -92.15 1 9 9.4 1 ELKGGCVIS 313 507.2000 1012.3854 1012.4872 -100.51 0 9 7.5 1 AHTAAMANAR 424 411.1000 1230.2782 1230.5162 -193.40 0 9 5.9 1 YHGDPMPCPK + Oxidation (M); Propionamide (C) 250 469.2000 936.3854 936.4777 -98.52 1 9 7.8 1 ADYSIGRR 724 471.1000 1880.3709 1880.0050 195 1 9 9 1 VLRSLCSLAAEGIQHQR 528 449.0000 1343.9782 1343.6907 214 0 9 7 1 FEVALLPEPCR + Propionamide (C) 537 451.3000 1350.8782 1350.6575 163 1 9 7.5 1 SPHTHSMARGVR + Oxidation (M) 319 513.2821 1024.5497 1024.5705 -20.36 1 9 7.4 1 IQAAFKGYK 404 401.1000 1200.2782 1200.6040 -271.38 1 9 5.3 1 GQGRWEFPPK 48 412.2392 822.4638 822.4712 -8.98 0 9 8 1 VHDLALR 233 461.2557 920.4968 920.3442 166 0 9 9.1 1 MFEMGMK + 3 Oxidation (M) 425 411.1000 1230.2782 1230.6469 -299.63 1 9 6 1 QPAGFREGITR 591 498.2596 1491.7570 1491.8548 -65.60 1 9 7.4 1 SKDFLSLLLESLK 365 554.2718 1106.5291 1106.5754 -41.80 1 9 8.4 1 LQSMLKSER + Oxidation (M) 761 536.2244 2140.8684 2140.9625 -43.96 1 9 5.6 1 MDAGIICDVCTCELQKEVR + Oxidation (M) 129 430.2237 858.4328 858.4130 23.1 1 9 11 1 THCSGRK + Propionamide (C) 438 623.3304 1244.6463 1244.6262 16.2 1 9 8.2 1 GQNPGDLRFNK 422 614.3615 1226.7085 1226.6255 67.7 0 9 7.9 1 APSSLSDAVPQR 168 439.2000 876.3854 876.4197 -39.11 1 9 9.7 1 DIACKCPK 468 429.1000 1284.2782 1284.4855 -161.38 0 9 4.4 1 YDEGSGGSGDEGR 359 547.2000 1092.3854 1092.5485 -149.22 0 9 8.2 1 ISSELEMLR + Oxidation (M) 292 494.3059 986.5972 986.4855 113 1 9 9.4 1 MTSTTRYK 149 435.2105 868.4064 868.4337 -31.52 0 9 7.1 1 QHLACAR + Propionamide (C) 291 493.1896 984.3647 984.6331 -272.67 1 9 8.3 1 QKSLLVVAK 478 432.1000 1293.2782 1293.5516 -211.36 0 9 5.3 1 CQVGAGMCPVDK + Oxidation (M); Propionamide (C) 693 451.3000 1801.1709 1800.9114 144 1 9 7.3 1 AAVTMFSSVMRLELSK + 2 Oxidation (M) 351 539.3000 1076.5854 1076.5325 49.2 0 9 9.2 1 YPPTVMQNK 509 441.2000 1320.5782 1320.6925 -86.57 0 9 8.2 1 NLTFQLESLEK 774 551.2402 2200.9318 2201.0463 -52.03 0 9 5.7 1 DPNKPLTYENMDVLFSFR + Oxidation (M) 701 453.2911 1809.1355 1808.9290 114 1 9 7 1 KPLCNWVRMVAAEHR 221 454.2363 906.4580 906.4613 -3.61 1 9 10 1 GRWFWR 650 429.1000 1712.3709 1712.8127 -257.91 1 9 7.9 1 WQYKLAACSVSCGR + 2 Propionamide (C) 253 470.2102 938.4059 938.5661 -170.72 1 9 8.1 1 HTISVRVK 686 449.0000 1791.9709 1791.9149 31.3 1 8 8.1 1 SSMAGKAVAITQSPSSVR + Oxidation (M) 640 425.2000 1696.7709 1696.9156 -85.30 1 8 7.4 1 LKLRPLYQFMSCK + Propionamide (C) 442 417.1000 1248.2782 1248.5346 -205.38 0 8 6.2 1 CSFSPWTEHR 777 555.1475 2216.5609 2217.2005 -288.46 1 8 11 1 LELEAYAGVISALRAQGDLTK

Downloaded from iovs.arvojournals.org on 10/02/2021 14 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

108 425.7692 849.5238 849.3837 165 1 8 11 1 CPMERK + Oxidation (M); Propionamide (C) 83 421.2116 840.4086 840.4202 -13.78 0 8 7.6 1 AGPGAAGAGGR 822 1215.9743 2429.9341 2430.1534 -90.22 0 8 7.5 1 TLWSCYLCCLLTAAAGAASYPPR 682 445.3000 1777.1709 1776.8215 197 0 8 8.3 1 DFWMNMQAVTVYLK + 2 Oxidation (M) 723 471.1000 1880.3709 1879.8379 284 1 8 10 1 VHSGEKPNKCMFEGCSK 729 970.9728 1939.9310 1939.9639 -16.97 1 8 6.6 1 GNEYEALTSPQTSFRLK 840 659.2559 2632.9943 2633.2755 -106.78 0 8 8.7 1 MNPALGNQTDVAGLFLANSSEALER + Oxidation (M) 304 501.3030 1000.5915 1000.5917 -0.20 1 8 10 1 LAKADGIVSK 631 415.1000 1656.3709 1656.7687 -240.10 0 8 7.1 1 WTHLPCGCIINCR + 2 Propionamide (C) 134 432.1331 862.2517 862.4218 -197.24 0 8 10 1 CAAQTLEK 599 513.2671 1536.7795 1536.7857 -4.05 1 8 7.7 1 IMALEKLEDAFNK + Oxidation (M) 541 452.3000 1353.8782 1353.7187 118 1 8 8.5 1 MLRAGAPTGDLPR 42 411.1000 820.1854 820.3497 -200.26 0 8 7 1 NMEAGAGR + Oxidation (M) 246 467.3000 932.5854 932.4538 141 0 8 11 1 LCHNVGYK 23 405.1456 808.2767 808.4120 -167.29 0 8 11 1 DVFFGPK 333 525.2000 1048.3854 1048.4574 -68.60 0 8 10 1 YEPAGGDANR 806 587.3586 2345.4055 2345.0933 133 1 8 8.2 1 LPIGDVATQYFADRDMFCAGR 678 441.2000 1760.7709 1760.7359 19.9 0 8 7.6 1 NHSGERPYPCNECGK + Propionamide (C) 295 496.7993 991.5841 991.6291 -45.30 1 8 10 1 RAALLVPPR 223 455.2208 908.4270 908.4394 -13.68 1 8 9.5 1 CLAMGMKR 356 544.2966 1086.5787 1086.6648 -79.25 1 8 11 1 EISISIIGKK 224 456.2205 910.4264 910.4616 -38.69 1 8 8.6 1 TGKMLMSK + Oxidation (M) 566 469.3000 1404.8782 1404.6959 130 0 8 8.8 1 SIVEELCAWVEK 203 450.2287 898.4429 898.4694 -29.55 1 8 8.7 1 MEPPAAKR 474 645.3339 1288.6532 1288.6809 -21.47 1 8 10 1 KAMLQEIANQK + Oxidation (M) 799 583.2800 2329.0909 2329.1203 -12.64 1 8 5.7 1 CGQAWGTMMVHKGLDLPCLK + 2 Propionamide (C) 58 415.2122 828.4098 828.4276 -21.46 1 8 11 1 KEMHLR + Oxidation (M) 98 424.1719 846.3293 846.4963 -197.37 0 8 12 1 ILVPSYR 819 603.2807 2409.0939 2409.1205 -11.08 1 8 5.7 1 GYMEHPDQTRTGFGCESLLLR 520 668.3376 1334.6607 1334.7962 -101.53 1 8 9.1 1 AIKIFLGGFELK 193 446.2669 890.5192 890.5338 -16.32 1 8 12 1 AVAYAKLR 535 451.1000 1350.2782 1350.6635 -285.32 1 8 5.3 1 TVLQMDELKCR + Oxidation (M) 592 500.2868 1497.8386 1497.6808 105 0 8 8.1 1 GVPAGNSDTEGGQPGR 855 721.4000 2881.5709 2881.4709 34.7 0 8 7.9 1 QQQLEEEAAKPPEPEKPVSPPPIESK 112 426.2319 850.4493 850.3603 105 0 8 9.7 1 MENQGTR + Oxidation (M) 639 425.2000 1696.7709 1696.9202 -87.97 1 8 8.4 1 IHRWTILHYSPFK 220 453.2911 904.5677 904.4436 137 0 8 12 1 AGAMPISSR + Oxidation (M) 524 446.2589 1335.7549 1335.6030 114 0 8 9.3 1 IYAMHWGTDSR 770 721.4000 2161.1782 2160.9894 87.4 0 8 6.4 1 LCYGLNMDFVDPAQITMK + 2 Oxidation (M); Propionamide (C) 344 535.2675 1068.5204 1068.4368 78.2 0 8 9.6 1 APDMGFEMR + Oxidation (M) 743 519.1415 2072.5368 2071.9588 279 1 8 13 1 DLMNTKSQLEISTQMCK + 2 Oxidation (M); Propionamide (C) 651 429.1000 1712.3709 1712.8015 -251.38 0 8 9.2 1 CNHTTNFAVLMTFK + Oxidation (M); Propionamide (C) 755 531.2128 2120.8223 2121.0421 -103.63 0 8 7.6 1 MQIMTHLPGAISDMIYIR + 2 Oxidation (M) 145 435.1000 868.1854 868.4153 -264.70 0 8 4.6 1 FIDPFCK 65 416.5801 831.1456 831.3909 -295.06 0 8 2.1 1 AQMVDPR + Oxidation (M) 334 525.2465 1048.4785 1048.5124 -32.32 0 8 11 1 GPLGQGAMFR + Oxidation (M) 74 421.1883 840.3620 840.4453 -99.13 1 8 8.9 1 DLHSKNK

Downloaded from iovs.arvojournals.org on 10/02/2021 15 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

498 437.1000 1308.2782 1308.6496 -283.83 1 8 5.9 1 QESVGVFACKNK 563 467.3000 1398.8782 1398.7765 72.7 1 8 9.5 1 CLRANQVLLQNK 544 453.2126 1356.6159 1356.7112 -70.18 1 8 10 1 TFMQGEFLKIK + Oxidation (M) 749 521.1157 2080.4335 2079.8774 267 1 8 12 1 CECYPGYEMDLLTKNCK + Propionamide (C) 793 577.2000 2304.7709 2305.0150 -105.91 1 8 11 1 SHMRTHTGEKPYVCQECGR + Oxidation (M); Propionamide (C) 172 441.2000 880.3854 880.4007 -17.34 1 8 10 1 ASSMCRR + Propionamide (C) 388 583.2983 1164.5820 1164.5887 -5.74 1 8 11 1 LYSENDRLR 552 691.3859 1380.7573 1380.6820 54.6 1 8 10 1 SMSAPVIFDRSR + Oxidation (M) 721 470.2636 1877.0252 1877.0047 10.9 1 7 8.5 1 GTASATKWAFVLASAAPTK 829 625.3255 2497.2730 2497.0867 74.6 1 7 5.9 1 AAVLFQPCGHMCACENCANLMKK + Oxidation (M) 733 984.0018 1965.9890 1966.0816 -47.09 0 7 8.2 1 FNFLSIDLPVVVETFVK 768 541.2484 2160.9646 2160.8775 40.3 0 7 7.1 1 CQGESGEWIACHEAPGGTCR + Propionamide (C) 117 427.2892 852.5639 852.4930 83.2 1 7 10 1 APVTPRGR 477 431.2093 1290.6061 1290.6350 -22.36 1 7 11 1 RISAEGSQACAK + Propionamide (C) 114 426.2436 850.4727 850.3603 132 0 7 11 1 MENQGTR + Oxidation (M) 264 475.3033 948.5921 948.4698 129 1 7 13 1 ALQKDGSCK 522 668.3621 1334.7096 1334.6289 60.4 0 7 11 1 GDSGGQGLWVTMK 503 439.1000 1314.2782 1314.5926 -239.18 1 7 6.2 1 HTDGHRHGENR 849 699.3715 2793.4569 2793.1576 107 1 7 7.6 1 EPWNMKICEMVDESPAMCSSFTR + 2 Oxidation (M); Propionamide (C) 718 469.2000 1872.7709 1872.8999 -68.90 1 7 9 1 KLGQIPEGEGASEQGMAR + Oxidation (M) 196 448.2556 894.4967 894.4447 58.1 0 7 12 1 FTDGITNK 55 414.7708 827.5271 827.4501 93.1 0 7 12 1 AVAEPVSR 386 581.2802 1160.5459 1160.5495 -3.14 1 7 12 1 KMEPDEQLR + Oxidation (M) 204 450.2508 898.4870 898.4079 88.1 0 7 11 1 AVHQCDR + Propionamide (C) 128 430.1000 858.1854 858.4130 -265.10 1 7 5.1 1 THCSGRK + Propionamide (C) 202 450.1000 898.1854 898.3789 -215.38 0 7 4 1 AFQGCMR + Oxidation (M); Propionamide (C) 26 406.2184 810.4223 810.4024 24.5 0 7 10 1 AAFFAER 272 480.2496 958.4847 958.5157 -32.34 1 7 14 1 LEKMTPPK + Oxidation (M) 462 427.2892 1278.8459 1278.5229 253 0 7 11 1 MCEPCGGLCPK + 2 Propionamide (C) 72 421.1844 840.3543 840.4382 -99.84 0 7 10 1 DVFSFVK 641 425.2000 1696.7709 1696.8818 -65.35 1 7 10 1 DTILHDNLKQLMLQ + Oxidation (M) 164 439.1000 876.1854 876.3694 -209.94 1 7 7 1 CPREGCGR 514 443.3678 1327.0814 1326.7143 277 0 7 4.5 1 SLPQSVIENVGGK 466 428.7768 1283.3084 1283.5850 -215.42 1 7 8.2 1 EATMDAVMTRK + 2 Oxidation (M) 557 462.3000 1383.8782 1383.5911 207 1 7 12 1 GQCFCDGAKGEK + 2 Propionamide (C) 573 710.4205 1418.8265 1418.6587 118 1 7 11 1 CWSLYGRCYLR 18 403.2000 804.3854 804.4164 -38.52 0 7 15 1 ALVEMAR + Oxidation (M) 289 491.2397 980.4649 980.5767 -113.98 0 7 12 1 TTLAHVIAR 812 593.1760 2368.6750 2369.1420 -197.15 1 7 15 1 DYIQSTMETEKTPQGLSNLAK + Oxidation (M) 262 474.7693 947.5240 947.4196 110 0 7 15 1 ASNEDGDIK 660 432.4000 1725.5709 1725.6619 -52.74 1 7 10 1 CGKAFNECSCFTDCK + Propionamide (C) 548 686.3950 1370.7754 1370.6612 83.3 0 7 11 1 TMKPHAELEGSR + Oxidation (M) 747 519.1479 2072.5626 2073.1582 -287.30 1 7 15 1 LTTDLQNTFGQLQLRVVK 287 490.2924 978.5703 978.4804 91.9 0 7 13 1 NVGMTVSQK + Oxidation (M) 596 504.2000 1509.5782 1509.7358 -104.41 1 7 11 1 VGNMYVRGLSGGER + Oxidation (M) 795 577.2660 2305.0348 2305.0604 -11.13 1 7 7.7 1 QAPSTELSTSGSSHSKSCDQLR 92 421.7710 841.5274 841.4770 59.9 1 7 12 1 IPNSQRK

Downloaded from iovs.arvojournals.org on 10/02/2021 16 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

687 449.0000 1791.9709 1791.9995 -15.97 1 7 12 1 EPALPPRPLDKYQLR 730 487.2117 1944.8176 1944.8498 -16.56 1 7 9.4 1 NSWGEEWGMGGYVKMAK + Oxidation (M) 401 599.3231 1196.6316 1196.5352 80.6 1 7 12 1 EKEVTMCMR + Propionamide (C) 421 409.9029 1226.6870 1226.6805 5.28 1 7 12 1 KIPNIGNVMNK 610 401.1000 1600.3709 1600.6965 -203.42 0 7 9.6 1 DVDQETGEDLNPNR 654 430.1000 1716.3709 1715.9280 258 0 7 12 1 DAAAFLLSCQIPGLVK + Propionamide (C) 787 567.2540 2264.9869 2265.1861 -87.92 0 7 8.3 1 QEMSPAGVPLLGMQLNEVKPK 720 469.3000 1873.1709 1872.7878 205 0 7 10 1 MDLMIDCQPPAMSYR + 2 Oxidation (M); Propionamide (C) 399 593.3212 1184.6278 1184.6554 -23.24 0 7 15 1 LPSLFPDQLR 538 451.3000 1350.8782 1350.6424 175 0 7 12 1 GSFPGVMPMIQR + 2 Oxidation (M) 630 415.1000 1656.3709 1656.8444 -285.81 1 7 10 1 SHLSRHQQTHIGEK 763 537.1843 2144.7082 2144.2027 236 1 7 13 1 LANFGGLAVGLGLGVLAEMAKK + Oxidation (M) 810 593.1714 2368.6565 2369.0748 -176.58 1 7 16 1 MSCQQNQQQCQPPPKCPIPK + Oxidation (M); Propionamide (C) 188 445.2243 888.4340 888.4963 -70.18 1 7 17 1 ACTLGRIR 815 594.1741 2372.6674 2373.0716 -170.35 0 7 16 1 MQEAMTQEVSDVFSDTTTPIK + Oxidation (M) 534 450.9115 1349.7126 1349.6398 54.0 0 6 13 1 LNQCWTTTQGK + Propionamide (C) 455 425.2000 1272.5782 1272.5881 -7.77 1 6 14 1 MSEPQPRGAER + Oxidation (M) 752 527.2127 2104.8218 2104.8361 -6.76 1 6 11 1 DDSLVCDCRHNTAGPECDR 150 435.2164 868.4182 868.4039 16.5 0 6 12 1 QQSTSYR 231 460.2343 918.4539 918.4957 -45.45 0 6 17 1 VLMGGTLGR + Oxidation (M) 243 466.2470 930.4795 930.4957 -17.32 1 6 16 1 IPNKAMNK + Oxidation (M) 499 437.1388 1308.3947 1308.6496 -194.82 1 6 13 1 QESVGVFACKNK 94 423.2411 844.4676 844.4039 75.4 1 6 18 1 DKGHSSSK 298 498.2406 994.4666 994.5196 -53.24 0 6 14 1 VHSGEKPNK 769 541.2506 2160.9734 2160.9799 -3.01 1 6 8.9 1 QYEINATPKGWSCHWDR + Propionamide (C) 844 669.3677 2673.4415 2674.2223 -291.97 1 6 9.5 1 TINCSCPAGEKGEMGVAGPMGLPGPK + 2 Oxidation (M); 2 Propionamide (C) 86 421.2194 840.4242 840.5069 -98.42 0 6 12 1 EAVVAKPK 574 476.2608 1425.7606 1425.6666 65.9 1 6 12 1 MAGIATMEEVKCK + Oxidation (M) 649 856.4035 1710.7925 1710.9048 -65.64 0 6 11 1 VLLSVMYLMVENIR + 2 Oxidation (M) 212 451.3000 900.5854 900.5028 91.7 1 6 18 1 IKENELR 839 656.3684 2621.4447 2622.2280 -298.74 1 6 10 1 LYHGIMMESFMRDDIIYSTLR + 2 Oxidation (M) 745 519.1479 2072.5626 2071.9952 274 1 6 17 1 SELLMIIQMETQSSMRK + 3 Oxidation (M) 430 619.2816 1236.5486 1236.5193 23.7 0 6 13 1 QYEANPACEGR 670 439.1000 1752.3709 1752.8426 -269.13 0 6 13 1 VTCVEMEPLAEYVVR + Oxidation (M) 772 543.2000 2168.7709 2169.0643 -135.27 1 6 12 1 YFNILTTQWEQKYHGNK 21 404.2340 806.4535 806.4147 48.1 0 6 16 1 AQHPQAR 467 428.8904 1283.6493 1283.5663 64.7 0 6 13 1 MDSIGSTISSDR + Oxidation (M) 738 507.2000 2024.7709 2024.9871 -106.75 1 6 10 1 MGSSTSEKTLNICEVGTIR 741 517.2205 2064.8530 2065.0626 -101.53 1 6 9.8 1 SQSVRPGADVTFICTAKSK + Propionamide (C) 659 432.1000 1724.3709 1724.7974 -247.28 1 6 14 1 LDVSFQRACQCEGK + 2 Propionamide (C) 719 469.2000 1872.7709 1872.9093 -73.92 1 6 11 1 FPGIWAHGDYCRINPK 155 435.2381 868.4616 868.3354 145 0 6 12 1 MCTGGCAR + Propionamide (C) 494 435.1000 1302.2782 1302.5914 -240.48 0 6 8.6 1 SEQFMIYNQK + Oxidation (M) 267 477.2234 952.4322 952.5202 -92.42 1 6 14 1 TANRNHLK 284 486.2808 970.5470 970.4066 145 0 6 15 1 YPSTCNVTS 707 462.1000 1844.3709 1844.7492 -205.05 0 6 16 1 QEMTSTVYCHGGGSCK + Oxidation (M); 2 Propionamide (C) 118 427.3000 852.5854 852.4090 207 0 6 13 1 THPGGEQK

Downloaded from iovs.arvojournals.org on 10/02/2021 17 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

111 426.2241 850.4337 850.4120 25.5 0 6 14 1 AVFGQCR + Propionamide (C) 496 654.3631 1306.7117 1306.6980 10.5 0 6 14 1 ITESTGAITSISK 314 507.2280 1012.4415 1012.4978 -55.63 0 6 14 1 GFSNFSNLK 726 642.3621 1924.0643 1923.9724 47.8 1 6 11 1 MTLDRSEYTSLIPNLR + Oxidation (M) 371 563.2908 1124.5671 1124.5318 31.4 1 6 15 1 CALTGQSKSCK 241 466.2062 930.3978 930.5208 -132.19 0 6 18 1 IPVTLNMK + Oxidation (M) 266 476.2298 950.4451 950.4644 -20.30 0 6 16 1 AMVVSSWR + Oxidation (M) 99 424.2362 846.4579 846.4269 36.5 0 6 19 1 VPQASAMK + Oxidation (M) 315 507.2621 1012.5096 1012.6141 -103.19 1 6 14 1 AAGLLSGLRR 811 593.1737 2368.6657 2368.1475 219 1 6 18 1 CIRTLTSSGQVISGDACAATSTR + Propionamide (C) 192 446.2649 890.5152 890.4570 65.4 1 6 19 1 TDSGSLRR 706 462.1000 1844.3709 1843.9251 242 0 6 17 1 NLGFTAIPLHGQMSQSK + Oxidation (M) 123 429.1000 856.1854 856.4337 -289.90 1 6 5.8 1 CSNKHLR 625 411.2000 1640.7709 1640.7768 -3.59 1 6 13 1 FIHGHRGGSGSGSGGSGK 618 409.2000 1632.7709 1632.9199 -91.24 0 6 13 1 AALPSLTNPPSLSPIR 32 408.2325 814.4505 814.5025 -63.82 1 6 18 1 LGGTLARK 679 443.1000 1768.3709 1768.8600 -276.51 1 6 16 1 SREAVAQAMVLEMFR + 2 Oxidation (M) 40 410.2344 818.4542 818.4181 44.1 1 6 20 1 MARAAGAR + Oxidation (M) 757 536.1652 2140.6318 2139.9982 296 1 6 19 1 IHNGEKPYACEACQKFFR 832 631.3418 2521.3380 2521.1927 57.6 0 6 10 1 TEEMLSNQMLSGIPEVDLGIDAK + 2 Oxidation (M) 338 530.2800 1058.5455 1058.5396 5.52 0 6 17 1 APETEPFLR 59 415.2180 828.4214 828.4705 -59.33 0 6 19 1 VPQSGTLK 90 421.2232 840.4319 840.3833 57.8 1 6 14 1 SCKSSCVK 148 435.2085 868.4024 868.3030 114 0 6 13 1 HCQTFCM 725 473.1000 1888.3709 1887.9295 234 1 6 18 1 TNMTGMAGLAGLKHDIAR + 2 Oxidation (M) 526 447.3000 1338.8782 1338.6523 169 0 6 15 1 LLASCSADMTIK + Oxidation (M); Propionamide (C) 564 469.2000 1404.5782 1404.8089 -164.25 0 6 15 1 HGVPISVTGIAQVK 775 551.2913 2201.1361 2201.1523 -7.36 1 6 11 1 AVLVVLGPWCADKVAGMMVR + Oxidation (M); Propionamide (C) 782 558.2099 2228.8106 2229.2092 -178.80 0 6 13 1 IPVPILPGLPMNNHGNYIVR + Oxidation (M) 813 593.1806 2368.6934 2369.1070 -174.58 1 6 19 1 GGDAASHLFTASLGGKNQYSSCK + Propionamide (C) 801 585.2718 2337.0580 2337.2726 -91.81 1 6 10 1 QEKPERLLQTLAPQAMLVEK + Oxidation (M) 78 421.2077 840.4009 840.4130 -14.44 0 6 14 1 LDAGFYR 357 545.3212 1088.6279 1088.6342 -5.81 1 6 18 1 GIQLFDRIK 469 429.1000 1284.2782 1284.4855 -161.38 0 6 8.7 1 YDEGSGGSGDEGR 299 498.2913 994.5680 994.5157 52.6 0 6 16 1 AIYELQMK 652 571.8644 1712.5713 1712.8266 -149.04 1 6 14 1 MPFSVYSTLEKHMK + Oxidation (M) 788 569.2702 2273.0515 2272.9084 63.0 0 6 11 1 CDDMLTCYMFHMYVGVR + 3 Oxidation (M); 2 Propionamide (C) 847 697.3415 2785.3367 2785.2904 16.6 1 6 9.4 1 AAQSGDYGGAGDDYVLGSLRVSGSGGGGAGK 308 503.2664 1004.5182 1004.5654 -47.06 0 6 19 1 LLAADALYR 644 427.2000 1704.7709 1704.8180 -27.61 1 6 14 1 GEPGPQGSAGQRGEPGPK 68 417.2175 832.4205 832.3926 33.4 1 6 20 1 EKDAENK 345 535.2718 1068.5291 1068.4766 49.2 0 6 16 1 ASMCVLQCK + Oxidation (M); Propionamide (C) 180 443.1000 884.1854 884.4392 -286.92 0 6 6 1 WHTLLES 568 471.1000 1410.2782 1410.5859 -218.14 0 6 7.5 1 SHSSGSSSSSSSEAK 66 417.1000 832.1854 832.4151 -275.92 1 6 12 1 KGQGSGGSR 683 447.3000 1785.1709 1784.8733 167 0 6 14 1 NPDQVYLVDYGLAYR 595 753.4091 1504.8036 1504.8357 -21.30 1 6 16 1 ILLAAMCLVTKGEK + Oxidation (M)

Downloaded from iovs.arvojournals.org on 10/02/2021 18 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

606 529.2479 1584.7219 1584.7766 -34.51 1 6 14 1 KHLHNCHIPGCGK + 2 Propionamide (C) 109 425.7692 849.5238 849.4709 62.3 0 5 21 1 SLTAGVFR 130 430.3120 858.6095 858.4810 150 0 5 22 1 AAGSLLAEK 756 535.2303 2136.8919 2137.0877 -91.62 1 5 11 1 LAQGEYIAPEKIENIYMR 309 504.2000 1006.3854 1006.4614 -75.49 1 5 18 1 AGGKTGNGCSR 335 525.2726 1048.5306 1048.5587 -26.84 1 5 19 1 EVVMRVTTV + Oxidation (M) 809 593.1691 2368.6473 2368.0778 240 1 5 21 1 KPEEEGSAHQDTAEKNASSPEK 671 439.1000 1752.3709 1751.8494 298 1 5 16 1 YFGGMVWYFVNNKK 767 541.2044 2160.7886 2161.2180 -198.71 0 5 13 1 LLPIGLNICAPGDQELIALAK 349 538.2900 1074.5655 1074.5604 4.72 1 5 20 1 RSVVEAMQR 740 514.1720 2052.6588 2052.1004 272 1 5 16 1 YLGTGKSTHGLAILGPENPK 378 574.3217 1146.6288 1146.5703 51.0 0 5 19 1 MADSSPALSLR 497 436.8976 1307.6708 1307.6584 9.51 0 5 17 1 YLPGPLQDMFK 235 462.1000 922.1854 922.4364 -272.09 1 5 9.5 1 ALCADMKR + Oxidation (M) 436 415.2180 1242.6320 1242.6833 -41.22 1 5 17 1 SSRHLAAFINK 817 596.3161 2381.2351 2381.0892 61.3 0 5 10 1 MAFAETYPAASSLPNGDCGRPR + Propionamide (C) 845 681.3579 2721.4026 2721.3445 21.3 1 5 11 1 ICREGNGVSVSWRPSVEFPGNLYR 547 453.5772 1357.7098 1357.7751 -48.13 1 5 17 1 AICRSVLENLIK 838 651.3705 2601.4531 2602.1462 -266.35 1 5 14 1 CDVCNYSTTTKGNLSIHMQSDK + Oxidation (M); 2 Propionamide (C) 657 430.9000 1719.5709 1719.9169 -201.16 1 5 16 1 LLHIDFRDAVAHASR 384 580.3181 1158.6216 1158.6132 7.27 0 5 19 1 ETLPISSTPSK 139 433.1000 864.1854 864.3760 -220.41 1 5 11 1 MREDGGGK + Oxidation (M) 572 473.1000 1416.2782 1416.6845 -286.81 0 5 8.3 1 TSQVQGELGDDLR 33 409.1000 816.1854 816.3324 -179.99 0 5 10 1 GVYMSGST + Oxidation (M) 89 421.2232 840.4319 840.3436 105 0 5 16 1 DPYGSMR + Oxidation (M) 146 435.2045 868.3945 868.4113 -19.29 1 5 16 1 ECGKAFSK 339 530.2887 1058.5629 1058.4525 104 1 5 20 1 SKSCEFCGK + Propionamide (C) 435 415.1000 1242.2782 1242.5881 -249.39 0 5 11 1 YGVEAFSDSLR 479 432.2000 1293.5782 1293.6599 -63.13 0 5 18 1 LSTMETGTGLIR + Oxidation (M) 470 643.3071 1284.5997 1284.6422 -33.10 0 5 18 1 NAAAQGSPEATLR 791 571.2514 2280.9767 2281.1413 -72.15 1 5 13 1 FELVHIDTKSATQMFELTR + Oxidation (M) 265 475.7345 949.4544 949.4869 -34.21 0 5 21 1 DYLEAAIR 711 462.3000 1845.1709 1845.7201 -297.58 1 5 14 1 CHGMSGSCTVRTCWMR + 2 Oxidation (M) 73 421.1844 840.3543 840.4705 -138.30 0 5 16 1 ALIGPDQK 705 459.3000 1833.1709 1832.9342 129 1 5 17 1 VIILMDPFDDDLKQR + Oxidation (M) 665 435.1000 1736.3709 1735.9001 271 0 5 18 1 QMLEVILAIGNFMNK + Oxidation (M) 161 437.2931 872.5717 872.3521 252 0 5 25 1 MEMFQR + 2 Oxidation (M) 449 421.1000 1260.2782 1260.6397 -286.79 0 5 12 1 MTSSLQRPWR 143 434.7685 867.5224 867.4636 67.7 0 5 18 1 LSPCLHK + Propionamide (C) 525 670.3299 1338.6453 1338.7143 -51.56 1 5 18 1 KQEVDSAPQLPK 805 587.2738 2345.0661 2344.9949 30.4 1 5 13 1 FGEAPMHFCEVMLKDMADSR + 2 Oxidation (M) 337 529.2871 1056.5596 1056.5274 30.5 1 5 20 1 SSTMRISYL 669 437.2674 1745.0405 1744.8744 95.2 0 5 15 1 EEPAVQFNSGTLALNR 533 450.1000 1347.2782 1347.6024 -240.56 0 5 12 1 MAAHTMGNATVGR + 2 Oxidation (M) 332 524.2735 1046.5324 1046.5794 -44.89 1 5 22 1 AMAEGKITVK 766 539.3000 2153.1709 2152.9946 81.9 0 5 14 1 SEIIPMFSNLASDEQDSVR + Oxidation (M) 460 427.1000 1278.2782 1278.6489 -289.97 0 5 12 1 LMNSQLVTTEK + Oxidation (M)

Downloaded from iovs.arvojournals.org on 10/02/2021 19 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

555 462.1000 1383.2782 1383.6354 -258.15 0 5 10 1 ADHHQQMFNIK + Oxidation (M) 174 441.2011 880.3877 880.3749 14.5 0 5 20 1 HTSMYDK 363 552.3136 1102.6126 1102.5407 65.2 0 5 23 1 ITVESDHFR 381 576.3140 1150.6134 1150.5288 73.5 1 5 21 1 KCETQLSQGS + Propionamide (C) 279 483.2252 964.4358 964.4284 7.65 0 5 24 1 MVDSGTEAR 395 589.3551 1176.6956 1176.6979 -1.98 1 5 23 1 THILTHVKTK 620 547.2000 1638.5782 1638.7031 -76.24 0 5 17 1 WYMHSCSGSLQNR + Propionamide (C) 152 435.2203 868.4261 868.5243 -113.02 1 5 18 1 LLQPSRR 676 441.1000 1760.3709 1760.8403 -266.57 1 5 22 1 YLQAEEYSIRSCTK + Propionamide (C) 848 697.3989 2785.5665 2786.3918 -296.16 1 5 19 1 SLPQVCPGPAMAKTPPQMHPVTTPAK + 2 Oxidation (M); Propionamide (C) 452 633.3663 1264.7180 1264.5904 101 1 5 20 1 ESCGLCLKADR + Propionamide (C) 69 418.2268 834.4391 834.3654 88.3 0 5 24 1 AQNSEMR 826 621.3480 2481.3628 2481.3088 21.8 1 5 14 1 EQASHLGGAVFSGAGNIAAATGLVKR 159 437.1000 872.1854 872.3988 -244.54 0 5 9.3 1 EGEGASAPR 504 439.1000 1314.2782 1314.5696 -221.70 1 5 12 1 AECTAEKCYR + 2 Propionamide (C) 432 413.2000 1236.5782 1236.6363 -47.03 0 5 21 1 AWADPATPRPR 124 429.1000 856.1854 856.2990 -132.59 0 4 8.3 1 CCSTGCGR + Propionamide (C) 296 497.2338 992.4531 992.4484 4.68 0 4 24 1 LVEDMENK + Oxidation (M) 804 587.2738 2345.0661 2344.9861 34.1 0 4 14 1 MLDEQDVPAAEQMCFETSAK + 2 Oxidation (M); Propionamide (C) 742 691.3859 2071.1359 2071.0078 61.9 0 4 15 1 TIEAAEALLHMESPTCLR + Oxidation (M); Propionamide (C) 15 400.2192 798.4239 798.3409 104 0 4 23 1 AHDWDR 82 421.2096 840.4047 840.4454 -48.32 1 4 19 1 SDHGGIKK 464 641.3075 1280.6004 1280.7088 -84.65 1 4 21 1 AGLVIPPEKSDR 600 513.2950 1536.8631 1536.7575 68.7 1 4 19 1 GIAACMTCGNTKVLR 461 427.2000 1278.5782 1278.6680 -70.27 1 4 22 1 IRPSKDSSSFR 808 593.1691 2368.6473 2368.0034 272 1 4 28 1 THTGEKPYECNECGKSFCHK + Propionamide (C) 827 625.2735 2497.0648 2497.1981 -53.38 1 4 14 1 YHSLGLFPMDSLAAEIEGTMRK + 2 Oxidation (M) 113 426.2378 850.4610 850.4661 -5.92 1 4 23 1 YRSALNK 214 452.3000 902.5854 902.5259 66.0 1 4 29 1 LKALEAMK 116 427.2000 852.3854 852.3218 74.7 0 4 21 1 CGESGACR + Propionamide (C) 797 582.3322 2325.2997 2325.2523 20.4 1 4 16 1 MPRAPAPLYACLLGLCALLPR + Oxidation (M); Propionamide (C) 735 500.2868 1997.1181 1997.0945 11.8 0 4 17 1 LHPEALNLSLDELPPALR 818 597.2844 2385.1086 2385.2904 -76.20 0 4 15 1 LQALLQDLSEVSAPPLPPTSPGR 746 519.1479 2072.5626 2072.0587 243 1 4 30 1 VITMYRDIFGMWVKPGK + 2 Oxidation (M) 614 814.9737 1627.9329 1627.8279 64.5 0 4 21 1 LYIIYNMLEVADR + Oxidation (M) 475 430.8931 1289.6574 1289.5454 86.9 0 4 23 1 EICCYSISCK + 2 Propionamide (C) 779 557.2224 2224.8606 2224.8556 2.26 1 4 18 1 CRCVFHWCCYVSCQECTR 311 505.2740 1008.5334 1008.4885 44.5 0 4 24 1 MVSWMISR 816 595.2031 2376.7831 2377.1954 -173.44 0 4 28 1 FSAGDVVEVLVPEAQNGWLYGK 846 691.3859 2761.5146 2761.5086 2.17 0 4 20 1 DVLLAATANTSSSQLAALLQLQHQLR 186 444.2367 886.4589 886.5123 -60.34 1 4 33 1 GLLKEAEK 63 416.2364 830.4583 830.4320 31.7 0 4 33 1 LEMPVAR + Oxidation (M) 628 413.2000 1648.7709 1648.9260 -94.08 1 4 22 1 ILHLQRVIDSQAEK 624 411.1000 1640.3709 1640.8482 -290.88 1 4 21 1 TNEAVPKTAPTNVSGR 831 629.3347 2513.3099 2513.4005 -36.08 1 4 15 1 LWAYLTINQLLAERSLAPTAAAK 668 437.1000 1744.3709 1744.7430 -213.27 1 4 26 1 MTMEKGMSSGEGLPSR + 3 Oxidation (M) 331 523.3121 1044.6097 1044.4836 121 1 4 29 1 DDSSPSPKGR

Downloaded from iovs.arvojournals.org on 10/02/2021 20 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

565 469.2000 1404.5782 1404.7150 -97.39 1 4 24 1 AVDFAERHGYIK 802 586.2357 2340.9138 2341.0265 -48.15 0 4 20 1 MHPFQWCNGCFCGLGLVSTNK 764 537.2041 2144.7872 2145.0711 -132.34 0 4 20 1 HCGNIAPPTIISSGSMLYIR + Oxidation (M) 633 416.2000 1660.7709 1660.8276 -34.15 1 4 22 1 SSIKIECVLPENCR + Propionamide (C) 794 577.2614 2305.0166 2305.1559 -60.41 1 4 17 1 YTQNDLMIDPLIVLRCDQR 751 525.2639 2097.0264 2097.0313 -2.35 1 4 18 1 AALSKDPGTGFVFSCLSGQGR 51 413.2000 824.3854 824.4392 -65.21 0 3 25 1 EPTTIHK 79 421.2077 840.4009 840.3041 115 0 3 24 1 CMAGGCGR + Oxidation (M); Propionamide (C) 390 584.3040 1166.5935 1166.5212 62.0 0 3 28 1 GDNWMLMLR + 2 Oxidation (M) 38 410.1826 818.3507 818.4572 -130.07 0 3 36 1 LMEIIGK + Oxidation (M) 271 479.2592 956.5038 956.4675 37.9 1 3 30 1 DQPQEGRK 783 558.2658 2229.0342 2229.0479 -6.18 0 3 19 1 YSGSQMEILANQLTGLMDMK 47 412.1892 822.3639 822.3905 -32.39 0 3 29 1 AQVTSCSK 20 404.2169 806.4193 806.3480 88.4 0 3 32 1 TVAPTECS 131 430.9000 859.7854 859.5280 300 1 3 1.6 1 LPKSLFR 800 585.2214 2336.8566 2336.1756 292 1 3 26 1 YDIKCAVSEAAIILNSCVEPK + Propionamide (C) 781 557.2358 2224.9142 2225.1667 -113.46 1 3 20 1 WFSSVLASCPKKPVSSYLR + Propionamide (C) 553 692.3265 1382.6385 1382.6864 -34.62 0 3 28 1 SILDQSISSFMR 758 536.1762 2140.6756 2141.0258 -163.56 1 3 32 1 MWEPPLRPAASCRGQAER + Oxidation (M); Propionamide (C) 175 441.2051 880.3956 880.5018 -120.61 0 3 29 1 TASPPALPK 473 430.1000 1287.2782 1287.5765 -231.70 0 3 16 1 YSLGSSLDSGMR + Oxidation (M) 684 596.3161 1785.9263 1785.8793 26.3 1 3 25 1 LILFTSAECHYSMKK + Oxidation (M) 28 406.2356 810.4566 810.3615 117 0 3 26 1 IMQEMK + 2 Oxidation (M) 305 501.8051 1001.5957 1001.4712 124 1 3 35 1 MAENREPR 119 428.1932 854.3718 854.4399 -79.65 0 3 25 1 AHAGPGAFK 493 435.1000 1302.2782 1302.5775 -229.82 0 3 18 1 VTWDGHSGSMAR 138 433.1000 864.1854 864.4011 -249.49 1 3 19 1 KIGDSCDK 482 432.8886 1295.6441 1295.7132 -53.31 1 3 29 1 APRCLSRPTAPK 71 421.1000 840.1854 840.4202 -279.34 0 3 12 1 AGPGAAGAGGR 190 445.3000 888.5854 888.4454 158 1 3 40 1 HSFGKEGK 281 484.2759 966.5373 966.4514 88.9 0 3 32 1 CLSVDCSLK 834 638.3641 2549.4272 2549.2406 73.2 1 3 22 1 CEICKQPLENLQAGDSIWIYR + Propionamide (C) 209 451.3000 900.5854 900.3977 208 0 3 40 1 FASEEYR 302 500.2868 998.5591 998.5509 8.20 1 3 33 1 LGRDSPINK 96 423.2703 844.5260 844.5031 27.0 0 3 42 1 HLHAVIR 218 453.2106 904.4066 904.4623 -61.53 0 3 38 1 ACIAGLMR + Propionamide (C) 165 439.1000 876.1854 876.4236 -271.68 1 3 19 1 MAEGARAR + Oxidation (M) 237 462.1000 922.1854 922.4508 -287.70 0 3 17 1 NEGAGGPPPK 239 464.1433 926.2721 926.4861 -231.02 0 3 26 1 YLNNLYK 760 536.1762 2140.6756 2140.9856 -144.79 1 3 35 1 VPCLNSDFGCPFTMARNK + 2 Propionamide (C) 369 557.3028 1112.5911 1112.5437 42.6 0 3 32 1 CLYVFGGQR + Propionamide (C) 762 537.1756 2144.6732 2145.0809 -190.10 1 3 35 1 NLGLTPMDQGSLMAAKEIEK 120 428.2030 854.3914 854.3592 37.6 0 3 28 1 AFEECTR 41 411.1000 820.1854 820.3749 -230.92 0 3 26 1 EMGDLTR 31 408.2058 814.3970 814.4297 -40.19 0 2 40 1 AQLGNGQK 87 421.2194 840.4242 840.5797 -184.98 1 2 30 1 AAKVVILK 22 404.2359 806.4573 806.3406 145 0 2 38 1 DATSDNGK

Downloaded from iovs.arvojournals.org on 10/02/2021 21 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

269 477.2668 952.5190 952.4688 52.7 0 2 33 1 ELFSQVCK 77 421.2058 840.3970 840.4276 -36.43 1 2 30 1 RVMTYR + Oxidation (M) 110 426.2163 850.4181 850.4297 -13.68 1 2 35 1 AFRESNK 142 434.6955 867.3764 867.3909 -16.63 0 2 34 1 AFAMEQR + Oxidation (M) 191 446.2609 890.5072 890.4280 89.0 1 2 44 1 DGPRATMK + Oxidation (M) 851 711.3715 2841.4569 2841.5314 -26.22 0 2 24 1 VAVIASIVSCAIILLMSMAFLTCCLLK + Oxidation (M) 151 435.2203 868.4261 868.4113 17.1 0 2 31 1 YCNATVK + Propionamide (C) 230 459.3000 916.5854 916.4978 95.7 1 2 48 1 KPKSSENK 632 415.2180 1656.8427 1656.7791 38.4 1 2 32 1 DGATAMHFAASRGHTK 521 668.3401 1334.6655 1334.6765 -8.20 0 2 38 1 GNFLQSCVQLR + Propionamide (C) 95 423.2566 844.4987 844.4403 69.2 0 2 53 1 LPAGTSGSR 147 435.2045 868.3945 868.4113 -19.29 0 2 34 1 YCNATVK + Propionamide (C) 207 451.1727 900.3308 900.4123 -90.55 0 2 52 1 MDPPAGAAR + Oxidation (M) 294 496.1817 990.3489 990.5359 -188.83 1 2 46 1 AGSQGLRFR 85 421.2155 840.4164 840.3470 82.6 0 2 37 1 MMTAESR + Oxidation (M) 52 414.2104 826.4062 826.4773 -86.08 1 1 39 1 APRLGSAR 127 430.1000 858.1854 858.4130 -265.10 1 1 19 1 THCSGRK + Propionamide (C) 170 440.2279 878.4413 878.4102 35.4 0 1 52 1 AGALCAMR + Oxidation (M); Propionamide (C) 276 482.2233 962.4320 962.5106 -81.65 0 1 52 1 MTIAALESK 16 401.1000 800.1854 800.4028 -271.59 0 1 23 1 DTPVQNK 45 411.1815 820.3484 820.3385 12.1 0 1 52 1 CPSGDSAGK 238 462.3000 922.5854 922.4872 106 0 1 48 1 SSSPVPPPR 513 443.1000 1326.2782 1326.6173 -255.62 0 1 27 1 ATPVCCAGQGPPR + Propionamide (C) 244 467.2036 932.3925 932.3997 -7.64 0 1 57 1 CLCHSGWK 43 411.1000 820.1854 820.4047 -267.30 1 1 36 1 ALCKGCR + Propionamide (C) 75 421.1961 840.3776 840.4454 -80.69 0 1 41 1 GAPVGAVDR 157 435.7850 869.5554 869.4355 138 0 1 45 1 AGGAAAAPER 194 447.2527 892.4909 892.3861 117 0 1 54 1 CWSGNQK + Propionamide (C) 320 513.2950 1024.5754 1024.5917 -15.90 0 1 46 1 VNLSPVTPAK 484 433.1000 1296.2782 1296.6536 -289.54 0 1 27 1 APAIIYDCYLR 587 740.3835 1478.7525 1478.6428 74.2 1 1 46 1 CSGKAVAMMHQER + 2 Oxidation (M) 154 435.2361 868.4577 868.4402 20.1 1 1 43 1 EAHKQEK 27 406.2260 810.4375 810.5327 -117.42 0 1 44 1 AGKPVIVK 36 409.1850 816.3554 816.4090 -65.59 1 1 63 1 RAPDSGSK 205 450.2749 898.5352 898.4257 122 0 1 48 1 AEGAPNQGR 407 603.3365 1204.6585 1204.6274 25.8 0 0 58 1 RPDMALYVPK + Oxidation (M) 70 420.2103 838.4061 838.4371 -37.00 0 0 49 1 MLLFSGR + Oxidation (M) 236 462.1000 922.1854 922.4243 -258.99 0 0 30 1 ATTEADSTK 260 473.1000 944.1854 944.4320 -261.10 1 0 20 1 VHGCCRIS + Propionamide (C) 213 452.1056 902.1966 902.4610 -293.02 0 0 31 1 ANQLGWSK 255 471.1000 940.1854 940.4337 -264.02 0 0 22 1 WHGPAMAR + Oxidation (M) 93 422.2335 842.4524 842.5378 -101.33 0 0 63 1 LLPVVFR 199 449.2095 896.4045 896.4790 -83.00 1 0 50 1 YAVVKCSK 480 432.4000 1294.1782 1293.8132 282 1 0 7 1 QGGVIRLTIPIK 485 433.1000 1296.2782 1296.6244 -267.05 1 0 33 1 RNSYTNCLQK + Propionamide (C) 1 411.2889 410.2816 2 411.2985 410.2912

Downloaded from iovs.arvojournals.org on 10/02/2021 22 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

3 413.2925 412.2852 4 415.2141 414.2068 5 416.2210 415.2137 6 416.2210 415.2137 7 416.2828 415.2755 8 417.3547 416.3474 9 425.2774 424.2701 10 425.2949 424.2877 11 437.2041 436.1968 12 442.2947 441.2874 13 501.2077 500.2004 14 581.3829 580.3756 17 401.1000 800.1854 39 410.2286 818.4427 56 415.1000 828.1854 57 415.1000 828.1854 60 415.5708 829.1271 80 421.2096 840.4047 81 421.2096 840.4047 88 421.2213 840.4280 107 425.2188 848.4231 115 427.1000 852.1854 121 428.2715 854.5284 132 431.2093 860.4041 136 432.4000 862.7854 137 432.8847 863.7549 181 443.1885 884.3624 197 449.0000 895.9854 198 449.0000 895.9854 225 456.2487 910.4829 227 457.2577 912.5008 229 458.7984 915.5823 257 471.1000 940.1854 303 501.2924 1000.5703 382 577.2000 1152.3854 397 592.3353 1182.6561 434 415.1000 1242.2782 511 441.5708 1321.6905 515 443.7284 1328.1633 852 713.3486 2849.3652

Search Parameters

Type of search : MS/MS Ion Search Enzyme : Trypsin Variable modifications : Oxidation (M),Propionamide (C) Mass values : Monoisotopic

Downloaded from iovs.arvojournals.org on 10/02/2021 23 of 24 3/07/2012 3:06 PM Peptide Summary Report (Submitted from PROJ13988 by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120703/F077965.dat&REPT...

Protein Mass : Unrestricted Peptide Mass Tolerance : ± 300 ppm Fragment Mass Tolerance: ± 0.6 Da Max Missed Cleavages : 1 Instrument type : ESI-QUAD-TOF Number of queries : 856

Mascot: http://www.matrixscience.com/

Downloaded from iovs.arvojournals.org on 10/02/2021 24 of 24 3/07/2012 3:06 PM