Complete Blood Count
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Markers of Carotid Atherosclerosis in Type 2 Diabetes Mellitus? Riya M Waghale, Rajashree Sanjay Khot, Prashant P Joshi Impact of Smoking and Nicotine Addiction
2020, Vol. 9, No. 2 ISSN 2450–7458 Platelet volume indices: markers of carotid atherosclerosis in type 2 diabetes mellitus? Riya M Waghale, Rajashree Sanjay Khot, Prashant P Joshi Impact of smoking and nicotine addiction on HbA1c levels and diabetic microvascular complication Hüseyin Akkuzulu, Cenk Aypak, Ayşe Özdemir, Süleyman Görpelioğlu Sugary beverages consumption and latent autoimmune diabetes in adults: systematic review and meta-analysis Ahmed Mahmoud El-Malky, Ramachandra G Naik, Azza A Elnouman The effect of low dose glargine U 300 on uncontrolled type 2 diabetes mellitus. An observational study in Indian patients Asis Mitra, Saswati Ray, Sushma Jayan Coffee in the diet and prevention of diabetes Regina Wierzejska Editor-in-Chief Scientific Board dr hab. n. med. Leszek Czupryniak, prof. nadzw. (Poland) prof. Antionio Ceriello (Spain) prof. dr hab. n. med. Edward Franek (Poland) Deputy Editor-in-Chief prof. dr hab. n. med. Władysław Grzeszczak (Poland) prof. dr hab. n. med. Wojciech Młynarski (Poland) prof. Martin Haluzík (Czech Republic) prof. dr hab. n. med. Krzysztof Strojek (Poland) prof. dr hab. n. med. Przemysława Jarosz-Chobot (Poland) prof. Nebojsa Lalic (Serbia and Montenegro) Editorial Board prof. Pierre Lefebvre (Belgium) prof. dr hab. n. med. Katarzyna Cypryk (Poland) prof. dr hab. n. med. Maciej Małecki (Poland) prof. Larisa Danilova (Belarus) prof. dr hab. n. med. Andrzej Milewicz (Poland) prof. dr hab. n. med. Janusz Gumprecht (Poland) prof. dr hab. n. med. Dariusz Moczulski (Poland) prof. dr hab. n. med. Krzysztof Narkiewicz (Poland) prof. dr hab. n. med. Irina Kowalska (Poland) dr Katherine Owen (United Kingdom) prof. dr hab. n. med. -
Interpreting Your Child's Lab Results
www.ComplexChild.com Interpreting Your Child’s Lab Results When you get a list of labs back from your doctor or hospital, your eye is drawn immediately to the starred or highlighted results that came back abnormal. Knowing that any of your child’s results have come back abnormal can be disconcerting. Much of the time, however, there is no need to worry. But how do you know when abnormal results indicate a little problem, a big problem, or not a problem at all? Reference Ranges The first thing you need to do is look at the reference ranges that are given along with the results. Each lab has its own equipment that is calibrated uniquely, and results vary depending on the lab. While most tests have similar reference ranges from lab to lab, there are some, such as the test for Lipase, that use different testing systems with very different reference ranges. Results from one lab may not be equivalent to results at another lab. The Slightly High or Low Result In many cases, the abnormal result is ever so slightly high or low. Since reference ranges usually represent two standard deviations above or below the average mean value in healthy people, it is still normal for about five percent of the population to be ever so slightly higher or lower than the reference range. Keeping this in mind, a slightly low or high result is rarely concerning. Tests may also be slightly off due to illness, food or drink consumed, or many other factors. Many doctors will advise repeating the tests at a later time to see if the value has normalized, remains the same, or is trending upward or downward. -
Handout Page 1 of 8
MLT 112: Principles of Phlebotomy Learning Unit 3: Handout Specimen Collection – Venipuncture Procedure I. Collecting and Processing of Specimens A. Blood 1. Venipuncture Procedure (arm + dorsal/hand) – vacutainer, syringe, butterfly a. Approaching the Patient Correct patient identification Wash hands Have patient recite his/her name Wrist identification – mandatory must match requisition check ankle on babies and peds Out-patients – ask patient to spell name In-patients – see wrist ID Unconscious patient – see wrist ID Unidentified patient – emergency – use temporary I.D. band non-emergency – wait for I.D. Explanation and Reassurance – Inspire confidence Conversation Ensure that patient has complied with test requirements, such as fasting (only water), NPO (nothing per oral), etc. Check for any allergies, such as to latex, adhesive bandages, etc. b. Positions Positioning the patient Vein accessibility Sitting vs. lying down Phlebotomist position – always in front in case of fainting. c. Applying the Tourniquet See previous lecture Page 1 of 8 MLT 112: Principles of Phlebotomy Learning Unit 3: Handout d. Veins Used - Antecubital Fossa Cephalic Median Cephalic Median Basilic Median Cubital Vein – vein of choice, anchored best Other Structures – avoid Brachial artery – apply pressure 5 minutes Cutaneous nerve – very painful Tendon for the biceps muscle – always draw below crease e. Other Vein Sites Wrist (never palm side) Hand Ankle Foot f. Preparing Equipment Syringes Assembly – always work plunger before procedure. Plunger position – must -
Human Physiology an Integrated Approach
Gas Exchange and Transport Gas Exchange in the Lungs and Tissues 18 Lower Alveolar P Decreases Oxygen Uptake O2 Diff usion Problems Cause Hypoxia Gas Solubility Aff ects Diff usion Gas Transport in the Blood Hemoglobin Binds to Oxygen Oxygen Binding Obeys the Law of Mass Action Hemoglobin Transports Most Oxygen to the Tissues P Determines Oxygen-Hb Binding O2 Oxygen Binding Is Expressed As a Percentage Several Factors Aff ect Oxygen-Hb Binding Carbon Dioxide Is Transported in Three Ways Regulation of Ventilation Neurons in the Medulla Control Breathing Carbon Dioxide, Oxygen, and pH Infl uence Ventilation Protective Refl exes Guard the Lungs Higher Brain Centers Aff ect Patterns of Ventilation The successful ascent of Everest without supplementary oxygen is one of the great sagas of the 20th century. — John B. West, Climbing with O’s , NOVA Online (www.pbs.org) Background Basics Exchange epithelia pH and buff ers Law of mass action Cerebrospinal fl uid Simple diff usion Autonomic and somatic motor neurons Structure of the brain stem Red blood cells and Giant liposomes hemoglobin of pulmonary Blood-brain barrier surfactant (40X) From Chapter 18 of Human Physiology: An Integrated Approach, Sixth Edition. Dee Unglaub Silverthorn. Copyright © 2013 by Pearson Education, Inc. All rights reserved. 633 Gas Exchange and Transport he book Into Thin Air by Jon Krakauer chronicles an ill- RUNNING PROBLEM fated trek to the top of Mt. Everest. To reach the summit of Mt. Everest, climbers must pass through the “death zone” T High Altitude located at about 8000 meters (over 26,000 ft ). Of the thousands of people who have attempted the summit, only about 2000 have been In 1981 a group of 20 physiologists, physicians, and successful, and more than 185 have died. -
Vacutainer Tubes
Vacutainer Tube Guide - Order of Draw for routine volumes See separate guide for micro-tainers Number of Tube Inversions at Label Blood Collection Volume Tubes/Bottles Abbrev. Additive (Do Not Shake) General Laboratory Use Draw 1 Aerobic and 1 5 Blood Culture / requires Adapter 20 mL Anaerobic Culture Note: A separate venipuncture for trace BLCLT Bottles metal analysis is required if blood cultures are ordered at the same time. NAYYP Clot activator 8 Trace Element serum tube for Copper 6 mL Royal Trace Element whole blood for Aluminum, NAVYE 2 Call 2-5002 Blue K EDTA Lead, Zinc, Selenium, Manganese, Mercury, 8 for tubes Cadmium, Arsenic Discard tube or secondary sterile specimen 6 mL Clear None 0 tube 3 mL 2.7 mL Light (Blue) BLUE Sodium Citrate (3.2%) Coagulation Testing on Plasma Blue 3-4 1.8 mL (Blue/clear) Serum determinations where a gel-barrier is 6 mL Red RED Clot Activator 5 contraindicated; Therapeutic Drug 4 mL Monitoring (TDM) Clot Activator and gel SST - Serum determinations in Chemistry, Gold SST 5 5 mL for serum separation Immunology, Serology; HLA Ab screen, DSA Light Lithium heparin and PST PST - Plasma determinations in Chemistry 4.5 mL Green gel - plasma separation 8 GLI Lithium heparin 8 Whole Blood Plasma: Troponin, Lactic Acid, Ion. Ca+ 6 mL Dark 4 mL Green GNA Sodium heparin 8 Whole Blood Plasma: Tissue typing, plasma hemoglobin, plasma catecholamine, chromosome analysis Pink PINK Dry K2EDTA 8 Blood Bank / Transfusion; HLA Typing 6 mL Tan TAN K2EDTA 8 Lead Testing only 3 mL LAV 4 Whole Blood Hematology; some Flow, 4 mL Lavender Dry K2EDTA 8 Molecular, and Genetic tests; HIV Viral 2 mL LAV6 Load Whole Blood tube with Glycolytic inhibitor Potassium Oxalate / for GTT, Gray 6 mL GRAY Sodium fluoride 8 Post-Prandial Glucose, D-Xylose, and Volatiles panel ACDA ACD Whole Blood tube for Flow Yellow (Acid Citrate Dextrose 8 immunophenotyping; HLA cross matching; 8.5 mL ACDB Solution A) genetic and other referred tests ALWAYS Check Expiration Dates before use! If unsure of use, refer to the on-line “Lab User’s Guide” or call the lab at 25002 . -
Section 8: Hematology CHAPTER 47: ANEMIA
Section 8: Hematology CHAPTER 47: ANEMIA Q.1. A 56-year-old man presents with symptoms of severe dyspnea on exertion and fatigue. His laboratory values are as follows: Hemoglobin 6.0 g/dL (normal: 12–15 g/dL) Hematocrit 18% (normal: 36%–46%) RBC count 2 million/L (normal: 4–5.2 million/L) Reticulocyte count 3% (normal: 0.5%–1.5%) Which of the following caused this man’s anemia? A. Decreased red cell production B. Increased red cell destruction C. Acute blood loss (hemorrhage) D. There is insufficient information to make a determination Answer: A. This man presents with anemia and an elevated reticulocyte count which seems to suggest a hemolytic process. His reticulocyte count, however, has not been corrected for the degree of anemia he displays. This can be done by calculating his corrected reticulocyte count ([3% × (18%/45%)] = 1.2%), which is less than 2 and thus suggestive of a hypoproliferative process (decreased red cell production). Q.2. A 25-year-old man with pancytopenia undergoes bone marrow aspiration and biopsy, which reveals profound hypocellularity and virtual absence of hematopoietic cells. Cytogenetic analysis of the bone marrow does not reveal any abnormalities. Despite red blood cell and platelet transfusions, his pancytopenia worsens. Histocompatibility testing of his only sister fails to reveal a match. What would be the most appropriate course of therapy? A. Antithymocyte globulin, cyclosporine, and prednisone B. Prednisone alone C. Supportive therapy with chronic blood and platelet transfusions only D. Methotrexate and prednisone E. Bone marrow transplant Answer: A. Although supportive care with transfusions is necessary for treating this patient with aplastic anemia, most cases are not self-limited. -
Bone Marrow (Stem Cell) Transplant for Sickle Cell Disease Bone Marrow (Stem Cell) Transplant
Bone Marrow (Stem Cell) Transplant for Sickle Cell Disease Bone Marrow (Stem Cell) Transplant for Sickle Cell Disease 1 Produced by St. Jude Children’s Research Hospital Departments of Hematology, Patient Education, and Biomedical Communications. Funds were provided by St. Jude Children’s Research Hospital, ALSAC, and a grant from the Plough Foundation. This document is not intended to take the place of the care and attention of your personal physician. Our goal is to promote active participation in your care and treatment by providing information and education. Questions about individual health concerns or specifi c treatment options should be discussed with your physician. For more general information on sickle cell disease, please visit our Web site at www.stjude.org/sicklecell. Copyright © 2009 St. Jude Children’s Research Hospital How did bone marrow (stem cell) transplants begin for children with sickle cell disease? Bone marrow (stem cell) transplants have been used for the treatment and cure of a variety of cancers, immune system diseases, and blood diseases for many years. Doctors in the United States and other countries have developed studies to treat children who have severe sickle cell disease with bone marrow (stem cell) transplants. How does a bone marrow (stem cell) transplant work? 2 In a person with sickle cell disease, the bone marrow produces red blood cells that contain hemoglobin S. This leads to the complications of sickle cell disease. • To prepare for a bone marrow (stem cell) transplant, strong medicines, called chemotherapy, are used to weaken or destroy the patient’s own bone marrow, stem cells, and infection fi ghting system. -
A Rapid and Quantitative D-Dimer Assay in Whole Blood and Plasma on the Point-Of-Care PATHFAST Analyzer ARTICLE in PRESS
MODEL 6 TR-03106; No of Pages 7 ARTICLE IN PRESS Thrombosis Research (2007) xx, xxx–xxx intl.elsevierhealth.com/journals/thre REGULAR ARTICLE A rapid and quantitative D-Dimer assay in whole blood and plasma on the point-of-care PATHFAST analyzer Teruko Fukuda a,⁎, Hidetoshi Kasai b, Takeo Kusano b, Chisato Shimazu b, Kazuo Kawasugi c, Yukihisa Miyazawa b a Department of Clinical Laboratory Medicine, Teikyo University School of Medical Technology, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, Japan b Department of Central Clinical Laboratory, Teikyo University Hospital, Japan c Department of Internal Medicine, Teikyo University School of Medicine, Japan Received 11 July 2006; received in revised form 7 December 2006; accepted 28 December 2006 KEYWORDS Abstract The objective of this study was to evaluate the accuracy indices of the new D-Dimer; rapid and quantitative PATHFAST D-Dimer assay in patients with clinically suspected Deep-vein thrombosis; deep-vein thrombosis (DVT). Eighty two consecutive patients (34% DVT, 66% non-DVT) Point-of-care testing; with suspected DVT of a lower limb were tested with the D-Dimer assay with a Chemiluminescent PATHFAST analyzer. The diagnostic value of the PATHFAST D-Dimer assay (which is immunoassay based on the principle of a chemiluminescent enzyme immunoassay) for DVT was evaluated with pre-test clinical probability, compression ultrasonography (CUS). Furthermore, each patient underwent contrast venography and computed tomogra- phy, if necessary. The sensitivity and specificity of the D-Dimer assay using 0.570 μg/ mL FEU as a clinical cut-off value was found to be 100% and 63.2%, respectively, for the diagnosis of DVT, with a positive predictive value (PPV) and negative predictive value (NPV) of 66.7% and 100%, respectively. -
The Complete Blood Count Sample Report 1
THE COMPLETE BLOOD COUNT SAMPLE REPORT 1. Name and address of the lab where the test was performed. Tests may be run in a physician office lab, a lab located in a Different laboratories generate reports that can vary greatly in appearance and in the order and kind of The Complete Blood Count Sample Report clinic or hospital, and/or samples may be sent to a reference information included. This is one example of what a lab report for a Complete Blood Count may look like. laboratory for analysis. NamesDifferent and laboratories places used generate have been reports made that up can for vary illustrative greatly inpurposes appearance only. andThe innumbered the order keyand tokind the of right 2. Date this copy of the report was printed. This date may be explainsinformation a few included. of the reportThis is elements.one exampl e of what a lab report for a Complete Blood Count may look like. different than the date the results were generated, especially Names and places used have been made up for illustrative purposes only. Point your cursor at a number on cumulative reports (those that include results of several to learn about the different report elements. different tests run on different days). 3. Patient name or identifier. Links results to the correct person. 1 University Medical Center, Dept. of Pathology Report Date/Time: 123 University Way, City, ST 12345 02/10/2014 16:40 2 4. Patient identifier and identification number. Links results to the correct person. 3 Name: Doe, John Q. Age/Sex: 73/M DOB: 01/01/1941 5. -
Myelodysplastic Syndromes Overview and Types
cancer.org | 1.800.227.2345 About Myelodysplastic Syndromes Overview and Types If you have been diagnosed with a myelodysplastic syndrome or are worried about it, you likely have a lot of questions. Learning some basics is a good place to start. ● What Are Myelodysplastic Syndromes? ● Types of Myelodysplastic Syndromes Research and Statistics See the latest estimates for new cases of myelodysplastic syndromes in the US and what research is currently being done. ● Key Statistics for Myelodysplastic Syndromes ● What's New in Myelodysplastic Syndrome Research? What Are Myelodysplastic Syndromes? Myelodysplastic syndromes (MDS) are conditions that can occur when the blood- forming cells in the bone marrow become abnormal. This leads to low numbers of one or more types of blood cells. MDS is considered a type of cancer1. Normal bone marrow 1 ____________________________________________________________________________________American Cancer Society cancer.org | 1.800.227.2345 Bone marrow is found in the middle of certain bones. It is made up of blood-forming cells, fat cells, and supporting tissues. A small fraction of the blood-forming cells are blood stem cells. Stem cells are needed to make new blood cells. There are 3 main types of blood cells: red blood cells, white blood cells, and platelets. Red blood cells pick up oxygen in the lungs and carry it to the rest of the body. These cells also bring carbon dioxide back to the lungs. Having too few red blood cells is called anemia. It can make a person feel tired and weak and look pale. Severe anemia can cause shortness of breath. White blood cells (also known as leukocytes) are important in defending the body against infection. -
Understanding Your Blood Test Lab Results
Understanding Your Blood Test Lab Results A comprehensive "Health Panel" has been designed specifically to screen for general abnormalities in the blood. This panel includes: General Chemistry Screen or (SMAC), Complete Blood Count or (CBC), and Lipid examination. A 12 hour fast from all food and drink (water is allowed) is required to facilitate accurate results for some of the tests in this panel. Below, is a breakdown of all the components and a brief explanation of each test. Abnormal results do not necessarily indicate the presence of disease. However, it is very important that these results are interpreted by your doctor so that he/she can accurately interpret the findings in conjunction with your medical history and order any follow-up testing if needed. The Bernards Township Health Department and the testing laboratory cannot interpret these results for you. You must speak to your doctor! 262 South Finley Avenue Basking Ridge, NJ 07920 www.bernardshealth.org Phone: 908-204-2520 Fax: 908-204-3075 1 Chemistry Screen Components Albumin: A major protein of the blood, albumin plays an important role in maintaining the osmotic pressure spleen or water in the blood vessels. It is made in the liver and is an indicator of liver disease and nutritional status. A/G Ratio: A calculated ratio of the levels of Albumin and Globulin, 2 serum proteins. Low A/G ratios can be associated with certain liver diseases, kidney disease, myeloma and other disorders. ALT: Also know as SGPT, ALT is an enzyme produced by the liver and is useful in detecting liver disorders. -
Evaluation of Complete Blood Count Parameters for Diagnosis In
Original Investigation / Özgün Araştırma DOI: 10.5578/ced.68886 • J Pediatr Inf 2020;14(2):e55-e62 Evaluation of Complete Blood Count Parameters for Diagnosis in Children with Sepsis in the Pediatric Intensive Care Unit Pediatrik Yoğun Bakım Ünitesinde Sepsisli Çocuklarda Tanı İçin Tam Kan Sayımı Parametrelerinin Değerlendirilmesi Fatih Aygün1(İD), Cansu Durak1(İD), Fatih Varol1(İD), Haluk Çokuğraş2(İD), Yıldız Camcıoğlu2(İD), Halit Çam1(İD) 1 Department of Pediatric Intensive Care, Istanbul University School of Cerrahpasa Medicine, Istanbul, Turkey 2 Department of Pediatric Infectious Diseases, Istanbul University School of Cerrahpasa Medicine, Istanbul, Turkey Cite this article as: Aygün F, Durak C, Varol F, Çokuğraş H, Camcıoğlu Y, Çam H. Evaluation of complete blood count parameters for diagnosis in children with sepsis in the pediatric intensive care unit. J Pediatr Inf 2020;14(2):e55-e62. Abstract Öz Objective: Early diagnosis of sepsis is important for effective treatment Giriş: Erken tanı sepsiste etkili tedavi ve iyi prognoz için önemlidir. C-re- and improved prognosis. C-reactive protein (CRP) and procalcitonin aktif protein (CRP) ve prokalsitonin (PKT) sepsiste en sık kullanılan bi- (PCT) are the most commonly used biomarkers for sepsis. However, their yobelirteçlerdir. Fakat rutinde kullanımı maliyet etkin değildir. Tam kan routine usage is not cost-effective. Complete Blood Count (CBC) param- sayımı (TKS) parametrelerinden eritrosit dağılım genişliği (EDG), nötrofil eters including red cell distribution width (RDW), neutrophil to lympho- lenfosit oranı (NLO), trombosit lenfosit oranı (TLO), ortalama trombosit cyte ratios (NLR), platelet to lymphocyte ratios (PLR), mean platelet vol- hacmi (OTH) basit ve kolay olarak hesaplanmaktadır. Bu çalışmanın ama- ume (MPV), and hemoglobin are simple and easily calculated.