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Dinoflagellate Nucleus Contains an Extensive Endomembrane Network, the Nuclear Net

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Dinoflagellate Nucleus Contains an Extensive Endomembrane Network, the Nuclear Net Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net Author Gregory S. Gavelis, Maria Herranz, Kevin C. Wakeman, Christina Ripken, Satoshi Mitarai, Gillian H. Gile, Patrick J. Keeling, Brian S. Leander journal or Scientific Reports publication title volume 9 number 1 page range 839 year 2019-01-29 Publisher Nature Research Rights (C) 2019 The Author(s). Author's flag publisher URL http://id.nii.ac.jp/1394/00000889/ doi: info:doi/10.1038/s41598-018-37065-w Creative Commons? Attribution 4.0 International? (https://creativecommons.org/licenses/by/4.0/) www.nature.com/scientificreports OPEN Dinofagellate nucleus contains an extensive endomembrane network, the nuclear net Received: 20 April 2018 Gregory S. Gavelis1,2,3, Maria Herranz2,3, Kevin C. Wakeman4, Christina Ripken5, Accepted: 28 November 2018 Satoshi Mitarai6, Gillian H. Gile 1, Patrick J. Keeling2 & Brian S. Leander1,2 Published: xx xx xxxx Dinofagellates are some of the most common eukaryotic cells in the ocean, but have very unusual nuclei. Many exhibit a form of closed mitosis (dinomitosis) wherein the nuclear envelope (NE) invaginates to form one or more trans-nuclear tunnels. Rather than contact spindles directly, the chromatids then bind to membrane-based kinetochores on the NE. To better understand these unique mitotic features, we reconstructed the nuclear architecture of Polykrikos kofoidii in 3D using focused ion beam scanning electron microscopy (FIB-SEM) in conjunction with high-pressure freezing, freeze- substitution, TEM, and confocal microscopy. We found that P. kofoidii possessed six nuclear tunnels, which were continuous with a reticulating network of membranes that has thus far gone unnoticed. These membranous extensions interconnect the six tunnels while ramifying throughout the nucleus to form a “nuclear net.” To our knowledge, the nuclear net is the most elaborate endomembrane structure described within a nucleus. Our fndings demonstrate the utility of tomographic approaches for detecting 3D membrane networks and show that nuclear complexity has been underestimated in Polykrikos kofoidii and, potentially, in other dinofagellates. Dinofagellate nuclei (dinokarya) have long fascinated cell biologists because of their bizarre features. Tey con- tain some of the largest eukaryotic genomes, housed in dozens to hundreds of chromosomes that remain per- manently condensed throughout the cell cycle1,2. Te chromosomes are characteristically dense, some existing in a “liquid crystalline state,” while all seem to lack nucleosomes3–5. Phylogenomic reconstructions,6,7 and recent experimental work8, suggest that nucleosomes were lost in the common ancestor of all dinofagellates and that their DNA packing role was taken over by nucleoproteins acquired from a virus. Dinofagellate genome archi- tecture is highly unusual, with genes arranged unidirectionally, ofen as tandem repeats, and the vast majority of genomic DNA is noncoding9–11. Te sparse coding regions probably occupy “loops” of DNA at the chromo- some periphery, which are organized by histone-like proteins of bacterial origin12–14. In the past decade, new approaches have illuminated the unusual arrangement of proteins and DNA within dinofagellate chromosomes, as well as their coordination throughout the cell cycle15–17. However, much less attention has been paid to the membranes that surround them (i.e., the nuclear envelope). Te nuclear envelope (NE) and the endoplasmic reticulum—which are continuous—together constitute the most conserved organelle(s) in eukaryotic history, given that even mitochondria, the Golgi apparatus, and fa- gella have been abandoned in certain eukaryotic lineages18–20. Besides acting as a gatekeeper to the nucleus, the dinofagellate NE takes on an unusual conformation during mitosis, called “dinomitosis” in core dinofagellates (i.e., dinofagellates other than Oxyrrhis and syndinians). By defnition, dinomitosis is a form of closed mitosis, since the NE never breaks down. Instead, it pinches inward at each nuclear pole to form a “tunnel” through the nucleus; essentially turning the nucleus into a toroidal shape resembling a doughnut. By traversing the tunnel, cytoplasmic spindles are able to cross the dinokaryon without ever entering the nucleoplasm. Tis stands in con- trast to most organisms with closed mitosis, which use either intra-nuclear or NE-spanning spindles to separate 1School of Life Sciences, Arizona State University, Tempe, AZ, 85287-4501, USA. 2Department of Botany, University of British Columbia, Vancouver, BC, V6T-1Z4, Canada. 3Department of Zoology, University of British Columbia, Vancouver, BC, V6T-1Z4, Canada. 4Department of Biological Sciences: Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan. 5Okinawa Institute of Science and Technology, Graduate University, Kunigami-gun, Okinawa, 904-0495, Japan. 6Marine Biophysics Unit, Okinawa Institute of Science and Technology, Kunigami- gun, Okinawa, 904-0495, Japan. Correspondence and requests for materials should be addressed to G.S.G. (email: [email protected]) SCIENTIFIC REPORTS | (2019) 9:839 | https://doi.org/10.1038/s41598-018-37065-w 1 www.nature.com/scientificreports/ Figure 1. Cellular features of Polykrikos kofoidii. (A) Diferential interference contrast (DIC) light micrograph of a P. kofoidii pseudocolony, which is defned by the presence of two nuclei (Nu) and nematocysts (N). (B) Maximum intensity projection of several FIB-SEM sections showing a nematocyst (N), a taeniocyst (T) and the side of a nucleus (Nu). (C) FIB-SEM surface reconstruction of several chromosomes. Scale bar A = 30 µm, B = 10 µm, C = 2 µm. the chromatids21–23. Uniquely, dinomitotic chromatids never directly contact the spindles; instead they attach to membrane-bound kinetochores on the inner NE membrane. Te chromatids then migrate to opposite ends of the membranous tunnel24. Once segregation is complete, the nucleus divides and the tunnel pinches apart in the mid- dle, returning each daughter nucleus to a spherical shape25–27. Where studied, early-branching dinomitotic lineages have a single tunnel (e.g., Noctiluca scintillans, Syndinium sp., and Haplozoon—if it is early branching), while core dinofagellates diverging afer N. scintillans (e.g. Amphidinium carterae, Prorocentrum minium, Heterocapsa sp., and Crypthecodinium cohnii) have multiple tunnels in parallel—with a maximum of five, as described in C. cohnii28. Tus, like the evolution of dinofagellate chromosomes, mitosis has become increasingly unusual and elaborate in some dinofagellates29. Te details of dinomitosis deserve deeper investigation, given that dinofag- ellate cell cycles proceed at a curiously slow pace compared to other algae30, which has important ecological con- sequences in the marine plankton, where dinofagellates are among the most common algae and consumers31,32. Here, we investigated the complexity of nuclear membranes in Polykrikos kofoidii, a large, predatory dinofag- ellate. While most dinomitotic studies have focused on cells with modestly sized nuclei33–36 (e.g., Crypthecodinium cohnii, in which nuclei are ~10 µm wide and contain 99 to 110 chromosomes), P. kofoidii is an interesting subject because its nuclei are giant—at ~40 µm in diameter—and each contains hundreds of chromosomes. Moreover, P. kofoidii is “pseudocolonial” with eight fagella and two nuclei per cell (Fig. 1A), compared to the typical comple- ment of two fagella and one nucleus per dinofagellate cell37. Polykrikoids are ecologically important as voracious predators of harmful algal blooms, which they capture using elaborate secretory organelles (Fig. 1B)38–40, and can consume multiple cells of chain-forming prey at a time, in part facilitated by the large size of their pseudocol- onies41,42. Te nuclei in P. kofoidii are correspondingly giant, and each is tethered to the nearest pair of fagellar basal bodies by fbrous ribbons. Previous studies have shown its NE to possess bubble-like convexities (“nuclear chambers”) and multiple tunnels during mitosis43. We investigated dinomitotic membrane architecture in greater depth, by using focused ion beam scanning electron microscopy (FIB-SEM) to digitally reconstruct a giant nucleus from P. kofoidii in 3D, and confrmed our fndings using immuno-fuorescence confocal microscopy and TEM on a dozen additional specimens. Prior to this study, only one dinofagellate nucleus had been modeled in 3D; a mitotic dinokaryon in C. cohnii, which was inferred from serial section transmission electron microscopy (TEM) on a single specimen prepared using stand- ard chemical fxation28. Our study is the frst attempt to reconstruct a dinokaryon from FIB-SEM data, which we used in combination with improved fxation techniques—high-pressure freezing and freeze-substitution—specif- ically chosen to minimize membrane artifacts. In addition to confrming known features of dinofagellate nuclei (e.g., NE tunnels), we also uncovered a novel membranous network, which ramifed throughout the nucleus into a sprawling “nuclear net” that interlinked the six tunnels. Tis web-like, membranous structure represents a new level of complexity for the dinokaryon. Results We frst conducted a TEM investigation on several chemically-fxed mitotic cells of Polykrikos kofoidii and con- frmed previously noted43 features, such as (1) chromosomes that associate with the NE of dinomitotic tunnels (Fig. 2B,C), as well as (2) elaborate infoldings of the plasma membranes that are each called a “pusule” (Figs 2D,E and 3) the presence of “fbrous ribbons” that tether
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