Botany DNA barcoding of a complex genus, Aesculus L. (Sapindaceae) reveals lack of species-level resolution Journal: Botany Manuscript ID cjb-2019-0018.R2 Manuscript Type: Article Date Submitted by the 10-May-2019 Author: Complete List of Authors: Aygoren Uluer, Deniz; Ahi Evran Universitesi, Cicekdagi Vocational College, Department of Plant and Animal Production; Alshamrani, Rahma; King Abdulaziz University, Department of Biological Sciences Draft Keyword: Aesculus, DNA barcoding, ITS/ITS2, matK, phylogeny Is the invited manuscript for consideration in a Special Not applicable (regular submission) Issue? : https://mc06.manuscriptcentral.com/botany-pubs Page 1 of 28 Botany DNA barcoding of a complex genus, Aesculus L. (Sapindaceae) reveals lack of species-level resolution Deniz Aygoren Uluer1,3, Rahma Alshamrani 2 1 Ahi Evran University, Cicekdagi Vocational College, Department of Plant and Animal Production, Boyalık Mahallesi, Stadyum Caddesi, Turan Sok. No:18 40700 Cicekdagi, Kirşehir, Turkey.
[email protected] 2 King Abdulaziz University, Department of Biological Sciences, PO Box 80206, 21589, Jeddah, Saudi Arabia, email:
[email protected]. Draft 3Author for correspondence: Deniz Aygoren Uluer, Ahi Evran University, Cicekdagi Vocational College, Department of Plant and Animal Production, Boyalık Mahallesi, Stadyum Caddesi, Turan Sok. No:18 40700 Cicekdagi, Kirşehir, Turkey, email:
[email protected], Work phone: +903862805500, Fax: +903862805528. 1 https://mc06.manuscriptcentral.com/botany-pubs Botany Page 2 of 28 Abstract Aesculus L. is a small genus of horticulturally important trees and shrubs, comprising 13-19 species. Frequent hybridization among species, particularly in cultivation, has contributed to taxonomic confusion and difficulties in identification of plants. In this study, we evaluated three widely employed plant DNA barcode loci, matK, and the entire ITS region (ITS1+5.8S+ITS2) as well as subunit ITS2 for 50 individuals representing 13 species of Aesculus, excluding only A.