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Methods in Cardiac Research

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Methods in Cardiac Research Methods in Cardiac Research Monday 20th to Wednesday 22nd April 2020 At Institute for Experimental Medical Research Building 7, 4th floor Oslo University Hospital Ullevål Course administration: Mani Sadredini, Andreas Romaine, Kjersti Blom, Chloe Rixon and Mathis Korseberg Stokke Program and time schedule MONDAY APRIL 20th 2020 10:00 - 16:05................ Lectures and practical demonstrations 16:05 - …..………………….. Networking with pizza TUESDAY APRIL 21st 2020 08:00 - 16:00……………… Lectures and practical demonstrations WEDNESDAY APRIL 22nd 2020 08:00 - 14:10……………… Lectures and practical demonstrations 14:30 - 15:30……………… Course test GENERAL INFORMATION Organizers and contact persons This course is hosted at the Institute for Experimental Medical Research (IEMR) at Ullevål Mani Sadredini hospital. The institute is located on the 4th floor of [email protected] building 7 and signs will be visible from the main 95286734 entrance of the hospital (building 4). If you are delayed or require assistance please contact Mani Andreas Romaine or Andreas. Lunch is covered by NORHEART for all [email protected] three days and pizza is provided at the end of day 1. 45294007 Kjersti Blom [email protected] Chloe Rixon [email protected] Contact person, NORHEART Mathis Korseberg Stokke [email protected] Detailed program MONDAY APRIL 20TH 10.00-10.10 Introductory remarks Mani Sadredini, Andreas Romaine 10.10-10.30 Cardiac translational research – from bench to bedside Ivar Sjaastad PART I: METHODS IN MOLECULAR BIOLOGY 10.30-10.45 Cell cultures in cardiac research Pugazendhi Murugan Erusappan 10.45-10.50 Buzz 10.50-11.10 Coffee break 11.10-11.25 Gene expression in the heart: Quantitative PCR Arne Olav Melleby 11.25-11.30 Buzz 11.30-11.45 RNA sequencing Karoline Bjarnesdatter Rypdal 11.45-11.50 Buzz 11.50-12.15 Student presentations 12.15-12.45 Free lunch at Ullevål Hotell 13.00-13.15 Proteins: Separation, purification and quantification Marianne Lunde 13.15-13.20 Buzz 13.20-13.35 Protein localization: Flow cytometry & ELISA Andreas Romaine 13.35-13.40 Buzz 13.40-13.55 Protein-protein interactions Cathrine Carlson 13.55-14.00 Buzz 14.00-14.20 Coffee break 14.20-14.45 Student presentations 14.45-15.00 Detection of cyclic nucleotides and protein-protein interactions: FRET in cardiac research Kjetil Wessel Andressen 15.00-15.05 Buzz 15.05-16.05 Practical Demonstration 1: Methods in Molecular biology 16.05- Networking with pizza TUESDAY APRIL 21ST PART II: CARDIAC FUNCTION AND PHENOTYPING IN SMALL ANIMALS 08.00-08.15 Exercise training and testing of small animals Mani Sadredini 08.15-08.20 Buzz 08.20-08.35 The isolated perfused heart: Langendorff preparation May-Kristin Torp 08.35-08.40 Buzz 08.40-08.55 Genetically modified mice in heart failure research Arne Olav Melleby 08.55-09.00 Buzz 09.00-09.20 Coffee break 09.20-09.50 Cardiac imaging techniques and hemodynamic measurements for small animals Einar Sjaastad Nordén 09.50-09.55 Buzz 09.55-10.25 Student presentations 10.25-10.45 Coffee break 10.45-12.00 Practical Demonstration 2: Assessment of Cardiac Function in Small Animals 12.15-12.45 Free lunch at Ullevål Hotell PART III: METHODS IN CARDIAC CELLULAR BIOLOGY 13.00-13.30 Imaging techniques for assessment of cardiomyocyte structure and function Xin Shen, Yufeng Hou 13:30-13.35 Buzz 13.35-14.00 Student presentations 14.00-14.15 Assessment of cellular electrophysiology: Patch-clamp technique Martin Laasmaa 14.15-14.20 Buzz 14.20-14.40 Coffee break 14.40-14.55 Investigating effects of mechanical stretch on isolated cardiomyocytes Jia Li 14.55-15.00 Buzz 15.00-16.00 Practical Demonstration 3: Methods in Cardiac Cellular Biology WEDNESDAY APRIL 22ND PART IV: IMAGING TECHNIQUES IN CARDIAC CLINICAL RESEARCH 08.00-08.30 MRI and CT, SPECT and PET in evaluation of cardiac function Einar Hopp, Anders Hodt, Anne Günther 08.30-08.35 Buzz 08.35-09.00 Student presentations 09.00-10.00 Interactive practical session on imaging 10.00-10.20 Coffee break 10.20-10.35 Large animal models of heart disease TBA 10.35-10.40 Buzz 10.40-11.00 Student presentations 11.00-12.00 Practical Demonstration: large animal models in cardiac research 12.15-12.45 Free lunch at Ullevål Hotell 13.00-13.30 Cardiac translational research Ingebjørg Seljeflot, Maria Vistnes 13.30-13.35 Buzz 13.35-14.20 Student presentations 14.20-14.40 Coffee break 14.40-15.30 COURSE TEST 15.30 Course end LECTURERS Andressen, Kjetil Wessel Research Scientist, Dep of Pharmacology, UoO Carlson, Cathrine Research Scientist, IEMR, OUH/UoO Eriksen, Morten Head of section, IEMR, UoO/OUH Erusappan, Pugazendhi Murugan Research Associate, IEMR, OUH/UoO Espe, Emil Knut Stenersen Postdoctoral fellow, IEMR, OUH/UoO Frisk, Michael Postdoctoral fellow, IEMR, OUH/UoO Günther, Anne Snr. consultant, Dep of Radiology and Nuclear Med, OUH Hasic, Almira Senior research associate, IEMR, OUH/UoO Hodt, Anders Snr. consultant, Dep of Radiology and Nuclear Med, OUH Hopp, Einar Head of dep., Dep of Radiology and Nuclear Med, OUH Hou, Yufeng Pos tdoctoral fellow, IEMR, OUH/UoO Laasmaa, Martin Postdoctoral fellow, IEMR, OUH/UoO Li, Jia Pos tdoctoral fellow, IEMR, OUH/UoO Lunde, Marianne Research associate, IEMR, OUH/UoO Melleby, Arne Olav PhD student, IEMR, OUH/UoO Nordén, Einar Sjaastad PhD student, IEMR, OUH/UoO Romaine, Andreas PhD student, IEMR, OUH/UoO Rypdal, Karoline Bjarnesdatter PhD student, IEMR, OUH/UoO Sadredini, Mani PhD student, IEMR, OUH/UoO Seljeflot, Ingebjørg Professor, Center for Clinical Heart Res., OUH Shen, Xin Pos tdoctoral fellow, IEMR, OUH/UoO Sjaastad, Ivar Professor, IEMR, UoO/OUH Torp, May-Kristin Postdoctoral fellow, IMB, UoO Vistnes, Maria Postdoctoral fellow, IEMR, OUH/UoO IEMR = Institute for Experimental Medical Research IMB = Institute of Medical Basic Sciences OUH = Oslo University Hospital UoO = University of Oslo Directions to Ullevål hospital The number 20 and 28 bus stop directly outside Ullevål hospital (the stop is called Ullevål sykehus). The hospital is also located next to a light rail station on the Ullevål Hageby Line of the Oslo Tramway. It is located on the south- western side of Ullevål University Hospital, at the intersection of Sognsveien and Kirkeveien. Tickets can be purchased via the Ruter app, at ticket booths or in Narvesen shops. On arriving at the hospital, follow the signs from the main entrance (building 4) to building 7 and climb the stairs to the 4th floor. Map of Ullevål hospital Visiting Oslo Oslo is home to many popular tourist attractions to be enjoyed in the Spring season. Sculpture park in the Frogner Park with more than 200 sculptures by Gustav Vigeland (1869–1943) in bronze, granite and cast iron, including The Angry Boy (Sinnataggen in Norwegian) Oslo's Opera House is located right at the harbour, with an angled, white exterior that appears to rise from the water. It invites its visitors to climb its roof and enjoy panoramic views of Oslo and the fjord, all year round. About NORHEART NORHEART – Norwegian PhD School of Heart Research NORHEART is a national education network for collaborative education of PhD students within cardiovascular research in Norway financed by The Research Council of Norway. NORHEART is initiated, organized and run by cardiovascular scientists. NORHEART is organized as a collaborative network with local nuclei in all university regions: Oslo, Tomsø, Trondheim, Bergen NORHEART events are continuously evolving to promote ongoing and future projects. Therefore all events are based on suggestions and initiatives from active scientists. Some of our upcoming events: Course Metabolic and Mechanical Function of the Heart 2020 University of Tromsø, Date TBA This 3 day course for PhD students emphasizes practical and theoretical knowledge in cardiac function and energy consumption. Workshop Female Heart workshop 2020 Solstrand Hotel & Bad, Bergen, May 19-20 2020 This workshop focuses gender differences in heart disease. TBA Monday April 20th 15.05-16.05 Practical Demonstration 1: Methods in Molecular biology Location Demonstrator 15.10-15.25 15.30-15.45 15.50-16.05 Cell work IEMF Pugazendhi 4th floor Murugan Erusappan PCR IEMF Almira Hasic 4th floor Western IEMF Marianne Lunde 4th floor Tuesday April 21st 10.45-12.00 Practical Demonstration 2: Assessment of Cardiac Function in Small Animals Location Demonstrator 11.00-11.15 11.20-11.35 11.40-11.55 Mouse surgery Build. 25 Arne Olav Melleby Echocardiography Build. 25 Einar Sjaastad on rats Nordén MR for small animal Build. 25 Emil Knut research Stenersen Espe Tuesday April 21st 15.00-16.00 Practical Demonstration 3: Methods in Cardiac Cellular Biology Location Demonstrator 15.00-15.30 15.30-16.00 Microscopy and IEMF 4th Michael Frisk, Group 1 Group 2 patch clamping floor Martin Laasmaa, Xin Shen, Jia Li Isolation of IEMF 5th Yufeng Hou Group 2 Group 1 cardiomyocytes floor Wednesday April 22nd 11.00-12.00 Practical Demonstration 4: Large Animal Surgery in Cardiac Research Location Demonstrator 11.00–12.00 Pigs as experimental IEMF Morten Eriksen All groups models in cardiac 5th floor research .
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