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Methods in Cardiac Research

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Methods in Cardiac Research Methods in Cardiac Research Monday 8th to Wednesday 10th April 2019 At Institute for Experimental Medical Research Building 7, 4th floor Oslo University Hospital Ullevål Course administration: Mani Sadredini, Andreas Romaine and Mathis Korseberg Stokke Program and time schedule MONDAY APRIL 8th 2019 10:00 - 16:05................ Lectures and practical demonstrations 16:05 - …..………………….. Networking with pizza TUESDAY APRIL 9th 2019 08:00 - 16:00……………… Lectures and practical demonstrations WEDNESDAY APRIL 10th 2019 08:00 - 14:10……………… Lectures and practical demonstrations 14:30 - 15:30……………… Course test This 3 day course for PhD students emphasizes GENERAL INFORMATION practical and theoretical knowledge of key methodologies employed in cardiac research. This course is hosted at the Institute for Topics covered include: Experimental Medical Research (IEMR) at Ullevål th Commonly used and cutting edge methods hospital. The institute is located on the 4 floor of in the field of cardiac research building 7 and signs will be visible from the main Methods in molecular biology including: entrance of the hospital (building 4). If you are real-time qPCR, immunolocalisation, delayed or require assistance please contact Mani microarray, western blotting, flow or Andreas (see below). Lunch is covered by cytometry, FRET and ELISA. NORHEART for all three days and pizza is provided Methods in cellular biology including: cell at the end of day 1. isolation and cell culturing, transfection, patch-clamp, cell-stretching, calcium imaging, as well as quantification of Organizers and contact persons, NORHEART receptors and intracellular signaling pathways. Mani Sadredini In vivo and ex vivo testing of cardiac [email protected] function in research animals, such as 95286734 echocardiography, magnetic resonance imaging, pressure-volume measurements, exercise training and cardiac surgery in Andreas Romaine laboratory animals. [email protected] Cardiac examination of patients using MRI, 45294007 CT, SPECT. Mathis Korseberg Stokke This course will give you the skills to: [email protected] Select the appropriate method to use in your research Gain insight into the benefits and limitations of each method Explain the core principles of the presented methods Detailed program MONDAY APRIL 8TH 10.00-10.10 Introductory remarks Mani Sadredini, Andreas Romaine 10.10-10.30 Cardiac translational research – from bench to bedside Ivar Sjaastad PART I: METHODS IN MOLECULAR BIOLOGY 10.30-10.45 Cell cultures in cardiac research Mari Elen Strand 10.45-10.50 Buzz 10.50-11.10 Coffee break 11.10-11.25 Gene expression in the heart: Quantitative PCR Arne Olav Melleby 11.25-11.30 Buzz 11.30-11.45 RNA sequencing Karoline Bjarnesdatter Rypdal 11.45-11.50 Buzz 11.50-12.15 Student presentations 12.15-12.45 Free lunch at Ullevål Hotell 13.00-13.15 Proteins: Separation, purification and quantification Marianne Lunde 13.15-13.20 Buzz 13.20-13.35 Protein localization: Flow cytometry & ELISA Andreas Romaine 13.35-13.40 Buzz 13.40-13.55 Protein-protein interactions Cathrine Carlson 13.55-14.00 Buzz 14.00-14.20 Coffee break 14.20-14.45 Student presentations 14.45-15.00 Detection of cyclic nucleotides and protein-protein interactions: FRET in cardiac research Kjetil Wessel Andressen 15.00-15.05 Buzz 15.05-16.05 Practical Demonstration 1: Methods in Molecular biology 16.05- Networking with pizza TUESDAY APRIL 9TH PART II: CARDIAC FUNCTION AND PHENOTYPING IN SMALL ANIMALS 08.00-08.15 Exercise training and testing of small animals Mani Sadredini 08.15-08.20 Buzz 08.20-08.35 The isolated perfused heart: Langendorff preparation Kåre-Olav Stensløkken 08.35-08.40 Buzz 08.40-08.55 Genetically modified mice in heart failure research Arne Olav Melleby 08.55-09.00 Buzz 09.00-09.20 Coffee break 09.20-09.50 Cardiac imaging techniques and hemodynamic measurements for small animals Einar Sjaastad Nordén 09.50-09.55 Buzz 09.55-10.25 Student presentations 10.25-10.45 Coffee break 10.45-12.00 Practical Demonstration 2: Assessment of Cardiac Function in Small Animals 12.15-12.45 Free lunch at Ullevål Hotell PART III: METHODS IN CARDIAC CELLULAR BIOLOGY 13.00-13.30 Imaging techniques for assessment of cardiomyocyte structure and function Terje Kolstad 13:30-13.35 Buzz 13.35-14.00 Student presentations 14.00-14.15 Assessment of cellular electrophysiology: Patch-clamp technique Jonas Skogestad 14.15-14.20 Buzz 14.20-14.40 Coffee break 14.40-14.55 Investigating effects of mechanical stretch on isolated cardiomyocytes and cardiac muscle tissue Marianne Ruud, Jia Li 14.55-15.00 Buzz 15.00-16.00 Practical Demonstration 3: Methods in Cardiac Cellular Biology WEDNESDAY APRIL 10TH PART IV: IMAGING TECHNIQUES IN CARDIAC CLINICAL RESEARCH 08.00-08.30 MRI and CT, SPECT and PET in evaluation of cardiac function Einar Hopp, Anders Hodt, Anne Günther 08.30-08.35 Buzz 08.35-09.00 Student presentations 09.00-10.00 Interactive practical session on imaging 10.00-10.20 Coffee break 10.20-10.35 Large animals in cardiovascular research Ole-Jakob How 10.35-10.40 Buzz 10.40-11.00 Student presentations 11.00-12.00 Practical Demonstration 4: Large Animal Surgery in Cardiac Research 12.15-12.45 Free lunch at Ullevål Hotell 13.00-13.20 Cardiac translational research – from bedside to bench Ingebjørg Seljeflot 13.25-14.10 Student presentations 14.10-14.30 Coffee break 14.30-15.30 COURSE TEST 15.30 Course end LECTURERS Andressen, Kjetil Wessel Research Scientist, Dep of Pharmacology, UoO Carlson, Cathrine Research Scientist, IEMR, OUH/UoO Eriksen, Morten Head of section, IEMR, UoO/OUH Frisk, Michael Pos tdoctoral fellow, IEMR, OUH/UoO Günther, Anne Snr. consultant, Dep of Radiology and Nuclear Med, OUH Hasic, Almira Senior research associate, IEMR, OUH/UoO Henriksen, Ida Marie Research associate, IEMR, OUH/UoO Hopp, Einar Snr. consultant, Dep of Radiology and Nuclear Med, OUH Hodt, Anders Snr. consultant, Dep of Radiology and Nuclear Med, OUH Hou, Yufeng Pos tdoctoral fellow, IEMR, OUH/UoO How, Ole-Jakob Professor, Institute for Medical Biology, University of Tromsø Kolstad, Terje PhD student, IEMR, OUH/UoO Li, Jia Pos tdoctoral fellow, IEMR, OUH/UoO Lunde, Marianne Research associate, IEMR, OUH/UoO Manotheepan, Ravinea PhD student, IEMR, OUH/UoO Melleby, Arne Olav PhD student, IEMR, OUH/UoO Nordén, Einar Sjaastad PhD student, IEMR, OUH/UoO Romaine, Andreas PhD student, IEMR, OUH/UoO Ruud, Marianne PhD student, IEMR, OUH/UoO Rypdal, Karoline Bjarnesdatter PhD student, IEMR, OUH/UoO Sadredini, Mani PhD student, IEMR, OUH/UoO Seljeflot, Ingebjørg Professor, Center for Clinical Heart Res., OUH Shen, Xin Pos tdoctoral fellow, IEMR, OUH/UoO Sjaastad, Ivar Professor, IEMR, UoO/OUH Skogestad, Jonas PhD student, IEMR, OUH/UoO Stensløkken, Kåre-Olav Professor, IMB, UoO Strand, Mari Elen Postdoctoral fellow, IEMR, OUH/UoO Veras, Ioanni Research associate, IEMR, OUH/UoO IEMR = Institute for Experimental Medical Research IMB = Institute of Medical Basic Sciences OUH = Oslo University Hospital UoO = University of Oslo Directions to Ullevål hospital The number 20 and 28 bus stop directly outside Ullevål hospital (the stop is called Ullevål sykehus). The hospital is also located next to a light rail station on the Ullevål Hageby Line of the Oslo Tramway. It is located on the south- western side of Ullevål University Hospital, at the intersection of Sognsveien and Kirkeveien. Tickets can be purchased via the Ruter app, at ticket booths or in Narvesen shops. On arriving at the hospital, follow the signs from the main entrance (building 4) to building 7 and climb the stairs to the 4th floor. Map of Ullevål hospital Visiting Oslo Oslo is home to many popular tourist attractions to be enjoyed in the Spring season. Sculpture park in the Frogner Park with more than 200 sculptures by Gustav Vigeland (1869–1943) in bronze, granite and cast iron, including The Angry Boy (Sinnataggen in Norwegian) Oslo's Opera House is located right at the harbour, with an angled, white exterior that appears to rise from the water. It invites its visitors to climb its roof and enjoy panoramic views of Oslo and the fjord, all year round. About NORHEART NORHEART – Norwegian PhD School of Heart Research NORHEART is a national education network for collaborative education of PhD students within cardiovascular research in Norway financed by The Research Council of Norway. NORHEART is initiated, organized and run by cardiovascular scientists. NORHEART is organized as a collaborative network with local nuclei in all university regions: Oslo, Tomsø, Trondheim, Bergen NORHEART events are continuously evolving to promote ongoing and future projects. Therefore all events are based on suggestions and initiatives from active scientists. Some of our upcoming events: Workshop Female Heart Workshop 2019 Solstrand Hotel, May 28-29 2019 This workshop focuses on sex – inflammation – cardiovascular disease. Workshop NORHEART Nature Masterclass 2019 – an interactive workshop Sola Strand Hotel, Stavanger, June 11-12 2019 Offers two days of interactive sessions and unique scientific feedback from editors in Nature Journals Symposium CHFR Annual Symposium 2019 Holmenkollen Park Hotel, Oslo, September 18–20, 2019 Invited lectures are given by international and national scientists on the topic of Heart Failure. Monday April 8th 15.05-16.05 Practical Demonstration 1: Methods in Molecular biology Location Demonstrator 15.10-15.25 15.30-15.45 15.50-16.05 Cell work IEMF Mari Elen 4th floor Strand PCR IEMF Almira Hasic 4th floor Western IEMF Marianne 4th floor Lunde Tuesday April 9h 10.45-12.00 Practical Demonstration 2: Assessment of Cardiac Function in Small Animals Location Demonstrator 11.00-11.15 11.20-11.35 11.40-11.55 Mouse surgery Build. 25 Arne Olav Melleby Echocardiography on Build. 25 Einar Sjaastad rats Nordén MR for small animal Build.
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