DOCSLIB.ORG

The Enveloping of Intercellular Collagenous Fibrils by Reticular Cell Processes in Postnatal Development of Rat Lymph Nodes

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Enveloping of Intercellular Collagenous Fibrils by Reticular Cell Processes in Postnatal Development of Rat Lymph Nodes Arch. Histol. Cytol., Vol. 55, No. 4 (1992) p. 351-359 The Enveloping of Intercellular Collagenous Fibrils by Reticular Cell Processes in Postnatal Development of Rat Lymph Nodes Toshiko YOSHIDA and Kenichi TAKAYA Department of Anatomy, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan Received July 20, 1991; revised manuscript June 30, 1992 Summary. In the lymph nodes of adult rats reticular SMIT et al., 1980; HENDRIKS et al., 1981). They are fibers are known to be covered by the processes of similar in their all having a close relation with reti- reticular cells. This study aims to visualize the sequence cular fibers (SORENSEN,1960; HAN, 1961; CLARK, 1962; of the envelopment of reticular fibers by reticular cells MOE, 1963; BAIRATI et al., 1964; MIYATA and TAKAYA, during development. Rat popliteal lymph nodes of one 1981, 1983; HAYAKAWA et al., 1988). Particularly, the to twenty-three days after birth were examined by electron microscopy. reticular cells in the lymph node sinus characteristi- At the earliest stages, collagenous protofibrils were cally enclose the reticular fibers with their own cyto- found in the intercellular space between studded mesen- plasmic processes whith have electron dense areas chymal cells. The protofibrils clustered around the beneath the membrane (MIYATA and TAKAYA, 1981, plasma membrane of immature reticular cells and then 1983, 1984, 1985). In the present study, the sequence of became arranged into microfibrils of 30-40nm in diame- enclosure of the reticular fiber by the reticular cell ter. Bundles of the fibrils which might be called reicular prosess was observed by electron microscopy of rat fibers were surrounded by processes of more than one lymph nodes in postnatal development. reticular cell. Then the reticular fiber came to be en- closed by the cytoplasmic process of a single reticular cell. Finally at 16-23 days after birth, the reticular fiber MATERIALS AND METHODS was completely ensheathed by the thick cytoplasmic process of a single reticular cell closed with a functional complex. Throughout these periods, basal lamina-like Wistar rats of both sexes aged 1-7, 9, 14, 16, 23 days materials existed between the reticular fiber and cyto- and 12 weeks were used for this study. Under Nem- plasmic process. Clumps of fibrils were rarely in contact butal anesthesia, the thorax was opened and the with leukocytes, including lymphocytes. Immature elas- blood was expelled by perfusion of physiological tic fibers appeared among collagenous fibrlis of the saline through the left ventricle. Perfusion fixation reticular fiber when the fiber came to be enclosed by with Karnovsky's fluid followed, and the popliteal processes of some reticular cells. lymph nodes of both sides were removed and refixed It was shown that the enclosure of the reticular fiber in the same fluid at 4°C for 4 h. The tissue blocks were by the reticular cell did not result from physical pres- rinsed in the phosphate buffer with the addition of sure due to the increase of the number of lymphocytes, 10% sucrose, then postfixed in 2% osmium tetroxide, but the reticular cell actively enclosed the reticular fiber. The reticular cell processes enveloping the reticu- and then stained en bloc in 3% aqueous uranyl ace- lar fiber were connected with desmosome-like function- tate solution. They were dehydrated through an al complexes to prevent the fiber from escaping. alcoholic series, cleared in propylene oxide and em- bedded in Quetol 812. Structures were confirmed in the 1, um semithin sections which were stained with Certain varieties of reticular cells have been found toluidine blue. Ultrathin sections were doubly stained in the sinus, cortex and medulla of the lymph node in uranyl acetate and lead citrate. They were (STUART, 1975; HOEFSMIT, 1975; KAMPERDIJKet al., examined under an electron microscope (JEM-1005) 1978; MULLER-HERMELINKand LENNERT, 1978; HOEF- at an acceleration voltage of 80 kV. 351 352 T. YOSHIDA and K. TAKAYA: 1 2 Fig. 1. Popliteal lymph node from a one-day-old rat. A mesenchymal cell (MC) is located near the capillary (C). The cytoplasm contains rough endoplasmic reticulum (rER). Collagenous protofibrils exist in the intercel- lular space (arrow). X4,100 Fig. 2. Popliteal lymph node from a one-day-old rat. Note incontinuous basal lamina (*) present on one side of a mesenchymal cell (MC) and the dense area (D) inside the plasma membrane. Protofibrils (arrows) are located in the intercellular spaces. x11,800 Fig. 3. Popliteal lymph node from a two-day-old rat. Clumps of protofibrils (Pf) in the intercellular spaces are attached to the plasma membrane of a mesenchymal cell in the area with dense material beneath it. In the cytoplasm, bundles of filaments (Fil) and a few mitochondria (mi), multivesicular bodies (mvb) and rough endoplasmic reticulum (rER) filled with flocculent substance are visible. v Vesicle x8,700 Fig. 4. Popliteal lymph node from a four-day-old rat. Several immature reticular cells (RC) are grouped to bridge capillaries (C) and constitute a reticulated network. A cell near the capillary extends its cytoplasmic process to form an arch with another cell process, thus surrounding the reticular fiber (Rf). A high magnification of the area is shown in Fig. 6. En endothelial cell. X3,900 Fig. 5. Popliteal lymph node from a five-day-old rat. The cytoplasm of an immature reticular cell extends its process to cover the reticular fiber (Rf) which consists of collagenous fibrils. A mass of microfibrils (arrows), probably of elastic fibers, is seen in the reticular fiber. No homogeneous substance is recognized yet. Note dilated cistern of rough endoplamic reticulum (rER) filled with flocculent substance. mi mitochondria, Ly lysosome x 10,000 Fig. 6. A higher magnification of the area of Rf in Figure 4. The reticular fiber (Rf) consisting of collagenous fibrils, 30-40 nm in diameter, is covered with the cytoplasmic processes of some immature reticular cells on one side and faced with an endothelial cell (En) on the other side. Elastic fibers (Ef) are constituted only of abundant microfibrils, or those surrounding amorphous materials (arrows). A basal lamina-like substance is seen outside the cell process plasma membrane facing the reticular fiber (arrowheads). X26,000 Reticular Fibers of the Lymph Node in Rat Postnatal Development 353 3 4 5 6 Figs. 3-6. Legends on the opposite page. 354 T. YOSHIDA and K. TAKAYA: 7 8 Fig. 7. Popliteal lymph node from a nine-day-old rat. The reticular fibers (Rf) consisting of collagenous and elastic elements are located among the processes of two or three cells. The fibers show close contact with the plasma membrane with dense areas inside it. RC reticular cell. x6,000 Fig. 8. Popliteal lymph node from a ten-day-old rat. Clumps of protofibrils (Pf) unite to form collagenous fibrils (Cf) in the intercellular space. They closely contact the cell membrane with the dense area (D) beneath it. Note distinct periodicity (arrows) on the collagenous fibrils. The fibrils are 40nm in diameter. mi Mitochondria. x11,000 Fig. 9. Popliteal lymph node from a 16-day-old rat. An immature reticular cell (RC) extends its cytoplasmic process (Cyp) toward the reticular fiber (Rf) to cover it (Cyp and arrows). No other cells-including lymphocyte (L)-come in contact with the reticular fiber. X 10,000 Fig. 10. Popliteal lymph node from a 16-day-old rat. The cytoplasmic process (Cyp and arrows) of a reticular cell (RC) form eaves around the fiber. Rf reticular fiber. x6,600 Fig. 11. Popliteal lymph node from a 23-day-old rat. Note the reticular fiber (Rf) completely surrounded by the cytoplasmic process (Cyp and arrows) of a reticular cell (RC). Dense material is seen inside the plasma membrane of the cell process facing the reticular fiber. The reticular cell attaches to the capillary (C) on one side and extends several cytoplasmic processes on the other. X6,000 Fig. 12. Another example of the reticular fiber (Rf) completely wrapped by its own cytoplasmic process of a single reticular cell seen in the lymph node from a ten-day-old rat. (The feature was quite rarely found in the lymph node at this developmental stage.) The reticular cell possesses junctional complexes (arrowheads) as if in order to prevent the fibers from escaping out of the enclosure. Elastic fibers (Ef) in the reticular fiber consist of only microfibrils and those surrounding amorphous materials. X 16,000 Fig. 13. In the lymph node of a 23-day-old rat, the cytoplasmic processes of the reticular cell are tightly connected with each other by a desmosome-like junctional complex (arrowheads). The reticular fiber (Rf) is completely enclosed. Note the basal lamina-like dense material outside the plasma membrane of the cell process facing the reticular fiber (Rf).x21,000 Reticular Fibers of the Lymph Node in Rat Postnatal Development 355 9 10 12 11 13 Figs. 9-13. Legends on the opposite page. 356 T. YOSHIDA and K. TAKAYA: increased in number and accumulated in the network. RESULTS The reticular cell in primary follicles enclosed the reticular fiber completely by its cytoplasmic process Subcapsular sinuses were present in the popliteal with junctional complexes (Fig. 12). However, in the lymph nodes of rats already on the first postnatal areas surrounding the follicles and in those develop- day. Cells lining the capsule and retothelial cells, ing into follicles, some presumptive reticular cells which were a type of reticular cell, enclosed the were extending their processes to wrap the reticular reticular fibers and made contact with each other by fibers (Figs. 7, 9), while others made eaves around the junctional complexes, as desctibed in the lymph node fibers (Fig. 10). In the area of developing follicles, sinus of the adult rat (MIYATA and TAKAYA, 1981).
Load more

Recommended publications
  • Te2, Part Iii
    TERMINOLOGIA EMBRYOLOGICA Second Edition International Embryological Terminology FIPAT The Federative International Programme for Anatomical Terminology A programme of the International Federation of Associations of Anatomists (IFAA) TE2, PART III Contents Caput V: Organogenesis Chapter 5: Organogenesis (continued) Systema respiratorium Respiratory system Systema urinarium Urinary system Systemata genitalia Genital systems Coeloma Coelom Glandulae endocrinae Endocrine glands Systema cardiovasculare Cardiovascular system Systema lymphoideum Lymphoid system Bibliographic Reference Citation: FIPAT. Terminologia Embryologica. 2nd ed. FIPAT.library.dal.ca. Federative International Programme for Anatomical Terminology, February 2017 Published pending approval by the General Assembly at the next Congress of IFAA (2019) Creative Commons License: The publication of Terminologia Embryologica is under a Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) license The individual terms in this terminology are within the public domain. Statements about terms being part of this international standard terminology should use the above bibliographic reference to cite this terminology. The unaltered PDF files of this terminology may be freely copied and distributed by users. IFAA member societies are authorized to publish translations of this terminology. Authors of other works that might be considered derivative should write to the Chair of FIPAT for permission to publish a derivative work. Caput V: ORGANOGENESIS Chapter 5: ORGANOGENESIS
    [Show full text]
  • Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
    Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal.
    [Show full text]
  • Basic Histology (23 Questions): Oral Histology (16 Questions
    Board Question Breakdown (Anatomic Sciences section) The Anatomic Sciences portion of part I of the Dental Board exams consists of 100 test items. They are broken up into the following distribution: Gross Anatomy (50 questions): Head - 28 questions broken down in this fashion: - Oral cavity - 6 questions - Extraoral structures - 12 questions - Osteology - 6 questions - TMJ and muscles of mastication - 4 questions Neck - 5 questions Upper Limb - 3 questions Thoracic cavity - 5 questions Abdominopelvic cavity - 2 questions Neuroanatomy (CNS, ANS +) - 7 questions Basic Histology (23 questions): Ultrastructure (cell organelles) - 4 questions Basic tissues - 4 questions Bone, cartilage & joints - 3 questions Lymphatic & circulatory systems - 3 questions Endocrine system - 2 questions Respiratory system - 1 question Gastrointestinal system - 3 questions Genitouirinary systems - (reproductive & urinary) 2 questions Integument - 1 question Oral Histology (16 questions): Tooth & supporting structures - 9 questions Soft oral tissues (including dentin) - 5 questions Temporomandibular joint - 2 questions Developmental Biology (11 questions): Osteogenesis (bone formation) - 2 questions Tooth development, eruption & movement - 4 questions General embryology - 2 questions 2 National Board Part 1: Review questions for histology/oral histology (Answers follow at the end) 1. Normally most of the circulating white blood cells are a. basophilic leukocytes b. monocytes c. lymphocytes d. eosinophilic leukocytes e. neutrophilic leukocytes 2. Blood platelets are products of a. osteoclasts b. basophils c. red blood cells d. plasma cells e. megakaryocytes 3. Bacteria are frequently ingested by a. neutrophilic leukocytes b. basophilic leukocytes c. mast cells d. small lymphocytes e. fibrocytes 4. It is believed that worn out red cells are normally destroyed in the spleen by a. neutrophils b.
    [Show full text]
  • Histology Histology
    HISTOLOGY HISTOLOGY ОДЕСЬКИЙ НАЦІОНАЛЬНИЙ МЕДИЧНИЙ УНІВЕРСИТЕТ THE ODESSA NATIONAL MEDICAL UNIVERSITY Áiáëiîòåêà ñòóäåíòà-ìåäèêà Medical Student’s Library Серія заснована в 1999 р. на честь 100-річчя Одеського державного медичного університету (1900–2000 рр.) The series is initiated in 1999 to mark the Centenary of the Odessa State Medical University (1900–2000) 1 L. V. Arnautova O. A. Ulyantseva HISTÎLÎGY A course of lectures A manual Odessa The Odessa National Medical University 2011 UDC 616-018: 378.16 BBC 28.8я73 Series “Medical Student’s Library” Initiated in 1999 Authors: L. V. Arnautova, O. A. Ulyantseva Reviewers: Professor V. I. Shepitko, MD, the head of the Department of Histology, Cytology and Embryology of the Ukrainian Medical Stomatologic Academy Professor O. Yu. Shapovalova, MD, the head of the Department of Histology, Cytology and Embryology of the Crimean State Medical University named after S. I. Georgiyevsky It is published according to the decision of the Central Coordinational Methodical Committee of the Odessa National Medical University Proceedings N1 from 22.09.2010 Навчальний посібник містить лекції з гістології, цитології та ембріології у відповідності до програми. Викладено матеріали теоретичного курсу по всіх темах загальної та спеціальної гістології та ембріології. Посібник призначений для підготовки студентів до практичних занять та ліцензійного екзамену “Крок-1”. Arnautova L. V. Histology. A course of lectures : a manual / L. V. Arnautova, O. A. Ulyantseva. — Оdessa : The Оdessa National Medical University, 2010. — 336 p. — (Series “Medical Student’s Library”). ISBN 978-966-443-034-7 The manual contains the lecture course on histology, cytology and embryol- ogy in correspondence with the program.
    [Show full text]
  • Nomina Histologica Veterinaria, First Edition
    NOMINA HISTOLOGICA VETERINARIA Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association of Veterinary Anatomists Published on the website of the World Association of Veterinary Anatomists www.wava-amav.org 2017 CONTENTS Introduction i Principles of term construction in N.H.V. iii Cytologia – Cytology 1 Textus epithelialis – Epithelial tissue 10 Textus connectivus – Connective tissue 13 Sanguis et Lympha – Blood and Lymph 17 Textus muscularis – Muscle tissue 19 Textus nervosus – Nerve tissue 20 Splanchnologia – Viscera 23 Systema digestorium – Digestive system 24 Systema respiratorium – Respiratory system 32 Systema urinarium – Urinary system 35 Organa genitalia masculina – Male genital system 38 Organa genitalia feminina – Female genital system 42 Systema endocrinum – Endocrine system 45 Systema cardiovasculare et lymphaticum [Angiologia] – Cardiovascular and lymphatic system 47 Systema nervosum – Nervous system 52 Receptores sensorii et Organa sensuum – Sensory receptors and Sense organs 58 Integumentum – Integument 64 INTRODUCTION The preparations leading to the publication of the present first edition of the Nomina Histologica Veterinaria has a long history spanning more than 50 years. Under the auspices of the World Association of Veterinary Anatomists (W.A.V.A.), the International Committee on Veterinary Anatomical Nomenclature (I.C.V.A.N.) appointed in Giessen, 1965, a Subcommittee on Histology and Embryology which started a working relation with the Subcommittee on Histology of the former International Anatomical Nomenclature Committee. In Mexico City, 1971, this Subcommittee presented a document entitled Nomina Histologica Veterinaria: A Working Draft as a basis for the continued work of the newly-appointed Subcommittee on Histological Nomenclature. This resulted in the editing of the Nomina Histologica Veterinaria: A Working Draft II (Toulouse, 1974), followed by preparations for publication of a Nomina Histologica Veterinaria.
    [Show full text]
  • Índice De Denominacións Españolas
    VOCABULARIO Índice de denominacións españolas 255 VOCABULARIO 256 VOCABULARIO agente tensioactivo pulmonar, 2441 A agranulocito, 32 abaxial, 3 agujero aórtico, 1317 abertura pupilar, 6 agujero de la vena cava, 1178 abierto de atrás, 4 agujero dental inferior, 1179 abierto de delante, 5 agujero magno, 1182 ablación, 1717 agujero mandibular, 1179 abomaso, 7 agujero mentoniano, 1180 acetábulo, 10 agujero obturado, 1181 ácido biliar, 11 agujero occipital, 1182 ácido desoxirribonucleico, 12 agujero oval, 1183 ácido desoxirribonucleico agujero sacro, 1184 nucleosómico, 28 agujero vertebral, 1185 ácido nucleico, 13 aire, 1560 ácido ribonucleico, 14 ala, 1 ácido ribonucleico mensajero, 167 ala de la nariz, 2 ácido ribonucleico ribosómico, 168 alantoamnios, 33 acino hepático, 15 alantoides, 34 acorne, 16 albardado, 35 acostarse, 850 albugínea, 2574 acromático, 17 aldosterona, 36 acromatina, 18 almohadilla, 38 acromion, 19 almohadilla carpiana, 39 acrosoma, 20 almohadilla córnea, 40 ACTH, 1335 almohadilla dental, 41 actina, 21 almohadilla dentaria, 41 actina F, 22 almohadilla digital, 42 actina G, 23 almohadilla metacarpiana, 43 actitud, 24 almohadilla metatarsiana, 44 acueducto cerebral, 25 almohadilla tarsiana, 45 acueducto de Silvio, 25 alocórtex, 46 acueducto mesencefálico, 25 alto de cola, 2260 adamantoblasto, 59 altura a la punta de la espalda, 56 adenohipófisis, 26 altura anterior de la espalda, 56 ADH, 1336 altura del esternón, 47 adipocito, 27 altura del pecho, 48 ADN, 12 altura del tórax, 48 ADN nucleosómico, 28 alunarado, 49 ADNn, 28
    [Show full text]
  • Reticulum Cell Sarcoma of Lymph Node with Mixed Dendritic and Fibroblastic Features Dan Jones, M.D., Ph.D., Mitual Amin, M.D., Nelson G
    Reticulum Cell Sarcoma of Lymph Node with Mixed Dendritic and Fibroblastic Features Dan Jones, M.D., Ph.D., Mitual Amin, M.D., Nelson G. Ordonez, M.D., Armand B. Glassman, M.D., Kimberly J. Hayes, B.S., L. Jeffrey Medeiros, M.D. Division of Pathology and Laboratory Medicine, University of Texas-M.D. Anderson Cancer Center, Houston, Texas Lymph nodes contain a heterogeneous population We report a case of clinically aggressive reticulum of stromal cells with reticular morphology. These cell sarcoma with mixed follicular dendritic cell include antigen-presenting follicular dendritic cells (FDC) and fibroblastic reticular cell (FRC) features. (FDC) within the lymphoid follicle; interdigitating Histologically, the tumor was confined to lymph reticular cells (IDCs) related to the myeloid/mono- nodes occurring as a multifocal epithelioid and cytic lineage, and interfollicular fibroblastic reticu- spindle cell proliferation with appreciable mitotic lar cells (FRCs) of mesenchymal origin (1–4). The rate and numerous admixed non-neoplastic B-cells. FDC population includes multiple immunopheno- Ultrastructural examination revealed elongated typically distinct subsets of stromal cells within the cells with prominent nucleoli, interdigitating cell lymphoid follicle and mantle zone that regulate processes and frequent desmosomes. These fea- distinct stages of B cell differentiation (5, 6). IDCs tures are typical of FDC sarcoma. However, immu- have a primary function in antigen presentation in nohistochemical stains showed no expression of an- the interfollicular zones of the node and differenti- tigens characteristic of FDCs, including CD21, CD23 ate from Langerhans cells migrating from skin or and CD35. Cytogenetic characterization of this tu- from bone marrow-derived precursors (2).
    [Show full text]
  • 6189.Full.Pdf
    Organizer-Like Reticular Stromal Cell Layer Common to Adult Secondary Lymphoid Organs This information is current as Tomoya Katakai, Hidenori Suto, Manabu Sugai, Hiroyuki of September 25, 2021. Gonda, Atsushi Togawa, Sachiko Suematsu, Yukihiko Ebisuno, Koko Katagiri, Tatsuo Kinashi and Akira Shimizu J Immunol 2008; 181:6189-6200; ; doi: 10.4049/jimmunol.181.9.6189 http://www.jimmunol.org/content/181/9/6189 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2008/10/17/181.9.6189.DC1 Material http://www.jimmunol.org/ References This article cites 57 articles, 21 of which you can access for free at: http://www.jimmunol.org/content/181/9/6189.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision by guest on September 25, 2021 • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2008 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Organizer-Like Reticular Stromal Cell Layer Common to Adult Secondary Lymphoid Organs1 Tomoya Katakai,2*‡ʈ Hidenori Suto,*† Manabu Sugai,*‡ Hiroyuki Gonda,*‡ Atsushi Togawa,§ Sachiko Suematsu,¶ Yukihiko Ebisuno,ʈ Koko Katagiri,ʈ Tatsuo Kinashi,ʈ and Akira Shimizu*†‡ Mesenchymal stromal cells are crucial components of secondary lymphoid organs (SLOs).
    [Show full text]
  • Papillary and Reticular Fibroblasts Generate Distinct Microenvironments
    bioRxiv preprint doi: https://doi.org/10.1101/2020.11.29.402594; this version posted November 29, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. PAPILLARY AND RETICULAR FIBROBLASTS GENERATE DISTINCT MICROENVIRONMENTS THAT DIFFERENTIALLY IMPACT ANGIOGENESIS Adèle Mauroux1,2,3, Pauline Joncour2, Benjamin Gillet2, Sandrine Hughes2, Corinne Ardidie- Robouant1, Laëtitia Marchand3, Athanasia Liabotis1, Philippe Mailly1, Catherine Monnot1, Stephane Germain1, Sylvie Bordes3, Brigitte Closs3, Florence Ruggiero 2†*, Laurent Muller 1†* 1: Center for Interdisciplinary Research in Biology (CIRB), College de France - Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Paris Sciences et Lettres (PSL) Research University, Paris, France. 2: Institut de Génomique Fonctionnelle de Lyon,ENS de Lyon, CNRS, Univ Lyon 1, Lyon,France; 3: R&D Department, SILAB, Brive,France * These authors contributed equally to this work † Corresponding authors Corresponding authors e-mail: [email protected] ; Laurent.muller@college-de- france.fr 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.11.29.402594; this version posted November 29, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ABSTRACT Papillary and reticular dermis show distinct extracellular matrix (ECM) and vascularization, and fibroblasts isolated from these compartments have different gene expression patterns and behaviour in vitro. However, due to lack of relevant models, the contribution of skin fibroblast sub-populations to vascularization remains unknown.
    [Show full text]
  • LN-Derived Fibroblastic Reticular Cells and Their Impact on T Cell Response—A Systematic Review
    cells Review LN-Derived Fibroblastic Reticular Cells and Their Impact on T Cell Response—A Systematic Review Bianca O. Ferreira 1,† , Lionel F. Gamarra 1,†, Mariana P. Nucci 1,2 , Fernando A. Oliveira 1 , Gabriel N. A. Rego 1 and Luciana Marti 1,* 1 Department of Experimental Research, Hospital Israelita Albert Einstein, São Paulo 05652-000, Brazil; [email protected] (B.O.F.); [email protected] (L.F.G.); [email protected] (M.P.N.); [email protected] (F.A.O.); [email protected] (G.N.A.R.) 2 LIM44—Hospital das Clínicas da Faculdade Medicina da Universidade de São Paulo, São Paulo 05403-000, Brazil * Correspondence: [email protected]; Tel.: +55-11-2151-1353 † These authors contributed equally to this work. Abstract: Fibroblastic reticular cells (FRCs), usually found and isolated from the T cell zone of lymph nodes, have recently been described as much more than simple structural cells. Originally, these cells were described to form a conduit system called the “reticular fiber network” and for being responsible for transferring the lymph fluid drained from tissues through afferent lymphatic vessels to the T cell zone. However, nowadays, these cells are described as being capable of secreting several cytokines and chemokines and possessing the ability to interfere with the immune response, improving it, and also controlling lymphocyte proliferation. Here, we performed a systematic review of the several methods employed to investigate the mechanisms used by fibroblastic reticular cells to Citation: Ferreira, B.O.; Gamarra, control the immune response, as well as their ability in determining the fate of T cells.
    [Show full text]
  • 26 April 2010 TE Prepublication Page 1 Nomina Generalia General Terms
    26 April 2010 TE PrePublication Page 1 Nomina generalia General terms E1.0.0.0.0.0.1 Modus reproductionis Reproductive mode E1.0.0.0.0.0.2 Reproductio sexualis Sexual reproduction E1.0.0.0.0.0.3 Viviparitas Viviparity E1.0.0.0.0.0.4 Heterogamia Heterogamy E1.0.0.0.0.0.5 Endogamia Endogamy E1.0.0.0.0.0.6 Sequentia reproductionis Reproductive sequence E1.0.0.0.0.0.7 Ovulatio Ovulation E1.0.0.0.0.0.8 Erectio Erection E1.0.0.0.0.0.9 Coitus Coitus; Sexual intercourse E1.0.0.0.0.0.10 Ejaculatio1 Ejaculation E1.0.0.0.0.0.11 Emissio Emission E1.0.0.0.0.0.12 Ejaculatio vera Ejaculation proper E1.0.0.0.0.0.13 Semen Semen; Ejaculate E1.0.0.0.0.0.14 Inseminatio Insemination E1.0.0.0.0.0.15 Fertilisatio Fertilization E1.0.0.0.0.0.16 Fecundatio Fecundation; Impregnation E1.0.0.0.0.0.17 Superfecundatio Superfecundation E1.0.0.0.0.0.18 Superimpregnatio Superimpregnation E1.0.0.0.0.0.19 Superfetatio Superfetation E1.0.0.0.0.0.20 Ontogenesis Ontogeny E1.0.0.0.0.0.21 Ontogenesis praenatalis Prenatal ontogeny E1.0.0.0.0.0.22 Tempus praenatale; Tempus gestationis Prenatal period; Gestation period E1.0.0.0.0.0.23 Vita praenatalis Prenatal life E1.0.0.0.0.0.24 Vita intrauterina Intra-uterine life E1.0.0.0.0.0.25 Embryogenesis2 Embryogenesis; Embryogeny E1.0.0.0.0.0.26 Fetogenesis3 Fetogenesis E1.0.0.0.0.0.27 Tempus natale Birth period E1.0.0.0.0.0.28 Ontogenesis postnatalis Postnatal ontogeny E1.0.0.0.0.0.29 Vita postnatalis Postnatal life E1.0.1.0.0.0.1 Mensurae embryonicae et fetales4 Embryonic and fetal measurements E1.0.1.0.0.0.2 Aetas a fecundatione5 Fertilization
    [Show full text]
  • Electron Microscopic Studies of the Differentiation of Fat Cells in Human Fetal Skin
    THE JOURNAL OP INVESTIGATIVE DERMATOLOGY Vol. 53, No. 2 Copyright C 1959 by The Williams & Wilkins Co. Printed in U.S.A. ELECTRON MICROSCOPIC STUDIES OF THE DIFFERENTIATION OF FAT CELLS IN HUMAN FETAL SKIN* HIDESUKE FUJITA, M.D., CHIDORI ASAGAMI, M.D., YOSHIHIKO ODA, M.D., TAKASHI MORI, M.D. AND YOSHIKO SUETOMI. M.D. ABSTRACT 1) Electron microscopic studies of primitive fat organs showing various degrees of fat storage from human fetuses 20 to 26 weeks of age were undertaken, with special reference to the origin of fat cells and the lipid formations in them. Based on this study, three types of cells, namely an undifferentiated-type cell, a young-type fat cell and a mature- type fat cell were discerned ultrastructurally, but they are considered to be identical con- cerning the morphology of mitochondria, smooth endoplasmic reticula and glycogen granules. 2) Concerning the origin of white fat cells, it was revealed that young-type and mature- type fat cells are not derived from reticuloendothelial cells or fibroblasts but from a certain definite type of mesenchymal cell which we have referred to as the undifferentiated-type cell. 3) The finding that cytoplasmic microvesicles are more prominent in young-type fat cells than in mature-type ones might be interpreted that the young-type cells may be actively releasing lipids or free fatty acids according to Williamson's concept of these organelles. But, contrary to Williamson's postulation, the young-type fat cells seem to be accumulating lipids or free fatty acids. Despite previous embryological studies on RESULTS the differentiation of fat cells in human skin made by light microscopy, important prob- Light Microscopy lems still remain unsolved (1—5).
    [Show full text]