Electrochemical and Molecular Characterization of Electroactive Biofilms on Stainless Steel in Marine Environment Florian Trigodet

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Electrochemical and Molecular Characterization of Electroactive Biofilms on Stainless Steel in Marine Environment Florian Trigodet Electrochemical and molecular characterization of electroactive biofilms on stainless steel in marine environment Florian Trigodet To cite this version: Florian Trigodet. Electrochemical and molecular characterization of electroactive biofilms on stainless steel in marine environment. Microbiology and Parasitology. Université de Bretagne occidentale - Brest, 2019. English. NNT : 2019BRES0029. tel-02307295 HAL Id: tel-02307295 https://tel.archives-ouvertes.fr/tel-02307295 Submitted on 7 Oct 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. @74$4%34%3'2@')0@%34 % % % 9,A.804)$8@4 %% 34 %1)[email protected] %'22834.@094 % 2'3A4% A.804)$8@4% 1)[email protected]% 9'8)4 % 42'94% 3'2@')094%. f%5.7 %% !$($%$$-'($')"' $,'$.'(.')(../,") '% $TaGMEPMXa%/% ($,/0(/)/1($ ! REB% E,H$D#G!75-HI()7 !! "#$#%MU$DL#MDHG!U,-%M$H%.D/D01-!-M!/H,U%1,#D$-!2-L!3DHAD,/L! U,-%M$H#%MDAL!L1$!#%D-$!DGH5R2#3,-!-G!/D,D-1!/#$DG % % % 7.8L-!I$UL-GMU-!-M!LH1M-G1-!T!4,H1S<GU=!,-!>?!#O$D,!A >?! 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  • Microbial Issues Encountered in Wastewater Treatment at Moorhead Factory and Remedial Measures
    MICROBIAL ISSUES ENCOUNTERED IN WASTEWATER TREATMENT AT MOORHEAD FACTORY AND REMEDIAL MEASURES Indrani S. Samaraweera*1, Terry D. McGillivray1, Diane L. Rheault1 and Dennis Burthwick2 American Crystal Sugar Company, Technical Services Center 11700 N. 11th Street and 22500 N. 11th Street, Moorhead, MN 56560 Introduction: Wastewater treatment is an integral part of processing of sugar beets in the sugar industry. American Crystal Sugar Company (ACS) has five factories. Three of these factories, Moorhead (MHD), Hillsboro (HLB), and East Grand Forks (EGF) each have a 6.7 million gallon anaerobic contactor, aerobic basin, and ponds for processing of wastewater while the other two factories have lagoons and wetlands for the treatment of their wastewater. During the 2007/2008 campaign our efforts were focused on microbial issues in wastewater treatment at the MHD factory. Therefore, this paper deals with problems encountered with filamentous bacteria, poor settling in treatment of the high strength wastewater and studies to circumvent these problems. In addition some differences observed in the MHD and HLB anaerobic systems will also be discussed. Materials and Methods: A) Microbiology 1) Sample collection Weekly samples of wastewater were obtained aseptically in sterile screw cap containers from each of three locations: a) anaerobic influent from the new covered wastewater pond, b) anaerobic tank or anaerobic contactor, and c) aerobic basin or activated sludge system. These samples were observed microscopically at the ACS Technical Services Center Microbiology Lab. Samples from similar locations in the wastewater treatment systems at the Hillsboro and East Grand Forks factories were intermittently obtained for comparative purposes. 2) Microscopy and Photography i) Wet Mounts, Staining, Floc formation and Higher Life Forms – Separate wastewater (WW) wet mounts on slides were observed microscopically with or without a drop of lactophenol cotton blue and/or India ink stain.
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  • Staining the Purpose of Staining : We Stain Bacteria to Study There : A) Morphology and Arrangement
    Staining The purpose of Staining : We stain bacteria to study there : A) Morphology and Arrangement . B)Differentiated bacteria to groups according to their biochemical composition of cell wall . C)Study structures of bacteria (capsule,flagella) . Stains are classified according to their functions into : 1)Simple stain (methylene blue , safranin) that help to stain the outlines of bacterial cells, giving one the characteristic shape ,size, and arrangement of the cells stained with the simple stain . 2)Differential stain (Gram stain ,acid fast stain) Differential stain will Differentiate between the two cells . 3)Special stains (capsule stain,flagella stain ) stained some structures of bacteria . Preparation of smear : 1. Clean the slide . 2. Place a loop full of water in the center of the slide . 3. Mix a small amount of bacteria using a loop with the water and spread it out . 4. Allow the slide to air dry. 5-Heat-fix the smear by passing the slide through the Benzen burner . 1)Simple stain : methylene blue stain 1. Prepare the smear . 2. Flood the slide with methylene blue stain for 3 mints . 3. Wash the slide with tap water gently,drain off excess water then let the slide dry in air or by using filter paper . 4. Exam it microscopically . 5. The bacteria will appear blue cells . 2) Differential stains : A)Gram staining . B)Ziehl Neelsen (Acid Fast ) staining : Is a differential stain used to identify acid-fast organisms as members of the genus Mycobacterium . Acid-fast organisms are characterized by wax-like,nearly impermeable cell walls;they contain mycolic acid and large amounts of fatty acids ,waxes,and complex lipids .
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  • Peptide Mediated Inhibition of Porphyromonas Gingivalis Dual and Three Species Biofilms
    University of Louisville ThinkIR: The University of Louisville's Institutional Repository Electronic Theses and Dissertations 12-2013 Peptide mediated inhibition of Porphyromonas gingivalis dual and three species biofilms. Naga Srinija Gummadi University of Louisville Follow this and additional works at: https://ir.library.louisville.edu/etd Recommended Citation Gummadi, Naga Srinija, "Peptide mediated inhibition of Porphyromonas gingivalis dual and three species biofilms." (2013). Electronic Theses and Dissertations. Paper 545. https://doi.org/10.18297/etd/545 This Master's Thesis is brought to you for free and open access by ThinkIR: The University of Louisville's Institutional Repository. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of ThinkIR: The University of Louisville's Institutional Repository. This title appears here courtesy of the author, who has retained all other copyrights. For more information, please contact [email protected]. PEPTIDE MEDIATED INHIBITION OF PORPHYROMONAS GINGIVALIS DUAL AND THREE SPECIES BIOFILMS By: Naga Srinija Gummadi University of Louisville School of Dentistry A Thesis Submitted to the Faculty Of the School of Dentistry of the University of Louisville in Partial Fulfillment of the Requirements for the Degree of Master of Science Oral Biology School of Dentistry University of Louisville Louisville, KY December 2013 Copyright by Naga Srinija Gummadi in 2013 All Rights Reserved PEPTIDE MEDIATED INHIBITION OF PORPHYROMONAS GINGIVALIS DUAL AND THREE SPECIES BIOFILMS By: Naga Srinija Gummadi, B.D.S., Dr. N.T.R University of Health Sciences, 2009 Thesis Approved on November 27, 2013 By the following Thesis Committee ______________________________ Dr. Donald R. Demuth, Ph.D.
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  • Review of Microbially Influenced Corrosion of High-Level Waste
    CNWRA 93-014 A S S. l ' -S I& 0X- 0,,,, al-s~~~~~~~~~~~ _ Prepared for Nuclear Regulatory Commission Contract NRC-02-88-005 Prepared by Center for Nuclear Waste Regulatory Analyses San Antonio, Texas June 1993 CNWRA 93-014 A REVIEW OF THE POTENTIAL FOR MICROBIALLY INFLUENCED CORROSION OF HIGH-LEVEL NUCLEAR WASTE CONTAINERS Prepared for Nuclear Regulatory Commission Contract NRC-02-88-005 Prepared by Gill Geesey Department of Microbiology Montana State University Bozeman, Montana Edited by Gustavo A. Cragnolino Center for Nuclear Waste Regulatory Analyses San Antonio, Texas June 1993 RECEIVED JUN 281993 CNWRA-WO PREVIOUS REPORTS IN SERIES Number Name Date Issued CNWRA 91-004 A Review of Localized Corrosion of High-Level Nuclear Waste Container Materials - I April 1991 CNWRA 91-008 Hydrogen Embrittlement of Candidate Container Materials June 1991 CNWRA 92-021 A Review of Stress Corrosion Cracking of High-Level Nuclear Waste Container Materials - I August 1992 CNWRA 93-003 Long-Term Stability of High-Level Nuclear Waste Container Materials: I - Thermal Stability of Alloy 825 February 1993 CNWRA 93-004 Experimental Investigations of Localized Corrosion of High-Level Waste Container Materials February 1993 ii ABSTRACT The potential for microbially influenced corrosion (MIC) of the candidate and alternate container materials for the proposed Yucca Mountain repository site is examined on the basis of an extensive review of the literature. A brief description of the environmental conditions expected outside the waste packages, in terms of the geology, hydrology, water chemistry, radiation, temperature, and moisture content, is followed by a detailed discussion regarding the characteristics of microbial life in subsurface environments.
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  • Prokaryotic Cell Features
    Prokaryotic Cell Features Size The small size of prokaryotic cells affects their physiology, growth rate, and ecology. Due to their small cell size, most prokaryotes have the highest surface area–to–volume ratio of any cells. This characteristic aids in nutrient and waste exchange with the environment. As a cell increases in size, its surface area-to-volume ratio decreases For a sphere, S / V = 3/r Prokaryotes maintain a high surface area to volume ratio by their smallness. 1. TRANSPORT RATE: Efficient transport of raw materials in and wastes out 2. GROWTH RATE: Nutrient exchange limits growth rates. Many prokaryotes = high metabolic activity (fast growth, reproductive rate). 3. EVOLUTIONARY RATE: Given the same amount of nutrients, small cells can have more individuals in a population than larger cells. Thus, more cell division, and more mutation, more evolutionary change. Eukaryotes larger cell size (in general) Different answers for the transport problem 1. lots of internal membrane channels, invaginations and surface area 2. cytoplasmic streaming 3. organelles (compartmentalize cell functions) 4. bulk uptake - endocytosis (phagocytosis/pinocytosis) Introducing a prokaryote that is one million times bigger than E. coli! Sturgeonfish symbiont. Epulopiscium fiseloni 600 µm (0.6 mm) long 20 - 40 µm long Individual flagella twist together to form “thick” flagella Largest known bacterium: Thiomargarita namibiensis Some nearly 1 mm wide Large central vacuole (dark) contains nitrate sequestered to use in the oxidation of reduced sulfur compounds. String of pearls: reflective sulfur granules How do we see microbes? 1. Light Microscopy Up to 1,500 X magnification (yours in lab: 1000 X) Limit of resolving power is ~0.2 µM, or about 1/3 the width of an average bacterial cell Light gathering ability decreases as magnification increases Immersion oil helps gather light rays that would otherwise be lost from specimen How do we see microbes? 2.
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  • Algal and Cyanobacterial Saline Biofilms of the Grande Coastal Lagoon, Lima, Peru
    Natural Resources and Environmental Issues Volume 15 Saline Lakes Around the World: Unique Systems with Unique Values Article 23 2009 Algal and cyanobacterial saline biofilms of the Grande Coastal Lagoon, Lima, Peru Haydee Montoya Natural History Museum, UNMSM, and Biological Sciences, Ricardo Palma University, Lima, Peru Follow this and additional works at: https://digitalcommons.usu.edu/nrei Recommended Citation Montoya, Haydee (2009) "Algal and cyanobacterial saline biofilms of the Grande Coastal Lagoon, Lima, Peru," Natural Resources and Environmental Issues: Vol. 15 , Article 23. Available at: https://digitalcommons.usu.edu/nrei/vol15/iss1/23 This Article is brought to you for free and open access by the Journals at DigitalCommons@USU. It has been accepted for inclusion in Natural Resources and Environmental Issues by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. Montoya: Algal and cyanobacterial biofilms of the Grande Coastal Lagoon Algal and Cyanobacterial Saline Biofilms of the Grande Coastal Lagoon, Lima, Peru Haydee Montoya1 1Natural History Museum, UNMSM, and Biological Sciences Faculty, Ricardo Palma University, Av. Arenales 1256, Apartado 14-0434, Lima 14, Perú, E-mail: [email protected] ABSTRACT coexist at an interface, with a distinct macromolecular matrix typically attached to a surface in which complex Tropical coastal wetland ecosystems are widely distributed food webs occur (Davey & O’Toole 2000; Larson & Passy in arid regions. The Grande coastal lagoon in Peru’s central 2005; De Vicente et al. 2006). Photosynthetic activity by plain is shallow, eutrophic and alkaline, exposed to the benthic microalgae is the primary source of fixed carbon in annual hydrological regime with flooding and desiccation shallow aquatic ecosystems.
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