Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds Mohib Abdullah (
[email protected] ) Jagiellonian University: Uniwersytet Jagiellonski w Krakowie https://orcid.org/0000-0002-4078-3754 Elwira Sliwinska UTP: Uniwersytet Technologiczno-Przyrodniczy im Jana i Jedrzeja Sniadeckich w Bydgoszczy Grzegorz Góralski Jagiellonian University: Uniwersytet Jagiellonski w Krakowie Piotr Latocha Warsaw University of Life Sciences: Szkola Glowna Gospodarstwa Wiejskiego w Warszawie Monika Tuleja Jagiellonian University: Uniwersytet Jagiellonski w Krakowie Patrycja Widyna Jagiellonian University: Uniwersytet Jagiellonski w Krakowie Marzena Popielarska-Konieczna Jagiellonian University: Uniwersytet Jagiellonski w Krakowie Research Article Keywords: callus, ow cytometry, histology, meta-topolin, ultrastructure, zeatin Posted Date: May 17th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-495183/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Version of Record: A version of this preprint was published at Plant Cell, Tissue and Organ Culture (PCTOC) on August 18th, 2021. See the published version at https://doi.org/10.1007/s11240-021-02149-5. Page 1/16 Abstract Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, a protocol of plant regeneration and acclimatization from the endosperm- derived calli of Actinidia arguta has been developed. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli.