Detection, Distribution and Migration of the Toxin in Aquatic Systems
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Echinoidea) Using Culture-Independent Methods
Journal of Invertebrate Pathology 100 (2009) 127–130 Contents lists available at ScienceDirect Journal of Invertebrate Pathology journal homepage: www.elsevier.com/locate/yjipa Short Communication Characterization of the bacterial community associated with body wall lesions of Tripneustes gratilla (Echinoidea) using culture-independent methods Pierre T. Becker a,*, David C. Gillan b, Igor Eeckhaut a,* a Laboratoire de biologie marine, Université de Mons-Hainaut, 6 Avenue du Champ de Mars, 7000 Mons, Belgium b Laboratoire de biologie marine, CP160/15, Université Libre de Bruxelles, 50 Avenue F. D. Roosevelt, 1050 Bruxelles, Belgium article info abstract Article history: The bacterial community associated with skin lesions of the sea urchin Tripneustes gratilla was investi- Received 17 July 2008 gated using 16S ribosomal RNA gene cloning and fluorescent in situ hybridization (FISH). All clones were Accepted 5 November 2008 classified in the Alphaproteobacteria, Gammaproteobacteria and Cytophaga–Flexibacter–Bacteroides (CFB) Available online 11 November 2008 bacteria. Most of the Alphaproteobacteria were related to the Roseobacter lineage and to bacteria impli- cated in marine diseases. The majority of the Gammaproteobacteria were identified as Vibrio while CFB Keywords: represented only 9% of the total clones. FISH analyses showed that Alphaproteobacteria, CFB bacteria Tripneustes gratilla and Gammaproteobacteria accounted respectively for 43%, 38% and 19% of the DAPI counts. The impor- Lesions tance of the methods used is emphasized. Cloning FISH Ó 2009 Published by Elsevier Inc. Sea urchin Bacterial infection 1. Introduction healthy echinoids (Becker et al., 2007). In the present study, a cul- ture-independent method (16S rRNA gene cloning) is used in order Body wall lesions consisting of infected areas of the test with to obtain a thorough identification of the bacterial community loss of epidermis and appendages are recurrently observed in associated with T. -
Fish and Fishery Products Hazards and Controls Guidance Fourth Edition – APRIL 2011
SGR 129 Fish and Fishery Products Hazards and Controls Guidance Fourth Edition – APRIL 2011 DEPARTMENT OF HEALTH AND HUMAN SERVICES PUBLIC HEALTH SERVICE FOOD AND DRUG ADMINISTRATION CENTER FOR FOOD SAFETY AND APPLIED NUTRITION OFFICE OF FOOD SAFETY Fish and Fishery Products Hazards and Controls Guidance Fourth Edition – April 2011 Additional copies may be purchased from: Florida Sea Grant IFAS - Extension Bookstore University of Florida P.O. Box 110011 Gainesville, FL 32611-0011 (800) 226-1764 Or www.ifasbooks.com Or you may download a copy from: http://www.fda.gov/FoodGuidances You may submit electronic or written comments regarding this guidance at any time. Submit electronic comments to http://www.regulations. gov. Submit written comments to the Division of Dockets Management (HFA-305), Food and Drug Administration, 5630 Fishers Lane, Rm. 1061, Rockville, MD 20852. All comments should be identified with the docket number listed in the notice of availability that publishes in the Federal Register. U.S. Department of Health and Human Services Food and Drug Administration Center for Food Safety and Applied Nutrition (240) 402-2300 April 2011 Table of Contents: Fish and Fishery Products Hazards and Controls Guidance • Guidance for the Industry: Fish and Fishery Products Hazards and Controls Guidance ................................ 1 • CHAPTER 1: General Information .......................................................................................................19 • CHAPTER 2: Conducting a Hazard Analysis and Developing a HACCP Plan -
1 Exon Probe Sets and Bioinformatics Pipelines for All Levels of Fish Phylogenomics
bioRxiv preprint doi: https://doi.org/10.1101/2020.02.18.949735; this version posted February 19, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Exon probe sets and bioinformatics pipelines for all levels of fish phylogenomics 2 3 Lily C. Hughes1,2,3,*, Guillermo Ortí1,3, Hadeel Saad1, Chenhong Li4, William T. White5, Carole 4 C. Baldwin3, Keith A. Crandall1,2, Dahiana Arcila3,6,7, and Ricardo Betancur-R.7 5 6 1 Department of Biological Sciences, George Washington University, Washington, D.C., U.S.A. 7 2 Computational Biology Institute, Milken Institute of Public Health, George Washington 8 University, Washington, D.C., U.S.A. 9 3 Department of Vertebrate Zoology, National Museum of Natural History, Smithsonian 10 Institution, Washington, D.C., U.S.A. 11 4 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, China 12 5 CSIRO Australian National Fish Collection, National Research Collections of Australia, 13 Hobart, TAS, Australia 14 6 Sam Noble Oklahoma Museum of Natural History, Norman, O.K., U.S.A. 15 7 Department of Biology, University of Oklahoma, Norman, O.K., U.S.A. 16 17 *Corresponding author: Lily C. Hughes, [email protected]. 18 Current address: Department of Organismal Biology and Anatomy, University of Chicago, 19 Chicago, IL. 20 21 Keywords: Actinopterygii, Protein coding, Systematics, Phylogenetics, Evolution, Target 22 capture 23 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.02.18.949735; this version posted February 19, 2020. -
Molecular Interaction Between Fish Pathogens and Host Aquatic Animals
K. Tsukamoto, T. Kawamura, T. Takeuchi, T. D. Beard, Jr. and M. J. Kaiser, eds. Fisheries for Global Welfare and Environment, 5th World Fisheries Congress 2008, pp. 277–288. © by TERRAPUB 2008. Molecular Interaction between Fish Pathogens and Host Aquatic Animals Laura L. Brown* and Stewart C. Johnson National Research Council of Canada Institute for Marine Biosciences 1411 Oxford Street Halifax, NS, B3H 3Z1, Canada Present address: Fisheries and Oceans Canada Pacific Biological Station 3190 Hammond Bay Road Nanaimo, NS, V9T 6N7, Canada *E-mail: [email protected] We have studied the host-pathogen interactions between Atlantic salmon (Salmo salar L.) and Aeromonas salmonicida. Sequencing the genome of the bacterium allowed us to investigate virulence factors and other gene products with potential as vaccines. Using knock-out mutants of A. salmonicida, we identified key virulence factors. Proteomics studies of bacterial cells grown in a variety of media as well as in an in vivo implant system revealed differential protein production and have shed new light on bacterial proteins such as superoxide dismutase, pili and flagellar proteins, type three secretion systems, and their roles in A. salmonicida pathogenicity. We constructed a whole ge- nome DNA microarray to use in comparative genomic hybridizations (M-CGH) and bacterial gene expression studies. Carbohydrate analysis has shown the variation in LPS between strains and reveals the importance of LPS in viru- lence. Salmon were challenged with A. salmonicida and tissues were taken to construct suppressive subtractive hybridization libraries to investigate differ- ential host gene expression. We constructed an Atlantic salmon cDNA microarray to investigate the host response to A. -
Chemical Pollution of the Oceans Toxic Chemical Pollutants in the Oceans Have Been Shown Capable of Causing a Wide Range of Human Diseases
Supplementary Appendix to “Human Health and Ocean Pollution”, Annals of Global Health, 2020 This Supplementary Appendix contains additional references and documentation supporting the information presented in the report, Human Health and Ocean Pollution. Chemical Pollution of the Oceans Toxic chemical pollutants in the oceans have been shown capable of causing a wide range of human diseases. Toxicological and epidemiological studies document that pollutants such as toxic metals, POPs, dioxins, plastics chemicals, and pesticides can cause cardiovascular effects, developmental and neurobehavioral disorders, metabolic disease, endocrine disruption and cancer. Table 1 in this Supplementary Appendix summarizes the known links between chemical pollutants in the oceans and a range of human health outcomes. The strengths of the associations listed in Table 1 vary depending on the nature of the studies establishing these associations. Some associations have been assessed in systematic reviews and meta-analyses of animal and human data.1 2 Some are single cross-sectional or case-control studies. There are now a growing number of relevant epidemiological studies, including powerful prospective cohort studies, such as the Nurses’ Health Study II and the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS)3 Findings from these investigations are strengthening the evidence base for associations between exposures to organic chemical pollutants and adverse health outcomes. Supplementary Appendix Table 1. Adverse Human Health Outcomes -
Oceanological and Hydrobiological Studies
Oceanological and Hydrobiological Studies International Journal of Oceanography and Hydrobiology Volume 49, No. 1, March 2020 ISSN 1730-413X pages (34-48) eISSN 1897-3191 Dominant species drive seasonal dynamics of the sh community in the Min estuary, China by Abstract Fishery resources are currently facing multiple stresses Jun Li, Bin Kang* such as over shing, pollution and climate change. Looking into processes and mechanisms of the dynamic sh community through detailed quantitative analyses contributes to e ective conservation and management of shery resources. The Min estuary plays an important role in maintaining sheries in southeastern coastal China, therefore the sh community in the brackish area was investigated and analyzed in this study. A total of 127 DOI: 10.1515/ohs-2020-0004 species belonging to 91 genera, 49 families and 14 orders Category: Original research paper were sampled in 2015. Eight indices re ecting four aspects of sh communities were determined, i.e. species richness, Received: May 5, 2019 species evenness, heterogeneity and taxonomy. Di erences Accepted: August 21, 2019 between the indices were nonsigni cant, suggesting that the use of a single diversity descriptor could not provide a full explanation. Nine dominant species in the Min estuary showed seasonal turnover by rational use of resources and Jimei University, 185 Yinjiang Rd., co-occurring species showed correspondingly adequate 361021 Xiamen, China habitat preferences and feeding habits to avoid competition. The species Harpadon nehereus occurred as the dominant species in three seasons except spring. High values of niche overlap among common or rare species and lower values of niche overlap among all dominant species e ectively brought the diversity of the sh community into a state of equilibrium. -
Common Diseases of Wild and Cultured Fishes in Alaska
COMMON DISEASES OF WILD AND CULTURED FISHES IN ALASKA Theodore Meyers, Tamara Burton, Collette Bentz and Norman Starkey July 2008 Alaska Department of Fish and Game Fish Pathology Laboratories The Alaska Department of Fish and Game printed this publication at a cost of $12.03 in Anchorage, Alaska, USA. 3 About This Booklet This booklet is a product of the Ichthyophonus Diagnostics, Educational and Outreach Program which was initiated and funded by the Yukon River Panel’s Restoration and Enhancement fund and facilitated by the Yukon River Drainage Fisheries Association in conjunction with the Alaska Department of Fish and Game. The original impetus driving the production of this booklet was from a concern that Yukon River fishers were discarding Canadian-origin Chinook salmon believed to be infected by Ichthyophonus. It was decided to develop an educational program that included the creation of a booklet containing photographs and descriptions of frequently encountered parasites within Yukon River fish. This booklet is to serve as a brief illustrated guide that lists many of the common parasitic, infectious, and noninfectious diseases of wild and cultured fish encountered in Alaska. The content is directed towards lay users, as well as fish culturists at aquaculture facilities and field biologists and is not a comprehensive treatise nor should it be considered a scientific document. Interested users of this guide are directed to the listed fish disease references for additional information. Information contained within this booklet is published from the laboratory records of the Alaska Department of Fish and Game, Fish Pathology Section that has regulatory oversight of finfish health in the State of Alaska. -
Poros Filodiales En La Identificación De Dos Subespecies De Erizos De Mar: Meoma Ventricosa Grandis (Pacífico) Y Meoma Ventricosa Ventricosa (Atlántico) En México
Poros filodiales en la identificación de dos subespecies de erizos de mar: Meoma ventricosa grandis (Pacífico) y Meoma ventricosa ventricosa (Atlántico) en México M.A. Torres-Martínez1, F.A. Solís-Marín2, A. Laguarda-Figueras2 & B.E. Buitrón Sánchez3 1. Posgrado en Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México (UNAM), México D.F., México;[email protected] 2. Laboratorio de Sistemática y Ecología de Equinodermos, Instituto de Ciencias del Mar y Limnología (ICML), UNAM, Apdo. post. 70-305, México D.F. 04510, México; [email protected] 3. Instituto de Geología, Departamento de Paleontología, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Delegación Coyoacán, 04510, México D.F., México; [email protected] Recibido 23-VIII-2007. Corregido 23-IV-2008. Aceptado 17-IX-2008. Abstract: Phyllodial pores and the identification of two subspecies of sea urchins in Mexico: Meoma ventri- cosa grandis (Pacific) and Meoma ventricosa ventricosa (Atlantic). The genus Meoma inhabits Mexican waters and is represented by the subspecies Meoma ventricosa grandis in the Pacific and Meoma ventricosa ventricosa in the Atlantic. Both subespecies are morphologically similar. We studied the morphological differences between Meoma ventricosa grandis and Meoma ventricosa ventricosa, specifically in the patterns of phyllodial pore pairs and kind of sediments where they live. The number of pores differs among subspecies until M. ventricosa grandis reaches 110 mm of total lenght. The difference in the number of phyllodial pores can be an adaptation to the size of silt grain. Rev. Biol. Trop. 56 (Suppl. 3): 13-17. Epub 2009 January 05. Key words: sea urchins, Meoma ventricosa grandis, Meoma ventricosa ventricosa, México, phyllodial pores. -
NASCO Scientific Working Group
N A S C O NORTH AMERICAN COMMISSION PROTOCOLS FOR THE INTRODUCTION AND TRANSFER OF SALMONIDS by NAC/NASCO Scientific Working Group on Salmonid Introductions and Transfers Edited by T. Rex Porter Canadian Co-chairman Department of Fisheries and Oceans P O Box 5667 St John's, NF A1C 5X1 NAC(92)24 i TABLE OF CONTENTS Page INTRODUCTION ............................................................................................................... 1 PART I SUMMARY OF PROTOCOLS BY ZONE .............................................. 3 1 ZONING OF RIVER SYSTEMS ......................................................................... 5 2 DESCRIPTION OF ZONES ................................................................................. 5 3 PROTOCOLS ......................................................................................................... 6 3.1 Protocols applicable to all three Zones ..................................................... 6 3.2 Protocols applicable to Zone I ................................................................... 7 3.2.1 General within Zone I ................................................................................... 7 3.2.2 Rehabilitation ................................................................................................ 7 3.2.3 Establishment or re-establishment of Atlantic salmon in a river or part of a watershed where there are no salmon ........................................ 7 3.2.4 Aquaculture .................................................................................................. -
Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341 -
Scholars Academic Journal of Biosciences
Scholars Academic Journal of Biosciences Abbreviated Key Title: Sch Acad J Biosci ISSN 2347-9515 (Print) | ISSN 2321-6883 (Online) Zoology Journal homepage: https://saspublishers.com A Comprehensive Review on the Prevalence and Dissemination of Some Bacterial Diseases in Ornamental Fishes and Their Preventive Measures Arnab Chatterjee1#, Sucharita Ghosh2#, Ritwick Bhattacharya1#, Soumendranath Chatterjee2, Nimai Chandra Saha1* 1Fishery and Ecotoxicology Research Laboratory (Vice-Chancellor’s Research Group), Department of Zoology, the University of Burdwan, Burdwan 713104, West Bengal, India 2Parasitology & Microbiology Research Laboratory, Department of Zoology, the University of Burdwan, Burdwan, West Bengal, India #Authors contributed equally DOI: 10.36347/sajb.2020.v08i11.005 | Received: 06.11.2020 | Accepted: 17.11.2020 | Published: 20.11.2020 *Corresponding author: Nimai Chandra Saha Abstract Review Article As a consequential sector within the fisheries segment, ornamental fisheries have become a billion-dollar industry. At current, it is estimated that the aquarium industry is worth about 15 billion dollars. In ornamental aquaculture and aquarium keeping, the incidence of diseases is the main quandary that emerges during culture and deplorably affects the profitability of the ventures. Diseases are caused by viruses, protozoa, bacteria, fungi, and parasites under profound culture conditions, and the likelihood of stress elevates in an immensely colossal portion of the stock. Of these, the most paramount causes of sudden fish death are infectious and bacterial diseases. Nowadays, veterinary antibiotic treatment of contaminated fish is being applied in most of the States of India. Disease obviation is often less costly than treating disease outbreaks when it is subsisting. Adopting and implementing a health management strategy would not assure a disease-free facility that ultimately leads to considerably decremented chances of dissemination of diseases. -
Dissodactylus Crinitichelismoreira, 1901 and Leodia Sexiesperforata
Nauplius 19(1): 63-70, 2011 63 Dissodactylus crinitichelis Moreira, 1901 and Leodia sexiesperforata (Leske, 1778): first record of this symbiosis in Brazil Vinicius Queiroz, Licia Sales, Elizabeth Neves and Rodrigo Johnsson LABIMAR (Crustacea, Cnidaria & Fauna Associada), Universidade Federal da Bahia. Avenida Adhemar de Barros s/nº, Campus Ondina. CEP 40170- 290. Salvador, BA, Brazil. E-mail: (VQ) [email protected]; (LS) [email protected]; (EN) [email protected]; (RJ) [email protected] Abstract The crabs of the genusDissodactylus are well known as ectosymbionts of irregular echinoids belonging to Clypeasteroida and Spatangoida. Dissodactylus crinitichelis is the only species of the genus reported in Brazil. The pea crab species has been already recorded associated with four species of echinoids in Brazilian waters. This paper reviews the known hosts for D. crinitichelis and registers for the first time the association between the pea crab and the sand dollar Leodia sexiesperforata increasing to five the number of known hosts for the crab. Key Words: Ecological association, ectosymbiont, Pinnotheridae. Introduction includes about 302 species of little crabs (Ng et al., 2008) highly specialized in living The diversity of the marine environment, in close association with other invertebrates. specially the benthic substratum is commonly The family is known for their association reflected by many interactions among with various invertebrate taxa, such as organisms, even free living ones. Such event molluscs, polychaetes, ascidians, crustaceans is quite common since many of these species or echinoderms (holothurians and irregular act as substratum or environment for others. echinoids) (Schmitt et al., 1973; Powers, 1977; The existence of many organisms living in Williams, 1984; Takeda et al., 1997; Thoma association and their close relation allows for et al., 2005, 2009; Ahyong and Ng, 2007).