Biologia 72/7: 796—806, 2017 Section Zoology DOI: 10.1515/biolog-2017-0087
DNA barcoding and first records of two rare Adicella species (Trichoptera: Leptoceridae) in Croatia
Anđela Cukuši´ c´1,RenataCuk´ 2,AnaPrevišic´3,MartinaPodnar4,AntunDelic´5 &MladenKučinic´3*
1Geonatura Ltd. Consultancy in Nature Protection, Fallerovo šetalište 22, 10000 Zagreb, Croatia 2Hrvatske vode, Central Water Management Laboratory, Ulica grada Vukovara 220, 10000 Zagreb, Croatia 3Department of Biology, Laboratory for Entomology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia; e-mail: [email protected] 4Croatian Natural History Museum, Demetrova 1, 10 000 Zagreb, Croatia 5Faculty of Ecudation, Department in Petrinja, University of Zagreb, Matice Hrvatske 12, 44250, Petrinja, Croatia
Abstract: Two species of the genus Adicella, A. cremisa Malicky, 1972 and A. balcanica Botosaneanu & Novak, 1965, were recorded in the summer period of 2014 which represent the first records of these species in Croatia. The former was collected at two relatively distant sites, the Krapina and the Zrmanja rivers, while the later was collected at the Krupa River. Both species were identified by morphological characteristics as well as DNA barcoding method. We calculated the uncorrected pairwise distances within Adicella and used molecular phylogenetic approach to delimit species.A.cremisafrom Croatia showed no significant difference in the mtCOI region and they are highly similar to A. cremisa from Italy. Additionally, the ecological preferences and distribution of Adicella species are presented. Our findings represent a significant contribution to the aquatic biodiversity of the Western Balkans. Key words: molecular identification; aquatic insects; caddisfly
Introduction The Croatian caddisfly fauna was encompassed in several papers using the mtCOI gene in similar con- DNA barcoding method is used to identify species of text (e.g., Previši´c et al. 2009, 2014; Kučini´cetal. different groups of organisms (animals, plants, fungi) 2010), however, not the “barcode region” (mtDNA and is based on sequencing of the standardized segment COI-5P). The barcode region was used only to sup- of the mitochondrial (mt) cytochrome c oxidase subunit port the description of the new species Chaetopteryx 1 (COI) gene (Hebert et al. 2003). Advantages of us- bucari Kučini´c, Szivak & Deli´c, 2013 (Kučini´cetal. ing DNA barcodes in species identification compared 2013) and the new record of Tinodes antonioi Boto- to other parts of the genome are the lack of introns saneanu & Taticchi-Vigan`o, 1974 (Kučini´c et al. 2016). (unlike in nuclear genes), simple sequence alignment This paper presents the first finding of the two (compared to mitochondrial ribosomal genes, e.g., 12S, caddisfly species from the genus Adicella: A. cremisa 16S), a great range of phylogenetic signal, and widely Malicky, 1972 and A. balcanica Botosaneanu & No- used robust primers able to successfully amplificate the vak, 1965 in Croatia. The genus Adicella MacLachlan, barcode region in variety of taxa (Hebert et al. 2003). 1877 belongs to the tribe Triaenodini Morse, 1981 of DNA barcoding of caddisflies has overall wide ap- the long-horned caddisfly family Leptoceridae. Family plication in association of previously unknown larval Leptoceridae has a cosmopolitan distribution and it is stages with adults (e.g., Zhou et al. 2007; Zhou 2009; the second largest family of caddisflies in the world with Graf et al. 2009; Ruiter et al. 2013; Gill et al. 2014). a total of 1567 described species (Morse 2003; Holzen- Similarly, in European caddisflies, the barcode region thal & Pes 2004). This family has two subfamilies, the was used in studies mainly to support description of subfamily Triplectidinae distributed in southern hemi- new species and to associate previously unknown lar- sphere and the more cosmopolitan subfamily Leptoceri- val stages (e.g., Gíslason et al. 2015; Graf et al. 2015; nae, containing 14 and 30 genera, respectively (Morse Waringer et al. 2015; Vitecek et al. 2015b) and to per- & Holzenthal 1987; Holzenthal & Pes 2004). form phylogenetic analyses (Kučini´c et al 2013; Vitecek The genus Adicella is distributed in the West and et al. 2015a, 2017). East Palaearctic biogeographic region, the Afrotropical
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