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BioMed Research International Environmental Biotechnology: Current Advances, New Knowledge Gaps, and Emerging Issues Guest Editors: Abd El-Latif Hesham, T. Komang Ralebitso-Senior, Yu Zhang, and Qing X. Li Environmental Biotechnology: Current Advances, New Knowledge Gaps, and Emerging Issues BioMed Research International Environmental Biotechnology: Current Advances, New Knowledge Gaps, and Emerging Issues Guest Editors: Abd El-Latif Hesham, T. Komang Ralebitso-Senior, Yu Zhang, and Qing X. Li Copyright © 2015 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “BioMed Research International.” All articles are open access articles distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Contents Environmental Biotechnology: Current Advances, New Knowledge Gaps, and Emerging Issues, Abd El-Latif Hesham, T. Komang Ralebitso-Senior, Yu Zhang, and Qing X. Li Volume 2015, Article ID 814529, 2 pages Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and Antimicrobial Resistance, Raquel Soares Casaes Nunes, Eduardo Mere Del Aguila, and Vânia Margaret Flosi Paschoalin Volume 2015, Article ID 483548, 17 pages Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process, Jeong Heo Kwon, Siseon Lee, Jae-Won Lee, Youn-Woo Hong, Jeong Ho Chang, Daekyung Sung, Sung Hyun Kim, Byoung-In Sang, Robert J. Mitchell, and Jin Hyung Lee Volume 2015, Article ID 267538, 5 pages Fabrication of an Amperometric Flow-Injection Microfluidic Biosensor Based on Laccase for In Situ Determination of Phenolic Compounds,JuanC.Gonzalez-RiveraandJohannF.Osma Volume 2015, Article ID 845261, 9 pages Comparison of Different Strategies for Selection/Adaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel,C.Varrone,T.M.B.Heggeset, S. B. Le, T. Haugen, S. Markussen, I. V. Skiadas, and H. N. Gavala Volume 2015, Article ID 932934, 14 pages Perspective for Aquaponic Systems: “Omic” Technologies for Microbial Community Analysis, Perla Munguia-Fragozo, Oscar Alatorre-Jacome, Enrique Rico-Garcia, Irineo Torres-Pacheco, Andres Cruz-Hernandez, Rosalia V. Ocampo-Velazquez, Juan F. Garcia-Trejo, and Ramon G. Guevara-Gonzalez Volume 2015, Article ID 480386, 10 pages Magnet-Facilitated Selection of Electrogenic Bacteria from Marine Sediment, Larisa Kiseleva, Justina Briliute, Irina V. Khilyas, David J. W. Simpson, Viacheslav Fedorovich, M. Cohen, and Igor Goryanin Volume2015,ArticleID582471,7pages Fungal Antagonism Assessment of Predatory Species and Producers Metabolites and Their Effectiveness on Haemonchus contortus Infective Larvae, Manoel Eduardo Silva, Fabio Ribeiro Braga, Pedro Mendoza de Gives, Jair Millán-Orozco, Miguel Angel Mercado Uriostegui, Liliana Aguilar Marcelino, Filippe Elias de Freitas Soares, Andréia Luiza Araújo, Thainá Souza Vargas, Anderson Rocha Aguiar, Thiago Senna, Maria Gorete Rodrigues, Frederico Vieira Froes, and Jackson Victor de Araújo Volume 2015, Article ID 241582, 6 pages Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae, Manoel Eduardo da Silva, Fabio Ribeiro Braga, Pedro Mendoza de Gives, Miguel Angel Mercado Uriostegui, Manuela Reyes, Filippe Elias de Freitas Soares, Lorendane Millena de Carvalho, Francielle Bosi Rodrigues, and Jackson Victor de Araújo Volume 2015, Article ID 474879, 5 pages Biosorption of Pb(II) Ions by Klebsiella sp. 3S1 Isolated from a Wastewater Treatment Plant: Kinetics and Mechanisms Studies, Antonio Jesús Muñoz, Francisco Espínola, Manuel Moya, and Encarnación Ruiz Volume 2015, Article ID 719060, 12 pages Hindawi Publishing Corporation BioMed Research International Volume 2015, Article ID 814529, 2 pages http://dx.doi.org/10.1155/2015/814529 Editorial Environmental Biotechnology: Current Advances, New Knowledge Gaps, and Emerging Issues Abd El-Latif Hesham,1 T. Komang Ralebitso-Senior,2 Yu Zhang,3 and Qing X. Li4 1 Genetics Department, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt 2School of Science and Engineering, Teesside University, Borough Road, Middlesbrough, Tees Valley TS1 3BA, UK 3Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China 4Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA Correspondence should be addressed to Abd El-Latif Hesham; hesham [email protected] Received 19 November 2015; Accepted 19 November 2015 Copyright © 2015 Abd El-Latif Hesham et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The term “environmental biotechnology” encapsulates a wide gene expression, protein translation, and, more recently, the and dynamic spectrum of topics including bioremediation, metabolite network, respectively. They indicated essential biofiltration, wastewater treatment, biodegradation, waste roles of “omic” approaches such as metagenomics and meta- management, and biofuel production. All these disciplines transcriptomics on future studies of microbial diversity in are underpinned by complex interacting microbial commu- aquaponic biosystems. nities; hence exciting research has been undertaken and C. Varrone et al. reported their research on the selection increasingly driven by cutting-edge ecogenomic techniques. and adaptation of mixed microbial cultures (MMCs), able However, understanding of microbial diversity, population, to ferment crude glycerol generated from animal fat-based and structure for sustainable environmental management biodiesel and produce building-blocks and green chemicals. remains largely a “black box.” Thus exciting findings identi- Various adaptation strategies have been investigated for the fied new knowledge gaps and, inevitably, engendered novel enrichment of suitable and stable MMCs, trying to over- research questions with inventive methodologies. come inhibition problems and enhance substrate degradation Thisspecialissueisfocusedonoriginalresearchand efficiency, as well as production of soluble fermentation review articles that (i) present a critical and succinct appre- products. Repeated transfers in small batches and fed-batch ciation of the environmental biotechnology state of the art, conditions were applied. They demonstrated that next gen- (ii) identify emerging areas of research, (iii) illustrate the eration sequencing represented a useful tool to monitor the applications of innovative molecular techniques, and (iv) changes in microbial composition of MMCs, highlighting suggest updates of established protocols. the development of a glycerol consuming community (with P. Munguia-Fragozo and his colleagues have systemati- numerous strains belonging to the genera Clostridium, Kleb- cally reviewed the omic technologies for microbial commu- siella,andEscherichia), thus confirming the effectiveness of nity analysis. They discuss the potential applications of cur- the enrichment strategy. rent “omics” such as denaturing gradient gel electrophoresis, L. Kiseleva et al. have focused their work on the isolation microscopy using fluorescence in situ hybridization, and/or and partial characterization of an electrogenic bacterium cloning rRNA gene fragments and bioinformatic tools to Thalassospira sp. strain HJ from a magnetic particle-enriched characterize the microbial community of aquaponic systems. portion of a marine tidal sediment. They described the Also they mentioned that metatranscriptomics, proteomics, first report of electrogenic behavior within the genus Tha- and metabolomics can provide information of functional lassospira. They recommended that a more extensive study analyses in microbial communities at different levels of would be needed to determine whether the proportion of 2 BioMed Research International electrogenic bacteria obtained from this magnetic particle CNS strains were identified by sequencing of a 16S rDNA enrichment procedure exceeds that found in the environ- region and the phylogenic relationships between the enriched ment. With their diurnal patterns of flooding and diversity species were established. The presence of multiple genes of mineral components, tidal sediments should be rich encoding the classical and newly described se/sel and tstH1 environments to bioprospect for electrogenic bacteria. toxins in the CNS genomes was investigated. The risk of food Developmentofanin situ microfluidic biosensor for poisoning was then assessed by evaluating the ability of the the detection of phenol, based on laccase from Trametes CNS strains in transcribing and expressing the classical and pubescens with flow-injection and amperometry as the trans- newly described enterotoxins in vitro by using real-time PCR ducer method, is reported by J. C. Gonzalez-Rivera and J. and enzyme-linked immunosorbent assays. The resistance F. Osma in their study of “Fabrication of an Amperometric of the isolated strains to antimicrobial agents of therapeutic Flow-Injection Microfluidic Biosensor Based on Laccase importance in staphylococci infections was also evaluated. for In Situ Determination of Phenolic Compounds.” The Finally, J. H. Kwon et al. described the optimization of microfluidic biosensor