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Phylogenetics of Cladopyxidoiddinophytes

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Phylogenetics of Cladopyxidoiddinophytes Fensomea setacea, gen. & sp. nov. (Cladopyxidaceae, Dinophyceae), is neither gonyaulacoid nor peridinioid as inferred from morphological and molecular data Marc Gottschling, Maria Consuelo Carbonell-Moore, Kenneth Neil Mertens, Monika Kirsch, Malte Elbrächter, Urban Tillmann Supplementary Material Figure S1: Friedrich v. Stein’s original material of Cladopyxis brachiolata (reproduction of pl. II 7–13). Note that pl. II 7 is chosen as lectotype. Furthermore, pl. II 13 is likely gonyaulacean †Spiniferites mirabilis (M.Rossignol) Matsuoka and pl. II 12 possibly also a species of †Spiniferites Mantell. Figure S2: Variability in plate pattern and arrangement of Fensomea setacea, gen & sp. nov., (GeoB*184) regarding (A–E) epithecal plates and (F–I) hypothecal plates. (A) Presence of only six precingular plates. (B) Loss of one epithecal plate interpreted here as a fusion of plates 3' and 4a. (C) Basic plate pattern, but unusually plates 2' and 3' are of equal size and in lateral position. (D) Loss of one epithecal plate interpreted here as fusion of apical plates 2' and 3'. (E) Presence of one additional epithecal plate interpreted here as a subdivison of plate 3'. (F) Basic plate pattern, but unusually plates 1'''' and 2'''' are of equal size and symmetrically arranged. Note that the list of plate 1''' towards plate Sp is missing. (G) Loss of two hypothecal plates, only 5 precingular plates and one antapical plate present. (H–I) Presence of three antapical plates, interpreted here as a subdivision of plate 2''''. Scale bars = 5 µm. Tables Table S1: Voucher list. All names are given under the rules of the ICN, the author standard forms follow (Brummitt and Powell 1992). Abbreviation: n. inf., no information. If ‘holotype’ or ‘epitype’ is noted for a species name, then it refers to material, from which the type was prepared. Species name with author Strain No. Locality Date Collector(s) GenBankNo(s) Reference outgroup Amoebophrya sp. [isolated n.inf. western North Atlantic, n.inf. n.inf. HM483394 Coats et al. (2010) from Levanderina fissa off USA–MD: Chesapeake (SSU+ITS+LSU) (Levander) Moestrup, Bay Hakanen, Gert Hansen, Daugbjerg & M.Ellegaard] Amoebophrya sp. [isolated n.inf. western North Atlantic, n.inf. n.inf. HM483395 Coats et al. (2010) from Akashiwo sanguinea off USA–MD: Chesapeake (SSU+ITS+LSU) (K.Hirasaka) Gert Hansen & Bay Moestrup] Besnoitia besnoiti (E.E.Franco Israel n.inf. n.inf. DQ227420 (rRNA) Cortes et al. (unpubl. & I.Borges, 1916) 2005) Euduboscquella sp. [isolated OC20 USA–MD: Ocean City, Aug n.inf. JN934989 Bachvaroff et al. (2012) from Favella arcuata Assawoman Bay (38°20'N, 11, (SSU+ITS+LSU) (K.Brandt, 1906)] 75°06W) 2010 marine alveolate FBB25 USA–MA: Boston, Blanes n.inf. n.inf. EU304548 (rRNA) Massana et al. (2008) Bay Microbial Observatory Perkinsus andrewsi Coss, ATCC50807 (≡ USA–MD: Rhode River n.inf. n.inf. AY305326 (rRNA) Pecher et al. (2004) J.Robledo, G.Ruiz & Vasta, PAND-A8–4a) 2001 [isolated from Macoma balthica (Linnaeus, 1758)] Perkinsus atlanticus ALG1 Portugal n.inf. n.inf. AF509333 (rRNA) Robledo et al. (2002) C.Azevedo, 1989 (isolated from Venerupis decussata Linnaeus, 1758) Toxoplasma gondii (Nicolle & P n.inf. n.inf. n.inf. X75453 Ding et al. (unpubl. Manceaux, 1908) (isolated (SSU+ITS+LSU) 1993) from Homo sapiens Linnaeus, 1758) Vitrella brassicaformis NCMA3155 (≡ off Australia: Great Barrier Jan 12, K. Miller & C. Mundy HM245049 (rRNA) Janouškovec et al. Oborník, D.Modrý, M.Lukeš, RM11) Reef (23°30'S, 152°00'E) 2001 [R.A. Andersen & R. (2010) Cernotíková-Stříbrná, Cihlář, Moore] s.n. Tesařová, Kotabová, Vancová, Prášil & J.Lukeš (isolated from an anthozoan species) unplaced dinophytes Akashiwo sanguinea GnSg02 western North Pacific, n.inf. M. Chang s.n. AY831410 (rRNA) Ki and Han (2005) (K.Hirasaka) Gert Hansen & East China Sea, off South Moestrup Korea: Jangmok Akashiwo sanguinea NCMA1837 (≡ western North Atlantic, Sep 23, S. Sarkis s.n. DQ779988 (rRNA) Ki and Han (2007a) (K.Hirasaka) Gert Hansen & CCCM885) Sargasso Sea, off UK, the 1997 Moestrup Bermudas: Harington Sound off Rabbitt Island (32°20'N, 64°44'W) Amphidinium carterae Hulburt NCMA124 (≡ eastern North Pacific, off Jun 20, R. Norris s.n. AF274255 (SSU), Saldarriaga Echavarría et CCCM439, Mexico: Sonora, Puerto 1966 EU927575 (ITS), al. (2001), Murray et al. UW380) Penasco (31°19'N, AY460584 (LSU) (2004) Ferrell & Beaton 113°34'W) (unpubl. 2008) Amphidoma languida 2A11 North Atlantic, off Iceland Aug, U. Tillmann [Maria KR362880 (SSU), Tillmann et al. (2015) Tillmann, R.Salas & Elbr. (65°27'N, 24°39'W) 2012 S. Merian] [U. KR362882 (ITS), Tillmann] s.n. KR362885 (LSU) Ankistrodinium armigerum n.inf. western North Pacific, Sea n.inf. n.inf. AB858349 (SSU), Watanabe et al. (2014) K.Watanabe, Miyoshi, F.Kubo, of Japan, off Japan: AB858350 (LSU) Sh.Murray & T.Horig. Hokkaidō, Ishikari Beach (43°15'N, 141°21'E) Apicoporus glaber n.inf. (chimeric) Germany: Sylt Mar, n.inf. EU293235 (SSU), Sparmann et al. (2008), (Hoppenrath & Okolodkov) 2009 JQ179867 (LSU) Hoppenrath et al. (2012) Sparmann, B.S.Leander & Hoppenrath Azadinium caudatum var. IFR1191 [IFR10- eastern North Atlantic, Dec [E. Nézan] s.n. JQ247701 (SSU), Nézan et al. (2012) caudatum (Halldal) Nézan et 332, IFR10-330, Celtic Sea, off France: 15, JQ247700 (ITS), Chomérat IFR11-033] Brittany, Finistère, 2009 JQ247702 (LSU) Concarneau (47°50'N, 3°57'W) Azadinium concinnum 1C6 (single and North Atlantic, Irminger Aug, U. Tillmann [Maria KJ481826 (SSU), Tillmann et al. (2014) Tillmann & Nézan (holotype) multiple cells, LSU Sea, off Greenland 2012 S. Merian] [U. KJ481827 (ITS), clone 4) (62°14'N, 37°27'W) Tillmann] s.n. KJ481831 (LSU) Azadinium poporum Tillmann TIO256 Mediterranean, off Jan 18, n.inf. MF033112 (SSU), Luo et al. (2017) & Elbr. France: Corsica (42°08'N, 2016 MF033116 (ITS), 9°32'E) MF033123 (LSU) Azadinium spinosum Elbr. & SHETF6 North Sea, off Shetland May, U. Tillmann JX559885 Tillmann et al. (2012) Tillmann Islands (60°13'N, 1°00'W) 2011 [Heincke] [U. (SSU+ITS+LSU) Tillmann] s.n. Bernardinium sp. CCP2 USA–NC: Pond near Jul, n.inf. JQ439940 (rRNA) Fawcett and Parrow Cashiers (35°08'N, 2009 (2012) 83°05'W) Bispinodinium angelaceum HG236 western North Pacific, off May R. Terada s.n. AB762397 (SSU), Yamada et al. (2013) N.Yam. & T.Horig. (holotype) Japan: Kyūshū, 15, AB762398 (LSU) Kagoshima, off 2008 Mageshima (30°41' N, 130°50'E) Brachidiniaceae sp. GrAr01 western North Pacific, Sea n.inf. n.inf. DQ779991 (rRNA) Ki and Han (2007a) of Japan, off South Korea: Chilchondo Bysmatrum subsalsum KC32CCAUTH off Greece, North Aegean n.nf. N. Nikolaidis s.n. HQ845326 Gottschling et al. (2012) (Ostenf.) M.A.Faust & Steid. Sea: Thessaloniki, Porto- (SSU+ITS+LSU) Lagos (40°58'N, 25°07'E) Ceratoperidinium falcatum IFR1100 eastern North Atlantic, Oct 5, REPHY KJ508394 (LSU) Nézan et al. (2014) (Kof. & Swezy) A.Reñé & de Celtic Sea, off France: 2009 Salas Brittany, Finistère, Douarnenez (48°10'N, 4°24'W) Fensomea setacea Tillmann & GeoB*184 western South Atlantic Mar 7, K.J.S. Meier & M. MW267274 this study Gottschling (holotype) (31°25'S, 37°31'W, ‒5m) 2000 Streng [Meteor (SSU+ITS+LSU), 46/4] [M. Kirsch] WP MW267282 3/7/a (LSUd8d10) Gertia stigmatica K.Takahashi, mdd472-kt western North Pacific, off Oct 20, K. Takahashi s.n. LC490696 (rRNA) Takahashi et al. (in G.Benico, Wai Mun Lum & Japan, Sagami Bay: 2016 press) M.Iwataki Honshū, Kantō, Kanagawa, Manazuru (35°09'N, 139°10'E) Glenodiniopsis steinii Wołosz. NIES463 (≡ Japan: Iwate, Shizukuishi Sep 10, [T. Sawaguchi] s.n. AF274257 (SSU), Saldarriaga Echavarría et (reference material) TM3D6) 1984 EF058255 (LSU) al. (2001), Logares et al. (2007b) “Gymnodinium” impatiens CCAC0025 (≡ Germany: Brandenburg, 1992 [B. Marin] s.n. EF058239 (SSU), Logares et al. (2007b) Skuja M0925) Neuglobsow EF058259 (LSU) Gyrodinium dominans Hulburt GDMS0704YD western North Pacific, Apr 1, Y.D. Yoo s.n. FN669510 Yoon et al. (2012) East China Sea, off South 2007 (SSU+ITS+LSU) Korea: Gyeongsangnam- do, Masan Bay Gyrodinium helveticum K1 Russia, Lake Baikal: 2012 n.inf. MG493227 (SSU), Annenkova (2018) (Penard) Y.Takano & T.Horig. Irkutsk, between MG255302 (reference material) Listvyanka and Bolshie (ITS+LSU) Koty Hemidinium nasutum F.Stein NIES471 (≡ Japan: Honshū, Ibaraki, Aug [T. Sawaguchi s.n.] AY443016 (SSU), Saldarriaga Echavarría et (reference material) 87SPD1) Tsuchiura 27, EF058260 (LSU) al. (2004), Logares et al. 1987 (2007b) Jadwigia applanata Moestrup, CCAC0021 (≡ Germany: Hessen, 1991 [A. Schilke] s.n. EF058240 (SSU), Lindberg et al. (2005), K.Lindb. & Daugbjerg SCCAP K-1115) Biebergemünd, near AY950447 (LSU) Logares et al. (2007b) (holotype) Lochmühle Karenia mikimotoi (Miyake & NCMA429 (≡ UK: England, Devon, Aug D. Harbour s.n. FJ587220 (SSU), Hansen et al. (2000), Komin. ex M.Oda) Gert CCAP1127/1, G1, Plymouth, Sutton Harbour 23, HM807318 (ITS), Garces et al. (2006), Hou Hansen & Moestrup PLY497a) (50°22'N, 4°10'W) 1980 AF200678 (LSU) et al. (unpubl. 2008), Stern et al. (2010), Al- Kandari et al. (2011) Kirithra asteri Boutrup, H-1-A6 western South Atlantic, Sep 9, U. Tillmann MW267281 (SSU), Boutrup et al. (2017), Tillmann, Daugbjerg & off Argentina (41°11'S, 2015 [Houssay] [U. MW267275 (ITS), this study Moestrup 57°52'W, ‒5m) Tillmann] s.n. MF666674 (LSU) Levanderina fissa (Levander) NCMA431 (≡ Portugal: near Santiago do Nov 1, E. Silva s.n. AY443015 (SSU), Saldarriaga Echavarría et Moestrup, Hakanen, Gert CCCM796, Cacem, Santo Andre 1980 JQ972685 (ITS), al. (2004), Moestrup and Hansen, Daugbjerg & LISBOA176) lagoon (38°03'N, 8°48'W) EF205007 (LSU) Daugbjerg (2007), Stern M.Ellegaard et al. (2012) Moestrupia sp. AW22-12 western North Pacific, off Apr 22, S. Suda s.n. LC025890 (SSU), Prabowo et al. (2016) Japan: Okinawa, Awase 2013 LC025909 (ITS), (26°19'N, 127°50'E) LC025928 (LSU) Moestrupia sp. AW22-15 western North Pacific, off Apr 22, S.
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