Seasonal Changes in the Mya Arenaria (L.) Population from Inner Puck Bay 179
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Seasonal changes in OCEANOLOGIA, 39 (2), 1997. pp. 177–195. the Mya arenaria (L.) 1997, by Institute of population from Inner Oceanology PAS. Puck Bay KEYWORDS Mya arenaria Population dynamics Biochemical composition Inner Puck Bay Justyna Miąc, Małgorzata Groth, Maciej Wołowicz Institute of Oceanography, Gdańsk University, Gdynia Manuscript received April 4, 1997, in final form May 20, 1997. Abstract Morphometric relationships, the growth rate, sex distribution and annual fluctua- tion in dry flesh weight, biochemical composition and energy value of the sublittoral bivalve Mya arenaria (L.) from Inner Puck Bay were studied from May 1994 to April 1995. M. arenaria grows rapidly during the first year of its life, after which the growth rate decreases. The oldest individual was 5 years old and 53 mm long. Judging from the sex distribution and analysis of biochemical composition, the soft-shell clam breeds in June–July. Analysis of the growth rate and the age distribution confirms the theories that specimens of M. arenaria found in low latitudes reach a smaller maximum size and grow more slowly than those living in higher latitudes. The biochemical composition was determined in the 20–30 mm length class. The mean percentages of the main components of dry flesh were: proteins 47.54 ± 6.0 (male), 51.28 ± 7.7 (female); carbohydrates 9.28 ± 3.7 (male), 10.40 ± 4.0 (female); glycogen 5.74 ± 2.8 (male), 6.72 ± 3.5 (female); lipids 8.67 ± 1.8 (male), 8.79 ± 1.3 (female). Analysis of lipid and carbohydrate (glycogen) content in tissues of the soft-shell clam yielded the highest values in the residue (gonads and hepatopancreas). The lipid level is much higher in early spring, before spawning, than in autumn, when gonad development begins. The highest energy values are reported for August (20.82 J mg−1 in both sexes), the lowest for October (14.38 J mg−1 in males and 14.98 J mg−1 in females). 178 J. Miąc, M. Groth, M. Wołowicz Seasonal changes in energy values are mainly connected with the availability of food and reproduction cycle. 1. Introduction Even though the soft-shell clam, Mya arenaria, is a very common species in Inner Puck Bay, its growth and population dynamics have only been occasionally investigated. Information about this species can be found in Mulicki (1957), Żmudziński (1967), Wenne and Wiktor (1982) and Żmudziński (1990). It must be pointed out that none of these investigations concentrated on M. arenaria; their purpose was to provide a brief, general characterisation of the bay’s ecosystem. There is no data on the biochemical composition of this species. The purpose of the present investigation is to examine in detail the growth rate, sex distribution, biochemical composition and energy values of the soft-shell clam population in Inner Puck Bay. 2. Material and methods All the samples were taken at Rzucewo every month from May 1994 till April 1995 (except the winter months) (see Figs. 1 and 2, Tab. 1) from an area of 1 m2. Inner Puck Bay Puck Jastarnia Rzucewo 5 Mechelinki Hel 50 Gdynia Sopot 20 GdaÒsk 5 åwibno Fig. 1. The sampling site Individual specimens of M. arenaria were divided into 10 mm length classes; length, height and width were measured with vernier callipers. Seasonal changes in the Mya arenaria (L.) population from Inner Puck Bay 179 25 o 21 20 19 15 13.5 13 11 10 10 8 6 5 5 temperature0 [ C] May June July Aug. Sept. Oct. Nov. Dec. Jan. Feb. Mar. Apr. Fig. 2. Seasonal changes in seawater temperature Table 1. Type of sediment, salinity and depth at the sampling site Sediment type Organic matter Salinity Depth gravel and sand < 1% av. 7.5 PSU ∼ 1 m Wet weight was recorded for the whole organism (both shell and body) and separately for the shell and for the body. The flesh was dried at 60◦C for 48 h to constant dry weight (d.w.). D.w. was determined both in the whole body and in the separate tissues – siphon, muscles, mantle, gills and residue. The biomass of the samples in each month was calculated according to the equation: biomass = mass of sample (g) / area (m2). In our case the area was equal to 1 m2. Microscopic examination of gonadal tissues enabled each individual to be assigned to one of three categories: male, female or immature (Brousseau, 1987). Age classes were determined by counting annuli (Newcombe, 1936). Morphometric relationships were estimated using the following equa- tions: S = aL + b, (1) b W = aL , (2) where S – height or weight, L – length, a, b – parameters of the equation. 180 J. Miąc, M. Groth, M. Wołowicz The parameters a and b were estimated. The relationship between age and shell length was estimated using the von Bertalanffy equation (Brousseau and Baglivo, 1987). The 20–30 mm length group was used in the determination of the biochemical composition of whole organisms in each month. Moreover, in October 1994 and March 1995 the lipid, carbohydrate and glycogen contents were determined in soft tissues, i.e. siphon, muscles, mantle, gills and the residue (containing gonads and hepatopancreas). Protein content was determined according to Lowry’s method (Lowry et al., 1951). Lipids were extracted from the bivalve bodies with 2:1 chloroform-methanol (Blight and Dyer, 1959) and then determined by the Marsh and Weinstein method (1966). The quantities of carbohydrates and glycogen were determined as described by Dubois et al. (1956). The energy values were calculated on the basis of the biochemical composition using standard conversion factors: 39.57 J mg−1 for lipids, 23.65 J mg−1 for proteins and 17.16 J mg−1 for carbohydrates (Brody, 1945). 3. Results and discussion 3.1. An investigation of the population Fig. 3 shows the number of individuals per m2 and the biomass fluctuations in Inner Puck Bay soft-shell clam population in each month. The average density of M. arenaria there was 129 indiv. m−2, which is higher than the results obtained by Munch-Petersen (1973) – av. 96 or 500 500 -2 -2 15% 450 14% 450 400 400 13% 350 350 11% 11% 10% 300 300 10% 250 250 7% 8% 200 200 15% 15% 12% 12% 150 10% 150 9% 9% 10% 9% 100 100 50 50 biomass [g m ] abundance0 [indiv. m ] 0 May June July Aug. Sept. Oct. Nov. Dec. Jan. Feb. Mar. Apr. month abundance biomass Fig. 3. The number of indiv. m−2 and the biomass of M. arenaria samples in each month Seasonal changes in the Mya arenaria (L.) population from Inner Puck Bay 181 Pfitzenmeyer (1972) – av. 100. Further, the biomasses obtained are much higher than those published by Wenne and Wiktor (1982), who found the biomass of M. arenaria in Inner Puck Bay to be 84 g m−2. M. arenaria is a bioecious pelecypod, the sexes of which are distinguish- able only after examination of the gonads. Of 978 individuals examined, 358 were male and 300 female (Tab. 2). It is therefore reasonable to assume equal numbers of the sexes in the population, which is in good agreement with the results obtained by Pfitzenmeyer (1972), Munch-Petersen (1973) and Brousseau (1987). No evidence of hermaphroditism or protandry was forthcoming, which suggests that these are rather rare phenomena in M. arenaria. Coe and Turner (1938) found only 3 hermaphrodites in more than 1000 individuals, Munch-Petersen (1973) found only 1 in 110 individuals examined, and Brousseau (1987) none at all. Table 2. Numbers and percentages of the different sexes of M. arenaria in Inner Puck Bay in each month Month Sex Total male female immature [indiv.] [%] [indiv.] [%] [indiv.] [%] [indiv.] [%] May 41 41 54 54 5 5 100 100 June 51 49 39 37.5 14 13.5 104 100 July 52 45.5 37 32.5 25 22 114 100 September 52 43.5 38 32 29 24.5 119 100 October 33 30 35 31 44 39 112 100 November 44 31.5 22 15.5 75 53 141 100 March 44 26 47 28 78 46 169 100 April 41 34 28 23 51 43 120 100 Total 358 36 300 31 321 33 979 100 Fig. 4 shows the allometric relationship of M. arenaria in the bay. The estimated parameters of the equation linking length and width, length and height, length and total wet weight, length and body wet weight, and length and body dry weight for each month are shown in Tab. 3. It must be pointed out that the result obtained for the Inner Puck Bay population of M. arenaria differs from the data obtained by other investigators (see Tab. 4). The differences between these parameters are attributable to the effects of substrate, temperature, salinity etc. found at all these sites. Analysis of parameter a in the length-weight relationship suggests that M. arenaria spawns during June–July, which is in good agreement with the analysis of biochemical composition. 182 J. Miąc, M. Groth, M. Wołowicz The age-shell length relationship is shown in Fig. 5. Clearly, M. arenaria from Inner Puck Bay grows rapidly during the first years of life, after which the growth rate decreases. This agrees well with the results obtained by others (Munch-Petersen, 1973; Feder and Paul, 1974; Brousseau, 1979; Brousseau and Baglivo, 1987). An inverse relationship exists between growth rate and age, a feature common to a number of bivalves. Growth rates within a species are commonly found to increase with rising temperature. Accordingly, bivalves occurring in lower latitudes attain a smaller max- imum length than those living in higher latitudes.