bioRxiv preprint doi: https://doi.org/10.1101/2021.06.02.446850; this version posted June 5, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Comparative phylogenomic analysis reveals evolutionary genomic 2 changes and novel toxin families in endophytic Liberibacter 3 pathogens 4 5 Yongjun Tan1, Cindy Wang1, Theresa Schneider1, Huan Li1, Robson Francisco de Souza2, 6 Xueming Tang3, Tzung-Fu Hsieh4,5, Xu Wang6,7,8, Xu Li4,5, and Dapeng Zhang1,9,* 7 8 1Department of Biology, College of Arts & Sciences, Saint Louis University, St. Louis, MO 63103 9 2Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 10 São Paulo 05508-900, Brazil 11 3School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China 12 4Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695 13 5Plants for Human Health Institute, North Carolina State University, Kannapolis, NC 28081 14 6Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 15 36849 16 7Alabama Agricultural Experiment Station, Auburn University, Auburn, AL 36849 17 8HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806 18 9Bioinformatics and Computational Biology Program, College of Arts & Sciences, Saint Louis 19 University, St. Louis, MO 63103 20 21 Correspondence and requests for materials should be addressed to D.Z. (email: 22
[email protected]) 23 24 Running title: Comparative Phylogenomics of Liberibacter Pathogens 25 Keywords: Liberibacter pathogens, huanglongbing, zebra chip, toxins, prophages, 26 pathogenesis, evolution, comparative genomics 27 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.06.02.446850; this version posted June 5, 2021.