DOCSLIB.ORG

HHMI Bulletin November 2009 Vol. 22 No. 4

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
HHMI Bulletin November 2009 Vol. 22 No. 4 HHMI BULLETIN N OV. ’09 VOL.22 t NO.04 4000 Jones Bridge Road r Chevy Chase, Maryland 20815-6789 Hu Howard www.hhmi.org HHMI BULLETIN g hes Medical Institute Going Tubular Your spinal cord, the bundle of nerves that carries messages between your brain and the rest of your body, is housed in a hollow tube that started out flat as a pancake. During embryonic development, the flat sheet of cells folds in on itself r and closes up by the fourth week after conception. The same cellular orchestra- tion happens in most vertebrate embryos, including the frog embryo shown here. www.hhmi.or Movement of these cells involves contraction of filaments called actin (green). On the right, they’ve contracted and begun forming a tube, so the green is more concentrated. When genetic control of the rolling process is perturbed, as it has been on the left side of this embryo, actin does not contract and the sheet stays g flat. Failure to form the so-called “neural tube” lies at the root of several human birth defects, including spina bifida. Read about research on neural tube forma- tion and more in “The Most Vulnerable Patients” (page 12). IN THIS ISSUE Kerry Ressler Fights Fear Membrane Awakening Training TAs TO FULL vol. vol. POTENTIAL 22 / no. / no. Chanjae Lee / Wallingford lab Chanjae Lee / Wallingford Researchers aim to protect babies from 04 the complications of prematurity. OBSERVATIONS 24 Like lips around a straw, the membranes around the channels that control water flow into and out of a cell must form a tight seal. Using electron crystallography, researchers produced this image of the water channel called aquaporin-0. The amino acid residues that make up the protein (blue) are arranged in a unique configuration that allows water molecules (red) to pass through. Hydrophobic lipids (yellow) form snug belts around each protein, creating moisture-repellent barriers ©2009 that help channel the water. Nature VISUALIZE THIS You could say membrane channels really caught Rod MacKinnon’s eye. was clear. The conservation of the signature sequence amino acids Trained as a physician, MacKinnon abandoned medicine for a life in in K+ channels throughout the tree of life, from bacteria to higher science after studying, during a medical residency, how potassium eukaryotic cells, implied that nature had settled upon a very special channels enable the heart to contract and relax. A desire to see those solution to achieve rapid, selective K+ conduction across the cell channels—to see how they physically do their job—pushed him into membrane. For me, this realization provided inspiration to want to structural biology, a field of research that proved providential. In 1998, directly visualize a K+ channel and its selectivity filter. I began to he produced the first high-resolution three-dimensional image of a study crystallography, and although I had no idea how I would obtain potassium channel, a feat that garnered MacKinnon the 2003 Nobel funding for this endeavor, I have always believed that if you really Prize in Chemistry. want to do something then you will find a way. Ion selectivity is critical to the survival of a cell. How does nature accomplish high conduction rates and high selectivity at the same From Roderick MacKinnon’s Nobel Prize Lecture. ©The Nobel Foundation time? The answer to this question would require knowing the atomic 2003. Reprinted with permission of the Nobel Foundation. structure formed by the signature sequence amino acids, that much Mike Perry Courtesy of Tamir Gonen and Thomas Walz. Reprinted with permission from Macmillan Publishers Ltd: Gonen and Thomas Walz. Courtesy of Tamir november ’o9 vol. 22 · no. o4 18 12 web only content Visit the online Bulletin to see an audio slideshow of new teaching assistant Joe DePalma as he goes into his first day as a chemistry lab instructor at the University of Delaware and an audio slideshow of Catherine Drennan’s MIT training class for TAs on student diversity. Listen to Charles Sawyers give his view on the value of translational research and watch him move through a typical work day at Memorial Sloan-Kettering Cancer Center. Read about how a model bacterium is showing sci- entists how pathogens go from harmless to deadly: Pascale Cossart is building a library of knowledge on Listeria, and her methods are informing studies of viral and parasitic infections as well. All of this and more at www.hhmi.org/bulletin/nov2009. 30 24 Features Departments COVER STORY PRESIDENT’S LETTER LAB BOOK THE MOST VULNERABLE PATIENTS 03 Season of Change 40 Removing Radiation Roadblock 12 Problems during pregnancy burden 41 Starving for GABA CENTRIFUGE too many newborns with lifelong 42 Blue Baby Blues disabilities. Researchers are 04 From the Outside Looking In finding clues to a safer nine months, 05 Where Past Meets Future ASK A SCIENTIST and a brighter future. 06 Leapin’ Lizards 43 Does the anatomy of freshwater and saltwater fish differ? UPFRONT TAMING FEAR, RISING CALM 08 TOOLBOX 18 A social conscience rooted in the Piecing Together Rotavirus’s Deep South moves Kerry Ressler, Unique Approach 44 Freeze Frame 10 Seeing Spots a psychiatrist and neuroscientist, NOTA BENE to try to ease the consequences of PERSPECTIVES AND OPINIONS 46 News of recent awards and inner city trauma. 34 Terrence Sejnowski other notable achievements 36 MEMBRANE AWAKENING Q&A: What grade in school was OBSERVATIONS 24 your most formative and why? Researchers are taking a more Visualize This holistic look at cell membranes and CHRONICLE the proteins embedded in them— SCIENCE EDUCATION and the surprises keep coming. 38 Rummaging for Science 39 2009 Holiday Lectures on Science: ENHANCING TA PERFORMANCE Exploring Biodiversity 30 Instead of letting teaching assistants sink or swim, risking their failure and the ire of the undergrads, some schools are prescribing a class in how to teach before they get in front of a class. VISIT THE BULLETIN ONLINE FOR ADDITIONAL CONTENT AND ADDED FEATURES: www.hhmi.org/bulletin COVER IMAGE: JASON HOLLEY contributors Change is in the air. For what seems like a while camps. On our website you’ll find two audio now, we’ve been experiencing adjustments in slideshows—one gives you a glimpse into an the way we live, work, and play—some easier MIT training course on diversity, and in the to embrace than others. Here at the Bulletin, other you get to tag along on a University of we’re going through some changes, too. All for Delaware TA’s first day as lab instructor. the better, I think, and I hope you’ll agree. Other stories are moving online as well, With this issue, we’ve gone a little shorter, including one of our usual trio of Upfront arti- by eight pages. We haven’t lost the content cles. With this issue, you can go online to read you are accustomed to seeing, it’s just moved about the recent discoveries of HHMI interna- online—and expanded, in some cases. It made tional research scholar Pascale Cossart on the sense to trim Bulletin costs by slightly reduc- biology of Listeria infection—and watch a short ing the print version, while offering existing video of how the bacteria infect a cell. content and then some on the Web. In coming editions of the Bulletin, we intend By visiting the online version of this issue, for to expand our Web extras, in the form of addi- example, you’ll find a multimedia perspective tional stories and interactive multimedia. We piece by HHMI investigator Charles Sawyers. hope you’ll continue to enjoy our print maga- In this short audio slideshow, you can listen to zine, and visit the online version to learn even Sawyers give his view on the value of transla- more about HHMI’s research and science edu- tional research and watch him move through a cation efforts. Our goal, as always, is to pro- typical work day at Memorial Sloan-Kettering duce engaging content that both informs and Cancer Center. inspires. Drop me a line and let me know how One of our feature articles is shorter, with we’re doing. some added extras on the Web. That feature presents the efforts by some universities to give novice teaching assistants, or TAs, a welcome boost in the form of training courses and boot HHMI TRUSTEES HHMI OFFICERS & SENIOR ADVISORS James A. Baker, III, Esq. Robert Tjian, Ph.D. / President Senior Partner / Baker Botts L.L.P. Craig A. Alexander / V.P. & General Counsel Ambassador Charlene Barshefsky Peter J. Bruns, Ph.D. / V.P. for Grants & Special Programs Senior International Partner Jack E. Dixon, Ph.D. / V.P. & Chief Scientific Officer WilmerHale Joan S. Leonard, Esq. / Senior Counsel to the President Joseph L. Goldstein, M.D. Avice A. Meehan / V.P. for Communications & Public Affairs Regental Professor & Chairman, Department of Molecular Genetics Edward J. Palmerino / V.P. for Finance & Treasurer University of Texas Southwestern Medical Center at Dallas Gerald M. Rubin, Ph.D. / V.P. & Director, Janelia Farm Research Campus Hanna H. Gray, Ph.D., Chairman Landis Zimmerman / V.P. & Chief Investment Officer President Emeritus & Harry Pratt Judson Distinguished Service Professor of History HHMI BULLETIN STAFF The University of Chicago Mary Beth Gardiner / Editor Cori Vanchieri Garnett L. Keith / Story Editor Chairman / SeaBridge Investment Advisors, L.L.C. Jim Keeley / Science Editor Former Vice Chairman & Chief Investment Officer Andrea Widener / Science Education Editor The Prudential Insurance Company of America Patricia Foster / Associate Director of Communications Paul Nurse, F.R.S. for Web & Special Projects President / The Rockefeller University Sarah C.P. Williams / Assistant Editor Alison F. Richard, Ph.D. Maya Pines / Contributing Editor Vice-Chancellor ADDITIONAL CONTRIBUTORS The University of Cambridge Cay Butler, Michelle Cissell, Clayton S.
Load more

Recommended publications
  • Annual Conference Abstracts
    ANNUAL CONFERENCE 14-17 April 2014 Arena and Convention Centre, Liverpool ABSTRACTS SGM ANNUAL CONFERENCE APRIL 2014 ABSTRACTS (LI00Mo1210) – SGM Prize Medal Lecture (LI00Tu1210) – Marjory Stephenson Climate Change, Oceans, and Infectious Disease Prize Lecture Dr. Rita R. Colwell Understanding the basis of antibiotic resistance University of Maryland, College Park, MD, USA as a platform for early drug discovery During the mid-1980s, satellite sensors were developed to monitor Laura JV Piddock land and oceans for purposes of understanding climate, weather, School of Immunity & Infection and Institute of Microbiology and and vegetation distribution and seasonal variations. Subsequently Infection, University of Birmingham, UK inter-relationships of the environment and infectious diseases Antibiotic resistant bacteria are one of the greatest threats to human were investigated, both qualitatively and quantitatively, with health. Resistance can be mediated by numerous mechanisms documentation of the seasonality of diseases, notably malaria including mutations conferring changes to the genes encoding the and cholera by epidemiologists. The new research revealed a very target proteins as well as RND efflux pumps, which confer innate close interaction of the environment and many other infectious multi-drug resistance (MDR) to bacteria. The production of efflux diseases. With satellite sensors, these relationships were pumps can be increased, usually due to mutations in regulatory quantified and comparatively analyzed. More recent studies of genes, and this confers resistance to antibiotics that are often used epidemic diseases have provided models, both retrospective and to treat infections by Gram negative bacteria. RND MDR efflux prospective, for understanding and predicting disease epidemics, systems not only confer antibiotic resistance, but altered expression notably vector borne diseases.
    [Show full text]
  • Female Fellows of the Royal Society
    Female Fellows of the Royal Society Professor Jan Anderson FRS [1996] Professor Ruth Lynden-Bell FRS [2006] Professor Judith Armitage FRS [2013] Dr Mary Lyon FRS [1973] Professor Frances Ashcroft FMedSci FRS [1999] Professor Georgina Mace CBE FRS [2002] Professor Gillian Bates FMedSci FRS [2007] Professor Trudy Mackay FRS [2006] Professor Jean Beggs CBE FRS [1998] Professor Enid MacRobbie FRS [1991] Dame Jocelyn Bell Burnell DBE FRS [2003] Dr Philippa Marrack FMedSci FRS [1997] Dame Valerie Beral DBE FMedSci FRS [2006] Professor Dusa McDuff FRS [1994] Dr Mariann Bienz FMedSci FRS [2003] Professor Angela McLean FRS [2009] Professor Elizabeth Blackburn AC FRS [1992] Professor Anne Mills FMedSci FRS [2013] Professor Andrea Brand FMedSci FRS [2010] Professor Brenda Milner CC FRS [1979] Professor Eleanor Burbidge FRS [1964] Dr Anne O'Garra FMedSci FRS [2008] Professor Eleanor Campbell FRS [2010] Dame Bridget Ogilvie AC DBE FMedSci FRS [2003] Professor Doreen Cantrell FMedSci FRS [2011] Baroness Onora O'Neill * CBE FBA FMedSci FRS [2007] Professor Lorna Casselton CBE FRS [1999] Dame Linda Partridge DBE FMedSci FRS [1996] Professor Deborah Charlesworth FRS [2005] Dr Barbara Pearse FRS [1988] Professor Jennifer Clack FRS [2009] Professor Fiona Powrie FRS [2011] Professor Nicola Clayton FRS [2010] Professor Susan Rees FRS [2002] Professor Suzanne Cory AC FRS [1992] Professor Daniela Rhodes FRS [2007] Dame Kay Davies DBE FMedSci FRS [2003] Professor Elizabeth Robertson FRS [2003] Professor Caroline Dean OBE FRS [2004] Dame Carol Robinson DBE FMedSci
    [Show full text]
  • Medical Advisory Board September 1, 2006–August 31, 2007
    hoWard hughes medical iNstitute 2007 annual report What’s Next h o W ard hughes medical i 4000 oNes Bridge road chevy chase, marylaNd 20815-6789 www.hhmi.org N stitute 2007 a nn ual report What’s Next Letter from the president 2 The primary purpose and objective of the conversation: wiLLiam r. Lummis 6 Howard Hughes Medical Institute shall be the promotion of human knowledge within the CREDITS thiNkiNg field of the basic sciences (principally the field of like medical research and education) and the a scieNtist 8 effective application thereof for the benefit of mankind. Page 1 Page 25 Page 43 Page 50 seeiNg Illustration by Riccardo Vecchio Südhof: Paul Fetters; Fuchs: Janelia Farm lab: © Photography Neurotoxin (Brunger & Chapman): Page 3 Matthew Septimus; SCNT images: by Brad Feinknopf; First level of Rongsheng Jin and Axel Brunger; iN Bruce Weller Blake Porch and Chris Vargas/HHMI lab building: © Photography by Shadlen: Paul Fetters; Mouse Page 6 Page 26 Brad Feinknopf (Tsai): Li-Huei Tsai; Zoghbi: Agapito NeW Illustration by Riccardo Vecchio Arabidopsis: Laboratory of Joanne Page 44 Sanchez/Baylor College 14 Page 8 Chory; Chory: Courtesy of Salk Janelia Farm guest housing: © Jeff Page 51 Ways Illustration by Riccardo Vecchio Institute Goldberg/Esto; Dudman: Matthew Szostak: Mark Wilson; Evans: Fred Page 10 Page 27 Septimus; Lee: Oliver Wien; Greaves/PR Newswire, © HHMI; Mello: Erika Larsen; Hannon: Zack Rosenthal: Paul Fetters; Students: Leonardo: Paul Fetters; Riddiford: Steitz: Harold Shapiro; Lefkowitz: capacity Seckler/AP, © HHMI; Lowe: Zack Paul Fetters; Map: Reprinted by Paul Fetters; Truman: Paul Fetters Stewart Waller/PR Newswire, Seckler/AP, © HHMI permission from Macmillan Page 46 © HHMI for Page 12 Publishers, Ltd.: Nature vol.
    [Show full text]
  • 2018 March Meeting Program Guide
    MARCHMEETING2018 LOS ANGELES MARCH 5-9 PROGRAM GUIDE #apsmarch aps.org/meetingapp aps.org/meetings/march Senior Editor: Arup Chakraborty Robert T. Haslam Professor of Chemical Engineering; Professor of Chemistry, Physics, and Institute for Medical Engineering and Science, MIT Now welcoming submissions in the Physics of Living Systems Submit your best work at elifesci.org/physics-living-systems Image: D. Bonazzi (CC BY 2.0) Led by Senior Editor Arup Chakraborty, this dedicated new section of the open-access journal eLife welcomes studies in which experimental, theoretical, and computational approaches rooted in the physical sciences are developed and/or applied to provide deep insights into the collective properties and function of multicomponent biological systems and processes. eLife publishes groundbreaking research in the life and biomedical sciences. All decisions are made by working scientists. WELCOME t is a pleasure to welcome you to Los Angeles and to the APS March I Meeting 2018. As has become a tradition, the March Meeting is a spectacular gathering of an enthusiastic group of scientists from diverse organizations and backgrounds who have broad interests in physics. This meeting provides us an opportunity to present exciting new work as well as to learn from others, and to meet up with colleagues and make new friends. While you are here, I encourage you to take every opportunity to experience the amazing science that envelops us at the meeting, and to enjoy the many additional professional and social gatherings offered. Additionally, this is a year for Strategic Planning for APS, when the membership will consider the evolving mission of APS and where we want to go as a society.
    [Show full text]
  • Structure & Symmetry
    HAPPY HOLIDAYS ASBMB MEMBERS December 2008 Structure & Symmetry American Society for Biochemistry and Molecular Biology J\\PfliGifk\`ej n`k_*.#'''>=G$kX^^\[FI=Zcfe\j 8 $@C- 9 "@C- : ; >=G$kX^^\[Kil\FI=Zcfe\jXi\kiXej]\Zk\[`ekf?<B)0* Z\ccjXe[k_\kX^^\[gifk\`ejXi\m`jlXc`q\[[li`e^@C$- `e[lZ\[elZc\XikiXejcfZXk`feJK8K*#gXe\c8Xe[9 Xe[`e Ôcfgf[`XXe[jki\jjÔY\i]fidXk`fe8Zk`e#gXe\c:Xe[; % Kil\FI= Fi`>\e\jXclk\j >\efd\n`[\FI=Zcfe\j k_\>=Gg`fe\\ij ]fik_\`iEfY\c ]fikX^^\[gifk\`e\ogi\jj`fe Gi`q\XnXi[ ×:$k\id`eXckX^f]>=G ×J\hl\eZ\m\i`Ô\[Xe[^lXiXek\\[ ×<Xj`cpj_lkkc\[`ekf)'[\jk`eXk`fem\Zkfij ×KiXej]\Zk`fe$i\X[p1('l^gcXjd`[;E8 fi`^\e\%Zfd&fi] ORG-041-GFPTaggedAd_ASBMB_v7.indd 1 10/20/08 12:29:39 PM contents DECEMBER 2008 ON THE COVER: Captivated by the symmetry society news of molecular structure, Sung-Hou Kim has been a 2 From the Editor leader in revealing symmetry 3 President’s Message through his studies in crystallography and 5 Letters to the Editor structural genomics. 30 6 Washington Update 12 Retrospective: Anthony G. San Pietro FASEB releases new Breakthroughs in special interest Bioscience. 6 13 Science’s Role in Foreign Policy 14 ASBMB Round Table: Jim Wells and Mary Woolley 16 Keeping Women in Science 19 Grammar and Writing Tips 2009 meeting 20 The 2009 Fritz Lipmann Lectureship: Douglas C. Rees 21 The 2009 ASBMB Merck Award: John Kuriyan 22 The 2009 FASEB Excellence in Science Award: Susan Lindquist science focus 30 Sung-Hou Kim: Consummate Crystallographer departments 7 News from the Hill 10 Member Spotlight 23 Education and Training A leaky pipeline for women scientists.
    [Show full text]
  • Bonnie Bassler
    LEHMAN AND ALLIS TO RECEIVE 2008 ASBMB AWARDS November 2007 Capecchi Awarded Nobel Prize in Medicine American Society for Biochemistrye mistry and Molecular Biology Scientists helping scientists… It costs no more to choose the very best for your custom peptides and antibodies… Ac-C T P R Q I pS F N F K-OH All peptides are made in our laboratories 1461.640 with the most rigorous QC in the industry – pSFNFK-H3PO4 -98 623.245 We sequence every purified -H PO peptide we manufacture! 3 4 PhD scientists with over 70 years of 623.0 626.5 combined experience in Chemistry, Mass 293.141 72 Cell Biology and Immunology 1218.599 1169.510 1347.658 536.784 740.405 809.426 1317.591 6 246.072 499.227 972.531 400 600 800 1000 1200 1400 Mass Complete antibody protocols and no hidden charges. Phosphospecific antibody experts! Custom peptides up to 100 AAs in length and at purities up to >98%. Peptides for epitope mapping as low as $4/AA. Modifications include phosphorylated amino acids, dye-labeling, cyclic peptides, and peptides with stable isotopes. Experience for yourself why research scientists around the world trust 21st Century Biochemicals for their custom peptides and antibodies! Come speak with our scientists at: Biomedical Research Equipment and Supplies Exhibit at Harvard Medical School Sept. 19 – 20 Society for Neuroscience, San Diego, CA Nov. 3 – 7 American Society for Cell Biology, Washington, DC Dec. 1 – 5 www.21stcenturybio.com 260 Cedar Hill Street, Marlboro, MA 01752 Made in the P: 508.303.8222 Toll-free: 877.217.8238 F: 508.303.8333 E: [email protected] U.S.A.
    [Show full text]
  • The Excludon: a New Concept in Bacterial Antisense RNA-Mediated
    Nature Reviews Microbiology | AOP, published online 24 December 2013; doi:10.1038/nrmicro2934 PROGRESS this paradigm in the context of other, better characterized asRNA-mediated regulatory The excludon: a new concept in mechanisms. The excludon concept describes unusually long asRNAs that inhibit the bacterial antisense RNA-mediated expression of one group of genes while enhancing the expression of a second group gene regulation of genes. Thus, single transcripts have the ability to control divergent operons that often have opposing functions. Nina Sesto, Omri Wurtzel, Cristel Archambaud, Rotem Sorek and Pascale Cossart asRNAs in microbial transcriptomes Abstract | In recent years, non-coding RNAs have emerged as key regulators of gene asRNAs are encoded on one strand of the DNA and overlap a gene that is encoded on expression. Among these RNAs, the antisense RNAs (asRNAs) are particularly the opposite strand. Therefore, these cis- abundant, but in most cases the function and mechanism of action for a particular encoded asRNAs have perfect complementa‑ asRNA remains elusive. Here, we highlight a recently discovered paradigm termed rity to the sense transcript from the opposite the excludon, which defines a genomic locus encoding an unusually long asRNA that DNA strand. The regulatory role of asRNAs spans divergent genes or operons with related or opposing functions. Because these was first reported more than 30 years ago, in the case of plasmid- and transposon-encoded asRNAs can inhibit the expression of one operon while functioning as an mRNA for asRNAs in Escherichia coli, when the asRNAs the adjacent operon, they act as fine-tuning regulatory switches in bacteria.
    [Show full text]
  • Division of Extramural Activities Annual Report 2002
    Division of Extramural Activities Annual Report 2002 National Institutes of Health National Cancer Institute Molecular Diagnosis of Cancer Gene expression profiling using DNA microarrays is a technology that arose as a consequence of the Human Genome Project. With DNA microarrays, it is possible to determine the activity (“expression”) of tens of thousands of genes in parallel on microarray plates. The expression of genes influences the biological behavior of a cell because it dictates which proteins the cell can make and gives cells their unique characteristics. In the DNA microarray images shown at the bottom of the cover illustration, each spot represents a different human gene. Red, yellow, and green spots indicate that a gene is expressed at high, intermediate, and low levels, respectively. The pattern of expression of all of the genes in a cell constitutes its gene expression “profile.” Using DNA microarrays, different types of normal and malignant cells can be distinguished from one another because they have distinct gene expression profiles. Shown at the top of the cover illustration are photomicrographs of lymph node biopsies from two patients with diffuse large B cell lymphoma. The tumor at the right belongs to the germinal center B cell-like subgroup of diffuse large B cell lymphoma. Patients with this lymphoma type have a relatively favorable 5-year survival rate of 59% following multi-agent chemotherapy. The tumor at the left belongs to the activated B cell-like subgroup of diffuse large B cell lymphoma. Patients with this lymphoma type have a less favorable 5-year survival rate of 31%. Although the tumors from these patients were indistinguishable histologically, they had striking differences in their gene expression profiles, as exemplified by the microarray images shown below each lymphoma subgroup.
    [Show full text]
  • NIH Conflict of Interest Regs Revised
    OCTOBEROCTOBER 2005 www.asbmb.org Constituent Society of FASEB AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY NIH Conflict of Interest Regs Revised SEE PAGE 30 FOR NEW CLARA BENSON TRAVEL FELLOWSHIP AWARD Held in conjunction with EB2006 Custom Antibodies Your Way! Choose the protocol that is right for you! QwikScreen ™: 65 day, 2 rabbit protocol - 4 immunizations, 3 bleeds/rabbit (~100ml serum), customer supplied peptide/protein - Options: Peptide synthesis, immunograde Conjugation to carrier u ELISA u u Animal extensionsMS analysis $685 Standard: 80 day, 2 rabbit protocol - 5 immunizations, 5 bleeds/rabbit (~ 200ml ser Options: um), ELISA, customer supplied peptide/pr Peptide synthesis MS Check™ peptide sequence confirmation u HPLC purified peptide Affinity purification otein - Pinnacle: $975 u HPLC and MS analysis u Complete Affinity Purified Protocol- Animal extensions 2 rabbit pr 5 bleeds/rabbitotocol, (~ 200mlepitope serum), design, peptide PhD technical synthesis support, (up to 20mer),5 immunizations, HPLC purified to ~85%, 5+mg peptide to customer, ELISA, evaluation period, affinity purification, and morMS Check™ peptide sequence confirmationNo Hidden Charges! e… - Discounts for Multiple Protocols$1795 , Includes peptide sequencing by CID MS/MS– u Guaranteed Peptide Let our enthusiasm for scienceExpert workTechnical for SupportFidelity! P: 508.303.8222 www.21stcenturybio.com Toll-free: 877.217.8238 F: 508.303.8333 you! E: [email protected] www.asbmb.org AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY OCTOBER
    [Show full text]
  • From Telomeres to Empathy Highlights from the EMBO Meeting 2010 by CRISTINA JIMÉNEZ
    AUTUMN 2010 encounters Newsletter of the European Molecular Biology Organization From telomeres to empathy Highlights from The EMBO Meeting 2010 BY CRISTINA JIMÉNEZ ◗ In the early 1980s, after a meeting at the Gordon Research Conference, Elizabeth Blackburn and Jack Szostak discovered that telo meres include a specifi c DNA sequence. 29 years on, the fortuitous encounter resulted in a Nobel Prize for discovering the structure Elizabeth Frans de Waal Blackburn of molecular caps called telomeres and for working out how they protect chromosomes from degradation. This is only one fi brillation, a condition in Richard example of how necessary meetings can be for the advancement of sci- which there is uncoordinated Losick ence. They provide a perfect setting for junior researchers to approach contraction of the cardiac prospective supervisors – and vice versa. They can lead to new part- muscle of the ventricles in the nerships between research groups working in similar fi elds. And they heart, making them quiver also inspire open discussion and collaboration between institutions. rather than contract properly. The EMBO Meeting, held in September in Barcelona, gathered more Haïssaguerre explained how than 1,300 researchers from a broad scope of disciplines, extending he is currently having great from synthetic, developmental and evolutionary biologists to plant success in curing hundreds of scientists and neuroscientists. “Postdocs and PhD students are the patients every year from this main benefi ciaries of these meetings,” pointed out Luis Serrano, who sort of arrhythmia. Austin co-organized the meeting with Denis Duboule. Smith, the other prize winner, | Barcelona © Christine Panagiotidis The meeting kicked off on Saturday 4 September with Richard Losick gave a lecture on stem cells and the Design principles of pluripotency.
    [Show full text]
  • Smutty Alchemy
    University of Calgary PRISM: University of Calgary's Digital Repository Graduate Studies The Vault: Electronic Theses and Dissertations 2021-01-18 Smutty Alchemy Smith, Mallory E. Land Smith, M. E. L. (2021). Smutty Alchemy (Unpublished doctoral thesis). University of Calgary, Calgary, AB. http://hdl.handle.net/1880/113019 doctoral thesis University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Downloaded from PRISM: https://prism.ucalgary.ca UNIVERSITY OF CALGARY Smutty Alchemy by Mallory E. Land Smith A THESIS SUBMITTED TO THE FACULTY OF GRADUATE STUDIES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY GRADUATE PROGRAM IN ENGLISH CALGARY, ALBERTA JANUARY, 2021 © Mallory E. Land Smith 2021 MELS ii Abstract Sina Queyras, in the essay “Lyric Conceptualism: A Manifesto in Progress,” describes the Lyric Conceptualist as a poet capable of recognizing the effects of disparate movements and employing a variety of lyric, conceptual, and language poetry techniques to continue to innovate in poetry without dismissing the work of other schools of poetic thought. Queyras sees the lyric conceptualist as an artistic curator who collects, modifies, selects, synthesizes, and adapts, to create verse that is both conceptual and accessible, using relevant materials and techniques from the past and present. This dissertation responds to Queyras’s idea with a collection of original poems in the lyric conceptualist mode, supported by a critical exegesis of that work.
    [Show full text]
  • A N N U a L R E P O R T 2 0
    0 1 0 2 Acknowledgements T R HFSPO is grateful for the support of the following organizations: O P Australia E R National Health and Medical Research Council (NHMRC) L Canada A Canadian Institute of Health Research (CIHR) U Natural Sciences and Engineering Research Council (NSERC) N European Union N European Commission - A Directorate General Information Society (DG INFSO) European Commission - Directorate General Research (DG RESEARCH) France Communauté Urbaine de Strasbourg (CUS) Ministère des Affaires Etrangères et Européennes (MAEE) Ministère de l’Enseignement Supérieur et de la Recherche (MESR) Région Alsace Germany Federal Ministry of Education and Research (BMBF) India Department of Biotechnology (DBT), Ministry of Science and Technology Italy Ministry of Education, University and Research (CNR) Japan Ministry for Economy, Trade and Industry (METI) Ministry of Education, Culture, Sports, Science and Technology (MEXT) Republic of Korea Ministry of Education, Science and Technology (MEST) New Zealand Health Research Council (HRC) Norway Research Council of Norway (RCN) Switzerland State Secretariat for Education and Research (SER) United Kingdom The International Human Frontier Science Biotechnology and Biological Sciences Research Program Organization (HFSPO) Council (BBSRC) 12 quai Saint Jean - BP 10034 Medical Research Council (MRC) 67080 Strasbourg CEDEX - France Fax. +33 (0)3 88 32 88 97 United States of America e-mail: [email protected] National Institutes of Health (NIH) Web site: www.hfsp.org National Science Foundation (NSF) Japanese web site: http://jhfsp.jsf.or.jp HUMAN FRONTIER SCIENCE PROGRAM The Human Frontier Science Program is unique, supporting international collaboration to undertake innovative, risky, basic research at the frontiers of the life sciences.
    [Show full text]