bioRxiv preprint doi: https://doi.org/10.1101/2020.03.08.982801; this version posted March 10, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Genetically encoded biosensors for branched-chain amino acid metabolism to monitor 2 mitochondrial and cytosolic production of isobutanol and isopentanol in yeast 3 Yanfei Zhang 1, Sarah K. Hammer 1, Cesar Carrasco-Lopez 1, Sergio A. Garcia Echauri 1, and José 4 L. Avalos * 1, 2, 3 5 6 1 Department of Chemical and Biological Engineering; 2 Andlinger Center for Energy and the 7 Environment; 3 Department of Molecular Biology, Princeton University, Princeton, NJ 8 9 10 11 12 *Corresponding author: José L. Avalos 13 Department of Chemical and Biological Engineering, 14 Princeton University, 15 101 Hoyt Laboratory, William Street, Princeton, NJ 08544, USA 16 Phone office: +1 (609) 258-9881 17 Phone lab: +1 (609) 258-0542 18 Fax: +1 (609) 258-1247 19 Email:
[email protected] 20 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.03.08.982801; this version posted March 10, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 22 Abstract 23 Branched-chain amino acid (BCAA) metabolism can be harnessed to produce many valuable 24 chemicals.