DOCSLIB.ORG

Natriuretic Peptide System Gene Variants Are Associated with Ventricular Dysfunction After Coronary Artery Bypass Grafting Amanda A

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Natriuretic Peptide System Gene Variants Are Associated with Ventricular Dysfunction After Coronary Artery Bypass Grafting Amanda A Ⅵ 2008 BEST ABSTRACTS OF THE MEETING: ANESTHESIOLOGY EDITORS’ PICKS Anesthesiology 2009; 110:738–47 Copyright © 2009, the American Society of Anesthesiologists, Inc. Lippincott Williams & Wilkins, Inc. Natriuretic Peptide System Gene Variants Are Associated with Ventricular Dysfunction after Coronary Artery Bypass Grafting Amanda A. Fox, M.D.,* Charles D. Collard, M.D.,† Stanton K. Shernan, M.D.,‡ Christine E. Seidman, M.D.,§ Jonathan G. Seidman, Ph.D.,͉͉ Kuang-Yu Liu, Ph.D.,* Jochen D. Muehlschlegel, M.D.,* Tjorvi E. Perry, M.D.,* Sary F. Aranki, M.D.,# Christoph Lange, Ph.D.,** Daniel S. Herman,†† Thomas Meitinger, M.D.,‡‡ Peter Lichtner, Ph.D,§§ Simon C. Body, M.B.Ch.B., M.P.H.͉͉͉͉ Downloaded from http://pubs.asahq.org/anesthesiology/article-pdf/110/4/738/368353/0000542-200904000-00013.pdf by guest on 25 September 2021 Background: Ventricular dysfunction (VnD) after primary myocardial failure and ischemia. The authors hypothesized that coronary artery bypass grafting is associated with increased natriuretic peptide system gene variants independently predict hospital stay and mortality. Natriuretic peptides have compen- risk of VnD after primary coronary artery bypass grafting. satory vasodilatory, natriuretic, and paracrine influences on Methods: A total of 1,164 patients undergoing primary coro- nary artery bypass grafting with cardiopulmonary bypass at two institutions were prospectively enrolled. After prospec- This article is featured in “This Month in Anesthesiology.” tively defined exclusions, 697 patients of European descent (76 ᭛ Please see this issue of ANESTHESIOLOGY, page 9A. with VnD) were analyzed. VnD was defined as need for at least 2 new inotropes and/or new mechanical ventricular support This article is accompanied by an Editorial View. Please see: after coronary artery bypass grafting. A total of 139 haplotype- ᭜ Eisenach JH, Schroeder DR: Genomic discoveries in perioper- tagging single nucleotide polymorphisms (SNPs) within 7 genes ative medicine: Educating the audience. ANESTHESIOLOGY 2009; (NPPA, NPPB, NPPC, NPR1, NPR2, NPR3, CORIN) were geno- 110:693–5. typed. SNPs univariately associated with VnD were entered into logistic regression models adjusting for clinical covariates pre- Supplemental digital content is available for this article. Direct dictive of VnD. To control for multiple comparisons, permuta- URL citations appear in the printed text and are available in tion analyses were conducted for all SNP associations. both the HTML and PDF versions of this article. Links to the Results: After adjusting for clinical covariates and multiple digital files are provided in the HTML text of this article on the comparisons within each gene, seven NPPA/NPPB SNPs Journal’s Web site (www.anesthesiology.org). (rs632793, rs6668352, rs549596, rs198388, rs198389, rs6676300, rs1009592) were associated with decreased risk of postopera- –0.010 ؍ tive VnD (additive model; odds ratios 0.44–0.55; P * Instructor, ‡ Associate Professor, ͉͉͉͉ Assistant Professor, Department of 0.036) and four NPR3 SNPs (rs700923, rs16890196, rs765199, Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s rs700926) were associated with increased risk of postoperative .(0.034–0.007 ؍ Hospital, Harvard Medical School, Boston, Massachusetts; † Professor, Baylor VnD (recessive model; odds ratios 3.89–4.28; P College of Medicine Division of Cardiovascular Anesthesia at the Texas Heart Conclusions: Genetic variation within the NPPA/NPPB and Institute, Saint Luke’s Episcopal Hospital, Houston, Texas; § Professor, De- partment of Genetics, Harvard Medical School, Boston, Massachusetts, and NPR3 genes is associated with risk of VnD after primary coro- Division of Cardiology, Brigham and Women’s Hospital, Harvard Medical nary artery bypass grafting. Knowledge of such genotypic pre- School, Boston, Massachusetts; ͉͉ Professor, Department of Genetics, Harvard dictors may result in better understanding of the molecular Medical School, Boston, Massachusetts; # Associate Professor, Division of Cardiac Surgery, Brigham and Women’s Hospital, Harvard Medical School, mechanisms underlying postoperative VnD. Boston, Massachusetts; ** Associate Professor, Harvard School of Public Health, Boston, Massachusetts; †† M.D., Ph.D., degree candidate, Department of Genetics, Harvard Medical School, Boston, Massachusetts; ‡‡ Professor, VENTRICULAR dysfunction (VnD) has been reported Institute of Human Genetics, Helmholtz Zentrum Mu¨nchen German Research 1–3 Center for Environmental Health (GmbH), Neuherberg, Germany; §§ Institute after cardiac surgery in 10–20% of patients and has of Human Genetics, Helmholtz Zentrum Mu¨nchen German Research Center been associated with increased hospital stay and long- for Environmental Health (GmbH), Neuherberg, Germany. term mortality after primary coronary artery bypass graft Received from the Department of Anesthesiology, Perioperative and Pain 2 Medicine, Brigham and Women’s Hospital, Boston, Massachusetts. Submitted (CABG) surgery with cardiopulmonary bypass (CPB). for publication September 12, 2008. Accepted for publication December 30, B-type natriuretic peptide (BNP) is a member of the 2008. Supported by Biosite Incorporated, San Diego, California; a Research Starter Grant from the Society of Cardiovascular Anesthesiologists, Richmond, natriuretic peptide family, which includes three structur- Virginia (to Dr. Fox); a Research Starter Grant from the Foundation for ally homologous peptides: A-type (ANP), BNP, and C- Anesthesia Education and Research, Rochester, Minnesota (to Dr. Fox); 4 Eleanor and Miles Shore Fellowship from Harvard Medical School via the type natriuretic peptides. ANP and BNP are primarily Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and released from atrial and ventricular myocytes in re- Women’s Hospital, Boston, Massachusetts (to Dr. Fox); grant no. K23- HL068774 National Institute of Health, Bethesda, Maryland (to Dr. Body); the sponse to increased cardiac atrial and ventricular wall Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and 4 Women’s Hospital, Boston, Massachusetts; and the Baylor College of Medicine stress. C-type natriuretic peptide is released from vas- 4 Division of Cardiovascular Anesthesia at the Texas Heart Institute, Saint Luke’s cular endothelial cells and other tissues. Natriuretic Episcopal Hospital, Houston, Texas. Presented at the Annual Meeting of the Amer- ican Society of Anesthesiologists, Orlando, Florida, October 20, 2008. peptides bind to two guanylyl cyclase-coupled effector Address correspondence to Dr. Fox: Department of Anesthesiology, Peri- receptors (natriuretic peptide receptors A and B) and a operative and Pain Medicine, Brigham and Women’s Hospital, 75 Francis clearance receptor (natriuretic peptide receptor C). In Street, Boston, Massachusetts 02115. [email protected]. This article may be accessed for personal use at no charge through the Journal Web site, addition to compensatory natriuretic, diuretic, and va- www.anesthesiology.org. sorelaxant properties, natriuretic peptides also have mit- Anesthesiology, V 110, No 4, Apr 2009 738 NATRIURETIC PEPTIDE GENES AND VENTRICULAR DYSFUNCTION 739 igating paracrine influences on myocardial ischemia– genomic control single nucleotide polymorphisms reperfusion injury and cardiovascular remodeling.4–14 (SNPs) indicated non-European ethnicity.25 Circulating plasma BNP levels are used to assess the severity of heart failure and myocardial ischemia and to Data and Blood Collection predict adverse outcomes in patients with acute coronary Data were collected for each enrolled subject during syndromes or undergoing cardiovascular surgery.1,2,15–18 primary hospitalization using a detailed prospectively Natriuretic peptide system gene variants have been as- designed study case report form that includes: (1) pre- sociated with cardiovascular disease states such as hy- operative demographic characteristics, comorbidities, pertension,19–21 stroke,22,23 and myocardial infarction.24 and medications; (2) surgical characteristics; and (3) However, to date, the association between natriuretic postoperative in-hospital events. Data were subject to peptide system gene variants and development of VnD automated range and logic checking as well as additional Downloaded from http://pubs.asahq.org/anesthesiology/article-pdf/110/4/738/368353/0000542-200904000-00013.pdf by guest on 25 September 2021 after cardiac surgery with CPB has not been examined. manual audit of a proportion of records. Knowledge of such genotypic predictors may enhance Plasma samples obtained preoperatively and on post- our understanding of the molecular mechanisms under- operative days 1–5 were stored in vapor-phase liquid lying postoperative VnD. We thus hypothesized that nitrogen until analysis. Plasma BNP and cTnI were mea- variants within the natriuretic peptide precursor protein sured at all time points using immunoassays conducted (NPPA, NPPB, NPPC), receptor (NPR1, NPR2, NPR3), at a single reference laboratory (Biosite, San Diego, CA). and precursor converting enzyme (CORIN) genes are DNA was extracted from white blood cells using stan- independently associated with the risk of postoperative dard procedures. Genotyping was performed using Se- VnD in patients of European descent undergoing pri- quenom MassARRAY® iPLEX (Sequenom, San Diego, mary CABG surgery with CPB. CA) at Helmholtz Zentrum Mu¨nchen (Neuherberg, Ger- many). Raw genotyping signals were analyzed with Spec- troTyper 3.4 software (Sequenom)
Load more

Recommended publications
  • Panoply™ Human NPR3 Over-Expressing Stable Cell Line
    Panoply™ Human NPR3 Over-expressing Stable Cell Line HEK293-NPR3 Lot. No. (See product label) CELL LINE INFORMATION Cat.No. CSC-SC010567 Cell Line Description HEK293-NPR3 cell line is clonally derived from HEK293 cell line, which has been transduced with a lentiviral vector encoding: 1) full length human NPR3 under the CMV promoter, and 2) puromycin resistance gene under the control of PGK promoter. Overexpression of human NPR3 has been quantified by qPCR. Background NPR3 (natriuretic peptide receptor 3) is an atrial natriuretic peptide receptor that in humans is encoded by the NPR3 gene. This protein is one of three natriuretic peptide receptors. It is responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. Host Cell HEK293 Gene Information Gene Name Natriuretic peptide receptor 3 Official Symbol NPR3 Species Homo sapiens (human) GeneID 4883 mRNA Reference NM_000908.3 Protein Reference NP_000899.1 Synonyms NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23 SAFETY AND PACKAGING Mycoplasma Mycoplasma Status: Negative (MycoAlert Kit) Format One frozen vial Storage Liquid nitrogen Safety Considerations The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
    [Show full text]
  • MECHANISMS in ENDOCRINOLOGY: Novel Genetic Causes of Short Stature
    J M Wit and others Genetics of short stature 174:4 R145–R173 Review MECHANISMS IN ENDOCRINOLOGY Novel genetic causes of short stature 1 1 2 2 Jan M Wit , Wilma Oostdijk , Monique Losekoot , Hermine A van Duyvenvoorde , Correspondence Claudia A L Ruivenkamp2 and Sarina G Kant2 should be addressed to J M Wit Departments of 1Paediatrics and 2Clinical Genetics, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, Email The Netherlands [email protected] Abstract The fast technological development, particularly single nucleotide polymorphism array, array-comparative genomic hybridization, and whole exome sequencing, has led to the discovery of many novel genetic causes of growth failure. In this review we discuss a selection of these, according to a diagnostic classification centred on the epiphyseal growth plate. We successively discuss disorders in hormone signalling, paracrine factors, matrix molecules, intracellular pathways, and fundamental cellular processes, followed by chromosomal aberrations including copy number variants (CNVs) and imprinting disorders associated with short stature. Many novel causes of GH deficiency (GHD) as part of combined pituitary hormone deficiency have been uncovered. The most frequent genetic causes of isolated GHD are GH1 and GHRHR defects, but several novel causes have recently been found, such as GHSR, RNPC3, and IFT172 mutations. Besides well-defined causes of GH insensitivity (GHR, STAT5B, IGFALS, IGF1 defects), disorders of NFkB signalling, STAT3 and IGF2 have recently been discovered. Heterozygous IGF1R defects are a relatively frequent cause of prenatal and postnatal growth retardation. TRHA mutations cause a syndromic form of short stature with elevated T3/T4 ratio. Disorders of signalling of various paracrine factors (FGFs, BMPs, WNTs, PTHrP/IHH, and CNP/NPR2) or genetic defects affecting cartilage extracellular matrix usually cause disproportionate short stature.
    [Show full text]
  • Regulation of the Natriuretic Peptide Receptor 2 (Npr2) By
    9768 • The Journal of Neuroscience, November 7, 2018 • 38(45):9768–9780 Development/Plasticity/Repair Regulation of the Natriuretic Peptide Receptor 2 (Npr2) by Phosphorylation of Juxtamembrane Serine and Threonine Residues Is Essential for Bifurcation of Sensory Axons Hannes Schmidt,1,2 Deborah M. Dickey,3 XAlexandre Dumoulin,1 Marie Octave,2 Jerid W. Robinson,3 Ralf Ku¨hn,1,4 Robert Feil,2 Lincoln R. Potter,3 and XFritz G. Rathjen1 1Max Delbru¨ck Center for Molecular Medicine, 13092 Berlin, Germany, 2Interfaculty Institute of Biochemistry, University of Tu¨bingen, 72076 Tu¨bingen, Germany, 3Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, Minnesota 55455, and 4Berlin Institute of Health, 10178 Berlin, Germany cGMP signaling elicited by activation of the transmembrane receptor guanylyl cyclase Npr2 (also known as guanylyl cyclase B) by the ligand CNP controls sensory axon bifurcation of DRG and cranial sensory ganglion (CSG) neurons entering the spinal cord or hindbrain, respectively. Previous studies have shown that Npr2 is phosphorylated on serine and threonine residues in its kinase homology domain (KHD). However, it is unknown whether phosphorylation of Npr2 is essential for axon bifurcation. Here, we generated a knock-in mouse line in which the seven regulatory serine and threonine residues in the KHD of Npr2 were substituted by alanine (Npr2-7A), resulting in a nonphosphorylatable enzyme. Real-time imaging of cGMP in DRG neurons with a genetically encoded fluorescent cGMP sensor or biochemical analysis of guanylyl cyclase activity in brain or lung tissue revealed the absence of CNP-induced cGMP generation in the Npr27A/7A mutant.
    [Show full text]
  • Three New Allelic Mouse Mutations That Cause Skeletal Overgrowth
    Proc. Natl. Acad. Sci. USA Vol. 96, pp. 10278–10283, August 1999 Genetics Three new allelic mouse mutations that cause skeletal overgrowth involve the natriuretic peptide receptor C gene (Npr3) (skeletal development͞endochondral ossification͞intervertebral disc) JEAN JAUBERT*, FRANCIS JAUBERT†,NATALIA MARTIN*, LINDA L. WASHBURN‡,BARBARA K. LEE‡, EVA M. EICHER‡, AND JEAN-LOUIS GUE´NET*§ *Unite´deGe´ne´tique des Mammife`res, Institut Pasteur, 25 Rue du Docteur Roux, 75724 Paris Cedex 15, France; †Service d’Anatomie et de Cytologie Pathologiques, Hoˆpital Necker͞Enfants Malades, 149 Rue de Se`vres, 75743 Paris Cedex 15, France; and ‡The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609-1500 Communicated by Franc¸ois Jacob, Institut Pasteur, Paris, France, June 29, 1999 (received for review April 12, 1999) ABSTRACT In 1979, a BALB͞cJ mouse was identified Natriuretic peptides are implicated in the control of extra- with an exceptionally long body. This phenotype was found to cellular fluid volume, blood pressure, and electrolyte ho- be caused by a recessive mutation, designated longjohn (lgj), meostasis by their natriuretic–diuretic activity. However, ev- that mapped to the proximal region of chromosome 15. idence in vitro has accumulated that indicates that some of Several years later, a mouse with a similarly elongated body these peptides act on the differentiation and proliferation of was identified in an outbred stock after chemical mutagenesis bone cells, i.e., osteoblasts, chondrocytes, and osteoclasts with ethylnitrosourea. This phenotype also was caused by a (14–17). Studies in vivo support the suggestion of a natriuretic- recessive mutation, designated strigosus (stri). The two mu- related bone pathway: mice lacking cGMP-dependent protein tations were found to be allelic.
    [Show full text]
  • A Single-Cell Transcriptome Atlas of the Mouse Glomerulus
    RAPID COMMUNICATION www.jasn.org A Single-Cell Transcriptome Atlas of the Mouse Glomerulus Nikos Karaiskos,1 Mahdieh Rahmatollahi,2 Anastasiya Boltengagen,1 Haiyue Liu,1 Martin Hoehne ,2 Markus Rinschen,2,3 Bernhard Schermer,2,4,5 Thomas Benzing,2,4,5 Nikolaus Rajewsky,1 Christine Kocks ,1 Martin Kann,2 and Roman-Ulrich Müller 2,4,5 Due to the number of contributing authors, the affiliations are listed at the end of this article. ABSTRACT Background Three different cell types constitute the glomerular filter: mesangial depending on cell location relative to the cells, endothelial cells, and podocytes. However, to what extent cellular heteroge- glomerular vascular pole.3 Because BP ad- neity exists within healthy glomerular cell populations remains unknown. aptation and mechanoadaptation of glo- merular cells are key determinants of kidney Methods We used nanodroplet-based highly parallel transcriptional profiling to function and dysregulated in kidney disease, characterize the cellular content of purified wild-type mouse glomeruli. we tested whether glomerular cell type sub- Results Unsupervised clustering of nearly 13,000 single-cell transcriptomes identi- sets can be identified by single-cell RNA fied the three known glomerular cell types. We provide a comprehensive online sequencing in wild-type glomeruli. This atlas of gene expression in glomerular cells that can be queried and visualized using technique allows for high-throughput tran- an interactive and freely available database. Novel marker genes for all glomerular scriptome profiling of individual cells and is cell types were identified and supported by immunohistochemistry images particularly suitable for identifying novel obtained from the Human Protein Atlas.
    [Show full text]
  • New Insights Into Metabolic Homeostasis Keith Tan
    Rockefeller University Digital Commons @ RU Student Theses and Dissertations 2015 Activity Based Profiling: New Insights into Metabolic Homeostasis Keith Tan Follow this and additional works at: http://digitalcommons.rockefeller.edu/ student_theses_and_dissertations Part of the Life Sciences Commons Recommended Citation Tan, Keith, "Activity Based Profiling: New Insights into Metabolic Homeostasis" (2015). Student Theses and Dissertations. Paper 285. This Thesis is brought to you for free and open access by Digital Commons @ RU. It has been accepted for inclusion in Student Theses and Dissertations by an authorized administrator of Digital Commons @ RU. For more information, please contact [email protected]. ACTIVITY BASED PROFILING: NEW INSIGHTS INTO METABOLIC HOMEOSTASIS A Thesis Presented to the Faculty of The Rockefeller University in Partial Fulfillment of the Requirements for the degree of Doctor of Philosophy by Keith Tan June 2015 © Copyright by Keith Tan 2015 ACTIVITY BASED PROFILING: NEW INSIGHTS INTO METABOLIC HOMEOSTASIS Keith Tan, Ph.D. The Rockefeller University 2015 There is mounting evidence that demonstrates that body weight and energy homeostasis is tightly regulated by a physiological system. This system consists of sensing and effector components that primarily reside in the central nervous system and disruption to these components can lead to obesity and metabolic disorders. Although many neural substrates have been identified in the past decades, there is reason to believe that there are numerous unidentified neural populations that play a role in energy balance. Besides regulating caloric consumption and energy expenditure, neural components that control energy homeostasis are also tightly intertwined with circadian rhythmicity but this aspect has received less attention.
    [Show full text]
  • Microrna and Heart Failure
    International Journal of Molecular Sciences Review MicroRNA and Heart Failure Lee Lee Wong 1,†, Juan Wang 1,†, Oi Wah Liew 1, Arthur Mark Richards 1,2,3 and Yei-Tsung Chen 1,* 1 Cardiovascular Research Institute, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, #08-01, MD6 Centre for Translational Medicine, 14 Medical Drive, Singapore 117599, Singapore; [email protected] (L.L.W.); [email protected] (J.W.); [email protected] (O.W.L.); [email protected] (A.M.R.) 2 Cardiac Department, National University Health System, Tower Block Level 9, 1E Kent Ridge Road, Singapore 119228, Singapore 3 Christchurch Heart Institute, Department of Medicine, University of Otago, PO Box 4345, Christchurch 8014, New Zealand * Correspondence: [email protected]; Tel.: +65-9-238-4561 † These authors contributed equally to this work. Academic Editor: William Chi-shing Cho Received: 24 February 2016; Accepted: 23 March 2016; Published: 6 April 2016 Abstract: Heart failure (HF) imposes significant economic and public health burdens upon modern society. It is known that disturbances in neurohormonal status play an important role in the pathogenesis of HF. Therapeutics that antagonize selected neurohormonal pathways, specifically the renin-angiotensin-aldosterone and sympathetic nervous systems, have significantly improved patient outcomes in HF. Nevertheless, mortality remains high with about 50% of HF patients dying within five years of diagnosis thus mandating ongoing efforts to improve HF management. The discovery of short noncoding microRNAs (miRNAs) and our increasing understanding of their functions, has presented potential therapeutic applications in complex diseases, including HF.
    [Show full text]
  • A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain Cell Type Targeting and Functionality
    bioRxiv preprint doi: https://doi.org/10.1101/224881; this version posted November 25, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality Tanya L. Daigle1,4, Linda Madisen1,4, Travis A. Hage1, Matthew T. Valley1, Ulf Knoblich1, Rylan S. Larsen1, Marc M. Takeno1, Lawrence Huang1, Hong Gu1, Rachael Larsen1, Maya Mills1, Alice Bosma-Moody1, La'Akea Siverts1, Miranda Walker1, Lucas T. Graybuck1, Zizhen Yao1, Olivia Fong1, Emma Garren1, Garreck Lenz1, Mariya Chavarha2, Julie Pendergraft1, James Harrington1, Karla E. Hirokawa1, Julie A. Harris1, Medea McGraw1, Douglas R. Ollerenshaw1, Kimberly Smith1, Christopher A. Baker1, Jonathan T. Ting1, Susan M. Sunkin1, Jerome Lecoq1, Michael Z. Lin2, Edward S. Boyden3, Gabe J. Murphy1, Nuno da Costa1, Jack Waters1, Lu Li1, Bosiljka Tasic1, Hongkui Zeng1,* 1Allen Institute for Brain Science, Seattle, WA 98109, USA. 2Departments of Neurobiology and Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA. 3MIT Media Lab and McGovern Institute, Departments of Biological Engineering and Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 4These authors contributed equally *Corresponding author: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/224881; this version posted November 25, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. SUMMARY Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating the study of their function.
    [Show full text]
  • C-Type Natriuretic Peptide Ameliorates Vascular Injury and Improves Neurological Outcomes in Neonatal Hypoxic-Ischemic Brain Injury in Mice
    International Journal of Molecular Sciences Article C-Type Natriuretic Peptide Ameliorates Vascular Injury and Improves Neurological Outcomes in Neonatal Hypoxic-Ischemic Brain Injury in Mice Guofang Shen 1, Shirley Hu 1, Zhen Zhao 2, Lubo Zhang 1 and Qingyi Ma 1,* 1 The Lawrence D. Longo, MD Center for Perinatal Biology, Department of Basic Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA; [email protected] (G.S.); [email protected] (S.H.); [email protected] (L.Z.) 2 Center for Neurodegeneration and Regeneration, Zilkha Neurogenetic Institute and Department of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-909-558-4325; Fax: +1-909-558-4029 Abstract: C-type natriuretic peptide (CNP) is an important vascular regulator that is present in the brain. Our previous study demonstrated the innate neuroprotectant role of CNP in the neonatal brain after hypoxic-ischemic (HI) insults. In this study, we further explored the role of CNP in cere- brovascular pathology using both in vivo and in vitro models. In a neonatal mouse HI brain injury model, we found that intracerebroventricular administration of recombinant CNP dose-dependently reduces brain infarct size. CNP significantly decreases brain edema and immunoglobulin G (IgG) extravasation into the brain tissue, suggesting a vasculoprotective effect of CNP. Moreover, in pri- mary brain microvascular endothelial cells (BMECs), CNP dose-dependently protects BMEC survival Citation: Shen, G.; Hu, S.; Zhao, Z.; Zhang, L.; Ma, Q. C-Type Natriuretic and monolayer integrity against oxygen-glucose deprivation (OGD).
    [Show full text]
  • The Long Non-Coding RNA GHSROS Reprograms Prostate Cancer Cell Lines Toward a More Aggressive Phenotype
    bioRxiv preprint doi: https://doi.org/10.1101/682203; this version posted June 26, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 The long non-coding RNA GHSROS reprograms prostate cancer cell lines 2 toward a more aggressive phenotype 3 4 Patrick B. Thomas1,2,3, Penny L. Jeffery1,2,3, Manuel D. Gahete4,5,6,7,8, Eliza J. Whiteside9,10,†, 5 Carina Walpole1,3, Michelle L. Maugham1,2,3, Lidija Jovanovic3, Jennifer H. Gunter3, 6 Elizabeth D. Williams3, Colleen C. Nelson3, Adrian C. Herington1,3, Raúl M. Luque4,5,6,7,8, 7 Rakesh N. Veedu11, Lisa K. Chopin1,2,3,*, Inge Seim1,2,3,12,* 8 9 1 Ghrelin Research Group, Translational Research Institute- Institute of Health and 10 Biomedical Innovation, School of Biomedical Sciences, Queensland University of 11 Technology (QUT), Woolloongabba, Queensland 4102, Australia. 12 2 Comparative and Endocrine Biology Laboratory, Translational Research Institute-Institute 13 of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland 14 University of Technology, Woolloongabba, Queensland 4102, Australia. 15 3 Australian Prostate Cancer Research Centre–Queensland, Institute of Health and 16 Biomedical Innovation, School of Biomedical Sciences, Queensland University of 17 Technology (QUT), Princess Alexandra Hospital, Translational Research Institute, 18 Woolloongabba, Queensland 4102, Australia. 19 4 Maimonides Institute of Biomedical Research of Cordoba (IMIBIC), Córdoba, 14004, 20 Spain. 21 5 Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, 22 14004, Spain.
    [Show full text]
  • Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands That Promote Axonal Growth
    Research Article: New Research Development Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth Jeremy S. Toma,1 Konstantina Karamboulas,1,ª Matthew J. Carr,1,2,ª Adelaida Kolaj,1,3 Scott A. Yuzwa,1 Neemat Mahmud,1,3 Mekayla A. Storer,1 David R. Kaplan,1,2,4 and Freda D. Miller1,2,3,4 https://doi.org/10.1523/ENEURO.0066-20.2020 1Program in Neurosciences and Mental Health, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada, 2Institute of Medical Sciences University of Toronto, Toronto, Ontario M5G 1A8, Canada, 3Department of Physiology, University of Toronto, Toronto, Ontario M5G 1A8, Canada, and 4Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5G 1A8, Canada Abstract Peripheral nerves provide a supportive growth environment for developing and regenerating axons and are es- sential for maintenance and repair of many non-neural tissues. This capacity has largely been ascribed to paracrine factors secreted by nerve-resident Schwann cells. Here, we used single-cell transcriptional profiling to identify ligands made by different injured rodent nerve cell types and have combined this with cell-surface mass spectrometry to computationally model potential paracrine interactions with peripheral neurons. These analyses show that peripheral nerves make many ligands predicted to act on peripheral and CNS neurons, in- cluding known and previously uncharacterized ligands. While Schwann cells are an important ligand source within injured nerves, more than half of the predicted ligands are made by nerve-resident mesenchymal cells, including the endoneurial cells most closely associated with peripheral axons. At least three of these mesen- chymal ligands, ANGPT1, CCL11, and VEGFC, promote growth when locally applied on sympathetic axons.
    [Show full text]
  • Mediators and Receptors of Chronic Itch in Primates and Humans
    MEDIATORS AND RECEPTORS OF CHRONIC ITCH IN PRIMATES AND HUMANS A Dissertation Submitted to the Temple University Graduate Board In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY by Leigh Ann Nattkemper December 2015 Examining Committee Members: Gil Yosipovitch, MD, Advisory Chair, Department of Dermatology Mary Barbe, PhD, Department of Anatomy and Cell Biology Liselotte Jensen, PhD, Department of Microbiology and Immunology Alan Cowan, PhD, Department of Pharmacology Mark Hoon, PhD, External Member, National Institutes of Health (NIDCR) © Copyright 2015 by Leigh Nattkemper All Rights Reserved ii ABSTRACT Chronic itch has a significant impact on quality of life for millions of patients worldwide, on a level comparable to that of chronic pain. Yet, although there are a host of effective drugs available for pain, there are no therapies that specifically target chronic itch. Current experimental approaches to investigate the pathogenesis of chronic pruritus and to test novel therapeutic agents are largely limited to rodent models. However, rodent models display significant dermatological, neurophysiological, and immunological differences from humans with chronic itch. The disadvantages of the current rodent paradigms call for the design of a valid primate model of chronic itch. For four years, we have monitored scratching behavior in a primate colony (n=35) of Cynomolgus macaques ( Macaca fascicularis ) suffering from idiopathic chronic itch. By comparing molecular and genetic analyses of the primates’ skin to their quantified scratching behavior, we attempted to characterize the underlying mechanisms of chronic itch in this model. Furthermore, the expression of itch-related proteins was examined in both the primate model and in humans with pruritic diseases.
    [Show full text]