Panoply™ Human NPR3 Over-Expressing Stable Cell Line

Panoply™ Human NPR3 Over-expressing Stable Cell Line

HEK293-NPR3 Lot. No. (See product label)

CELL LINE INFORMATION

Cat.No. CSC-SC010567 Cell Line Description HEK293-NPR3 cell line is clonally derived from HEK293 cell line, which has been transduced with a lentiviral vector encoding: 1) full length human NPR3 under the CMV promoter, and 2) puromycin resistance gene under the control of PGK promoter. Overexpression of human NPR3 has been quantified by qPCR. Background NPR3 (natriuretic peptide receptor 3) is an atrial natriuretic peptide receptor that in humans is encoded by the NPR3 gene. This protein is one of three natriuretic peptide receptors. It is responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. Host Cell HEK293

Gene Information

Gene Name Natriuretic peptide receptor 3 Official Symbol NPR3 Species Homo sapiens (human) GeneID 4883 mRNA Reference NM_000908.3 Protein Reference NP_000899.1 Synonyms NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23

SAFETY AND PACKAGING

Mycoplasma Mycoplasma Status: Negative (MycoAlert Kit) Format One frozen vial Storage Liquid nitrogen Safety Considerations The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice

Representative Data

Figure 1. Cell morphology under white light

Human NPR3 mRNA Over-expression Level (2-ddCt) 2500

2000

1500

1000

500 Fold increases in number in increases Fold 0 HEK293-hNPR3-Monoclonal HEK293-Puro Samples

Figure 2. Human NPR3 qPCR data