Product datasheet Anti-Rab9/RAB9A Antibody Catalog Number: A05768

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-Rab9/RAB9A Antibody Name RAB9A Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human Rab9/RAB9A recombinant (Position: K4-D171). Purification Immunogen affinity purified. Observed MW 23KD Dilution Ratios Western blot: 1:500-2000 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What’s more, this gene may involved in the transport of between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-Rab9/RAB9A Antibody Catalog Number: A05768

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2.ICC analysis analysis of anti- RAB9A using anti- RAB9A antibody (A05768).The antibody (A05768). was sample well of each lane detected in immersion was loaded with 50ug of fixed A431 cell line . Cells sample under reducing were stained using the conditions.Lane 1: human Dylight488-conjugated K562 whole cell Anti-rabbit IgG lysates,Lane 2: human Secondary Antibody HepG2 whole cell (green)Catalog # BA1127) lysates,Lane 3: human and counterstained with HEK293 whole cell DAPI (blue). lysates,Lane 4: human HELA whole cell lysates,Lane 5: Rat PC-12 whole cell lysates,Lane 6: Mouse NIH/3T3 whole cell lysates,Use rabbit anti- RAB9A 1:1000, probed with a goat anti- rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RAB9A at approximately 23KD. The expected band size for RAB9A is at 23KD.

Figure 3.Flow cytometry analysis of HELA cell(1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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