bioRxiv preprint doi: https://doi.org/10.1101/631945; this version posted May 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Discovery of interaction-related sRNAs and their targets in the Brachypodium 2 distachyon and Magnaporthe oryzae pathosystem 3 Silvia Zanini1, Ena Šečić1, Tobias Busche2, Jörn Kalinowski2, Karl-Heinz Kogel1* 4 1Institute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, 5 Heinrich-Buff-Ring 26-32, D-35392, Giessen, Germany 6 2Center for Biotechnology, University Bielefeld, Universitätsstraße 27, D-33615 Bielefeld, Germany 7 8 Running title: 9 sRNAs in the Bd-Mo pathosystem 10 11 Email addresses: 12
[email protected] 13
[email protected] 14
[email protected] 15
[email protected] 16
[email protected] 17 18 *Correspondence to 19
[email protected] 20 21 Keywords: 22 Small RNA, cross-kingdom RNAi, bidirectional communication, RNA targets, plant disease, 23 virulence 24 25 Abstract 1 bioRxiv preprint doi: https://doi.org/10.1101/631945; this version posted May 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 26 Microbial pathogens secrete small RNA (sRNA) effectors into plant hosts to aid infection by 27 silencing transcripts of immunity and signaling-related genes through RNA interference (RNAi).