Pharmacognosy Studies of Waltheria Indica L. of the Family Sterculiaceae
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AEGAEUM JOURNAL ISSN NO: 0776-3808 Pharmacognosy Studies of Waltheria indica L. of the Family Sterculiaceae Dr. Ch. Subbalakshmi 1 & * Dr. G. Meerabai2 1Research Scholar, Department of Botany, Sri Krishnadevaraya University, Ananthapur, A.P., India. 2Assistant Professor, Department of Botany, Rayalaseema University, Kurnool, A.P., India. 1Email : sivakesavulu [email protected] 2Email: [email protected] Abstract: All plants produce chemical compounds as part of their normal metabolic activities, and they synthesize a curious variety of phyto chemicals. Some of the bioactive substances that can be derived from plants are flavonoids, alkaloids, carotenoids, tannin, antioxidants and phenolic compounds. They are all having medicinal importance and used as crude drugs in traditional medicine. The knowledge of the action of a drug can be utilized successfully only when the identity, physical nature and chemical constituents of the drug are well known, and pharmacognosy supplies this information. Pharmacognostic studies ensure endorsement of the plants and reproducible quality of herbal products which will shove to wellbeing and effectiveness of natural products. Pharmacognostic parameters include determination of number of stomata (mm-2), stomatal index, total number of epidermal cells (mm-2) and type of trichomes in leaf. Physicochemical studies like ash analysis and moisture contents, fluorescence analysis, extractive values and inorganic mineral analysis verify the identity of plant, the quality and purity of crude drug, Therefore the morphology, macroscopic, microscopic, physical and physiochemical studies of Waltheria indica L. of the family Sterculiaceae was carried out in order to know its Pharmacognostic importance. Key words: Waltheria indica, Pharmacognosy, morphology, physical characters, crude drugs and physico chemical. Volume 8, Issue 7, 2020 http://aegaeum.com/ Page No: 764 AEGAEUM JOURNAL ISSN NO: 0776-3808 1. Introduction: The Indian subcontinent is a vast repository of medicinal plants that are used in traditional medical treatments. Use of plants as a source of medicine has been an ancient practice and is an important component of the health care system in India. Interest in traditional medicines is growing rapidly due to the attention being given to it by the governmental agencies and different NGO comprises of general public and researchers as well as the increased side effects, adverse drug reactions and cost factor of the modern medicines. Pharmacognosy is "the study of the physical, chemical, biochemical, and biological properties of drugs, drug substances, or potential drugs or drug substances of natural origin as well as the search for new drugs from natural sources". The systematic study of natural medicines in terms of purity, potency, consistency and safety has become the major issues in Pharmacognosy. It deals with the collection, identification, preparation, and extraction of a large group of drugs obtained from natural sources, which are used both in orthodox and traditional medicine. Waltheria indica, L. of the family Sterculiaceae is commonly used in traditional medicine against pain, inflammation, diarrhoea, dysentery, conjunctivitis, wounds, abscess, epilepsy, convulsions, anaemia, bladder ailments, astringent, febrifuge, abortifacient and asthma. Therefore the morphology, macroscopic, microscopic, physical and physiochemical studies of W. indica L. was carried out in order to know its Pharmacognostic importance. 2. Materials & Methods: 2.1. Epidermal Studies: The present work deals with the foliar epidermal morphology. Mature fresh leaves or dried leaves from herbarium were used to prepare the epidermal peels. Several techniques were followed for the preparation of epidermal peels and clearing of leaf bits depending upon the texture of leaves. Dried leaves were softened by immersing in water for 24 to 48 hours and they were of same age and size. The leaves were cut into suitable pieces and placed down on a microscopic slide and soaked in a few drops of 5% sodium hypochlorite. One end is held firmly with thumb and the other end scraped gently with a safety razor blade. If necessary extra hypochlorite was added, so that finally a thin clear area was cut off. The piece was placed in a cavity block for a few minutes in sodium hypochlorite and then washed in water filled in a petridish. Then loosely adhering cells were brushed off with the help of pointed hair brush. In case of herbarium material, the leaves were boiled in water for about of 5-1 minutes. The chemical method was followed for the separation of the peels. Diluted nitric acid and chromic acid (5-10%) were used in different proportions. In some cases using three acid treatment (TAT) methods [19] was followed. Epidermal peels were stained in safranin (1%) and mounted in glycerin and made semi-permanent slides by ringing with nail paints. In case of exceptionally hairy leaves, the hairs were removed prior to separation of epidermal peels by covering the leaf surface with stick fast and gently peeling off the gum dried. Epidermal peels which were obtained by these methods were stained with aqueous safranine and mounted on a clear microscopic slide using glycerine and sealed with DPX- mountant. The preparations were examined under microscope and photographs taken by using camera at 40×10x,15×10x,10×10x and 5×10x magnification. The quantitative values of epidermis such as size of epidermal cells ( S.E.S), size of stomata (S.S), epidermal cell frequency/ mm2(E.C.F), stomatal frequency/mm2(S.F), percentage of stomatal types and length and breadth of trichomes on both the surfaces were recorded. For calculating ECF, the epidermal cells as well as stomatal subsidiaries were also counted excluding costal cells. The values are average of derived usually from readings. Stomatal Index is calculated for both the surfaces in case of amphistomatic leaves and abaxial in case of Volume 8, Issue 7, 2020 http://aegaeum.com/ Page No: 765 AEGAEUM JOURNAL ISSN NO: 0776-3808 hypostomatic leaves. Stomatal index was calculated using the formula of Salisbury’s ,1927 [8] method. Where ‘S’ denotes number of stomata per unit area and E number of epidermal cells of the same area. Measurement of the epidermal cell width was taken at the widest point on each cell. Trichome size (length and breadth) is measured in µm. The range of length and mean is recorded. Number of trichomes per square millimetre is calculated on both surfaces. 2.2. Physico-chemical Constants: The procedures recommended in Indian Pharmacopoeia and WHO guidelines were followed to calculate the physico-chemical constants. Physico- chemical parameters such as colour, consistency, pH and percent yield (% w/w). were determined for all plant extracts. 2.2.1. Ash values: Total ash value: The total ash was determined by incinerating 2-3gms. of accurately weighed air dried coarsely powdered drug in a tarred silica crucible which was previously ignited and cooled before weighing, at a temperature not exceeding 4500C. The ignition was repeated and the percentage of ash with reference to air-dried drug was calculated. Water soluble ash: The total ash was boiled for 5min with 25 ml of water. The residue was washed with hot water, ignited for 15min at a temperature not exceeding 4500C, cooled and weighed. This weight was subtracted from the weight of ash, the difference in weight represents the water soluble ash. The percentage of water soluble ash was calculated with reference to air- dried drug. Acid insoluble ash: The ash obtained was boiled with 25 ml of dilute hydrochloric acid for 5min. and filtered through an ash less filter paper. The residue was washed with hot water, ignited, cooled in a desiccator and weighed. The percentage of acid insoluble ash was calculated with reference to air dried drug. Sulphated ash: The sulphated ash was determined by incinerating 1 gm of accurately weighed air dried coarsely powdered drug in a tarred silica crucible which was previously ignited and cooled before weighing at a temperature not exceeding 4500C. The residue was moistened with 1 ml of concentrated sulphuric acid, ignited at 800±250C until all black particles have disappeared. It was then cooled; again sulphuric acid was added and ignited. It was cooled and the percentage of sulphated ash was calculated with reference to air dried drug. The preliminary phytochemical investigations were conducted employing various phytochemical tests and the presence of various phytochemical constituents was detected. 2.2.2. Extractive values: Ethanol soluble extractive: 5gms. of dried coarse powder of plants were macerated with 100ml of 90% ethanol in a closed flask for 24hrs, shaken frequently during 6 hours and allowed to stand for 18hrs. Filtered immediately taking precautions against loss of ethanol. 25ml of the filtrate was evaporated to dryness in a tarred flat bottomed shallow dish. The residue was dried at 1050C and weighed. The percentage of ethanol soluble extractive was calculated with reference to air dried drug. Volume 8, Issue 7, 2020 http://aegaeum.com/ Page No: 766 AEGAEUM JOURNAL ISSN NO: 0776-3808 Water soluble extractive: 5gms of coarse powder was weighed and dissolved in 100ml of water in a stoppered flask, heated at 800C, shaken well and allowed to stand for 10min. It was cooled. 2gms of kieselghur was added and filtered. 5ml of the filtrate was transferred to a tarred evaporating dish and the solvent was evaporated on a water bath. The percentage of water soluble extractive was calculated with reference to air dried drug. 2.2.3. Determination of volatile oil in drug: 50gms of the drug was boiled with water in a Clavenger’s apparatus. The process was continued till no more oil was collected in the graduated tube. The volume of oil was measured and expressed in percentage. 2.2.4. Determination of crude fibre content: About 2gms of the drug was accurately weighed and extracted with ether. Then 200ml of 1.25% sulphuric acid was added and boiled for 30min under reflux. It was filtered and washed with boiling water until free of acid.