Chromosome Numbers of Angiosperms from the Aegean Islands
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PHYTOLOGIA BALCANICA 21 (3): 245 – 293, Sofia, 2015 245 Reliquiae Runemarkianae. Chromosome numbers of angiosperms from the Aegean Islands Arne Strid Bakkevej 6, DK-5853 Ørbæk, Denmark, e-mail: [email protected] Received: October 15, 2015 ▷ Accepted: November 02, 2015 Abstract. Chromosome numbers are listed for 413 species of angiosperms from the Aegean Islands, based on collections and counts by Runemark and co-workers. Name, chromosome number and collection details (incl. geographical coordinates) are listed for each species. In almost all cases vouchers are at LD. Key words: Aegean flora, chromosome number, Greek flora Introduction Hans Runemark (07.01.1927 – 11.12.2014) was an im- lections and field notes by Runemark and co-work- portant explorer of the Aegean flora for several dec- ers amount to ca. 51 000. A large number of plants ades, from 1957 on when he made his first excursions were cultivated in the experimental greenhouses of on Naxos and adjacent islands (Fig. 1). His entire sci- the Lund Botanical Garden, and chromosome num- entific career was spent at the University of Lund, bers were determined on a routine basis. Sweden, which at that time was a good base for botanical re- search with a combi- nation of herbarium collections, cultiva- tion facilities, labora- tory and library. As registered in the Flo- ra Hellenica Database (mostly by Britt Sno- gerup), Greek col- Fig. 1. Hans Runemark (left) in 1969, discussing the fine art of navigation with Dimitris Skopelitis, skipper on the fishing boat Panormitis which was used for several of the Aegean expeditions. 246 Strid, A. • Chromosome numbers of angiosperms from the Aegean Islands Chromosome counts for a total of 277 species the seed collection was given a separate cultivation were reported by Runemark in three articles in the number such as “R-1319”. In some cases, only seeds series “Mediterranean Chromosome Number Re- (or corms/tubers) were collected, and then there ports” (Runemark 1996, 2000, 2006), and perhaps is only a cultivation number. Root tips for chro- an additional 100 were published elsewhere by mosome counts were taken from young cultivated Runemark or co-workers in the context of revising plants; when flowering, the identity of the species particular taxonomic groups, but a large number re- was confirmed by Runemark, or occasionally by mained unpublished. Counts appearing in the three specialists on the particular taxonomic group, and articles in Flora Mediterranea are for taxa belong- a herbarium voucher was usually prepared. Non- ing to families included in vol. 1 and the first half vouchered chromosome counts have been included of vol. 2 of Flora Europaea (Tutin & al. 1964, 1968). in the list only when the identity of the species has It was Runemark’s intention to include all chromo- been established beyond reasonable doubt. some numbers in a major work on the Aegean flo- Slides for chromosome counts were general- ra which unfortunately never materialized. After he ly prepared with the standard method at that time: passed away in December 2014, his large archive of after pre-treatment for 24 hours at 2–4 °C, root chromosome slides and files was handed over to the tips were fixed in the Svalöf modification of the Botanical Museum of the University of Lund. Navashin-Karpechenko fixative, embedded in par- The extensive files with thousands of handwrit- affin, sectioned by microtome and stained over- ten sheets and index cards documenting chromo- night in 1 % crystal violet. In some cases, Feulgen some counts were obtained on loan by the present staining and squash technique was applied. Techni- author in mid-2015. The following list is a critical cal assistance was provided by the staff of the Lund extract from these files, with updated taxonomy Botanical Garden and by lab technicians at the Cy- and nomenclature. The list includes name, soma- to-Taxonomy Lab of the Institute of Systematic Bot- tic chromosome number, collection details and oc- any. Chromosome counts were made by Runemark casionally comments; latitude and longitude values and occasionally by scientific assistants, notably refer to degrees and minutes. No attempt has been Bengt Bentzer, Roland von Bothmer and Lennart made to re-examine the chromosome slides, but Engstrand. After so many years it is not possible to they are available at the Botanical Museum in Lund specify individual contributions. (LD) and can be consulted there; being stained with The list below is arranged alphabetically accord- a very durable method they are likely to remain use- ing to family, genus and species. With few exceptions, ful for several decades. Chromosome counts pub- taxonomy and nomenclature follows Vascular Plants lished in the three articles in Flora Mediterranea of Greece – an Annotated Checklist (Dimopoulos & al. (Runemark 1996, 2000, 2006) are not duplicated in 2013). When more than one sample has been studied the present list. Looking up complete references to for a species, localities are arranged alphabetically other previously published counts would have been according to name of island, and then chronological- very time-consuming and will have to be left to ly according to collection date; in some cases the list readers with an interest in particular genera or spe- is selective when several identical counts were avail- cies. For some of the listed taxa, notably endemics able for the same species in a small area. in the area, there appear to be no previously pub- lished chromosome counts. Results and discussion Material and methods The present article documents chromosome counts for a total of 413 species. About 90 % of the collections In most cases, seeds were taken from herbari- are from the Kiklades, southern Evvia and some of the um specimens collected in the field. Thus, there East Aegean Islands, notably Ikaria. In all, Runemark are collection numbers such as “Runemark 3590” and co-workers have determined chromosome num- or “Runemark & Snogerup 10174”, and a voucher bers for ca. 800 species, or nearly 50 per cent of the flo- specimen is generally preserved at LD. When sown, ra in this area. Phytol. Balcan. 21(3) • Sofia • 2015 247 Acanthaceae 2n = 32 Acanthus spinosus L. Mikonos: St. Anna Bay, siliceous rock, meadow at the 2n = 56 shore, 37°25' N, 25°23' E, Runemark & Nordenstam Naxos: Near the chapel N of Oros Zeus, 600 m, 1960-06-19. Coll. no. 16185 (LD), cult. no. R-3786. 37°02' N, 25°20' E, Runemark & Snoge rup 1958-08- Allium roseum L. 04. Cult. no. R-1488. 2n = 42 Ano Koufonisi: Fields near the principal village, Alliaceae 36°56' N, 25°36' E, Runemark 1957-04-14. Coll. no. Allium guttatum subsp. tenorei (Parl.) Soldano 1042 (LD), cult. no. R-255. 2n = 16 Allium subhirsutum L. Naxos: Kato Potamia, along the small stream, 37°04' N, 2n = 14 25°26' E, Runemark 1957-05-29. Coll. no. 3541 Naxos: E of Myrtia, along a small stream, 37°07' N, (LD), cult. no. R-1174. 25°27' E, Runemark 1957-05-31. Coll. no. 3678 Allium hirtovaginatum Kunth (LD), cult. no. R-211. 2n = 14 Amaryllidaceae Samos: Mt Kerki, the peak and E of the peak, 1000– 1400 m, 37°44' N, 26°37' E, Runemark & Norden- Narcissus tazetta L. stam 1960-08-02. Coll. no. 16921 (LD). 2n = 20 Tria Nisia: the S-island, limestone, garigue, 0–50 m, Naxos: E of Mytria, along a small stream, 37°07' N, 36°17' N, 26°45' E, Runemark & Nordenstam 1960- 25°27' E, Runemark 1957-03-28. Cult. no. R-162. 05-15. Coll. no. 14327 (LD). Naxos: Faneromeni, along a small stream, 50 m, 37°09' N, 25°29' E, Runemark 1957-05-22. Cult. no. Allium luteolum Halácsy R-189. 2n = 16 Keros: The bay N-NE of Andreas, garigue, 36°53' N, Apiaceae 25°38' E, Runemark & Snoge rup 1958-06-09. Coll. Bifora testiculata (L.) Spreng. no. 10814 (LD), cult. no. R-1467. 2n = 22 Naxos: Above Stavros Keramotis up to Fanari, lime- Lipsi: SE of the town, loose schists, partly limestone, stone cliffs, 600–800 m, 37°06' N, 25°31' E, Rune- 37°17' N, 26°46' E, Runemark & Bothmer 1974-05- mark 1957-06-11. Coll. no. 4439 (LD), cult. no. 01. Coll. no. 46593 (LD), cult. no. R-6839. R-5. Naxos: 1–2 km E-NE of Tripodes, 37°04' N, 25°25' E, Allium neapolitanum Cirillo Runemark 1957-04-27. Coll. no. 1752 (LD), cult. 2n = ca. 32 no. R-331. Ikaria: 1-1.5 km W of Petropouli, olive cultivation, Bupleurum aira Snogerup 250 m, 37°36' N, 26°09' E, Runemark & Snoge rup 2n = 14 1958-04-25. Coll. no. 7056 (LD), cult. no. R-2047. Naxos: 2 km W of Psiliammos, 100 m, 37°01' N, Allium pallens L. 25°33' E, Runemark & Snoge rup 1958-06-07. Coll. 2n = 16 no. 10684 (K, LD, UPA, W), cult. no. R-1672. – Melantii: The small island of Voidonisos, litoral schis- Type collection of B. aira. tose rocks, 0–40 m, 37°14' N, 25°56' E, Runemark & Bupleurum gracile d’Urv. Snoge rup 1958-06-14. Coll. no. 11010 (LD), cult. 2n = 14 no. R-2055. Astipalea: The small island of Kounopia, S-part, hard Allium paniculatum L. limestone, garigue, 36°32' N, 26°28' E, Runemark 2n = 16 & Nordenstam 1960-06-01. Coll. no. 15231 (LD, Samos: Mt Kerki, the peak and E of the peak, 1000– UPA). 1400 m, 37°44' N, 26°37' E, Runemark & Norden- Giannisades: The south island, 35°20' N, 26°11' E, stam 1960-08-02. Coll. no. 16950 (LD), cult. no. Runemark & al. 1962-05-19. Coll. no. 18643 (LD), R-3787.