DOCSLIB.ORG

Caractérisation Et Détection Par Des Méthodes Génotypiques Des Agents Bactériens Aéroportés Associés Au Bioterrorisme

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Caractérisation Et Détection Par Des Méthodes Génotypiques Des Agents Bactériens Aéroportés Associés Au Bioterrorisme CARACTÉRISATION ET DÉTECTION PAR DES MÉTHODES GÉNOTYPIQUES DES AGENTS BACTÉRIENS AÉROPORTÉS ASSOCIÉS AU BIOTERRORISME Thèse SANDRA ISABEL Doctorat en microbiologie-immunologie Philosophiae doctor (Ph.D.) Québec, Canada © Sandra Isabel, 2013 ii Résumé ette thèse de doctorat présente quatre études portant sur la détection des C agents bactériens aéroportés associés au bioterrorisme. Tout d‘abord, un rappel historique des guerres biologiques et du bioterrorisme permettra de mieux comprendre cette menace. Pour cibler la problématique actuelle, les agents biologiques, les procédures d‘intervention des organisations de sécurité et de santé publique ainsi que les méthodes de détection seront ensuite introduites. La capacité de détection rapide des agents biologiques est une des lacunes importantes des procédures d‘intervention. La détection et l‘identification de ces agents biologiques nécessitent plusieurs étapes critiques interreliées. Elles consistent en l‘échantillonnage, la préparation des analytes contenues dans des matrices complexes et finalement l‘identification du micro-organisme en décodant des macromolécules signatures, et ce, notamment par des méthodes génotypiques. Certaines particules peuvent potentiellement être employées pour la préparation d‘armes biologiques aéroportées. De plus, des poudres inoffensives (p. ex., farine) peuvent être utilisées pour terroriser certains individus, des organisations ou la population. Ces composés peuvent toutefois interférer avec les méthodes de détection moléculaire. C‘est pourquoi la première étude traite de l‘interférence sur la détection par la PCR de 23 échantillons poudreux ou environnementaux autres. Cet article présente une méthode séparant des spores de Bacillus de matrices poudreuses afin d‘enlever ces particules nuisibles à la détection. La procédure conceptualisée est simple d‘exécution (10 étapes), rapide (≤ 10 minutes), peu coûteuse (~ 10 $) et permet de traiter une plus grande variété d‘échantillons par rapport à l‘art antérieur. En second lieu, une lyse de spores bactériennes basée sur la sonication rapide (45 secondes) a permis d‘extraire iii l‘ADN génomique avec des rendements comparables à une autre méthode de lyse commercialisée performante nécessitant cinq minutes. Le Module fluidique de lyse ultrasonique a été confectionné pour être décontaminé et transféré d‘une zone contaminée à une zone sécurisée, ce qui est un avantage important dans le contexte du bioterrorisme. Le troisième projet concerne le développement d‘un essai de détection basé sur l‘amplification PCR de type large spectre du gène tuf et l‘identification sur biopuce de séquences d‘ADN signatures grâce à une hybridation en microfluidique. Cet essai a permis de détecter rapidement (75 minutes) 12 des 13 agents bactériens aéroportés associés au bioterrorisme listés par les CDC. Finalement, les analyses de séquences du gène cible, tuf, chez le genre Yersinia, ont montré un haut niveau de divergence entre les gènes dupliqués intragénomiques, tufA et tufB (8,3 à 16,2 %). Ce résultat inattendu pourrait montrer les circonstances particulières permettant à des gènes dupliqués d‘acquérir de nouvelles fonctions. De plus, cette étude a permis de faire une analyse phylogénétique de 14 des 17 espèces décrites du genre Yersinia et d‘apporter des informations sur leur classification taxonomique. Prochainement, il serait intéressant de juxtaposer les différentes étapes de détection des agents biologiques présentées ici dans un système intégré d‘analyse totale. Par conséquent, l‘automatisation pourrait faciliter leur utilisation sur le terrain pour détecter et identifier rapidement (~1 h) des agents biologiques associés au bioterrorisme permettant de prendre en charge les victimes plus efficacement et de contribuer à enrayer la propagation. iv Abstract his doctoral thesis presents four studies regarding the detection of airborne T bacterial biothreat agents. First of all, a historical review of biological warfare and bioterrorism will allow for better understanding of this threat. To focus on the current problematic, biological agents, intervention procedures of public security and health organisations, as well as detection methods will then be introduced. The lack of systems for the reliable and rapid detection of biological agents is an important limitation of intervention procedures. Many interrelated steps are required to detect and identify biological agents. They consist of sampling, sample matrix processing, and finally, microbial identification by decoding macromolecule signatures, notably using genotyping methods. Some particles potentially employed to produce airborne biological weapons could interfere with molecular detection methods. Moreover, inoffensive powders (e.g. flour) can be used as hoaxes to terrorise individuals, organisations, or the population. Therefore, the first article studied the interference on PCR detection of 23 powdery or other environmental samples. This study presents a method to separate Bacillus spores from powdery matrices to alleviate the impact of these interfering compounds. The developed procedure is fast (≤10 minutes), inexpensive (~ 10$), allows easy handling (10 steps) and treatment of a wider sample variety compared to prior art. Secondly, a bacterial spore lysis based on rapid sonication (45 seconds) allowed extraction of genomic DNA in yields comparable to a robust commercialised lysis procedure requiring 5 minutes. The Fluidic Ultrasonic Lysis Module can be decontaminated and transferred from a contaminated to a secured area, which is advantageous in the context of bioterrorism. The third project shows the development of a detection assay based on a bacterial broad-spectrum PCR v amplification of the target gene tuf and the identification of signature DNA sequences using a microfluidic microarray system. This assay allowed the rapid (75 minutes) detection of 12 of the 13 airborne bacterial biothreat agents listed by the CDC. Finally, analyses of tuf gene sequences from the Yersinia genus showed a high level of divergence between intra-genomic tufA and tufB sequences (8.3 to 16.2 %). This unexpected result could reveal particular circumstances allowing duplicated genes to acquire new functions. Moreover, this study allowed a phylogenetic analysis of 14 of the 17 described Yersinia species and added information about their taxonomical classification. In the near future, it would be interesting to combine the different biothreat detection steps presented here into a total analysis system. Hence, automation could facilitate its utilisation in the field to detect and identify (~1 h) biothreat agents resulting in more efficient medical management of victims and contribute to stop propagation. vi Avant-propos Il s‘agit d‘une thèse en version électronique. De ce fait, vous pourrez accéder à certaines informations par des hyperliens qui vous redirigeront vers les sites Internet concernés ou bien vers de l‘information contenue ailleurs dans le document. Chaque type de lien a son propre code de couleur. Voici la liste des types de lien que vous rencontrerez dans cette thèse : Références bibliographiques : (Référence 2012). o Ce lien vous redirigera vers la bibliographie à la fin de la thèse. À cet endroit, pour les références électroniques, vous retrouverez un hyperlien vers le site de publication de celles-ci ou du site Internet concerné. Pour les documents non disponibles électroniquement, le numéro de référence ISBN sera accessible. Tableaux et figures inclus dans le document : (Tableau) et (Figure). o Ce lien vous redirigera vers le tableau ou la figure dont il est question dans le texte. Tableaux et figures d‘articles déjà publiés : En ligne : (Tableau) et (Figure). o Certains tableaux ou certaines figures ont déjà été publiés en haute définition. Ceux-ci sont disponibles sur le site Internet de publication. Ce lien vous redirigera à cet endroit. Redirection vers un site Internet : www.redirection.com o Certains liens externes seront inclus dans le texte lorsque pertinents. vii Information sur les publications Les informations sur les publications et la contribution de l‘étudiante à ces publications seront décrites au début de chacun des chapitres correspondants. Chapitre 3 Rapid Filtration Separation-Based Sample Preparation Method for Bacillus Spores in Powdery and Environmental Matrices Chapitre 4 Modular Ultrasonic Lysis System for Rapid Nucleic Acid Extraction and Sample Transfer of Bacillus spores Chapitre 5 Rapid Microarray Microfluidic Screening of Twelve Airborne Bacterial Biothreat Agents Chapitre 6 Divergence among Genes Encoding the Elongation Factor Tu of Yersinia Species Les tableaux et les figures d‘articles déjà publiés ont été renumérotés afin d‘en faciliter le suivi tout au long de cette thèse. Cependant, le numéro original est indiqué en italique et entre crochets. Par exemple, Tableau 6 [Table 1] signifie qu‘il s‘agit du sixième tableau inclus dans la thèse et du premier tableau présenté dans la publication originale. viii Remerciements e voudrais tout d‘abord remercier le Dr Michel G. Bergeron pour l‘opportunité J qu‘il m‘a offerte ainsi que la motivation qui l‘anime et qu‘il a su me transmettre. Je tiens également à remercier mon codirecteur de thèse, le Dr Maurice Boissinot, pour son sens critique ayant grandement amélioré ma formation scientifique. Je voudrais également exprimer ma gratitude aux Drs Éric Leblanc, Régis Peytavi, François Picard, Gale Stewart, Luc Bissonnette, Ann Huletsky et Sachiko Sato pour leur encadrement dans mes projets
Load more

Recommended publications
  • E. Coli (Expec) Among E
    Elucidating the Unknown Ecology of Bacterial Pathogens from Genomic Data Tristan Kishan Seecharran A thesis submitted in partial fulfilment of the requirements of Nottingham Trent University for the degree of Doctor of Philosophy June 2018 Copyright Statement I hereby declare that the work presented in this thesis is the result of original research carried out by the author, unless otherwise stated. No material contained herein has been submitted for any other degree, or at any other institution. This work is an intellectual property of the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the owner(s) of the Intellectual Property Rights. Tristan Kishan Seecharran i Acknowledgements I would like to express my sincere gratitude and thanks to my external advisor Alan McNally and director of studies Ben Dickins for their continued support, guidance and encouragement, and without whom, the completion of this thesis would not have been possible. Many thanks also go to the members of the Pathogen Research Group at Nottingham Trent University. I would like to thank Gina Manning and Jody Winter in particular for their invaluable advice and contributions during lab meetings. I would also like to thank our collaborators, Mikael Skurnik and colleagues from the University of Helsinki and Jukka Corander from the University of Oslo, for their much-appreciated support and assistance in this project and the published work on Yersinia pseudotuberculosis.
    [Show full text]
  • Tese Carlos 2018
    UFRRJ PRÓ-REITORIA DE PESQUISA E PÓS-GRADUAÇÃO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIA, TECNOLOGIA E INOVAÇÃO EM AGROPECUÁRIA TESE Estudo da Microbiota Bacteriana Endofítica de Cana-de-açúcar por Métodos Independentes de Cultivo: Diversidade, Perfil Taxonômico e Predição de Funções de Interesse Biotecnológico Carlos Magno dos Santos 2018 UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO PRÓ-REITORIA DE PESQUISA E PÓS-GRADUAÇÃO PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIA, TECNOLOGIA E INOVAÇÃO EM AGROPECUÁRIA ESTUDO DA MICROBIOTA BACTERIANA ENDOFÍTICA DE CANA- DE-AÇÚCAR POR MÉTODOS INDEPENDENTES DE CULTIVO: DIVERSIDADE, PERFIL TAXONÔMICO E PREDIÇÃO DE FUNÇÕES DE INTERESSE BIOTECNOLÓGICO CARLOS MAGNO DOS SANTOS Sob a Orientação do Pesquisador Stefan Schwab e Coorientação do Pesquisador José Ivo Baldani Tese submetida como requisito parcial para obtenção do grau de Doutor, no Programa de Pós- graduação em Ciência, Tecnologia e Inovação em Agropecuária, Área de Concentração em Agrobiologia Seropédica, RJ Fevereiro, 2018 Santos, Carlos Magno dos, 1990- S237e Estudo da microbiota bacteriana endofítica de cana de-açúcar por métodos independentes de cultivo: diversidade, perfil taxonômico e predição de funções de interesse biotecnológico / Carlos Magno dos Santos Santos. - 2018. 131 f.: il. Orientador: Stefan Schwab. Coorientador: José Ivo Baldani. Tese(Doutorado). -- Universidade Federal Rural do Rio de Janeiro, Ciência, Tecnologia e Inovação em Agropecuária, 2018. 1. Enriquecimento celular. 2. Independente de cultivo. 3. Líquido do apoplasto. 4. Colmo. 5. Sphingomonas. I. Schwab, Stefan, 1975-, orient. II. Baldani, José Ivo, 1953-, coorient. III Universidade Federal Rural do Rio de Janeiro. Ciência, Tecnologia e Inovação em Agropecuária. IV. Título. É permitida a cópia parcial ou total desta Tese, desde que citada a fonte.
    [Show full text]
  • Yersinia Enterocolitica
    TECHNISCHE UNIVERSITÄT MÜNCHEN Lehrstuhl für Mikrobielle Ökologie Regulation und Freisetzung des insektiziden Komplexes in Yersinia enterocolitica Mandy Starke Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.- Prof. Dr. W. Liebl Prüfer der Dissertation: 1. apl. Prof. Dr. T. Fuchs 2. Univ.-Prof. Dr. M. Hofrichter (nur schriftliche Beurteilung) Technische Universität Dresden Univ.-Prof. Dr. S. Scherer (nur mündliche Prüfung) 3. Univ.-Prof. Dr. J. Heesemann (i.R.) Ludwigs-Maximilians-Universität München Die Dissertation wurde am 10.11.2014 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 17.03.2015 angenommen. “Alles Wissen und alle Vermehrung unseres Wissens endet nicht mit einem Schlusspunkt, sondern mit einem Fragezeichen.“ -Hermann Hesse- Inhaltsverzeichnis I Inhaltsverzeichnis ABBILDUNGSVERZEICHNIS ........................................................................................................... V TABELLENVERZEICHNIS .............................................................................................................. VII ABKÜRZUNGSVERZEICHNIS ..................................................................................................... VIII ZUSAMMENFASSUNG ...................................................................................................................
    [Show full text]
  • Priscila Jane Romano De Oliveira Gonçalves Caracterização E
    Priscila Jane Romano de Oliveira Gonçalves Caracterização e avaliação do papel do gene wcbE de Burkholderia seminalis linhagem TC3.4.2R3 na interação microbiana. Tese apresentada ao Programa de Pós- Graduação em Microbiologia do Instituto de Ciências Biomédicas da Universidade de São Paulo, para obtenção do título de Doutora em Microbiologia. Área de concentração: Microbiologia Orientador: Prof. Dr. Welington Luiz de Araújo Versão original. São Paulo 2017 Priscila Jane Romano de Oliveira Gonçalves Characterization and evaluation of the role of wcbE gene from Burkholderia seminalis strain TC3.4.2R3 in microbial interaction. Thesis presented to the Microbiology Department of Instituto de Ciências Biomédicas from Universidade de São Paulo, as requirement for the degree of Doctor of Philosophy in Microbiology. Concentration area: Microbiology Supervisor: Prof. Dr. Welington Luiz de Araújo Original version. São Paulo 2017 RESUMO GONÇALVES, P. J. R. O. Caracterização e avaliação do papel do gene wcbE de Burkholderia seminalis linhagem TC3.4.2R3 na interação microbiana. 2017. 149 f. Tese (Doutorado em Microbiologia) – Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2017. Burkholderia seminalis tem sido encontrada como um micro-organismo não patogênico, promotor de crescimento vegetal, nodulador de raízes, biorremediador, agente de biocontrole e, também, como organismo patogênico em algumas plantas e pacientes com fibrose cística. O gene wcbE codifica uma glicosiltransferase e pertence ao cluster wcb, que está relacionado à síntese de cápsula e interações ambientais. O objetivo deste trabalho foi investigar o papel do gene wcbE e da temperatura nas interações microbianas de B. seminalis TC3.4.2R3. A produção de biofilme, EPS e compostos antifúngicos foi maior a 28 ºC.
    [Show full text]
  • Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
    Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341
    [Show full text]
  • Characterisation of the Type VI Secretion System in Pantoea Ananatis
    Characterisation of the type VI secretion system in Pantoea ananatis BY Shyntum Divine Yufetar Submitted in partial fulfilment of the requirements for the degree Doctor of Philosophy In the Faculty of Natural and Agricultural Sciences, Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, South Africa February 2014 Approved by supervisory committee Teresa A. Coutinho, Ph.D. Lucy N. Moleleki, Ph.D. Stephanus A. Venter, Ph.D. Ian K. Toth, Ph.D. i DECLARATION I declare that the thesis “Characterisation of the type VI secretion system in Pantoea ananatis”, which I hereby submit for the Doctor of Philosophy degree at the University of Pretoria, is my own work and has not previously been submitted by me for a degree at this or any other tertiary institution. SIGNATURE:………………………………………….. DATE:......……….. Shyntum Divine Yufetar i ACKNOWLEDGEMENTS I am deeply grateful to God for having taken me this far in my life. I would want to acknowledge the invaluable supervision of Prof. TA Coutinho, Dr. Lucy Moleleki, Prof. SN Venter and Dr. Ian Toth who guided me professionally. Many thanks also to Prof. Jacques Theron for your invaluable technical assistance, most of all thank you for helping me think outside the box. Many thanks to my siblings and parents for being there for me all these years. Thanks also to Mr John Menjo and his wife Ruth Menjo for providing me an escape from the rigors of academic work during the weekends and holidays. Special thanks to Ms Vimbai Siziba for all her support, you stood by me, listened and supported me through what I consider the hardest part of my PhD studies.
    [Show full text]
  • Antarctic Rahnella Inusitata: a Producer of Cold-Stable Β-Galactosidase Enzymes
    International Journal of Molecular Sciences Article Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes Kattia Núñez-Montero 1,2,†, Rodrigo Salazar 1,3,†, Andrés Santos 1,3,†, Olman Gómez-Espinoza 2,4 , Scandar Farah 1 , Claudia Troncoso 1,3 , Catalina Hoffmann 1, Damaris Melivilu 1, Felipe Scott 5 and Leticia Barrientos Díaz 1,* 1 Laboratory of Molecular Applied Biology, Center of Excellence in Translational Medicine, Universidad de La Frontera, Avenida Alemania 0458, Temuco 4810296, Chile; [email protected] (K.N.-M.); [email protected] (R.S.); [email protected] (A.S.); [email protected] (S.F.); [email protected] (C.T.); [email protected] (C.H.); [email protected] (D.M.) 2 Biotechnology Investigation Center, Department of Biology, Instituto Tecnológico de Costa Rica, Cartago 159-7050, Costa Rica; [email protected] 3 Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile 4 Laboratory of Plant Physiology and Molecular Biology, Institute of Agroindustry, Department of Agronomic Sciences and Natural Resources, Faculty of Agricultural and Forestry Sciences, Universidad de La Frontera, Temuco 4811230, Chile 5 Green Technology Research Group, Facultad de Ingenieria y Ciencias Aplicadas, Universidad de los Andes, Santiago 7620001, Chile; [email protected] * Correspondence: [email protected]; Tel.: +56-45-259-2802 Citation: Núñez-Montero, K.; † These authors contributed equally to this work. Salazar, R.; Santos, A.; Gómez- Espinoza, O.; Farah, S.; Troncoso, C.; Abstract: There has been a recent increase in the exploration of cold-active β-galactosidases, as it Hoffmann, C.; Melivilu, D.; Scott, F.; offers new alternatives for the dairy industry, mainly in response to the current needs of lactose- Barrientos Díaz, L.
    [Show full text]
  • UNIVERSITY of CALIFORNIA Santa Barbara Delivery and Activity Of
    UNIVERSITY OF CALIFORNIA Santa Barbara Delivery and activity of toxic effector domains from contact-dependent growth inhibition systems in Escherichia coli A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Molecular, Cellular, and Developmental Biology by Julia Laura Elizabeth Willett Committee in charge: Professor Christopher S. Hayes, Chair Professor Anthony de Tomaso Professor David Low Professor Denise Montell June 2016 The dissertation of Julia Laura Elizabeth Willett is approved. _____________________________________________ Anthony de Tomaso _____________________________________________ David Low _____________________________________________ Denise Montell _____________________________________________ Christopher S. Hayes, Committee Chair February 2016 Delivery and activity of toxic effector domains from contact-dependent growth inhibition systems in Escherichia coli Copyright © 2016 by Julia Laura Elizabeth Willett iii To Jason iv ACKNOWLEDGEMENTS I would first and foremost like to thank Chris for being a great advisor and for allowing me to investigate a variety of engrossing research questions throughout my graduate work in his lab. I’m appreciative of all the opportunities that I have had to grow as a scientist and writer, present my research at conferences, and interact with incredible seminar speakers and colleagues. His enthusiasm for science and research is contagious. A number of collaborations made this work possible. I’m extremely grateful for our close (both literally and figuratively) collaboration with David Low and his research group, particularly former members Stephanie Aoki, Sanna Koskiniemi, Travis Smith, and Julia Webb. David is a tremendous scientist with whom scientific conversations are always inspiring. I am also grateful for our collaboration with Robert Morse and Celia Goulding at UC Irvine, as their crystallography expertise has provided invaluable insight into the structural world of CDI.
    [Show full text]
  • List of the Pathogens Intended to Be Controlled Under Section 18 B.E
    (Unofficial Translation) NOTIFICATION OF THE MINISTRY OF PUBLIC HEALTH RE: LIST OF THE PATHOGENS INTENDED TO BE CONTROLLED UNDER SECTION 18 B.E. 2561 (2018) By virtue of the provision pursuant to Section 5 paragraph one, Section 6 (1) and Section 18 of Pathogens and Animal Toxins Act, B.E. 2558 (2015), the Minister of Public Health, with the advice of the Pathogens and Animal Toxins Committee, has therefore issued this notification as follows: Clause 1 This notification is called “Notification of the Ministry of Public Health Re: list of the pathogens intended to be controlled under Section 18, B.E. 2561 (2018).” Clause 2 This Notification shall come into force as from the following date of its publication in the Government Gazette. Clause 3 The Notification of Ministry of Public Health Re: list of the pathogens intended to be controlled under Section 18, B.E. 2560 (2017) shall be cancelled. Clause 4 Define the pathogens codes and such codes shall have the following sequences: (1) English alphabets that used for indicating the type of pathogens are as follows: B stands for Bacteria F stands for fungus V stands for Virus P stands for Parasites T stands for Biological substances that are not Prion R stands for Prion (2) Pathogen risk group (3) Number indicating the sequence of each type of pathogens Clause 5 Pathogens intended to be controlled under Section 18, shall proceed as follows: (1) In the case of being the pathogens that are utilized and subjected to other law, such law shall be complied. (2) Apart from (1), the law on pathogens and animal toxin shall be complied.
    [Show full text]
  • Epidemiology and Comparative Analysis of Yersinia in Ireland Author(S) Ringwood, Tamara Publication Date 2013 Original Citation Ringwood, T
    UCC Library and UCC researchers have made this item openly available. Please let us know how this has helped you. Thanks! Title Epidemiology and comparative analysis of Yersinia in Ireland Author(s) Ringwood, Tamara Publication date 2013 Original citation Ringwood, T. 2013. Epidemiology and comparative analysis of Yersinia in Ireland. PhD Thesis, University College Cork. Type of publication Doctoral thesis Rights © 2013, Tamara Ringwood http://creativecommons.org/licenses/by-nc-nd/3.0/ Item downloaded http://hdl.handle.net/10468/1294 from Downloaded on 2021-10-07T12:07:10Z Epidemiology and Comparative Analysis of Yersinia in Ireland by Tamara Ringwood A thesis presented for the Degree of Doctor of Philosophy National University of Ireland, Cork University College Cork Coláiste na hOllscoile Corcaigh Department of Microbiology Head of Department: Prof. Gerald F. Fitzgerald Supervisor: Prof. Michael B. Prentice April 2013 Contents List of Tables .............................................................................................................................................. iv List of figures ............................................................................................................................................. vi Declaration ............................................................................................................................................... viii Acknowledgements ................................................................................................................................
    [Show full text]
  • NZMS Annual Conference 2017
    NZMS Annual Conference 2017 20-23 November, AUT, Auckland PROGRAMME & ABSTRACTS MAJOR SPONSORS: WELCOME Welcome to the 2017 annual meeting of the New Zealand Microbiological Society. This year the theme of the NZ Microbiological Society (NZMS) conference is, “Our well-being and our microbes”. We are highlighting the dependence of our economy on maintaining and developing healthy microbial interactions while minimising diseases of concern for humans, livestock and plants. This conference covers the diverse field of microbiology and its disciplines including agricultural, environmental, food, medical and biotechnology sectors that rely on microbial knowledge, management and products. As our biggest city, Auckland and the surrounding regions has something for everyone, from hot springs to harbours, whale-watching to wine-tasting, mountains to museums and we look forward to hosting your stay here. CONFERENCE COMMITTEE Conference Chairs Gavin Lear (University of Auckland) | [email protected] Brent Seale (Auckland University of Technology) | [email protected] Organising Committee Steve Flint (Massey University) Kim Handley (University of Auckland) Heather Hendrickson (Massey University) Syrie Hermans – Student Representative (University of Auckland) Thomas Proft (University of Auckland) Mike Taylor (University of Auckland) Bevan Weir (Landcare Research) Weiqin Zhuang (University of Auckland) Rosemary Hancock (événements Limited) Conference Manager | [email protected] Lauren Williams (Auckland University of Technology) Event Co-ordinator WHILE AT THE CONFERENCE… o Registration desk contact phone number: Rosemary, ph 021 217 8298 o Please wear your name label to all conference sessions and social functions. These badges identify you as a NZMS conference participant. The venue is a public space and you may be asked to leave the catering areas if we cannot identify you! o Please take note of the location of the exits in all conference rooms and in your accommodation.
    [Show full text]
  • Tierärztliche Hochschule Hannover Untersuchungen Zum Auftreten
    Tierärztliche Hochschule Hannover Untersuchungen zum Auftreten verschiedener bakterieller Zoonoseerreger und zu den Risikofaktoren in norddeutschen Schweinemastbeständen INAUGURAL – DISSERTATION zur Erlangung des Grades einer Doktorin oder eines Doktors der Veterinärmedizin - Doctor medicinae veterinariae - ( Dr. med. vet. ) vorgelegt von Susanne Döhne Marburg/Lahn Hannover 2010 Wissenschaftliche Betreuung: Prof. Dr. L. Kreienbrock, Institut für Biometrie, Epidemiologie und Informationsverarbeitung Prof. Dr. K.-H. Waldmann, Klinik für kleine Klauentiere und forensische Medizin und Ambulatorische Klinik 1. Gutachter: Prof. Dr. L. Kreienbrock, Prof. Dr. K.-H. Waldmann 2. Gutachter: Prof. Dr. T. Blaha Tag der mündlichen Prüfung: 03.11.2010 Diese Dissertation wurde gefördert durch das Bundesministerium für Bildung und Forschung im Rahmen des Projektes FBI-Zoo Veröffentlichungen Erste Ergebnisse dieser Arbeit wurden bereits veröffentlicht: DÖHNE, S., A. VON ALTROCK, R. MERLE, K.-H. WALDMANN u. L. KREIENBROCK (2009): Incidence and antimicrobial susceptibility of Salmonella in fattening pig herds in Northern Germany. In: 14 th ISAH Congress Vechta, 19.-23. Juli 2009, Proceedings Vol.2 DÖHNE, S., A. VON ALTROCK, R. MERLE, K.-H. WALDMANN u. L. KREIENBROCK (2009): Resistenzen von Salmonella spp. in norddeutschen Schweinemastbetrieben. In: DVG-Fachgruppentagung Epidemiologie und Dokumentation: „Krankheitsdynamik in Populationen – Bedeutung von Surveillance und Impfprogrammen“, Gießen, 2.-4. September 2009 Inhaltsverzeichnis 1 Einleitung 13 2 Literaturübersicht
    [Show full text]