Botrytis Cinerea Secondary Inoculum and on the Efficacy of Biological Control Manzoor Ali Abro
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Nitrogen fertilization of the host plant influences susceptibility, production and aggressiveness of Botrytis cinerea secondary inoculum and on the efficacy of biological control Manzoor Ali Abro To cite this version: Manzoor Ali Abro. Nitrogen fertilization of the host plant influences susceptibility, production and aggressiveness of Botrytis cinerea secondary inoculum and on the efficacy of biological control. Agri- cultural sciences. Université d’Avignon, 2013. English. NNT : 2013AVIG0647. tel-02805920v2 HAL Id: tel-02805920 https://tel.archives-ouvertes.fr/tel-02805920v2 Submitted on 29 Apr 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. UUnn té ddee Patho og e Végéta e Unité de Pathologie Végétale Nitrogen fertilization of the host plant influences susceptibility, production and aggressiveness of Botrytis cinerea secondary Nitrogeninoculum andfertilization on the efficacy of the of hostbiological plant control influences. trytis THÈSE Pour l’obtention du Grade de DOCTEUR DE L’UNIVERSITÉ D’AVIGNON ET DES PAYS DE VAUCLUSE Faculté des Sciences c Présentée et soutenue publiquement par Manzoor Ali ABRO Le 7 Mars 2013 devant le Jury Christiane Raynal, Ingènieur Agronome, Ctifl Lanxade Examnetuer Christophe Robin, Director de recherche INRA Nancy Rapporteur Essaid A. Barka, Professeur Université de Reims Rapporteur Mohamed El Maataoui, Professeur Université d’Avignon Directeur de thèse Philippe Nicot, Chargé de recherche, INRA-Avignon Co-directeur de thèse ACKNOWLEDGMENTS I wish to express profound and unprecedented gratitude to my worthy teacher and supervisor Dr Philippe NICOT research scientist Phytopathology Unit INRA Avignon, France for his invaluable suggestions guidance and constant encouragement towards me during studies and provided a continuous potential of investigations. I express my heart felt gratitude for his generous consideration and indomitable patient shown to me. I feel highly obliged him for entire assistance during this study. I am highly thankful to my director of thesis Professor Mohamed El Maataoui for his help during entire period of my studies especially histological techniques and microscopy at the University of Avignon. I am grateful to Francois LECOMPTE, Marc BARDIN for his constant assistance and valuable suggestions throughout the studies for systemic presentation of the work. I shall be failing in my duty if I do not record my indebtness to Mr Sakhr AJOUZ at Phytopathology unit INRA Avignon for his co-operation and time to time suggestions regarding this study. Thanks are also extended to, Joel BERAUD, Francois Debruni, Clair FROULET, Michel RIQUEAU and, Patricio CASTRO, Arshad Ali, Jonathon GAUDIN and other persons of the unit for their support during this study. I wish also thankful and deeply indebted to my sincere parents my wife and children and loving family members for their blessing and moral support which enabled me to achieve the highly ideals of my academic life. Manzoor Ali Abro 2 Table of contents GENERAL INTRODUCTION ............................................................................................................ 5 Objectives of the thesis work: ............................................................................................................. 7 Chapter I: Review of literature ................................................................. Erreur ! Signet non défini. 1. Botrytis cinerea: causal agent of grey mould: ................................................................................ 10 1.1 Taxonomic position: .................................................................................................................... 11 1.2. Life cycle of the pathogen: ......................................................................................................... 12 1.3. Pathogenicity and host range: ..................................................................................................... 13 1.4. Symptoms: .................................................................................................................................. 14 1.5. Factors affect the development of the pathogen: ........................................................................ 15 1.5.1. Nutrient requirements: ......................................................................................................... 15 1.5.2. Effect of light: ..................................................................................................................... 16 1.5.3 Climatic factors: ................................................................................................................... 17 2. Strategies for protecting plants against B. cinerea ....................................................................... 18 2.1. Chemical control ........................................................................................................................ 18 2.2. Curtural control .......................................................................................................................... 19 2.3. Biological control ....................................................................................................................... 19 3. Effect of nutrition on the susceptibility of plants to Pathogens ................................................... 22 3.1 Effect of nitrogen nutrition on the host pathogen relationship .............................................. 23 3.2 Relationship between the form of nitrogen and host susceptibility ....................................... 23 3.3 Biological interactions between nutrition and soil microorganisms. ..................................... 25 3.4 Trophic host-pathogen interactions ....................................................................................... 26 3.5 Effect of nitrogen nutrition on the capacity of host defence ................................................. 28 3.6 Effect of nitrogen on the development of plant disease ........................................................ 30 4. Relationship between nutritional status of the host plant and Botrytis cinerea ......................... 31 4.1 Nitrogen nutrition of the host and Botrytis ............................................................................ 31 4.2 Effect of form of nitrogen on severity of B. cinerea ............................................................. 33 4.3 Nitrogen increases the susceptibility of crops to B. cinerea .................................................. 33 4.4 Reduction of plant susceptibility to Botrytis cinerea with nitrogen fertilization .................. 35 3 4.5 High N nutrition increase the susceptibility of crops during storage .................................... 36 5. Effect of nitrogen nutrition on fungal sporulation ............................................................... 37 5.1 Effect of type of substrates on the sporulation of different fungi .......................................... 39 5.2 Effect of nutrient substrate on aggressiveness of fungi ......................................................... 40 5.3 Effect of nitrogen supply on sugars, acids carotinoids and phenolic compounds. ................ 40 5.4 Effect of nitrogen nutrition on growth, transpiration and nutrient uptake of the................... 42 6 Effect of nutrition on the enhancement of biological control .................................................. 44 7 Possible mechanisms involved in disease suppression by nitrogen ......................................... 47 7.1 Physiology of the plant .......................................................................................................... 47 7.2 Pathogen growth and virulence ............................................................................................. 49 7.3 Modification of the biotic or a biotic environment ................................................................ 50 7.4 Effect of nitrogen on gene expression of fungal pathogens .................................................. 50 Chapter II: Contrasted response of Botrytis cinerea isolates developing on tomato plants grown under different nitrogen nutrition regimes.............................................................. Erreur ! Signet non défini. Chapeter III: Can plant sugars mediate the effect of nitrogen fertilization on lettuce susceptibility to two necrotrophic pathogens : Botrytis cinerea and Sclerotinia sclerotiorum ?………………………….. ...................................................................................................................... Erreur ! Signet non défini. Chapter 1V: Nitrogen fertilization of the host plant influences production and pathogenicity of Botrytis cinerea secondary inoculum ………………………………;………….Erreur ! Signet non défini. Chapter V: Enhancement of biocontrol efficacy against Botrytis cinerea through the manipulation of nitrogen fertilization of tomato plants……………………………………………;…………. ... Erreur ! Signet non défini. Chapter VI: Cyto-histological evidences for the influence of nitrogen fertilization on