High-Throughput Film-Densitometry
HIGH-THROUHGPUT FILM-DENSITOMETRY: AN EFFICIENT APPROACH TO GENERATE LARGE DATA SETS Dieter Typke1, Robert A. Nordmeyer2, Arthur Jones2, Juyoung Lee1, Agustin Avila-Sakar3, Kenneth H. Downing1, and Robert M. Glaeser1,3 1Life Sciences Division, Donner Laboratory, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720 2Engineering Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720 3Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 Corresponding Author: Robert M. Glaeser Lawrence Berkeley National Laboratory University of California, Berkeley Berkeley, CA 94720 Phone: 510-642-2905 Fax: 510-486-6488 1 ABSTRACT A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to “near-to-perfect” performance. Due to the large area of the standard electron microscope film that can be digitized by the commercial densitometer (up to 10,000x13,680 pixels with an appropriately coded holder),
[Show full text]