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Ectomycorrhizal Fungal Community Structure in a Young Orchard of Grafted and Ungrafted Hybrid Chestnut Saplings
Mycorrhiza (2021) 31:189–201 https://doi.org/10.1007/s00572-020-01015-0 ORIGINAL ARTICLE Ectomycorrhizal fungal community structure in a young orchard of grafted and ungrafted hybrid chestnut saplings Serena Santolamazza‑Carbone1,2 · Laura Iglesias‑Bernabé1 · Esteban Sinde‑Stompel3 · Pedro Pablo Gallego1,2 Received: 29 August 2020 / Accepted: 17 December 2020 / Published online: 27 January 2021 © The Author(s) 2021 Abstract Ectomycorrhizal (ECM) fungal community of the European chestnut has been poorly investigated, and mostly by sporocarp sampling. We proposed the study of the ECM fungal community of 2-year-old chestnut hybrids Castanea × coudercii (Castanea sativa × Castanea crenata) using molecular approaches. By using the chestnut hybrid clones 111 and 125, we assessed the impact of grafting on ECM colonization rate, species diversity, and fungal community composition. The clone type did not have an impact on the studied variables; however, grafting signifcantly infuenced ECM colonization rate in clone 111. Species diversity and richness did not vary between the experimental groups. Grafted and ungrafted plants of clone 111 had a diferent ECM fungal species composition. Sequence data from ITS regions of rDNA revealed the presence of 9 orders, 15 families, 19 genera, and 27 species of ECM fungi, most of them generalist, early-stage species. Thirteen new taxa were described in association with chestnuts. The basidiomycetes Agaricales (13 taxa) and Boletales (11 taxa) represented 36% and 31%, of the total sampled ECM fungal taxa, respectively. Scleroderma citrinum, S. areolatum, and S. polyrhizum (Boletales) were found in 86% of the trees and represented 39% of total ECM root tips. The ascomycete Cenococcum geophilum (Mytilinidiales) was found in 80% of the trees but accounted only for 6% of the colonized root tips. -
Phd. Thesis Sana Jabeen.Pdf
ECTOMYCORRHIZAL FUNGAL COMMUNITIES ASSOCIATED WITH HIMALAYAN CEDAR FROM PAKISTAN A dissertation submitted to the University of the Punjab in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in BOTANY by SANA JABEEN DEPARTMENT OF BOTANY UNIVERSITY OF THE PUNJAB LAHORE, PAKISTAN JUNE 2016 TABLE OF CONTENTS CONTENTS PAGE NO. Summary i Dedication iii Acknowledgements iv CHAPTER 1 Introduction 1 CHAPTER 2 Literature review 5 Aims and objectives 11 CHAPTER 3 Materials and methods 12 3.1. Sampling site description 12 3.2. Sampling strategy 14 3.3. Sampling of sporocarps 14 3.4. Sampling and preservation of fruit bodies 14 3.5. Morphological studies of fruit bodies 14 3.6. Sampling of morphotypes 15 3.7. Soil sampling and analysis 15 3.8. Cleaning, morphotyping and storage of ectomycorrhizae 15 3.9. Morphological studies of ectomycorrhizae 16 3.10. Molecular studies 16 3.10.1. DNA extraction 16 3.10.2. Polymerase chain reaction (PCR) 17 3.10.3. Sequence assembly and data mining 18 3.10.4. Multiple alignments and phylogenetic analysis 18 3.11. Climatic data collection 19 3.12. Statistical analysis 19 CHAPTER 4 Results 22 4.1. Characterization of above ground ectomycorrhizal fungi 22 4.2. Identification of ectomycorrhizal host 184 4.3. Characterization of non ectomycorrhizal fruit bodies 186 4.4. Characterization of saprobic fungi found from fruit bodies 188 4.5. Characterization of below ground ectomycorrhizal fungi 189 4.6. Characterization of below ground non ectomycorrhizal fungi 193 4.7. Identification of host taxa from ectomycorrhizal morphotypes 195 4.8. -
AR TICLE New Sequestrate Fungi from Guyana: Jimtrappea Guyanensis
IMA FUNGUS · 6(2): 297–317 (2015) doi:10.5598/imafungus.2015.06.02.03 New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., ARTICLE Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales) Matthew E. Smith1, Kevin R. Amses2, Todd F. Elliott3, Keisuke Obase1, M. Catherine Aime4, and Terry W. Henkel2 1Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA 2Department of Biological Sciences, Humboldt State University, Arcata, CA 95521, USA; corresponding author email: Terry.Henkel@humboldt. edu 3Department of Integrative Studies, Warren Wilson College, Asheville, NC 28815, USA 4Department of Botany & Plant Pathology, Purdue University, West Lafayette, IN 47907, USA Abstract: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus Key words: cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected Boletineae in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Caesalpinioideae Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea Dipterocarpaceae (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) ectomycorrhizal fungi and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and gasteroid fungi micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Guiana Shield Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera. Article info: Submitted: 31 May 2015; Accepted: 19 September 2015; Published: 2 October 2015. INTRODUCTION 2010, Gube & Dorfelt 2012, Lebel & Syme 2012, Ge & Smith 2013). -
Catálogo De Los Hongos Del Volcán De Tequila, Municipio De Tequila, Jalisco, México
Núm. 45: 15-33 Enero 2018 ISSN electrónico: 2395-9525 Polibotánica ISSN electrónico: 2395-9525 [email protected] Instituto Politécnico Nacional México http:www.polibotanica.mx CATÁLOGO DE LOS HONGOS DEL VOLCÁN DE TEQUILA, MUNICIPIO DE TEQUILA, JALISCO, MÉXICO FUNGI CATALOGUE OF THE TEQUILA VOLCANO, MUNICIPALITY OF TEQUILA, JALISCO, MEXICO Rodríguez-Alcántar, O.; D. Figueroa-García, y M.J. Herrera-Fonseca CATÁLOGO DE LOS HONGOS DEL VOLCÁN DE TEQUILA, MUNICIPIO DE TEQUILA, JALISCO, MÉXICO FUNGI CATALOGUE OF THE TEQUILA VOLCANO, MUNICIPALITY OF TEQUILA, JALISCO, MEXICO Núm. 45: 15-33, México. Enero 2018 Instituto Politécnico Nacional DOI: 10.18387/polibotanica.45.3 15 Núm. 45: 15-33 Enero 2018 ISSN electrónico: 2395-9525 CATÁLOGO DE LOS HONGOS DEL VOLCÁN DE TEQUILA, MUNICIPIO DE TEQUILA, JALISCO, MÉXICO FUNGI CATALOGUE OF THE TEQUILA VOLCANO, MUNICIPALITY OF TEQUILA, JALISCO, MEXICO O. Rodríguez-Alcántar/[email protected] D. Figueroa-García M.J. Herrera-Fonseca Rodríguez-Alcántar, O.; D. Figueroa-García, Departamento de Botánica y Zoología, y M.J. Herrera-Fonseca Universidad de Guadalajara Apartado postal 1-139, 45101 Zapopan, Jalisco, México CATÁLOGO DE LOS HONGOS DEL VOLCÁN DE TEQUILA, MUNICIPIO DE RESUMEN: Se presenta una lista taxonómica de 305 taxa de hongos del Volcán TEQUILA, JALISCO, MÉXICO de Tequila, área localizada en la porción centro occidente del estado de Jalisco. El material listado, se recolectó principalmente en bosque de encino y bosque mixto de pino-encino. Del total de especies, 274 son Basidiomycota y 31 Ascomycota del FUNGI CATALOGUE OF reino Fungi. Los órdenes mejor representados fueron los Agaricales (106), THE TEQUILA VOLCANO, MUNICIPALITY OF Polyporales (46) y Boletales (38) dentro de los Basidiomycota; y Pezizales (21) e TEQUILA, JALISCO, Hypocreales (5) para Ascomycota. -
A New Genus, Rubroboletus, to Accommodate Boletus Sinicus and Its Allies
Phytotaxa 188 (2): 061–077 ISSN 1179-3155 (print edition) www.mapress.com/phytotaxa/ PHYTOTAXA Copyright © 2014 Magnolia Press Article ISSN 1179-3163 (online edition) http://dx.doi.org/10.11646/phytotaxa.188.2.1 A new genus, Rubroboletus, to accommodate Boletus sinicus and its allies KUAN ZHAO1,2, GANG WU1 & ZHU L. YANG1,* 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kun- ming 650201, China 2 University of Chinese Academy of Sciences, Beijing 100049, China *e-mail: [email protected] Abstract Rubroboletus is erected as a new genus to accommodate Boletus sinicus and its allies based on morphological and molecular evidence. Morphologically, Rubroboletus differs from the remaining genera in Boletaceae by the combination of a reddish pileal surface, an orange-red to blood red surface of the hymenophore, yellow tubes, pink to red reticula or spots on the yellow background of the stipe, a bluish color-change when injured, a non-amyloid context, smooth spores which are olive- brown in deposit, and an interwoven trichodermal pileipellis. Our phylogenetic analyses based on five gene markers (ITS, nrLSU, tef1-α, rpb1 and rpb2) recognized eight species in the genus, including one new species and seven new combina- tions. A key to the eight species is provided. Keywords: Boletes, New taxa, Rubroboletus, Phylogeny, Taxonomy Introduction The genus Boletus L. (1753: 1176) has been widely studied by mycologists from all over the world (Fries 1838; Murrill 1909; Singer 1947, 1986; Dick 1960; Hongo 1960; Smith & Thiers 1971; Corner 1972; Nilson & Persson 1977; Pegler & Young 1981; Zang 1983, 2006; Høiland 1987; Both 1993, 1998; Watling & Li 1999; Li & Song 2000; Binder & Bresinsky 2002; Horak 2005, 2011; Binder & Hibbett 2006; Ortiz-Santana et al. -
Bryn Tjader Mason Dentinger
BRYN T. M. DENTINGER [email protected] [email protected] dentingerlab.org dikaryon.wordpress.com @NHMUMycology Curator of Mycology Associate Professor Natural History Museum of Utah School of Biological Sciences 301 Wakara Way University of Utah Salt Lake City, UT 84108 257 South 1400 East, Rm. 201 Office: 801-585-1506 Salt Lake City, UT 84112 Lab: 801-587-5729 Office: LS207 Tel: 801-213-3695 Languages: English (native, preferred), Spanish (competent) ACADEMIC APPOINTMENTS 2016-current Associate Professor & Curator of Mycology, School of Biological Sciences & Natural History Museum of Utah, University of Utah (Salt Lake City, UT) 2014-2016 Senior Research Leader, Comparative Fungal Biology, Royal Botanic Gardens, Kew (London, UK) (managed three full-time junior and senior research staff) 2012-2014 Head of Mycology, Royal Botanic Gardens, Kew (London, UK) (managed eight full-time junior and senior research and curatorial staff) 2010-2012 Senior Researcher in Mycology, Royal Botanic Gardens, Kew (London, UK) 2011-2016 Honorary Lecturer, Institute of Biology, Environment and Rural Sciences, Aberystwyth University (Aberystwyth, Wales, UK) ACADEMIC TRAINING 2009-2010 Postdoctoral Research Associate, Center for Ecology and Evolutionary Biology, University of Oregon (Eugene, OR) 2007-2009 Postdoctoral Research Associate, Department of Natural History & Department of Ecology and Evolutionary Biology, Royal Ontario Museum, University of Toronto (Toronto, ON) 2007 Research Assistant, Minnesota County Biological Survey EDUCATION 2007 -
Secondo Semestre 2017 Stampa: Giugno 2017
N “Naturalmente – notiziario di Nuova Micologia” Numero 14: secondo semestre 2017 Stampa: giugno 2017 INDICE INDICE pag. Editoriale................................................................................................. 3 pag. Naturalmente Editoriale................................................................................................. ... FUNGHI .................................................................. 4 3 Giro Naturalmente d’orizzonte ...................................................................................... .................................................................. 6 4 I funghi Giro d’orizzonte di Villa Ada ...................................................................................... ................................................................................. 8 6 Monitoraggio I funghi di Villa della Ada flora ................................................................................. fungina a La Chanéaz (CH) ............................. 11 8 Il CoprinusMonitoraggio comatus, della unflora potenziale fungina a amico La Chanéaz per la nostra (CH) .............................salute .............. 16 11 Concorso Il Coprinus fotografico comatus, 2016 un potenziale..................................................................... amico per la nostra salute .............. 18 16 Schede: Concorso Le erbe fotografico dei nostri 2016 campi, ..................................................................... Foeniculum vulgare ......................... 19 18 L’angolo Schede: -
Boletales – Boletaceae S.L. (26 October 2020, © R. E. Halling)
Boletales – Boletaceae s.l. (26 October 2020, © R. E. Halling) NOTE: 104 genera listed here are conceived in a broad, classical sense (generally the fleshy stipitate mushrooms with pores) including sequestrate morphologies. Phylogenetic inferences from DNA sequences suggest alignment in suborders: Boletineae, Suillineae, Sclerodermatineae, or in the Paxillaceae. Not all genera are well known, equally circumscribed or robustly inferred phylogenetically. Mycorrhizal associations may be confirmed, but many are presumed or suspected. Recent phylogenetic analyses based on DNA sequences infer some true gasteroid (truffle-like, sequestrate) taxa (aside from those in Sclerodermatineae, Suillineae) belong here. Some of the diagnoses are from protologues. Year of publication follows authority (-ies). Afroboletus Pegler & Young (1981) Pileus dry, coarsely fibrillose to squamose, black, often with appendiculate veil remnants, microscopically a trichodermium. Context white, staining red then black. Hymenophore adnexed, white then black, staining red then black. Peronate veil present. Stipe dry, squamose, sometimes annulate, white to gray to black. Spores black, short ellipsoid, longitudinally ridged or winged, sometimes with intercostal veins; a basal thickened rim around sterigmal appendage, lacking a plage. Hymenial cystidia present. Clamp connections absent. Apparently restricted to the African tropics. One sequestrate species known. Ectomycorrhizae presumed with caesalpinoid legumes. Afrocastellanoa M.E. Smith & Orihara (2017) From the protologue: Basidiomata sequestrate, gasteroid, firm, rubbery, with one or a few rhizomorphs at the base. Similar to Octaviania in the morphology of the basidiome and basidiospores, but different from Octaviania in the multilayered peridium and in basidia that are irregularly distributed within the solid gleba, resulting in the absence of a distinct hymenium and subhymenium. -
Aportación De Dos Interesantes Especies De Boletales Al Catálogo
Aportación de dos interesantes especies de boletales al Catálogo Micológico Valenciano Suillellus permagnificus (Pöder) Blanco-Dios y Alessioporus ichnusanus (Alessio, Galli & Littini) Gelardi, Vizzini & Simonini IGNACIO TARAZONA MARTÍNEZ(1) & JOAQUÍN HERRERO CONEJOS(2) (1) C/ Leones 48-15, 46022 Valencia. E-mail: [email protected] (2) C/ Monestir de Poblet 14-15, 46015 Valencia. E-mail: [email protected] Resumen. TARAZONA MARTÍNEZ, I., & HERRERO CONEJOS, J. (2017). Se aportan dos interesantes especies de Boletales: Suillellus permagnificus (Pöder) Blan- co-Dios y Alessioporus ichnusanus (Alessio, Galli & Littini) Gelardi, Vizzini & Si- monini al Catálogo Micológico Valenciano. Butll. Soc. Micol. Valenciana, 22: 73-83. A continuación se describen dos especies de Boletales recolectados en la Co- munidad Valenciana, Suillellus permagnificus (Pöder) Blanco-Dios y Alessiopo- rus ichnusanus (Alessio, Galli & Littini) Gelardi, Vizzini & Simonini. Se aportan datos corológicos e iconografía de estas dos especies. Palabras clave: Boletales, Suillellus, Alessioporus, termófilo, xerófilo, Comuni- dad Valenciana, Parque natural Sierra Calderona. Abstract. TARAZONA MARTÍNEZ, I., & HERRERO CONEJOS, J. (2017). Two interest- ing species of Boletales: Suillellus permagnificus (Pöder) Blanco-Dios and Alessi- oporus ichnusanus (Alessio, Galli & Littini) Gelardi, Vizzini & Simonini are added to the Valencian Mycological Checklist. Butll. Soc. Micol. Valenciana, 22: 73-83. The present work describes two species of Boletales found in the Valencian Community, Suillellus permagnificus (Pöder) Blanco-Dios and Alessioporus ich- nusanus (Alessio, Galli & Littini) Gelardi, Vizzini & Simonini. Chorological and iconographic data are provided for both species. Key words. Boletales, Suillellus, Alessioporus, thermophilic, xerophilic, Valen- cian Community, Sierra Calderona natural park. INTRODUCCIÓN El Parque Natural Sierra Calderona forma parte de una alineación montañosa de orientación NW-SE que separa las cuencas de los ríos Palancia y Turia entre las provincias de Castellón y Valencia. -
Corso Di Aggiornamento Tassonomico Sull'ordine
CORSO DI AGGIORNAMENTO TASSONOMICO SULL’ORDINE BOLETALES IN ITALIA ALLA LUCE DEI NUOVI ORIENTAMENTI FILOGENETICI MOLECOLARI 1a lezione Matteo Gelardi Ordine Boletales E.-J. Gilbert Delimitazione tassonomica • Monofiletico (tutti i taxa appartenenti a questo ordine condividono una singola, comune origine) • Costituito esclusivamente da omobasidiomiceti (basidi unicellulari) • Trama omoiomera • Sistema ifale monomitico, eccezionalmente dimitico o trimitico • Marcata diversità morfologica e imenoforale (non sono presenti forme clavarioidi e coralloidi) • Presenza di particolari composti chimici, soprattutto derivati dell’acido pulvinico (acido variegatico , acido xerocomico, variegatorubina, ecc.) • Modalità nutritiva prevalentemente ectomicorrizica (90% sul totale), altrimenti saprotrofa o mico-parassitica • I generi lignicoli provocano esclusivamente carie bruna, inoltre non sono apparentemente presenti funghi patogeni di piante forestali • I basidiomi sono spesso colonizzati da alcune specie del genere ascomicete parassita Hypomyces (teleomorfo) o Sepedonium (anamorfo), in particolare H. chrysospermus Tulasne & C. Tulasne e taxa affini L’ordine Boletales comprende attualmente 5 subordini, 18 famiglie, oltre 135 generi + 1 genere fossile e circa 1500 specie sinora descritte a livello mondiale! Sistematica ranghi superiori all’ordine Boletales Regno Fungi R.T. Moore Subregno Dikarya Hibbett, T.Y. James & Vilgalys Divisione Basidiomycota R.T. Moore SubDivisione Agaricomycotina Doweld Classe Agaricomycetes Doweld SottoClasse Agaricomycetidae -
Butyriboletus Regius and Butyriboletus Fechtneri: Typification of Two Well-Known Species
CZECH MYCOLOGY 71(1): 1–32, JANUARY 2, 2019 (ONLINE VERSION, ISSN 1805-1421) Butyriboletus regius and Butyriboletus fechtneri: typification of two well-known species 1 2 3 VÁCLAV JANDA *, MARTIN KŘÍŽ ,MIROSLAV KOLAŘÍK 1 Ondříčkova 29, CZ-130 00 Praha 3, Czech Republic; [email protected] 2 National Museum, Mycological Department, Cirkusová 1740, CZ-193 00 Praha 9, Czech Republic; [email protected] 3 Institute of Microbiology of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, CZ-142 20 Praha 4, Czech Republic; [email protected] *corresponding author Janda V., Kříž M., Kolařík M. (2019): Butyriboletus regius and Butyriboletus fechtneri: typification of two well-known species. – Czech Mycol. 71(1): 1–32. The paper deals with type material of two species of the genus Butyriboletus,whichwere described from the Czech Republic, originally as Boletus regius (Krombholz 1983) and Boletus fechtneri (Velenovský 1922). For both species lectotypes are designated, for B. fechtneri also an epitype. The authors present macro- and microscopic descriptions of these species based on the study of rich material including collections from the area of the type localities. Characters distin- guishing both species from similar taxa are discussed. Key words: lectotype, epitype, nomenclature, taxonomy, Czech Republic. Article history: received 21 August 2018, revised 13 November 2018, accepted 16 November 2018, published online 2 January 2019. Janda V., Kříž M., Kolařík M. (2019): Hřib královský – Butyriboletus regius ahřib Fechtnerův – Butyriboletus fechtneri: typifikace dvou dobře známých druhů. – Czech Mycol. 71(1): 1–32. Článek se zabývá typovým materiálem dvou druhů rodu Butyriboletus, původně popsaných z Čes- ké republiky jako hřib královský – Boletus regius (Krombholz 1832) a hřib Fechtnerův – Boletus fecht- neri (Velenovský 1922). -
Assessment of Antifungal Activity of Some Boletes Mushrooms Found in Himalayan Range of Pakistan Against Some Fungi
Pure Appl. Biol., 8(4): 2257-2261, December, 2019 http://dx.doi.org/10.19045/bspab.2019.80171 Research Article Assessment of antifungal activity of some boletes mushrooms found in Himalayan range of Pakistan against some fungi Farwa Batool1, Samina Sarwar1*, Khajista Jabeen1, Tooba Shafiq1 and Abdul Nasir Khalid2 1. Department of Botany, Lahore College for Women University, Lahore-Pakistan 2. Department of Botany, University of the Punjab, Lahore-Pakistan *Corresponding author’s email: [email protected] Citation Farwa Batool, Samina Sarwar, Khajista Jabeen, Tooba Shafiq and Abdul Nasir Khalid. Assessment of antifungal activity of some boletes mushrooms found in Himalayan range of Pakistan against some fungi. Pure and Applied Biology. Vol. 8, Issue 4, pp2257-2261. http://dx.doi.org/10.19045/bspab.2019.80171 Received: 24/05/2019 Revised: 19/07/2019 Accepted: 22/07/2019 Online First: 27/07/2019 Abstract Ectomycorrhizal mushrooms belonging to Boletales (Basidiomycota) viz. Boletus edulis Bull, Hortiboletus rubellus Krombh and Suillus sibiricus Singer were selected to evaluate their antifungal potential against some filamentous fungi viz. Aspergillus terreus Thom, Aspergillus niger Tiegh and Rhizopus stolonifer Vuill. Extracts of these selected mushrooms were prepared in methanol and their various concentrations (0%, 1%, 1.5%, 2% and 3%) were tested against selected pathogenic fungi. B. edulis showed maximum decline (51-53%) in the biomass of R. stolonifer, S. Sibiricus showed 40-45% decline in biomass of A. terreus, while all the concentrations of H. rubellus showed 50-55% decline in the biomass of A. niger. Present work revealed that selected indigenous mushrooms have strong antifungal potential against different pathogenic fungi.