Mycoscience: Advance Publication doi: 10.47371/mycosci.2021.03.001 Short Communication (Received December 26, 2020; Accepted March 8, 2021) J-STAGE Advance Published Date: March 27, 2021 Short Communication Neoboletus infuscatus, a new tropical bolete from Hainan, southern China Shuai Jianga,b, Hong-Xu Mib , Hui-Jing Xiea , Xu Zhanga , Yun Chenb , Zhi-Qun Liangc, Nian-Kai Zenga,* a Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China b Yinggeling Branch of Hainan Tropical Rainforest National Park, Baisha, Hainan 572800, China c College of Science, Hainan University, Haikou 570228, China * Corresponding author. Hainan Medical University, Xueyuan Road, Longhua District, Haikou, China. E-mail address:
[email protected] (N. K. Zeng). Advance Publication - 1 - Mycoscience: Advance Publication ABSTRACT Neoboletus infuscatus (Boletaceae, Boletales) is described as a new species from Yinggeling of Hainan Tropical Rainforest National Park, southern China. It is morphologically characterized by a large basidioma with a nearly glabrous, brownish yellow, yellowish brown to pale brown pileus, pores orangish red when young, yellowish brown to brown when old, context and hymenophore staining blue when injured, a yellow stipe with red punctuations, surfaces of the pileus and the stipe usually covered with a thin layer of white pruina when young. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuclear rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirm that N. infuscatus forms an independent lineage within Neoboletus.