life Article Mitochondrial Genomic Landscape: A Portrait of the Mitochondrial Genome 40 Years after the First Complete Sequence Alessandro Formaggioni, Andrea Luchetti and Federico Plazzi * Department of Biological, Geological and Environmental Sciences, University of Bologna, Via Selmi, 3, 40126 Bologna, BO, Italy;
[email protected] (A.F.);
[email protected] (A.L.) * Correspondence:
[email protected]; Tel.: +39-051-2094-172 Abstract: Notwithstanding the initial claims of general conservation, mitochondrial genomes are a largely heterogeneous set of organellar chromosomes which displays a bewildering diversity in terms of structure, architecture, gene content, and functionality. The mitochondrial genome is typically described as a single chromosome, yet many examples of multipartite genomes have been found (for example, among sponges and diplonemeans); the mitochondrial genome is typically depicted as circular, yet many linear genomes are known (for example, among jellyfish, alveolates, and apicomplexans); the chromosome is normally said to be “small”, yet there is a huge variation between the smallest and the largest known genomes (found, for example, in ctenophores and vascular plants, respectively); even the gene content is highly unconserved, ranging from the 13 oxidative phosphorylation-related enzymatic subunits encoded by animal mitochondria to the wider set of mitochondrial genes found in jakobids. In the present paper, we compile and describe a large database of 27,873 mitochondrial genomes currently available in GenBank, encompassing the whole Citation: Formaggioni, A.; Luchetti, eukaryotic domain. We discuss the major features of mitochondrial molecular diversity, with special A.; Plazzi, F. Mitochondrial Genomic reference to nucleotide composition and compositional biases; moreover, the database is made Landscape: A Portrait of the publicly available for future analyses on the MoZoo Lab GitHub page.