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Linz 2019 ‒ EUSAAT 2019 Proceedings Linz 2019 ‒ EUSAAT 2019 Volume 8, No. 1 ISSN 2194-0479 (2019) ALTEX Proceedings 3D Models & multi-organ- Ethical & legal issues chips (MOC), human-organ-chips (HOC) Free communications 3R Centers in Europe & Implementing international – EU Dir 63/2010 – update national and local centers In silico models 3Rs in education Initiative for and academia implementing serum Advanced safety testing of free culture media cosmetics & consumer In vitro techniques for products and alternatives CNS toxicity and disease to animal testing in studies food safety, nutrition and efficacy Reduction & refinement An integrated Replacement – advanced interdisciplinary approach technologies to animal-free nano- Specific endpoints of material and chemical toxicity safety assessment: Results of the in3 project Stem cell models and technology Biological barriers (hIPS, ES, mES, mIPS…) Disease models using How to account for human cells, uncertainties of reference tissues and organs methods & data? Ecotoxicology Animal experimentation: Efficacy and safety Working towards testing of drugs, a paradigm change medical devices & 'Young Scientists' session biopharmaceutics The world leader in innovative 3D reconstructed human tissue models ISO 9001 CERTIFIED KEY FEATURES OF OUR PRODUCTS TISSUE MODELS AVAILABLE FROM SLOVAKIA AND USA Metabolically active, human cell-derived, 3D reconstructed tissue models EpiDerm™ Objective, quantifiable endpoints Skin Corrosion, Skin Irritation, Phototoxicity, Genotoxicity Excellent in vitro / in vivo correlation Micronucleus and Comet assay, Medical devices, Skin Guaranteed long-term reproducibility Inflammation, Skin metabolism, Radiation Damage, Availability of partial and full thickness tissue models Percutaneous Absorption … Availability of different tissue formats including high throughput screening plate Price convenient testing kits as well as single tissues EpiOcular™ Ocular Irritation, “Sub-Draize” Ocular Irritation Testing (Mild, Milder, Mildest), Specific Ocular Irritation of Surfactants, EXCELLENT CUSTOMER SERVICE Cosmetics and Consumer Products … AND PROFESSIONAL SCIENTIFIC SUPPORT EpiIntestinal™ Prompt reply to customer´s requests and needs Intestinal Toxicity, Drug Delivery, Inflammation, Fibrosis, Infection, Free scientific and technical consulting before purchasing of product Epithelial Restitution … Monitoring of every single shipment Dedicated trainings sessions in our facilities or on-site TISSUE MODELS AVAILABLE FROM USA International support provided by EU, US, Turkey and Japan representatives More than 20 years of tissue quality control data summarized in open databases - the most data of any tissue engineering company ! EpiDermFT™ Since 2016 ISO 9001:2008 certified Anti-Aging, Skin Longevity, Skin Hydration, UV Protection, Percutaneous Absorption … INTERNATIONAL VALIDATIONS AND PROJECTS EpiCorneal™ Drug Delivery, Infection, Inflammation, Ophthalmic Product EpiDerm™ Testing … ECVAM Skin Corrosion, Validated Assay - OECD TG 431 ECVAM Skin Irritation, Validated Assay - OECD TG 439 EpiOral™ and EpiGingival™ ECVAM Pre-Validated and ICH Accepted Phototoxicity Assay Mucosal and Oral Irritation, Transmucosal and Buccal Drug Cosmetics Europe Validation Project on Genotoxicity Assays Delivery, Gum Disease, Oral Cancer, Smokeless Tobacco German Skin Penetration Validation Study for Surfactants and Formulations Effects, Oral Epithelial Proliferation, Antimicrobial Barrier Irritation Potency of Extracts from Medical Devices Study (ISO 10993-10) Function … EpiOcular™ EpiAirway™ ECVAM/Cosmetics Europe Eye Irritation, Validated Assay - OECD TG 492 Drug Delivery, Respiratory Infection and Toxicology, Tobacco US EPA Accepted for Antimicrobial Products with Cleaning Claims (AMCPs) Smoke Toxicology, Nano-particle Toxicology, Gene Expression COLGATE/IIVS Eye Irritation Validation Study Analysis, RNAi/siRNA Therapeutic Drug … Con4Eye Project on Eye Irritation Testing Strategies MelanoDerm™ EpiVaginal™ Skin Lightening-Darkening, Skin Pigmentation Modulation, NIH Funded HIV Research Keratinocyte-Melanocyte Interactions … CONRAD Microbicides Study Psoriasis™ AFFILIATIONS AND MEMBERSHIP IN PROFESSIONAL OR- Anti-Psoriatic Drug Screening, Basic Psoriasis Research ... GANISATIONS AND CONSORTIA IN THE EU EpiVaginal™ Feminine Product Irritation, Microbicide Testing, Immuno- competent, HIV-1 Infection Sexually Transmitted Infection, Inflammation, Vaginal Drug Delivery … Melanoma™ Tumor Invasion, Anti-Melanoma Drug Screening ... PRIMARY CELLS & MEDIA FROM USA Primary human cells derived from human tissue for high throughput screening Your trustworthy partner in the world of in vitro science for the past 30 years MatTek In Vitro Life Science Laboratories MatTek Corporation Mlynské Nivy 73, 821 05 Bratislava, Slovakia, EU 200 Homer Avenue, Ashland, MA 01721, USA Phone: +421-2-3260-7408 Phone: +1-508-881-6771 Fax: +421-2-3260-7404 Fax: +1-508-879-1532 E-mail: [email protected] E-mail: [email protected] Web: www.mattek.com Web: www.mattek.com ABSTRACTS LINZ 2019 Welcome address EUSAAT 2019 Dear friends and colleagues, on behalf of EUSAAT, the European Society for Alternatives to Animal Testing, we welcome you to the 22nd European 3Rs Congress and the 19th EUSAAT Congress in Linz/Austria on October 10-13, 2019. Since 1991 the “Linz-Congress” has emerged in Europe as the major scientific event in the field of the 3Rs and in 2019 the EUSAAT 2019 3Rs Congress Linz will be the largest international 3Rs congress. We are particularly pleased that our international partners from Japan, JSAAE, the Japanese Society for Alternatives to Animal Experiment, and from China, CCARE, the Chinese Center for Alternatives Research and Evaluation, will report on their activities in our sessions on “3R Centers in Europe & international”. EUSAAT 2019 is hosting oral and poster presentations to facilitate discussions and the exchange of ideas to actively promote the most advanced non-animal methods in the life sciences. The Scientific Committee has identified the subjects that are currently in the focus of 3Rs research in Europe as well as internationally. In 2019 the EUSAAT Congress features sessions on Refinement and Reduction (e.g. animal welfare of experimental animals, academia and education, ethical and legal issues, drug development and toxicity studies) as well as on Replacement (e.g. disease models based on human cells and tissues, non-animal tools for basic biomedical research, stem cells, bioprinting, ’omics technologies, human 3D tissue culture methods and human multi-organ-chips (MOCs)). Since at EUSAAT 2018 the sessions on establishing 3Rs networks in Europe were most popular, we will have several sessions on this topic as well as a Round Table Discussion. For the first time, we are holding the YOU EUSAAT 2019 events aimed at young and early career scientists (up to the age of 35) working or planning to work in the 3Rs field. We want to encourage the dialogue among young scientists and with experienced mentors from the 3Rs area to facilitate opportunities for establishing professional networks. In particular, YOU EUSAAT 2019 will provide a free and open atmosphere for exchanging ideas and career experiences and networking with each other! We are proud that four distinguished colleagues have accepted our invitation to serve as keynote speakers. In addition, we are honored that both the Bjorn Ekwall Memorial Fund (BEMF) will hold the 2019 BEMF Award Ceremony and Lecture at EUSAAT 2019 and also that the editor of ALTEX will present the award for the best article published in the ALTEX journal in 2018 at EUSAAT 2019. This year we will again host two Round Table Discussions on topics that are of importance for implementing the 3Rs at the international level. The first will cover the “International Impact of Closing Research Animal Facilities in Europe” and the second will be on “Establishing an International 3Rs Centers Network”. EUSAAT is very happy to continue the “Young Scientists Travel Award (YSTA)” program in 2019 and we want to thank the SET Foundation for its generous funding. The YSTA program will provide 20 young scientists with the opportunity to share their ideas on the implementation of the 3Rs in education and novel test systems to reduce the suffering as well as the number of animals in research. The EUSAAT Board is pleased that the number of sponsors of the EUSAAT congresses has continuously increased, since due to their financial support we can keep the scientific standard high and the congress fee low. This is important to attract young scientists from Europe and abroad. Thus, on behalf of the participants, the EUSAAT Board and the Scientific Committee are grateful to our sponsors. Moreover, we are also pleased that the abstracts of the EUSAAT 2019 are again published in ALTEX Proceedings and we thank Sonja von Aulock, the editor and CEO of ALTEX Edition, for her generous support. Finally, we want to thank our colleagues on the EUSAAT Board and the Scientific Committee for their continuous support in planning the EUSAAT 2019 congress. Horst Spielmann Winfried Neuhaus Dagmar Jirova Dominik Rünzler Secretary General President Vice-President Vice-President ALTEX Proceedings 8, 1/2019 I ABSTRACTS LINZ 2019 List of Abstracts ID Title Authors Page 294 Microphysiological flux balance platform unravels Avner Ehrlich, Konstantinos Ioannidis, Muneef 47 the dynamics of drug induced steatosis Ayyash, Anne Riu, Reine Note, Gladys Ouedraogo, Jan Vanfleteren, Merav Cohen and Yaakov Nahmias 295 Uric acid metabolism and excretion of translucent
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