PRPF19 Antibody Cat. No.: 29-843

PRPF19 Antibody

Antibody used in WB on mouse Antibody used in WB on Human Jurkat 2.5 ug/ml. brain at 1:1000 (Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus ).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Dog, Human, Mouse, Rat, Zebrafish

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PRPF19.

TESTED APPLICATIONS: ELISA, IHC, WB

PRPF19 antibody can be used for detection of PRPF19 by ELISA at 1:1562500. PRPF19 APPLICATIONS: antibody can be used for detection of PRPF19 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 55 kDa WEIGHT:

September 23, 2021 1 https://www.prosci-inc.com/prpf19-antibody-29-843.html Properties

PURIFICATION: Antibody is purified by A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: PRPF19 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: PRPF19

ALTERNATE NAMES: PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV

ACCESSION NO.: NP_055317

PROTEIN GI NO.: 7657381

GENE ID: 27339

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome BACKGROUND: stability. It also may have E3 activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.

REFERENCES: 1) Voglauer, R., (2006) Exp. Cell Res. 312 (6), 746-759.

ANTIBODIES FOR RESEARCH USE ONLY.

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