PRPF19 Antibody Cat. No.: 29-843 PRPF19 Antibody Antibody used in WB on mouse Antibody used in WB on Human Jurkat 2.5 ug/ml. brain at 1:1000 (Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus ). Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Human, Mouse, Rat, Zebrafish Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PRPF19. TESTED APPLICATIONS: ELISA, IHC, WB PRPF19 antibody can be used for detection of PRPF19 by ELISA at 1:1562500. PRPF19 APPLICATIONS: antibody can be used for detection of PRPF19 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 55 kDa WEIGHT: September 23, 2021 1 https://www.prosci-inc.com/prpf19-antibody-29-843.html Properties PURIFICATION: Antibody is purified by protein A chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: PRPF19 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: PRPF19 ALTERNATE NAMES: PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV ACCESSION NO.: NP_055317 PROTEIN GI NO.: 7657381 GENE ID: 27339 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome BACKGROUND: stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair. REFERENCES: 1) Voglauer, R., (2006) Exp. Cell Res. 312 (6), 746-759. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 23, 2021 2 https://www.prosci-inc.com/prpf19-antibody-29-843.html.