ARTICLE DOI: 10.1038/s41467-017-01188-x OPEN Genome-wide prediction of DNase I hypersensitivity using gene expression Weiqiang Zhou 1, Ben Sherwood1,3, Zhicheng Ji1, Yingchao Xue2, Fang Du1, Jiawei Bai1, Mingyao Ying2 & Hongkai Ji 1 We evaluate the feasibility of using a biological sample’s transcriptome to predict its genome- wide regulatory element activities measured by DNase I hypersensitivity (DH). We develop BIRD, Big Data Regression for predicting DH, to handle this high-dimensional problem. Applying BIRD to the Encyclopedia of DNA Elements (ENCODE) data, we found that to a large extent gene expression predicts DH, and information useful for prediction is contained in the whole transcriptome rather than limited to a regulatory element’s neighboring genes. We show applications of BIRD-predicted DH in predicting transcription factor-binding sites (TFBSs), turning publicly available gene expression samples in Gene Expression Omnibus (GEO) into a regulome database, predicting differential regulatory element activities, and facilitating regulome data analyses by serving as pseudo-replicates. Besides improving our understanding of the regulome–transcriptome relationship, this study suggests that transcriptome-based prediction can provide a useful new approach for regulome mapping. 1 Department of Biostatistics, Johns Hopkins University Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA. 2 Department of Neurology, Hugo W. Moser Research Institute at Kennedy Krieger and Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. 3Present address: School of Business, University of Kansas, 1654 Naismith Drive, Lawrence, KS 66045, USA. Correspondence and requests for materials should be addressed to H.J. (email: [email protected]) NATURE COMMUNICATIONS | 8: 1038 | DOI: 10.1038/s41467-017-01188-x | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/s41467-017-01188-x fundamental question in functional genomics is how data analyses. It can also be used to predict differential regulatory Agenes’ activities are controlled temporally and spatially. element activities such as changes of chromatin accessibility To answer this question, it is crucial to comprehensively between different cell types or differentiation time points. map activities of all genomic regulatory elements (i.e., regulome) and understand the complex interplay between the regulome and transcriptome (i.e., transcriptional activities of all genes). Reg- Results ulome mapping has been accelerated by high-throughput tech- Big data regression for predicting DH. Predicting DH using nologies such as chromatin immunoprecipitation coupled with gene expression can be formulated as a problem of building high-throughput sequencing (ChIP-seq)1 and sequencing of millions of regression models, one per genomic locus, to describe chromatin accessibility (e.g., DNase-seq2 for DNase I hypersen- the relationship between the DH level at that locus (response) and sitivity (DH), FAIRE-seq3 for formaldehyde-assisted isolation of the expression levels of tens of thousands of genes (predictors). regulatory elements, and ATAC-seq4 for assaying transposase- For method development and evaluation, we compiled DNase- accessible chromatin). So far, these technologies have only been seq and exon array (i.e., gene expression) data for 57 distinct applied to interrogate a small subset of all possible biological human cell types with normal karyotype from ENCODE (Sup- contexts defined by different combinations of cell or tissue type, plementary Data 1). They were randomly partitioned into a disease state, time, environmental stimuli, and other factors. A training dataset (40 cell types) and a test dataset (17 cell types). major limitation of these technologies is the difficulty in simul- After filtering out genomic regions with weak or no DH signal taneously analyzing a large number of different biological con- across all 40 training cell types, 912,886 genomic loci (referred to texts. This limitation along with practical constraints such as lack as “DNase I hypersensitive sites” or “DHSs” hereinafter) with of materials, antibodies, resources, or expertise has hindered their unambiguous DNase-seq signal in at least one training cell type application by the vast majority of biomedical investigators from were retained for subsequent analyses (“Methods”). small laboratories. For each locus, prediction models were constructed using the Numerous researchers have examined how genes’ transcrip- 40 training cell types. Prediction performance was evaluated using tional activities can be predicted using activities of their asso- the 17 test cell types based on three types of statistics (Fig. 2a, – ciated regulatory elements5 7. However, the interplay between “Methods”). First, we evaluated a method’s ability to predict the regulome and transcriptome is bidirectional due to feedback8, 9.A variation of DH levels across different genomic loci by computing systematic understanding of this relationship in the reverse the Pearson’s correlation between the predicted and true DH direction, to what extent regulatory elements’ activities can be values (or P–T correlation) across all DHSs within the same cell ’ predicted by transcriptome, is still lacking. type (cross-locus correlation rL). Second, we evaluated a method s Here we investigate the problem of using gene expression to ability to predict the relative activities of the same DHS in predict DH based on the data generated by the Encyclopedia of different cell types by computing the P–T correlation across DNA Elements (ENCODE) Project10. Besides creating a more different cell types at each genomic locus (cross-cell-type – complete picture of the regulome transcriptome relationship, this correlation rC). Third, we computed the total squared prediction investigation also has important practical implications for reg- error normalized by the total DH data variance (τ) to characterize ulome mapping. Gene expression is the most widely measured the proportion of data variation not explained by the prediction. data type in high-throughput functional genomics. Measuring The regression for each locus can be constructed using either expression does not require large amounts of materials and its neighboring genes or all genes as predictors (Supplementary complex protocols, and technologies for expression profiling are Fig. 1). We tested both strategies (see “Methods” and Supple- relatively mature. Thus, expression data are routinely collected mentary Figs. 2–6 for details). The latter strategy requires one to when other functional genomic data types are difficult to generate deal with a challenging big data regression problem, which due to technical or resource constraints. Today, the Gene involves fitting ~1 million high-dimensional regression models, Expression Omnibus (GEO) database11 contains 200,000+ each with a large number (18,000+) of predictors and small human gene expression samples from a broad spectrum of bio- sample size. To cope with the high dimensionality and heavy logical contexts, as compared to only ~7000 human ChIP-seq, computation, we developed the BIRD algorithm. BIRD predicts DNase-seq, FAIRE-seq, and ATAC-seq samples. If one can use DH at each genomic locus by combining predictions from two the ENCODE data to build prediction models and apply them to types of models, locus-level model and pathway-level model, existing and new transcriptome data to predict regulome, the through model aggregation (Fig. 1b). ÀÁ catalog of biological contexts with regulome information may be The locus-level model, denoted by BIR X; Y , groups corre- quickly expanded (Fig. 1a). This will provide a useful approach lated predictors (i.e., co-expressed genes) into clusters and for regulome mapping that is complementary to existing transforms each cluster into one predictor. A small subset of experimental methods. It will also allow researchers to more the transformed predictors informative for prediction is then effectively use expression data to study gene regulation. Unlike a selected for each locus to construct a regression model recent study that imputes one functional genomic data type based (“Methods”). Clustering reduces the predictor dimension, on multiple other data types, which are non-trivial to collect12, mitigates co-linearity, and makes the predictors less sensitive to prediction in this study is based on one single but widely available measurement noise13. Compared to using individual genes as data type and hence can have a substantially broader range of predictors, clustering improved the prediction accuracy (“Meth- applications. During our investigation, we develop a big data ods”, Supplementary Fig. 4). Conceptually, constructing regres- regression approach, BIRD (Fig. 1b), to handle the prediction sion models using selected gene clusters is a group variable problem where both predictors (i.e., transcriptome) and respon- selection approachÀÁ applied in a prediction setting14. However, ses (i.e., regulome) are ultra-high-dimensional, which is an unlike BIR X; Y , conventional group variable selection methods emerging problem in the analysis of big data. We show that BIRD are primarily developed for modeling a univariate response using – can provide practically useful predictions of chromatin accessi- high-dimensional predictors15 17. They pay less attention to bility using gene expression. BIRD-predicted DH can be used to important issues such as computational efficiency and robustness predict