DOCSLIB.ORG

Pasteurella Multocida Abcess in an African Patient with Human

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Pasteurella Multocida Abcess in an African Patient with Human 700 Clinical Microbiology and Infection, Volume 3 Number 6, December 1997 rhabdomyolysis is doubtful in these cases [6]. In our contact. The gastrointestinal tract is also a possible port patient there was no seizure, and ketoprofen has not of entry, since cases of pasteurella peritonitis after been associated with muscle damage, so that GBS is the endoscopy have been described [2]. Underlying disease only factor that can explain the rhabdomyolysis. The and immunocompromised status increase the sus- pathogenesis is not clear, but it has been suggested that ceptibility to infection [2,3,6,7]. In humans, clinical infection may lead to the production of a proteolytic manifestations of pasteurella infection can be roughly factor or a polypeptide which causes muscle degrada- divided into three groups [2]: a local cellulitis with tion [6]. or without deep-seated infections [4,5l, a pulmonary infection [1,3,8] or bacteremia with or without meta- D. Barcatl,J Constans', static lesions [2,6,9]. S. Sire', J. M. Ragnaud' Clinique Mkhcale et des Maladm Infectieuses, Case report H6pital Pellegrin, A 25-year-old Liberian woman had been living in Bordeaux, France; Belgium since January 1995. In 1992 and 1993 she had 2Service de Mkdecine Interne terminations of pregnancy. In July 1995 she was found et Pathologie Vasculaire, to be HIV seropositive when she presented with a H8pital Saint-Andri., breast abscess. At that time her CD4 lymphocyte count Bordeaux, France was 184 cells/pL. In August 1995 she was admitted to the Antwerp University Hospital because of high fever Acccepted 16 July 1997 (39 "C), abdominal pain, diarrhea, vomiting and cough. On clinical examination she was found to have general- References ized lymphadenopathy and hepatosplenomegaly. An 1. Singh U, Scheld WM. Infectious etiologies of rhabdo- abdominal CT scan confirmed the hepatosplenomegaly myolysis; three case reports and review. Clin Infect Dis 1996; and showed an omental 'cake' with adhesions and 22: 642-9. a multi-chambered abscess surrounded by micro- 2. Murioz P, Coque T, Rodriguez Creixems M, Bernaldo de Quirbs JCL, Moreno S, Bouza E. Group B Streptococcus: abscesses in the pouch of Douglas, bilateral pelvic a cause ofurinary tract infection in non pregnant adults. Clin inflammatory disease with salpingitis and a mild right Infect Dis 1992; 14: 492-6. hydronephrosis. The abscess was drained externally 3. Dunne DW, Quagliarello V Group B streptococcal menin- following insertions under CT guidance of a catheter. gitis in adults. Mehcine 1993; 72: 1-10, Pasteurella multocida was cultured from the drained 4. Sarniiento R, Wilson FM, Khatib R. Group B streptococcal hernopurulent fluid. Laboratory results showed anemia meninstis in adults: case report and review of the literature. and abnormal liver function tests. Blood. urine and Scand J Infect Dis 1993; 25: 1-6. fecal cultures were negative. 5. Mason S, Lewno MJ, Schutze GE. Clinical usefulness of The patient was treated with intravenous cipro- cerebrospinal fluid bacterial antigen stuhes. J Pedmtr 1994; floxacin 200 mg twice daily for 7 days, followed by oral 125: 235-8. ciprofloxacin 250 mg twice daily for 9 days, and then 6. Turner MC, Naumburg EG. Acute renal failure in the neonate, Two fatal cases due to groq B ,'jtrepto,cocti with arnoxicillin/clavulanic acid 500 mg/125 mg four times rhabdomvolvsis. Clin Pematr (Phila) 1987; 26: 189--90. a day. After 1 month she had responded well to treatment but the CT-scan appearances remained unchanged. The patient was discharged and sub- sequently lost to follow-up. Pasteurella mulfocida abcess in an African patient with To our knowledge, this is the first description of an human immunodeficiency virus infection HIV-seropositive patient developing an abdominal abscess with Pastewella multocida. Very few cases of Clin Micvobiol Infect 1997; 3: 700-701 pasteurella infection in HIV-seropositive patients have We report here a case of abdominal Pastewella multocida been described. Most of them were cases of peritonitis, abscess in an African HIV-positive patient. Pasteurella pneumonia and sepsis [3,9,10]. It is unclear how our m~ltocidais a Gram-negative coccobacillus which can patient became infected. There was no history of be found in the normal flora of the nasopharynx animal bite or scratch or exposure to animal secretions. and gastrointestinal tract of both domestic and wild She never underwent an endoscopic procedure. mammals and birds [ 11. Human transmission generally Pasteurella infections respond well to benzylpenicikn, occurs via animal bites and scratches and via exposure second- and third-generation cephalosporins, tetra- to animal secretions [2-51. In 5-15% of Pastewella cyclines, chloramphenicol and quinolones. The sus- multocida infections there is no evidence of animal ceptibility to erythromycin is variable and there is Short Communications and Letters to the Editors 701 universal resistance to vancomycin and clindamycin infrastructure was completely destroyed. Several pro- [2,4,111. grams are being developed aimed at improving medical practice as well as at more general educational objec- K. Depaetere 'r2, R. Colebunders'J2, L. MIS', tives. Our university has recently been involved in A. S. Pym3, M. Ieven2 training courses for medical doctors, given at the 'Institute of Tropical Mehcine, Faculty of Medicine at the University of Phnom Penh. Antwerp, Belgium; The local doctors, 16 in total, differed in age and in 'University Hospital Antwerp, Belgium; their hsciplines (surgeons and non-surgeons) and also 3St Mary's Hospital, London, UK had their practices in different areas of the country. They participated very actively and enthusiastically in the update programs which were organized for several References disciplines. 1. Hubbert WR, Rosen MN. Pasteurella multocida infection in During the microbiology courses it became man unrelated to animal bite. An1 J Public Health 1970; 60: apparent that many infectious diseases are virtually 1109-17. unknown to the doctors. They were almost unaware 2. Rafi F, Barrier J, Baron D, Drugeon HB, Nicolas F, of the problem of schistosomiasis, a parasitic disease Courtieu AL. Pasteurdla multocida bacteremia: report of that must be very frequent in a country living mainly thirteen cases over twelve years and review of the literature. off freshwater fish and rice production. Melioidosis, Scand J Infect Dis 1987; 19: 385-93. endemic in Cambodia and the surrounding countries 3. Drabick JJ, Gasser RA Jr, Saunders NB et al. Parteunlla multocida pneumonia in a man with AIDS and nontraumatic of Vietnam and Thailand, was also unknown to the feline exposure. Chest 1993; 103: 7-11. participants. 4. Brue C, Chosidow 0. Parfeuvella multocida wound infection Furthermore, it appears that many serious infections and cellulitis. Int J Dermatol 1994; 3(7): 471-2. are diagnosed as typhoid fever, and where neurologic 5. Chraibi A, Dang PM, Bedane C, Bonnetblanc JM. Pasteurella involvement occurs this is considered as a possible multocida et ulcCration de jambe. Ann Dermatol Veneorol case of Japanese encephalitis B. The question of how 1994; 121: 399-401. a Pseudomonas aeruginosa infection should be treated 6. Genne D, Siegrist HH, Monnier P, Nobel M, Humair L, de remained unanswered, since nobody ever diagnoses this Torrente A. Pusteurella multocida endocarditis: report of a case infection. and review of the literature. Scand J Infect Dis 1996; 28: The major reason for this lack of medical know- 95-7. 7. Caldera L, Dutschriian L, Carmo G, Sousa G. Fatal Pusterir~llu ledge may be the absence of diagnostic microbiological multocida infection in a systenlic lupus erytheniatosus patient. laboratories in the country: none of the 16 parti- Infection 1993; 21(4): 254-5. cipating doctors had ever taken a sample for ~rllcrobial 8. Ruiz-Santana S, Antunez A, Arnas M, Rodriguez de Castro culture or serologic analysis during his entire career. F, Manzano JH. Telescoping plugged catheter: an unusual Detection of hepatitis B and HIV infection in donor way of diagnosing Parteurella mtrltocida pneumonia. Chest blood and microscopic detection of acid-fast bacilli in 1991; 6: 1517. sputum samples were the only exceptions. The absence 9. Baker D, Stahlnian GC. Pasteurellu multocida infection in a of laboratories also results in the inability to perform patient with AIDS. J Tenn Med Assoc 1991; 84: 325-6. susceptibility testing, which leads to inappropriate 10. Elsey RM, Carson RW, DuBose TD Jr. Pustetrrellu multocida antibiotic therapy. This problem was illustrated during peritonitis in an HIV-positive patient on continuous cycling the practical exercises organized for the microbiology peritoneal dialysis. hiJ Nephrol 1991; 1: 61-3. 11. Von Schroeder HP, Bell RS. Pastewella multocidu osteo- course. myelitis: an unusual case presentation. Can J Infect Dis 1996; Each of the 16 doctors performed a culture of 7: 137-9. his own stool, in which antibiotic-containing paper disks were applied on the McConkey agar niediuni of the primary culture. The susceptibility of colonies of resistant coliform bacteria was analyzed in more detail High levels Of resistance to antibiotics in ESCherkhh CO/i using firby-Bauer antibiograms. The results were sur- isolated from Cambodian doctors during a postgraduate prising: six of the 16 doctors harbored Escherirhin roli course (identified by conventional techniques) resistant to Clin Micvohiol Infect 1997; 3: 701-702 quinolones, two isolates were gentamicin resistant, and three isolates (from two subjects) were also resistant to Chhodia has ~fferedtremendously from its recent cefiriaxone. The resistance rate in this small sample is turbulent history. The country and its People are higher than that found in hospitalized patients in struggling to recover from their wounds. The medical E~~~~~and the USA, It nyay be that in the absence of .
Load more

Recommended publications
  • Redalyc.Establishment of a Pathogenicity Index for One-Day-Old
    Revista Brasileira de Ciência Avícola ISSN: 1516-635X [email protected] Fundação APINCO de Ciência e Tecnologia Avícolas Brasil Pilatti, RM; Furian, TQ; Lima, DA; Finkler, F; Brito, BG; Salle, CTP; Moraes, HLS Establishment of a Pathogenicity Index for One-day-old Broilers to Pasteurella multocida Strains Isolated from Clinical Cases in Poultry and Swine Revista Brasileira de Ciência Avícola, vol. 18, núm. 2, abril-junio, 2016, pp. 255-260 Fundação APINCO de Ciência e Tecnologia Avícolas Campinas, Brasil Available in: http://www.redalyc.org/articulo.oa?id=179746750008 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Brazilian Journal of Poultry Science Revista Brasileira de Ciência Avícola Establishment of a Pathogenicity Index for One-day- ISSN 1516-635X May - Jun 2016 / v.18 / n.2 / 255-260 old Broilers to Pasteurella multocida Strains Isolated http://dx.doi.org/10.1590/1806-9061-2015-0089 from Clinical Cases in Poultry and Swine Author(s) ABSTracT Pilatti RMI Although Pasteurella multocida is a member of the respiratory Furian TQI microbiota, under some circumstances, it is a primary agent of diseases Lima DAI , such as fowl cholera (FC), that cause significant economic losses. Finkler FII Brito BGII Experimental inoculations can be employed to evaluate the pathogenicity Salle CTPI of strains, but the results are usually subjective and knowledge on the Moraes HLSI pathogenesis of this agent is still limited.
    [Show full text]
  • ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization Of
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by KhartoumSpace ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﻴﻢ Molecular Characterization of Pasteurella multocida Vaccine Strains By: Hajir Badawi Mohammed Ahmed B.V.M Khartoum University (2006) Supervisor: Dr. Awad A. Ibrahim A dissertation submitted to the University of Khartoum in partial fulfillment of the requirements for the degree of M. Sc. in Microbiology Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum June, 2010 Dedication To my mother Father Brother, sister and friends With great love Acknowledgments First and foremost, I would like to thank my Merciful Allah, the most beneficent for giving me strength and health to accomplish this work. Then I would like to deeply thank my supervisor Dr. Awad A. Ibrahim for his advice, continuous encouragement and patience throughout the period of this work. My gratitude is also extended to prof. Mawia M. Mukhtar and for Dr. Manal Gamal El-dein, Institute of Endemic Disease. My thanks extend to members of Department of Microbiology Faculty of Veterinary Medicine for unlimited assistant and for staff of Central Laboratory Soba. I am grateful to my family for their continuous support and standing beside me all times. My thanks also extended to all whom I didn’t mention by name and to the forbearance of my friends, and colleagues who helped me. Finally I am indebted to all those who helped me so much to make this work a success. Abstract The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level.
    [Show full text]
  • Pasteurella Multocida Isolated from Cattle
    Journal of Applied Pharmaceutical Science Vol. 3 (04), pp. 106-110, April, 2013 Available online at http://www.japsonline.com DOI: 10.7324/JAPS.2013.3419 ISSN 2231-3354 Antibiotic Susceptibility and Molecular Analysis of Bacterial Pathogen Pasteurella Multocida Isolated from Cattle Azmat Jabeen, Mahrukh Khattak, Shahzad Munir*, Qaiser Jamal, Mubashir Hussain Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, Pakistan. ARTICLE INFO ABSTRACT Article history: Pasteurella multocida is a Gram negative, non motile and coccobacillus bacterium. It has 5 strains i.e. A, B, D, Received on: 01/02/2013 E and F and 16 serotypes (1-16). In present study, we analyzed Pasteurella multocida B: 2 strains, responsible Revised on: 19/02/2013 for Hemorrhagic Septicemia (HS) in cattle, on morphological/microbial, biochemical, molecular level and to Accepted on: 15/03/2013 check the antibiotic sensitivity of the Pasteurella multocida. Microbial analysis showed that while grown on Available online: 27/04/2013 Brain Heart Infusion agar plates and Blood Agar Base Medium, grayish lustrous colonies of Pasteurella multocida were observed. Gram staining showed that Pasteurella multocida are gram negative. Microscopic Key words: observations revealed it to be coccobacillus and it was non- motile. Identification was conducted by Pasteurella multocida, conventional biochemical tests and percentage identification of Analytical Profile Index was 96 %. Antibiotic Hemorrhagic Septicemia, sensitivity with different antibiotics was checked by disk diffusion method and was found resistant to Analytical Profile Index, Augmentin, Amoxicillin and Aztreonam and was more susceptible to Ceftiofur. On molecular level its DNA Antibiotic sensitivity. was extracted and was run with marker having range from 0.5 – 10 kb.
    [Show full text]
  • Clinical Antibiotic Guidelines†
    CLINICAL ANTIBIOTIC GUIDELINES† ACYCLOVIR IV*/PO *RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Herpes Simplex Cytomegalovirus Epstein Barr Virus Herpes Zoster Indicated: IV: 1. Therapy for suspected or documented Herpes simplex encephalitis 2. Therapy for suspected or documented Herpes simplex infection of a newborn or immunocompromised patient 3. Therapy for primary varicella infection in immunocompromised patients 4. Therapy for severe or disseminated varicella-zoster infections in immunocompromised or immunocompetent patient 5. Therapy for primary genital herpes with neurologic complications Oral: 1. Therapy for primary Herpes simplex infections (oral/genital) 2. Suppressive (preventative) therapy for recurrent (³ 6 episodes/year) severe Herpes simplex infections (oral/genital) 3. Episodic therapy for recurrent (³ 6 episodes/year) Herpes simplex genital infections (initiate within 24 hours of prodrome onset) 4. Prophylaxis for HSV in bone marrow transplants where patient is seropositive 5. Therapy and suppressive therapy for Eczema Herpeticum 6. Therapy for varicella-zoster infections in immunocompetent and immunocompromised patients (if not severe) 7. Therapy for primary varicella infections in pregnancy 8. Therapy for varicella in immunocompetent patients > 13 years old (initiate within 24 hours of rash onset) 9. Therapy for varicella in patients < 13 years old (initiate within 24 hours of rash onset) if there is a chronic cutaneous or pulmonary disorder, long term salicylate therapy, or short, intermittent or aerosolized corticosteroid use Not Indicated: 1. Therapy for acute Epstein-Barr infections (acute mononucleosis) 2. Therapy for documented CMV infections CLINICAL ANTIBIOTIC GUIDELINES† AMIKACIN RESTRICTED TO ANTIBIOTIC FORM Predictable activity: Unpredictable activity: No activity: Enterobacteriaceae Staphylococcus spp Streptococcus spp Pseudomonas spp Enterococcus spp some Mycobacterium spp Alcaligenes spp Anaerobes Indicated: 1.
    [Show full text]
  • Interaction Study of Pasteurella Multocida with Culturable Aerobic
    Hanchanachai et al. BMC Microbiology (2021) 21:19 https://doi.org/10.1186/s12866-020-02071-4 RESEARCH ARTICLE Open Access Interaction study of Pasteurella multocida with culturable aerobic bacteria isolated from porcine respiratory tracts using coculture in conditioned media Nonzee Hanchanachai1,2, Pramote Chumnanpuen2,3 and Teerasak E-kobon2,4,5* Abstract Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P. multocida and other aerobic commensal bacteria in the porcine respiratory tract is not well understood. This study aimed to determine the interactions between porcine P. multocida capsular serotype A and D strains and other culturable aerobic bacteria isolated from porcine respiratory tracts using a coculture assay in conditioned media followed by calculation of the growth rates and interaction parameters. Results: One hundred and sixteen bacterial samples were isolated from five porcine respiratory tracts, and 93 isolates were identified and phylogenetically classified into fourteen genera based on 16S rRNA sequences. Thirteen isolates from Gram-negative bacterial genera and two isolates from the Gram-positive bacterial genus were selected for coculture with P. multocida. From 17 × 17 (289) interaction pairs, the majority of 220 pairs had negative interactions indicating competition for nutrients and space, while 17 pairs were identified as mild cooperative or positive interactions indicating their coexistence. All conditioned media, except those of Acinetobacter, could inhibit P. multocida growth. Conversely, the conditioned media of P. multocida also inhibited the growth of nine isolates plus themselves.
    [Show full text]
  • Metaproteomics Characterization of the Alphaproteobacteria
    Avian Pathology ISSN: 0307-9457 (Print) 1465-3338 (Online) Journal homepage: https://www.tandfonline.com/loi/cavp20 Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar To cite this article: José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssusgallinae (De Geer, 1778), Avian Pathology, 48:sup1, S52-S59, DOI: 10.1080/03079457.2019.1635679 To link to this article: https://doi.org/10.1080/03079457.2019.1635679 © 2019 The Author(s). Published by Informa View supplementary material UK Limited, trading as Taylor & Francis Group Accepted author version posted online: 03 Submit your article to this journal Jul 2019. Published online: 02 Aug 2019. Article views: 694 View related articles View Crossmark data Citing articles: 3 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=cavp20 AVIAN PATHOLOGY 2019, VOL. 48, NO. S1, S52–S59 https://doi.org/10.1080/03079457.2019.1635679 ORIGINAL ARTICLE Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero a,b, Sandra Díaz-Sanchez a, Olivier Sparagano c, Robert D. Finn d, José de la Fuente a,e and Margarita Villar a aSaBio.
    [Show full text]
  • Multicenter Evaluation Supports Accuracy of the Beckman Coulter
    Multicenter Evaluation Supports Accuracy of the Beckman Coulter Gram-Negative Identification Panel ECCMID 2016 with Improved Database for Clinically Significant Bacteria Hindler J1, Lewinksi, M, Schreckenberger P2, Beck C3, Nothaft D3, Bobolis J3, Carpenter D3, Mann L, Madriaga OM3, Wong T3, Smoot L3 1UCLA David Geffen School of Medicine, Los Angeles, CA, 2Loyola University Medical Center, Maywood, IL, and 3Beckman Coulter Microbiology, West Sacramento, CA INTRODUCTION METHODS (Continued) RESULTS (Continued) Incorrect Results (Table 3) A multicenter study was performed to evaluate the performance of the • MicroScan Dried Gram-negative Identification panels were assessed at Fermenter Results (Table 2) There were 8 incorrect results out of a total of 609 clinical and stock MicroScan Gram-negative Identification (NID) panels’ updated organism 16-18 hours. If any of the following criteria were met, reagents were Fermenters were identified correctly 99.2% (417/420) and only 3 isolates. database and revised performance matrix on the WalkAway system and the added to the VP, IND, NIT, and TDA tests and the results reported: isolates generated an incorrect identification. autoSCAN-4 instrument. • GLU, SUC, or SOR were positive Table 2. Fermenter Total Performance Table 3. Total Isolates Incorrectly Identified • ARG and OF/G were positive Reference Identification NID Panel Identification The Gram-negative organism database was revised and includes an • ARG and CET were positive B. cepacia complex 85.92% additional 39 new taxa – 22 fermentative and 17 non-fermentative • LYS or ORN were positive Achromobacter species R. pickettii 10.23% Correct ID (with organisms – along with updated nomenclature. A multicenter study was Correct ID Incorrect ID B.
    [Show full text]
  • Pasteurellosis in Backyard Poultry and Other Birds
    Pasteurellosis in backyard poultry and other birds Shane R. Raidal1 Case 1 An investigation was requested to determine the cause of sudden mortalities in a group of 3 week old goslings bought from a local market. Within 4 days of purchase 2 of 12 birds were found very weak. Both died within 12 hours. One comatose and 2 dead goslings were submitted for necropsy examination. They were in good physical condition. Haematology results on blood collected from the live bird demonstrated a marked leucocytosis (37.3 × 109 cells/mL) due to a heterophilia (30.4 × 109 heterophils/mL) with a left shift. Necropsy examination demonstrated multifocal abscess throughout the lungs, mild hepatomegaly and a mild thickening of the airsacs. Histological examination demonstrated a severe multifocal necrosuppurative airsacculitis and pneumonia with pulmonary abscessation. A heavy growth of Pasteurella multocida was cultured from the lungs and liver. Further cases did not occur in remaining birds which were treated with doxycycline (5 g/L drinking water). Case 2 A backyard poultry flock of 29 Rhode Island red chickens consisting of 27 hens and 2 roosters all 1 year of age with a 3 month history of illness and deaths was investigated. Affected birds reportedly developed swollen eyes, lethargy, and inappetance and death within 2 days of the onset of clinical signs. One affected hen was submitted for necropsy. Haematology results were normal. Necropsy demonstrated pale, swollen periorbital sinuses, nares and beak and a caseous exudate from the choana. The larynx, trachea, lungs, airsacs and other visceral organs appeared normal. A moderate burden of Heterakis gallinarum was present in the caecae.
    [Show full text]
  • Evaluation of Different API Systems for Identification of Porcine Pasteurella Multocida Isolates
    ARTICLE IN PRESS Available online at www.sciencedirect.com Research in Veterinary Science xxx (2008) xxx–xxx www.elsevier.com/locate/rvsc Evaluation of different API systems for identification of porcine Pasteurella multocida isolates Y.A. Vera Lizarazo, E.F. Rodrı´guez Ferri, C.B. Gutie´rrez Martı´n * Department of Animal Health, Section of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Leo´n, 24007 Leo´n, Spain Accepted 5 February 2008 Abstract An exhaustive biochemical characterisation of 60 porcine Pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by PCR and all belonging to the capsular serogroup A, was performed by means of four commercial systems. The API 20NE correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the API 20E method. The high diversity exhibited by the API 50CHB/E system, with six different patterns, does not advise its use as additional system for a definitive identification at the species level, but this method could be a potential tool for characterising P. multocida isolates below this level. The more uniform reactions yielded by the API ZYM test make this system helpful in the confirmatory identification of this organism. The high variability (20 profiles) obtained when the four systems are taken together also suggests their usefulness for epide- miological purposes in order to sub-type P. multocida isolates. Ó 2008 Elsevier Ltd. All rights reserved. Keywords: Pasteurella multocida; Swine; Biochemical characterisation; Identification Although Pasteurella multocida is part of the commensal In this study, we report an exhaustive biochemical char- flora of the upper respiratory tract of swine, it may induce acterisation of P.
    [Show full text]
  • Pasteurella Multocida Bacteremia and Septic Arthritis in a Patient with Non-Bite Animal Exposure
    Open Access Case Report DOI: 10.7759/cureus.14162 Pasteurella Multocida Bacteremia and Septic Arthritis in a Patient With Non-Bite Animal Exposure Lex P. Leonhardt 1 , Aamir Pervez 1 , Wesley Tang 1 1. Internal Medicine Residency, Kettering Medical Center, Dayton, USA Corresponding author: Lex P. Leonhardt, [email protected] Abstract Pasteurella multocida (P. multocida) is a gram-negative, facultative anaerobe, and it is most commonly found in the oropharynx of healthy domestic animals, especially cats and dogs. P. multocida is classified as an opportunistic pathogen, infecting individuals only through direct contact via local trauma caused by animal bites or scratches. More serious infections, while rare, are typically associated with immunocompromised states, known pre-existing cavitary lung pathology, or malignancy. In this report, we present a case of P. multocida infection without known trauma, which had likely spread via respiratory droplets causing bacteremia, septic shock, and subsequent septic arthritis of the left knee. Categories: Internal Medicine, Infectious Disease, Orthopedics Keywords: diabetic foot ulcers, pasteurella multocida, bacteremia, septic shock, septic arthritis, chronic wounds, diabetic foot ulcers management Introduction Pasteurella multocida (P. multocida) is a ubiquitous gram-negative, facultative anaerobe, which is commonly found in the oropharynx of cats and dogs. In general, most human infections are the result of zoonotic transmission via cat/dog bites and/or scratches. Consequently, most infections where P. multocida has been isolated primarily involve the skin or soft tissue. Despite penetrating injury being the most common mechanism of infection, serious infections such as bacteremia, peritonitis, and meningitis are exceedingly rare. In more serious infections, the host typically has certain medical conditions such as pre-existing cavitary lung pathology, immunocompromised state, malignancy, or dialysis dependence.
    [Show full text]
  • Pasteurella Multocida
    technical sheet Pasteurella multocida Classification or chronic. A survey of clinical signs associated Small, Gram-negative rod, bipolar staining with pasteurellosis in laboratory rabbits showed rhinitis, conjunctivitis, abscesses, and otitis media Family as the most common presenting signs. Septicaemia, bronchopneumonia, genital infection, arthritis, Pasteurellaceae osteomyelitis, and dacryoadenitis are also possible, as are infections of skin wounds, such as catheter tracts. Part of a larger group of bacteria, the Pasteurella- Haemophilus-Actinobacillus complex. The taxonomy of The typical presentation of a P. multocida infection in a this group is complex and incomplete. Additionally, the rabbit is a catarrhal or mucopurulent nasal exudate. The members are not all readily speciated by biochemical exudate may not be visible at the external nares, and means. in many cases, matted fur around the nares and on the front paws are the only signs noted. Lesions found in Affected species target organs noted above can be generally categorized Most mammals can carry P. multocida. Of laboratory as suppurative at necropsy. animals, rabbits are the most severely clinically affected. This organism can infect humans. Diagnosis P. multocida may be diagnosed via culture, PCR, Frequency or serology. The nasopharyx is difficult to sample Uncommon in well-managed rabbit colonies, although in conscious rabbits, and carrier animals may have sporadic outbreaks occur; carriage is common in pet negative culture results, due to carriage of the organism rabbits, dogs, cats, and livestock. P. multocida is rare in in the middle ear or the paranasal sinuses. Serology laboratory rats and mice. In our experience, occasional is available, but does not diagnose active infection.
    [Show full text]
  • Haemophilus-Pasteurella Group
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Jan. 1992, p. 12-18 Vol. 42, No. 1 0020-7713/921010012-07$02.OO/O Copyright 0 1992, International Union of Microbiological Societies Application of Multivariate Analyses of Enzymic Data to Classification of Members of the Actinobacillus- Haemophilus-Pasteurella Group VESLEMJbY MYHRVOLD,' ILIA BRONDZ,2t AND INGAR OLSEN'" Department of Microbiology, Dental Faculty, University of Oslo, Oslo, Norway,' and Research Department, National Institute of Occupational Health, UmeB, Sweden2 Outer membrane vesicles and fragments from Actinobacillus actinomycetemcomitans, Actinobacillus lignier- esii, Actinobacillus ureae, Haemophilus aphrophilus, Haemophilus paraphrophilus, Haemophilus influenzae, Haemophilus parainfluenzae, Pasteurella haemolytica, and Pasteurella multocida were isolated and examined semiquantitatively for 19 enzyme activities by using the API ZYM micromethod. The enzyme contents of vesicles and fragments were compared with the enzyme contents of whole cells of the same organisms. Enzymic data were analyzed by using principal-componentanalysis and soft independent modeling of class analogy. This technique allowed us to distinguish among the closely related organisms A. actinomycetemcomitans, H. aphro- philus, and H. paraphrophiius. A. actinomycetemcomitans was divided into two groups of strains. A. lignieresii fell outside or on the border of the A. actinobacillus class. A. ureae, H. injluenzae, H. parainjluenzae, P. haemolytica, and P. multocida fell outside the A. actinomycetemcomitans, H. aphrophilus, and H. paraphro- philus classes. Organisms belonging to the Actinobacillus-Haemophilus- The enzymic characterization data were treated statisti- Pasteurella group, which constitute the family Pasteurel- cally by using multivariate analyses. We have previously laceae, have assumed increasing clinical importance in med- used such methods as auxiliary techniques to define the icine and dentistry over the last few years (see references 30, following bacterial and yeast genera: Actinobacillus, Hae- 34, 38, 39).
    [Show full text]