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Laboratory Safety Manual

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Laboratory Safety Manual Laboratory Safety Manual Baylor University EHS Updated July 2017 CONTENTS Section 1: Laboratory Safety ......................................................................................................................... 1 Introduction ................................................................................................................................................ 1 The OSHA Laboratory Standard ............................................................................................................... 1 Policies ...................................................................................................................................................... 2 Roles and Responsibilities ........................................................................................................................ 3 Section 2: Chemical Hygiene Plans (CHP) ................................................................................................... 5 Section 3: Emergency Procedures ............................................................................................................... 6 Fire ............................................................................................................................................................. 6 Medical Emergencies ................................................................................................................................ 7 Chemical Exposures .................................................................................................................................. 7 Emergency Information Postings .............................................................................................................. 8 Reporting Accidents and Injuries ............................................................................................................... 9 Section 4: Chemical and Hazard Identification ............................................................................................. 9 Labels ...................................................................................................................................................... 10 Safety Data Sheets .................................................................................................................................. 10 Section 5: Health Hazards of Chemicals .................................................................................................... 10 Introduction .............................................................................................................................................. 11 Basic Toxicology ...................................................................................................................................... 11 RISK = TOXICITY x EXPOSURE ............................................................................................................ 11 Routes of Entry ........................................................................................................................................ 11 Toxic Effects of Chemical Exposure ........................................................................................................ 14 Chemical Exposure Determination .......................................................................................................... 15 Section 6A: Controlling Chemical Exposure ............................................................................................... 17 General Principals ................................................................................................................................... 17 Section 6B: Fume Hoods and Laboratory Exposures ................................................................................. 18 Fume Hoods and Laboratory Ventilation ................................................................................................. 18 How a Fume Hood Works ....................................................................................................................... 18 Using Chemical Fume Hoods .................................................................................................................. 19 Good Work Practices ............................................................................................................................... 19 Common Misuses and Limitations .......................................................................................................... 22 The Fume Hood as a Storage Device ................................................................................................. 23 Hood Performance Indicators .................................................................................................................. 24 Continuous Monitoring Devices ........................................................................................................... 24 Hood Survey Sticker ............................................................................................................................ 24 Other Laboratory Exhaust Systems ........................................................................................................ 25 Standard Operating Conditions ............................................................................................................... 27 Section 6C: Personal Protective Equipment ............................................................................................... 28 Personal Protective Equipment ............................................................................................................... 28 Eye Protection ......................................................................................................................................... 29 Protective Clothing and Footwear ........................................................................................................... 30 Gloves ...................................................................................................................................................... 30 Proper Glove Removal ............................................................................................................................ 34 Hearing Protection ................................................................................................................................... 35 Respiratory Protection ............................................................................................................................. 35 Section 7: Safe Work Practices and Procedures ........................................................................................ 37 7A: General Work Practices ........................................................................................................................ 37 Before You Begin .................................................................................................................................... 37 Chemical Storage .................................................................................................................................... 37 Personal Behavior ................................................................................................................................... 44 Transporting Chemicals ........................................................................................................................... 45 Working with Scaled-Up Reactions ......................................................................................................... 45 Unattended Experiments ......................................................................................................................... 46 Working Alone ......................................................................................................................................... 46 Section 7B: Flammable Materials ............................................................................................................... 48 Properties of Flammable and Combustible Liquids ................................................................................. 48 Storage of Flammable and Combustible Liquids..................................................................................... 48 Handling Precautions .............................................................................................................................. 49 Flammable Aerosols ................................................................................................................................ 50 Flammable and Combustible Solids ........................................................................................................ 50 Catalyst Ignition ....................................................................................................................................... 51 Section 7C: Peroxide Forming Compounds and Reactives ........................................................................ 52 Recommended Work Practices ............................................................................................................... 52 Examples of Peroxidizable Compounds .................................................................................................. 53 Detection of Peroxides ............................................................................................................................ 54 Removal of Peroxides ............................................................................................................................
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