Parasit, hung. 23. 1990

Studies on from aquatic in Hungary Dr. Zlatka M. DIMITROV A1, Dr. Éva MURAI2 and Dr. Todor GENOV3 Department of Zoology, Higher Institute of Zootechnics and Veterinary Medicine, Stara Zagora, Bulgaria' — Department of Zoology, Hungarian Natural History Museum, Budapest, Hungary2 — Institute of Parasitology, Bulgarian Academy of Science*. Sofia, Bulgaria3

"Studies on Acanthocephala from aquatic birds in Hungary" - Dimitrova, Z. M., Murai, É. and Genov, T. - Parasit, hung., 23: 39-64. 1990.

ABSTRACT. A total of 7 aquatic species of four orders were found as hosts of acanthocephalans: 5 species of the family and one of the family Centrorhynchidae were established. Morphological descriptions are presented, illustrating their variability. Polymorphic diploinflatus, P. magnus, P. cf.phippsi and Southwellina hispida are reported first for the Hungarian fauna.

KEY WORDS: Acanthocephala, Polymorphidae, Centrorhynchidae, morphology, , intraspecific variability, aquatic birds, Hungary.

When reviewing the endoparasites of birds in the regions of Hortobágy and Biharugra, EDELÉNYI (1964) described two acanthocephalan species from aquatic birds: Polymorphic striatus (Goeze, 1782) in anseriform and cico-niiform hosts, and anatis (Schrank, 1788) in anseriform and gruiform birds. MURAI et al. (1983, 1985) recorded in Hortobágy National Park and Kiskunság National Park the following acanthocephalan parasites from aquatic birds: Polymorphic minutie (Goeze, 1872), (Schrank, 1788), Centrorhynchus aluconis (Mueller, 1780) and C. lancea (Westrumb, 1821).

The aim of the present paper is to present new data on the species compo-sition, morphology and distribution of acanthocephalans from aquatic birds in Hungary. It is based on new specimens from anseriform, gruiform, ciconiiform and charadriiform hosts collected in different parts of the country and also on an already published material (MURAI et al. 1983) from Hortobágy National Park, used for further morphological investigations.

MATERIAL AND METHODS

Acanthocephalans were found in 7 bird species (Table 1).

The specimens were fixed in 70 % alcohol. They were cleared in glycerol (25-100 %) or lactic acid and studied on temporary slides. When the metasomes of a part of the specimens were not sufficiently cleared they were heated in lactic acid until achieving satisfactory results (KHOKHLOVA 1965). Table 1 Quantitative characteristics of acanthocephalan invasion of hosts

Hosts Acanthocephalans Prevalence Intensity

(in number) (in number)

I Ci coniiformes

1 Egretta alba Southwellina hispida 1/1 10 (2m,8f)

II Anseriformes

2 Anas platyrhynchos Filicollis anatis 4/16 2(f);2(lm,lf) ; 2(m);l(f) diploinflatus 1/16 5(f) 3 Aythya fuligula Polymorphus magnus 1/6 2(f) 4 Somateria mollissima Polymorphus cf. phippsi 1/1 2(lm,If) 5 Bucephala clangula Filicollis anatis 1/3 l(m)

III Gruiformes

6 Fulica atra Filicollis anatis 6/30 2(f);l(m); 2(lm,lf);4(f) ; 3(f);i(f) IV Charadriiformes

7 Vanellus vanellus Sphaerirostris lancea 2/22 3(f);3(f)

Remarks: m=male; f=female

The specimens were studied in an with "Amplival" microscope. The drawings were made with a drawing tube. The measurements in the text are given in millimeters and those in Tables 4, 6, 7 and 9 in /xm.

The species are arranged according to AMIN (1985). The species are deposited in the parasite collection of the Hungarian Natural History Museum. They all bear host collection numbers.

RESULTS AND DISCUSSION

Family CENTRORHYNCHIDAE Sphaerirostris lancea (Westrumb, 1821)

Specimens studied: No. 398, 3 females from small intestines of Vanellus vanellus, Hortobágy, April 1964; No. 661, 3 females from Vanellus vanellus, Kiskunság, Szabadszállás, April 1966. Description:

Female (Fig. 1, Table 2): Trunk fusiform, elongate, 16.35-18.60 long and with maximum width at anterior part - 1.50-1.89; the next part of trunk narrowed, 0.60-0.75 wide. Proboscis slightly constricted at level of insertion of proboscis receptacle: 0.563-0.606 long and with maximum width 0.331-0.365 (anterior part of proboscides are invaginated); width of the proboscis base 0.281-0.344; width of constricted place 0.281- 0.325. Armature consisting of 34-36 longitudinal rows of 11-13 hooks in each. Firs 5-6 hooks with well- developed roots, directed backward, the others with rudimentary roots. Sizes of hooks (blade x root) are presented in Table 2. The largest of them are 3rd and 4th, up to 0.0450 x 0.0550 - 0.0625. Neck short and almost invaginated. Proboscis receptacle sac-like, double-wall-ed, 0.80-0.99 long and maximum 0.24-0.25 wide. Trunk full of eggs; eggs elliptical, 0.0375-0.0575 long and 0.0175-0.0225 wide; without polar swellings of middle shell.

Fig. 1: Sphaerirostris lancea - N 661-2: a - female (anterior part); b - proboscis; c - longitudinal row of hooks; d - egg. Table 2 Metrie data of Sphaerirostris lancea from Vanellus vanellus

661-1 -3 (female 398 (female)

CHARACTERISTICS

n min -max x n min-max

Total length 3 16.94 -19.33 18 326 1 17.48 Trunk: length 3 16.35 -18.60 17 70 1 16.50 width 3 1.50 -1.89 1 73 1 1.56 width (posterior part) 3 0.60 -0.75 0 65 1 0.66 Proboscis : length 3 0.560 -0.606 0 586 1 0.731 max. width 3 0.331 -0.363 0 352 1 0.388 width of constric­ ted place 3 0.281 -0.325 0. 306 1 0.281 width ( part) 3 0.281 -0.344 0. 300 1 0.400 Number of longitudinal rows of hooks 3 34 -36 1 36 Number of hooks in each row 3 11 -12 1 13 Hook sizes: (blade x root) 2nd 0.035 -0.045 0.0375-0.0475 3rd 0 040 -0 .0450 xO.050-0. 0625 0 0375-0.0425x0.050 4th 0 035 -0 .0425 xO.035-0. 055 0.0425x0.0513 5th 0 030 -0 .0375 xO.035-0. 0375 0 0375x0.0375-0.040 6th 0 030 -0 .0375 xO.0275-0 .035 0.0325x0.0300 7th - 12th (13th) 0 025 -0 .0325 xO.0250-0 .030 0 0325x0.0250-0.030 Neck : length 1 0 120 1 0.250 width 1 0.440 Proboscis receptacle: - length 3 0.80 -0.99 1 0.85 width 3 0.24 -0.25 1 0.281 Lemnisci: length Eggs : length 0 .0375 -0.0575 0.0425-0.0550 width 0 .0175 -0.0225 0.0175-0.0275 compared to literature data of S. lancea and S. lanceoides

Sphaerirostris lancea Sphaerirostris lanceoides

SKRJABIN PETROCHENKO BELOPOL'SKAYA KHOKHLOVA BELOPOL1SKAYA 1913 1958 1983 1971 1983

Í2 - 20 9-22 1.70 1.5 0 - 2.50

0.600 0. 560 0.594 - 0.811 0.570 0.310-0.350 0.330

30 32 36 - 42 38 - 40

11 - 14 12 11 - 15 11 - 12

0 . 038x0.059 0.030-0.042x0.041-0.055 0.041 - 0.044 0.043 - 0.049

0.022 - 0.030 0.026 - 0.032 0.031 - 0.035

1.03-1.11 0.920 - 1.68 0.20-0.3 0

1 . 054 1.38 - 2.73

0.0440-0.0520 0.0670 - 0.0750 0.0520 0 . 0520-0.058 0.0180-0.0220 0.0250 0 . 0250-0.031 Remarks:

Three female specimens studied are close to S. lancea (Westrumb, 1821) and to S. lanceoides (Petrochenko, 1949) Golvan, 1956 both from passeriform and charadriiform birds but they have 34-36 longitudinal rows of hooks compared to Sphaerirostris lancea with 30-32 rows (PETROCHENKO 1958, BELOPOLSKAYA 1983) and S. lanceoides with 36-40 rows (KHOKHLOVA 1971, BELOPOLSKAYA 1983). Another difference of our specimens in the blade length - it is up to 0.450 and is closer to S. lancea - 0.044 - than to S. lanceoides - 0.049. The measurements of the specimens studied, compared with data of the literature, are shown in Table 2. For lack of material (missing males) we were not able to differentiate it quite definitely as S. lancea.

S. lancea is a common parasite of charadriiform and passeriform birds and an acanthocephalan widespread in Europe, China, Taiwan, Egypt and the U.S.A. (SCHMIDT 1969, KHOKHLOVA 1986). MURAI et al. (1983) reported 5. lancea from Vanellus vanellus and Philomachuspugnax in Hungary for the first time.

Family POLYMORPHIDAE

Filicollis anatis (Schrank, 1788)

Specimens studied: írom Anas platyrhynchos: No. 1564, 2 females, Hortobágy, July 1968; No. 2092, 1 male and 1 female, Hortobágy, November 1970; No. 8150, 2 males, Péteri-tó, Kiskunság National Park, March 1979; No. 11331, 1 female, Ti-hany, March 1984; from Bucephala clangula: No. 9353, 1 male, Kisbalaton, January 1981; from Fulica atra: No. 739, 2 females, Hortobágy, September 1965; No. 3240, 1 male and 1 female, Agárd, September 1971; No. 3256, 1 male, Agárd, Sep-tember 1971; No. 3267, 4 females, Agárd, September 1971; No. 3268, 3 fema-les, Agárd, September 1971; No. 7297, 1 female, Hortobágy, September 1976.

Description (based on the specimens horn A. platyrhynchos, see Fig. 2):

Male: Total length 3.489-6.370. Trunk fusiform, 3.020-5.76 long and with maximum width 0.99-1.10. Anterior part (with length 0.469-0.500) of trunk covered with spines; spines situated in longitudinal rows of 7-8 spines each; length of spines 0.0125-0.027. Proboscis oviform, wider in its posterior half; 0.344-0.356 long and with maximum width 0.250-0.306. Armature consisting of 18-20 longitudinal rows of 9-11 hooks each. First 7-8 hooks uniform, with well-developed blades with length of the first 0.030-0.0325 and of 2nd- 8th 0.0375-0.040; blade length of last hooks 0.030-0.0325; only anterior hook roots clearly seen, 0.0275- 0.0375 long; other roots rudimentary (for further hook measurements see Table 4). Neck conical, 0.620 long and 0.320 wide at base. Proboscis receptacle double-walled, attached at proboscis base; 0.92-1.20 long, with maximum width 0.14. Lemnisci elongate, band-shaped, 1.05-1.72 long. Testes elliptic, si-tuated in longitudinal row in the wider part of trunk and slightly over-lapping one another; testes measure 0.82- 0.94 x 0.35-0.45. Cement glands 6 in number, drop-shaped, situated in compact group, 0.96-1.07 long. Ducts of cement glands 0.70-0.95 long. Genital bursa 0.65-1.00 long.

Metric data on the males from A platyrhynchos, B. clangula and F. atra are presented in Table 3 (Fig. 3). Female: Total length 10.44-14.76. Trunk from fusiform to band-shaped, 6.45-11.34 long and with maximum width 1.92-3.24. Proboscis bulbous: 1.44-1.89 long and with width 1.86-2.13. Armature consisting of rosette of ru-dimentary hooks situated on the anterior part of bulbus. Neck elongate, cylindrical, 1.71- 2.85 long and 0.24-0.39 wide. Proboscis receptacle 2.70 long, with maximum width 0.10. Lemnisci band- shaped, 3.00-3.90 long and 0.21-0.24 wide. Eggs cannot be studied in specimens available.

For measurements of the females from A platyrhynchos and F. atra see Table 3.

Fig. 2: Filicollis anatis - N 8150-2: a - male; b - longitudinal row of hooks. Table 3 Metrie data of Filicollis anatis from different hosts

Ana s p 1 a t y r h y n c h o s

CHARACTERISTICS

male female

n min -max X n min-max X

Total length 3 3 .487 -6 . 370 5 .437 3 10 .44-14 .76 12 . 27 Trunk: length 3 3 . 020 -5. 760 4 . 757 4 6 .45-11 . 34 8 . 61 max. width 3 0 . 990 -1. 100 1 . 030 4 1 .92- 3 .24 2 . 69 Armed trunk zone: length 3 0 . 469 -0. 500 0 .483 - - Spines : Number of longitudinal rows - Number of spines in each row 3 7 -8 - - length 3 0 .0125 -0. 0275 0 . 0208 - - Proboscis (bulbus): length 3 0 .3438 -0. 3563 0 . 348 4 1 .44- 1 .89 1 . 65 width (ap. part) 3 0 . 0625-0 . 1250 Ó . 092 max. width 3 0 .250 -0. 3063 0 . 273 3 1 .86- 2 . 13 2 . 01 width (basal part) 3 0 . 175 -0. 2375 - - +Hooks: Number of longitudinal rows 3 18 -20 - Number of hooks in each row 3 9-10-11 Neck : - length 1 0. 620 - 5 1 .71- 2 . 85 2 .07 max. width 1 0 .32 0 5 0 .24- 0 . 39 0 .32 Proboscis receptacle : - length 3 0 .92 -1. 20 1 073 1 2.70 max. width 1 0. 14 - 1 0.10 Lemnisci: length 3 1 . 05 -1. 72 1 49 3 3 . 00- 3 .90 3 . 50 max. width - - 3 0 .21- 0 .24 0 .22 Genitals : testes (2) - length 2 0 82 -0 . 94 max. width 2 0 35 -0. 45 Cement glands (6) length of group: 2 0 96 -1. 07 1 06 Ducts of cement glands length 2 0 70 -0. 95 0 83 Genital bursa: length 2 0 65 -1. 00 0 83 Eggs : length egg balls width

+ The measurements of hooks are shown in Table 4 Table 3 continued

Bucephala Fulica atra

clangula

male male female

min-max min-max min-max

8.944 2.326-5.481 3.904 9.10-24.95

7. 800 1.920-4.500 3 . 210 10 4.64-20.25 1. 290 0.90 1.59- 5.25

0. 531

40 6-7 0.0275-0.30 0 . 0175

0. 456 0.406-0.481 0.444 1.50- 3.75 0. 125 0.069-0.094 0.081 1.98- 3.90 0.281 0.263-0.288 0.275 1.98- 3.90 0.219 0.219

18 19-20 1 0 10-11

0. 688 0. 50 1.50- 2.10 0.425 0.30- 0.39

1.38 1.35 0 . 15 0. 14

1.72 0. 08

0.98-1.06 0.850 0.48-0.53 0.400

1.26

1. 11

1.11

0.0594-0.0688 0.0250-0.0314 Table 4 Metrie data of the hooks of Filicollis anatis from different hosts . Measurements in pm.

Anas platyrhynchos Bucephala clangula Fulica atra 1 Blade

2 Root n min-max x n min-max X n min-max X

1st row 1 length 3 30 0-32 . 5 31.7 2 40.0-42. 5 41. 3 4 37. 5-40. 0 39. 4 thickness 4 7 5-10.0 8.8 2 11.3-11. 3 11.3 4 7. 5-10. 0 9 . 4 2 length - - - 1 40.0 - 7 35. 0-36. 3 35 . 7 thickness ------7 7. 5- 7. 5 7. 5 Und row 1 length 5 37 5-37.5 37. 5 2 42.5-42 5 42.5 4 43 8-45 0 44 . 7 thickness 5 10 0-12.5 9.0 2 12.5-12 5 12.5 4 12 5-12 5 12 5 2 length 1 35 0 - 2 45.0-47 5 46.3 3 32 5-40 0 36. 7 thickness - - - 2 10.0-10 0 10.0 3 10 0-10 0 10 0

Illrd row 1 length 5 37 5-40.0 38.3 2 42.5-42 5 42 . 5 4 45 0-45 0 45 0 thickness 5 1 7 5-12.5 12 . 5 2 15.0-16 3 15.6 '1 i j 8-15 0 14 4 2 length 3 35 0-37.5 35.8 2 45.0-45 Ü 45.0 3 3b 0-40 0 38 3 thickness - - - 2 10.0-10 0 10.0 3 10 0-10 0 10 0

IVth row 1 length 5 10 0-40.0 40.0 2 42.5-43 7 43.1 4 42 5-47 5 45 0 thickness 5 12 5-12.5 12.5 2 15.0-17 5 16.3 4 12 5-15 0 14 4 2 length 5 35 0-40.0 37.5 2 45.0-47 5 46.3 3 3b 0-40 0 3 7 9 thickness 3 10 0-12.5 11.0 2 10.0-10 0 10. 0 3 10 0-10 0 10 0

Vth row 1 length 6 38 8-40.0 39.8 2 42.5-42 5 42.5 4 4 0 0-47 5 43 1 thickness 6 10 0-12.5 12.1 2 13.8-15 0 14.4 4 12 5-13 8 12 8 2 length 4 30 0-40.0 35.0 2 37.5-41 3 39.4 3 32 5-37 5 3 5 0 thickness 2 10 0-10.0 10.0 2 10.0-10 0 10.0 3 10 0-10 0 10 0

Vlth row 1 length 6 37 5-40.0 39.0 2 42.5-42 5 42.5 4 4 0 0-45 0 42 b thickness 6 7 5-12.5 10.0 2 12.5-12 5 12. 5 4 10 0-12 5 11 0 2 length 1 2 2 5 - 1 20.0 - 2 25 0-32 5 28 6 thickness ------2 10 0-10 0 10 0 Vllth row 1 length 6 37 5-38.8 37.9 2 42.5-45 0 43.8 4 37 5-45 0 40 6 thickness 6 7 5-10.0 8.9 2 12.5-12 5 12.5 4 10 0-12 5 11 0 2 length - - 2 18.8-25 0 21.9 1 25 0 thickness -

Vlllth row 1 length 6 35 .0-37.5 36.3 2 41.1-42 .5 41.8 4 35 0-40 0 37 5 thickness 6 7 .5-10.0 8.6 2 10.0-11 .3 11.0 4 7 5-10 0 9 4 2 length - - - 2 15.0-17 .5 16.3 1 17 5 thickness

IXth row 1 length 5 32 .5-37.5 35.5 2 37.5-37 . 5 37.5 4 30 . 0-35 0 3 2 5 thickness 5 5 .0-10.0 7.9 2 8.8- 8 8 8.8 3 7 . 5-10 0 8 3 2 length - - 2 12.5-13 . 8 13.2 - thickness -

Xth row 1 length 5 30 .0-32.5 31.0 2 37.5-37 . 5 37.5 2 30 .0-32 5 31 3 thickness 5 5 .0- 5.0 5.0 2 8.8- 8 .8 8.8 2 7 .5- 7 .5 7 .5 2 length - - - 1 10.0 - - thickness

Xlth row 1 length - - _ _ _ _ 2 27 . 5-27 .5 27 .5 thickness - - - - - 2 7 .5- 7 .5 7 .5 2 length - thickness Fig. 3: Filicollis anatis - N 9353: a - male; b - proboscis; c - longitudinal rows of hooks; N 3256 (male): d - proboscis; e - longitudinal row of hooks; N 3268-1: f - egg. Remarks:

F. anatis is considered to be one of the most frequent and wide-spread acanthocephalans of waterfowl in the Palaearctic (KHOKHLOVA 1986). It was reported in Hungary by EDELÉNYI (1964) and MURAI et al. (1983, 1985) from several aquatic bird species.

The comparison of morphological data of specimens studied from different hosts (Table 4) shows a considerable variability of the male proboscis and hook measurements.

There are only two descriptions of this species in the available litera-ture: that of LUEHE (1911) which has been completed by PETROCHENKO (1958) and that of ATRASHKEVICH (1982) which is the only one based on specimens from concrete host and region (Anas acuta in Chukotka). The former des­ cription is also presented by KHOKHLOVA (1986). Only the hook dimensions of our specimens fromA platyrhynchos correspond to the measurements given by ATRASHKEVICH (1982) for F. anatis (blade length of the first 7 hooks is up to 0.041). The specimens from B. clangula and F. atra have larger hooks (see Table 4) and they are more similar in this feature to F. trophimenkoi Atrashkevich, 1982 (blade length up to 0.048).

Another substantial difference among the male specimens studied concerns the shape of the proboscis. The worms from A platyrhynchos have oviform proboscides while those from B. clangula and F. atra have anteriorly rounded proboscides with a form which is rather oval than oviform. The proboscis dimensions of the specimens from B. clangula and F. atra are considerably larger than both those from A. platyrhynchos in the present study and those in the published descriptions (according to PETROCHENKO (1958) proboscis is 0.36-0.40 long and according to ATRASHKEVICH (1982) it is 0.329-0.379). In specimens of F. anatis from F. atra from Bulgarian Black Sea Coast (with proboscis length 0.347-0.410) (DIMITROVA and GENOV, in press) was observed similar but not so clear exceeding the known limits of variability in proboscis dimensions.

The taxonomic value of differences found is quite unclear. Although F. anatis is considered a common parasite of waterfowl, its variability is insufficiently known. It is possible that the differences result from in­ dividual or host-dependent variability. It may well be that two or more similar species are reported as F. anatis. The first steps in the direction of solving these problems are the investigations of RUDMINAYTIS and KONTRIMAVICHUS (1981) and ATRASHKEVICH (1982).

Polymorphus (P.) diploinflatus (Lundstrom, 1942)

Specimens studied: No. 2118, 5 females from small intestines of Anas platyrhynchos, Hortobágy, January 1971.

Description (Tables 5, 6, Fig. 4):

Female: Total length 4.78-6.57. Trunk fusiform with characteristic constriction dividing anterior and middle part; latter part widest; 4.08-5.13 long and with maximum width 1.04-1.59. Posterior part greatly narrowed, 0.61-0.72 wide. Anterior part (with length 0.88-1.02) of trunk covered with spines, spines situated in 50-60 longitudinal rows of 20-27 each; length of spines 0.020-0.025. Proboscis elongate, oval, 0.563-0.613 long and with maximum width 0.263-0.306 at the level of 3rd-4th spines. Armature consisting of 14 longitudinal rows of 8-9 hooks each. First 4-5 hooks considerably larger than the others, with well-developed roots. Third and fourth hooks largest, with blade up to 0.0725 long and root 0.070-0.075 long. Measurements of hooks are presented in Table 6. Neck conical, 0.800-0.875 long. Proboscis receptacle double-walled, attached at proboscis base; 1.20-1.44 long, 0.15-0.27 wide. Lemnisci digitiform, longer than proboscis receptacle, 1.20-1.75 long and 0.45 wide. Trunk filled with eggs 0.053- 0.070 long and 0.013-0.019 wide. In some specimens visible egg balls measuring 0.144.

Fig.4: Polymorphus diploinflatus - N 2118-1, 4: a - female; b - proboscis; c - d - longitudinal rows of hooks. Table 5 Metrie data of Polymorphus diploinflatus compared to data of the literature

Polymorphus According to literature

CHARACTERISTICS diploinflatus Lundström Khokhlova Shirinov No 2118 1942 1966b 1962

Total length 4. 78-6.57 5.7-6.0 3.15-4.26 4.483 Trunk: length 4.08-5.13 5.7-6.0 3.15-4.26 4.483 max. width 1.04-1.59 0.7-1.1 0.856-1.396 1.1011 Armed trunk zone: length /0.8 8-1.05/;/0.96-1. 20/ 0.766-1.226 Spines : Number of longitudinal rows 50-60 40-50 Number of spines in each row: 20-27 length 0.020-0.025 0 .021-0.024 0.028-0.034 Proboscis : length 0.563-0.613 0. 600 0.460-0.550 0. 5505 max. width 0.263-0.306 0.213-0.306 0.2674 Number of longitudinal rows of hooks 14 14- 14(13-16) 14 Number of hooks in each row 8-9 8-9 7-8(6-9) 8-9 Hook sizes: (blade x root) 1st 0 0562 5-0.0675x0.050-0 .060 0 .057-0.069 0.065x0.055 0 0583-0.0648 2nd - 4th 0 0650 -0.0750x0.0575-0 .075 0.074-0.055x0.040-0.062 5th 0 0525 -0.0575x0.0275-0 .0375 0.045 0.049x0.034 6th - 8th (9th) 0. 055-0.0425x0.0250 0.031x0.031 0 0421-0.0453 Neck: length 0.800-0.875 1. 10 0.0475-0.0766 Proboscis receptacle: length 1.20-1.44 0.613-1.226 1.1483 width 0.15-0.27 - 0.1415 Lemnisci: - length 1.20-1.75 - 0.766-1.38 - width 0.40 Eggs : - - - length 0.0525-0.070 0 .090-0.100 0.108 width 0.0125-0.0188 0.018 0.016-0.018 - Table 6 Metrie data of the hooks of Polymorphus diploinflatus

from Anas platyrhynchos - No 2118

Measurements in /xm

N Blade Root

length thickness length thickness

1 56 3-67. 5 12 5-15 0 50 0-60 0 10.0-11.3

2 65 0-70, 0 15 0-18 8 57 5-62 5 11. 3-13.8

3 67 5-72 5 17 5-20 0 62 5-70 0 12.5-15.0

4 65 0-75. 0 20 0-25 0 62 5-75 0 12.5-17.5

5 52 5-57 5 12 5-17 5 37 5-27 5 10.0-17.5 6 50 0-55 0 12 5-16 3 25 0-27 5 - 7 47 5-55 0 12 5-15 0 12 5-15 0 - 8 47 5-55 0 12 5-16 3 25 0 - 9 42 5-50 0 12 5-15 0 - -

Remarks:

First LUNDSTROM (1942), cited by PETROCHENKO (1958), described these spe-cies from Anas platyrhynchos in Sweden. There are some descriptions in the literature illustrating its variability. Metric data of specimens studied compared to the literature (according to LUNDSTROM 1942, SHIRINOV 1962a and KHOKHLOVA 1966b) are presented in Table 5. The specimens studied are morphologically similar to descriptions in the literature. The differences concern length measurements of some of the proboscis which in our speci-mens are up to 0.613 (0.60 in LUNDSTROM 1942); the last hooks which are larger (0.0425-0.055 compared to SHIRINOV 1962a - 0.0421-0.0453); the eggs which are shorter (up to 0.070) compared to LUNDSTROM 1942 (0.09-0.10) and KHOKHLOVA 1966b (up to 0.108).

Polymorphus diploinflatus was reported from Azerbaijan SSR (SHIRINOV 1962a,b; VAIDOVA 1963), from Chukotka (KHOKHLOVA 1966b) and from Bulgaria (TSACHEVA 1967). P. diploinflatus is first reported for the Hungarian fauna. Polymorphus magnus (Skrjabin, 1913)

Specimens studied: No. 10607, 2 females from Ayihya fuligula, Biharugra, November 1985.

Description (Table 7, Fig. 5): Female: Total length 10.29-12.54. Trunk fusiform, with constriction after its anterior widest part; 10.0-11.4 long and with maximum width 1.65-1.95. Anterior part (with length 1.35-1.40) of trunk covered with spines; length of spines 0.0175-0.0225. Proboscides of both specimens with not fully everted anterior parts; 0.294-0.438 long, with maximum width 0.244-0.263 and width at base 0.169-0.175. Armature consisting of 16 longitudinal rows of hooks; only posterior 5-6 hooks in each row seen, anterior hooks not studied. Largest hooks seen with blade 0.0575-0.0613 long and root 0.075-0.0775 long. Neck conical, 0.70 long and 0.33 wide. Proboscis receptacle double-walled, attached at proboscis base; 1.38-1.50 long, with maximum width 0.17. Lemnisci 2.70 long. Trunk full of eggs. Eggs elongate ellip-tic; external shell with many fine fibrillar structures; middle shell with polar swellings; eggs measure 0.088-0.144 x 0.016-0.025.

Remarks:

The specimens studied are morphologically similar to the description of PETROCHENKO (1958) which includes SKRJABIN's original description and is completed with additional data. The main differences concern the measurements of the roots of the largest hooks which in our specimens are up to 0.075-0.0775 (0.0712 in PETROCHENKO's description) and slightly larger eggs with maximum length 0.144 (0.139 according to the cited description).

Table 7 Metric data of the hooks of Polymorphus magnus

from Aythya fuligula - No 10607

Measurements in p.m

N Blade Root

length thickness length thickness

1 57 5-57 5 15 0-16 3 60.0-62 5 15.0-15.0

2 60 0-61 3 17 5-20 0 77.5-77 5 17 . 5-17 . 5

3 57 5-60 0 20 0-22 5 75.0-75 0 20 . 0-22.5

4 47 5-50 0 12 5-15 0 - - 5 40 0-45 0 12 5-15 0 - - 6 32 5

The anterior hooks (1-2) are inserted P. magnus is one of the most widespread acanthocephalans of waterfowl in East Europe and Central Asia (KHOKHLOVA 1986). This is its first record for Hungary.

Fig. 5: Polymorphus magnus - N 10607-1-2: a - female (anterior part); b - proboscis; c - longitudinal row hooks; d - egg. Polymorphus cf. phippsi (Kostylew, 1922)

Specimens studied: No. 12028, 1 male and 1 female from Somateria mollissima, County Győr-Sopron, Mexikópuszta, September 1988. The material is not well preserved.

Description (Fig. 6):

Male: Trunk fusiform, wider in middle part, 9.96 long and with maximum width 1.86. Anterior part (with length 0.59) of trunk covered with spines; spines situated in about 36-40 longitudinal rows; length of spines 0.020-0.025. Proboscis with not fully everted anterior part; 0.269 long, with maximum width 0.244 and width at base 0.238. Armature consisting of 14 longitudinal rows of hooks; only 4 not well-preserved hooks

Fig. 6: Polymorphus cf. phippsi - N 12028-1-2: a - a male; b - proboscis of male; c - spines of trunk; d - proboscis of female; e - longitudinal row of hooks. seen in each row (others on not everted part). Hooks seen with measurements as follows (blade x root; from anterior to posterior hooks): 0.0625-0.0638 x 0.0675; 0.0575-0.0638 x 0.0625-0.0650; 0.0550-0.0625 x 0.0575; 0.0550 x 0.030. Neck elongate, conical, 0.450 long and with maximum width 0.375. Proboscis receptacle and lemnisci not studied. Testes elongate, oval; situated one behind other; measure 1.0-1.14 x 0.60-0.75. Cement glands 4 in number, tubular; two of them slightly larger (3.75 x 0.16) than other ones (3.00 x 0.16). Ducts of cement glands and genital bursa deformed.

Female: Trunk elongate, without constriction, 7.05 long and with maximum width 1.95 in its posterior part. Anterior part (with length 1.80) of trunk covered with spines; spines situated in about 36-40 longitudinal rows of 19-20 hooks each. Proboscis almost oval; anterior part not evert-ed; 0.325 long, with maximum width 0.238 and width at base 0.175. Ar-mature consisting of 14 longitudinal rows; only 5 not well- preserved hooks seen in each row (others on not everted part). Hooks seen with measure-ments as follows (blade x root; from anterior to posterior hooks): 0.0600 x 0.0675; 0.0625-0.065 x 0.0625; 0.0575; 0.0550- 0.0575; 0.575. Neck elon-gate, almost cylindrical, 0.070 (part of it invaginated in trunk) and 0.188 wide. Proboscis receptacle double-walled, attached at proboscis base; 1.35 long, with maximum width 0.210 at distal end and width 0.163 at proboscis base. Lemnisci not studied. Trunk full of eggs. Eggs elliptical, elongate, 0.093-0.100 long and 0.018-0.020 wide; with polar swellings of middle shell.

Remarks:

The present material fully corresponds to P. phippsi, a species originally described from the same host and after that reported from different aquatic birds. There are, however, several differences: the dimensions of the third-fourth hook in our material is smaller (up to 0.065) than these in the descriptions of KOSTYLEW (1922, cited after PETROCHENKO 1958) and KHOKHLOVA (1966) - 0.068-0.071; the length of the cement glands of the Hungarian specimens is greater (3.00-3.75) in comparison with the cited descriptions (2.1-2.43). Unfortunately, the bad condition of the specimens does not permit more adequate comparisons.

Southwellina htspida (Van Cleave, 1925) Witenberg, 1932

Specimens studied: No. 12334, 2 males (1,7) and 8 females (2, 3, 4, 5, 6, 8, 9, 10) from Egretta alba, Kiskunság, Solt, April 1990.

Description (Tables 8, 9, Fig. 7):

Male: Total length 8.43-10.00. Trunk fusiform, with characteristic con-striction in its anterior part; 5.79- 6.06 long and with maximum width 1.38-1.41 about constricted place; posterior trunk part narrowed, 0.060-0.084. Anterior part of trunk with two zones of spines; first zone (with length 0.025-0.030) separated from neck by narrow unarmed collar-like structure; length of spines 0.0225-0.0275. In second zone (with length 0.50-0.78) spines situated in 72-92 longitudinal rows of 8-10 hooks each; spines triangular, 0.0175- 0.0375 long. Unarmed zone with length 0.137-0.230 between two armed zones. Neck conical, 0.350 long and 0.480 wide. Proboscis cylindrical, widest in posterior part; 0.90-0.91 long, with maximum width 0.263- 0.338 in posterior part and width 0.188-0.225 in ante-rior part. Armature consisting of 19-20 longitudinal rows of 16-17 hooks each. First 8-9 hooks with well-developed blades: 1st 0.050-0.0525 long, 2nd-9th 0.060-0.070 (one of specimens with considerably longer hooks); root gradually longer, directed backward, 0.0375-0.0650 long; usually 8th-9th hooks with most massive root. Following lOth-llth hooks with shorter blades: 0.0475-0.0525 and 0.055-0.0575; 12th-17th hooks longer - 0.0575-0.0625, with shorter and narrower roots (0.0275-0.0375, only once 0.0425) directed forward (for more detailed data on hook Table 8 Metric data of Southwellina hispida from Egretta alba (L.) - N 12334

1,7 2 ,3,4,5,6,8,9, 10 (males) (females) CHARACTERISTICS

n min-max x n min-max x

Total length: 2 6.70-7.50 7 10 4 8.43-10.06 9.15 Trunk: length 2 5.79-6.06 5 93 8 6.33-12.50 8.47 max. width 2 1.38-1.14 1 35 7 1.29- 1.98 width of posterior part 2 0.60-0.84 0 72 8 0.75- 1.11 0.8- 8 1st armed trunk zone: length : 0.25-0.30 3 0.23- 0.30 0.26 length of spines 0.0225-0.0275 1 0.016-0.0175 2nd armed trunk zone: length 2 0.50-0.78 0 67 8 * 0.65-0.96 0.835 Number of longitudinal rows of spine 2 70-84 77 6 72-92 85 Number of spines in each row 2 8-10 8 10-12(13) Length of spines 2 0.0175-0.0375 8 0.010-0.0375 Unarmed zone of spine: length 2 0.13-0.23 5 0.14- 0.27 Proboscis : length 2 0.90-0.91 0 905 3 0.86- 0.95 0.91 width of anterior part 2 0.188-0.225 0 206 6 0.219-0.260 0.237 max. width 2 0.263-0.338 0 300 6 0.270-0.375 0. 350 width of basal part 2 0.194-0.225 0 .210 5 0.206-0.256 0.232 +Hooks: Number of longitudinal rows of hooks 2 19-20 8 20-21 Number of hooks in each row 2 16-17(15) 3 16 Neck: length 1 0.350 2 0.45-0.45 0.45 width 1 0.480 3 0.51-0.60 0.57 Proboscis receptacle: length 2 1.32-1.58 1 .45 1 1.20 - width 2 0.373-0.420 0 .397 - - Lemnisci : length 2 1.56-1.58 1 .57 3 1.55-1.80 1.67 width 2 0.21-0.24 0 .22 - - Genitals: testes (2) length x width 2 0. 60x0.50 - Cement glands (4) length 1 1.80 Ducts of cement glands (2) length 1 0.90 Genital bursa: length 1 1.05 Eggs : - length 8(10) 0.106-0.137 0.121 width 8(10) 0.030-0.037 0.0327

+ The measurements of hooks are given in Table 9 measurements see Table 9). Proboscis receptacle sac-like, double-walled, attached at proboscis base; 1.32- 1.58 long, with maximum width 0.373-0.42. Lemnisci leaf-like, extending up to anterior testis; 1.56-1.58 long and 0.21-0.24 wide. Testes oval, situated diagonally in widest part of trunk; testes measure 0.60 x 0.45- 0.60. Cement glands 4 in number, tubular; 1.80 long. Ducts of cement glands 0.90 long. Genital bursa 1.05 long. Genital pore terminal.

Fig. 7: Southwellina hispida - N 12334-1-2: a - male; b - proboscis of male; c - longitudinal row of hooks; d - egg- Table 9 Metric data of the hooks of Southwellina hispida

from Egretta alba (No 12334). Measurements in p,m.

males females Serial No (1, 7) (2, 3, 5) 1 Blade of hook 2 Root rows n min-max X n min-max K

1st row l length 4 50. 0-52 . 5 51 . 3 1 52 .5 thickness 3 10. 0-10 . 0 10 . Ü 1 16 . 3 2 length 2 32. 5-37 . 5 35 . 0 1 42 .5 thickness - -

Und row 1 length 4 57 . 5-62 . 5 60 . 0 7 55 .0-62. 5 60 . 7 thickness 4 12 . 5-15. 0 14 . 4 7 15 .0-17. 5 16 . 4 2 length 4 37 . 5-45. 0 41 . 9 4 37 .5-45. 0 43 . 1 thickness 2 12 . 5-12 . 5 12 . 5 3 15 .0-15. 0 15 . 0

Illrd row 1 length 4 62 . 5-67 . 5 65 . 3 8 60 .0-62. 5 61 . 9 thickness 4 15 . 0-17 . 5 16 . 3 8 15 .0-17. 5 16 . 6 2 length 4 45. 0-50 . 0 46 . 9 6 42 .5-52. 5 48 . 6 thickness 3 12. 5-13 . 8 12 .9 2 15 .0-15. 0 15 . 0

IVth row 1 length 4 65. 0-70. 0 66 . 9 8 60 .0-65. 0 62 . 5 thickness 4 15. 0-20. 0 17 . 8 8 15 .0-20. 0 18 . 0 2 length 4 45. 0-50. 0 48 . 6 5 47 .5-55. 0 50 . 5 thickness 3 12 . 5-15. 0 14 . 0 2 17 5-17 . 5 17 . 5

Vth row 1 length 4 62 . 5-70. 0 65 . 9 7 60 .0-65. 0 62 . 1 thickness 4 15. 0-20. 0 18 . 2 8 15 .0-20. 0 17 . 8 2 length 4 45. 0-52 . 5 50 . 0 3 50 . 0-57. 5 53 . 3 thickness 3 12 . 5-17 . 5 15 . 0 2 17 . 5-17. 5 17 .5

Vlth row 1 length 4 62. 5-65. 0 63 . 1 8 60 .0-62. 5 62 .2 thickness 4 15. 0-20 . 0 16 . 9 7 17 .5-20. 0 18 .8 2 length 4 45. 0-52 . 5 49 . 4 5 50 .0-57. 5 53 . 5 thickness 3 12 . 5-17 . 5 15 . 0 2 17 .5-17. 5 17 . 5

vi Ith row 1 length 4 57 . 5-65 . 0 61 . 9 7 60 .0-62 . 5 61 . 1 thickness 4 17 . 5-21. 3 19 . 1 6 17 .5-22. 5 20 . 0 2 length 4 50 . 0-60. 0 54 .4 7 50 .0-62. 5 55 . 7 thickness 4 17. 5-17. 5 17 . 5 4 20 .0-20. 0 20 . 0

viiith row 1 length 4 57 . 5-65 . 0 61 . 3 8 57 .5-65. 0 62 . 2 thickness 3 18 . 8-23 . 8 20 . 8 7 20 .0-22. 5 22 . 1 2 length 4 52 . 5-65. 0 58 . 8 7 60 .0-67. 5 63 . 9 thickness 4 17 . 5-20. 0 18 . 8 5 20 .0-22. 5 20 . 5 Table 9 continued

IXth row 1 length 3 55 . 0-62 . 5 59 . 1 7 50 . 0- 62 .5 55 . 5 thickness 3 18 . 8-20 .0 19 . 0 7 17 . 5-22 . 5 21 . 2 2 length 3 47 . 5-52 .5 50 . 0 7 45 . 0- 67 . 5 58 . 2 thickness 3 17 . 5- 1 8 . 8 17 . 9 3 17 . 5-20 . 0 19 . 2

Xth row 1 length 3 47 . 5- 50 . 0 48 . 3 7 47 . 5- 60 . 0 51 . 4 thickness 3 13 . 8- 15 . 0 14 . 2 6 15 . 0- 15 . 0 15 . 0 2 length 3 32 . 5-37 . 5 35 . 0 3 37 . 5-45 . 0 42 . 5 thickness 1 12 . 5 - - - - - Xlth row 1 length 3 52 . 5-57 . 5 55 . 0 7 55 . o- 60 . 0 58 . 9 thickness 3 12 . 5- 15 . 0 13 .8 5 15 . 0- 17 . 5 15 . 5 2 length 1 27 . 5 ------thickness ------Xllth row 1 length 3 60 . 0- 62 . 5 60 . 8 5 57 . 5-62 . 5 60 . 0 thickness 2 13 .8- 15 . 0 14 . 4 4 15 . 0- 17 . 5 16 . 0 2 length 1 37 . 5 - - - - - thickness — ------XHIth row 1 length 2 62 . 5-62 .5 62 . 5 4 62 . 5- 62 . 5 62 . 5 thickness 2 13 . 8- 13 .8 13 . 8 3 15 . 0- 15 . 0 15 . 0 2 length 1 37 . 5 ------thickness ------XIVth row 1 length 2 60 . 0- 62 . 5 61 . 3 2 62 . 5-62 . 5 62 . 5 thickness 2 12 . 5- 12 . 5 12 .5 2 15 . 0- 15 . 0 15 . 0 2 length 1 37 . 5 ------thickness ------XVth row 1 length 2 62 . 5- 65 . 0 63 . 8 1 62 . 5 — _ thickness 1 12 . 5 - - 1 15 . 0 - - 2 length 1 37 . 5 ------thickness ------XVI th row 1 length 2 57 . 5-65 . 0 60 . 0 1 65 . 0 _ _ thickness 1 12 . 5 - 1 15 . 0 - - 2 length 1 42 . 5 ------thickness ------XVIIth row 1 length 1 57 . 5 - - - _ _ _ thickness - - - 2 length -1 42 . 5 ------thickness - - - Female: Total length 8.43-13.00. Trunk fusiform, with characteristic con-striction in anterior trunk part; 6.33-12.50 long and with maximum width 1.29-1.98; posterior trunk part narrower, 0.75-1.11 wide. Anterior part of trunk covered with spines situated in two zones. Most specimens with ante-rior spinous zone invaginated in trunk. Anterior zone 0.231-0.300 long. Second spinous zone 0.790-0.835 long; spines situated in 72-92 longitu-dinal rows of 10-12 (13) hooks each; spines triangular, 0.010-0.0375 long. Neck conical, 0.45 long and 0.51-0.60 wide. Proboscis cylindrical widens in posterior third; 0.86-0.95 long, with maximum width 0.270-0.375 and width in anterior part 0.237. Armature consisting of 20-21 longitudinal rows of 16 hooks each. Pattern of hook position similar to that of males; for measurements see Table 9. Proboscis receptacle sac-like, double-wall-ed, attached at proboscis base; 1.20 long. Lemnisci elongate, 1.55-1.80. Trunk filled with eggs. Eggs elliptical, 0.106-0.137 long and 0.030-0.037 wide; with polar swellings of middle shell.

Remarks:

SCHMIDT (1973) resurrected the Southwellina (Witenberg, 1932) and recognized three valid species in it. Only S. hispida is wide-spread in the Palaearctic -eating birds and has been described several times under different names: Arythmorhynchus fuscus Harada, 1929, A. duo-cinctus Chandler, 1935, Polymorphus ardeae Belopol'skaya, 1958 and Hemiechinosoma ponticum Petrochenko et Smogorzhevskaya, 1962. These synonyms were accepted by KHOKHLOVA (1986). The present speci­ mens have larger eggs than these mentioned in all the previous descriptions: up to 0.138 in our material and up to 0.119 in the material from Crimea (SCHMIDT 1973).

S. hispida was recorded in Japan, Taiwan, Galapagos Islands, North America, Primor'e region and Ukraine (SCHMIDT 1973; KHOKHLOVA 1986). This is its first record for Hungary.

CONCLUSION

A total of 7 aquatic bird species of four orders were found as hosts of acanthocephalans of the order (Table 1). Polymorphus diploinflatus, P. magnus, P. cf. phippsi and Southwellina hispida are first recorded for the Hungarian fauna. AH the species are redescribed and figured on the basis of Hungarian specimens.

DIMITROV A, Z. M., MURAI É. és GENOV, T.: Vizsgálatok magyarországi vízimadarak Acanthocephala élősködőin

Hét magyarországi vizimadárból hat buzogányfejü-féreg fajt közölnek a szerzők (1.táblázat). Két faj már ismert volt faunaterületünkről: Filicollis anatis (Schrank, 1788) gazdái a tőkésréce, a kerceréce és a szárcsa; Sphaerirostris lancea (Westrumb, 1821) gazdája a bíbic. Négy faj új a magyarországi faunában: Southwellina hispida (Van Cleave, 1925) Witenberg, 1932 nagykócsagból; Polymorphus diploinflatus Lundström, 1942 tőkésrécéből; Polymorphus magnus Skrjabin, 1913 kontyos récéből; Polymorphus cf. phippsi Kostylew, 1922 pehelyrécéből került a Természettudományi Múzeum gyűjteményébe. REFERENCES

AMIN, O. M. (1985): Classification. In: CROMPTON, D.W.T. and NICKOL, B.B. (eds): Biology of the Acanthocephala. - Cambridge University Press, p. 27-72.

ATRASHKEVICH, G. I. (1982): Filicollis trophimenkoi sp. n. (Acanthocephala: Polymorphidae) from Anatidae in North-Western Chukotka (in Russian). - Parazitologiya, 16: 102-106.

BELOPOLSKAYA, M. N. (1983): Acanthocephalans of charadriiform birds from European part of USSR (in Russian). - Uchen. zap. Leningr. gos. Univ., Ser. Biol., 3: 17-25.

DIMITROVA, Z. M. and GENOV, T. P. (1991): New data of species and spreading of acanthocephalans of aquatic birds in Bulgaria. - Helminthologya (in press).

EDELÉNYI, B. (1964): A hazai madarak belsőélősködő férgei. III. (Endoparasitäre Würmer der einheimischen Vögel. III.) - A Debreceni Agrártud. Föisk. Tud. Közi., 10: 173-188.

KHOKHLOVA, I. G. (1965): About methods of study of acanthocephalan morphology. - Proc. Sei. Conf. Soc. helminthologists in USSR, Moscow, h 234-236.

KHOKHLOVA, I. G. (1966): Acanthocephalans of birds of Chukotka (in Russian). - Trudy GELAN, 17: 245-259.

KHOKHLOVA, I. G. (1971): Acanthocephalans of birds Yakut. ASSR (in Russian). - Trudy GELAN, 22: 215-223.

KHOKHLOVA, I. G. (1985): Phylum Acanthocephales (Rudolphi, 1808). In: RHYZHIKOV, K. M. and RYSAVY, B. (eds): Helminth of fish-eating birds of the Palaearctic Region II. Cestoda and Acanthocephales. - Academia, Publ. House, Moscow/Prague, pp. 412.

KHOKHLOVA, I. G. (1986): Acanthocephalans of terrestrial vertebrates of USSR fauna. - Izd. Nauka, Moscow, pp. 276.

MURAI, É.. SULGOSTOWSKA, T., MATSKÁSI, I., MÉSZÁROS, F. and MOLNÁR, K. (1983): Parasitic helminths of vertebrates (, amphibians, reptiles and birds) in the Hortobágy National Park. In: MAHUNKA, S. (ed.): The fauna of Hortobágy National Park, p. 15-30. - Akadémiai Kiadó, Budapest.

MURAI, É., SULGOSTOWSKA, T., MATSKÁSI, I. and MÉSZÁROS, F. (1986): Parasitic helminths of vertebrates (fishes, amphibians, reptiles, birds and mammals) in the Kiskunság National Park. In: MAHUNKA, S. (Ed.): The fauna of the Kiskunság National Park, h 61-78. - Akadémiai Kiadó, Budapest.

PETROCHENKO, V. I. (1958): Acanthocephala of domestic and wild . II. (in Russian). - Izd. Akad. Nauk USSR, Moscow, pp. 456. RUDMINAITIS, E. A. and KONTRIMAVICHUS, V. L. (1981): An electrophoretic study of differentiation of acanthocephalans of the genus Filicollis Luhe, 1911 (in Russian). - Doki. Akad. Nauk USSR, Ser. zool., 258: 511-512.

SCHMIDT, G. D. and KUNTZ, R. E. (1969): Centrorhynchus spilornae sp. n. (Acanthocephala) and other Centrorhynchidae from the Far East, - J. Parasit., 55: 329-344.

SCHMIDT, G. D. (1973): Resurrection of Southwellina Witenberg, 1932, with a description of Southwellina dimorpha sp. n. and a key to genera in Polymorphidae (Acanthocephala). - J. Parasit., 59: 299-305.

SHIRINOV, N. M. (1962a): Unusual helminth species of domestic waterfowl. - Trudy Az. N. I. Vet. Inst., 13: 120-124.

SHIRINOV, N. M. (1962b): Ten helminth species found at first in domestic geese and dues. - Trudy Az. N. I. Vet. Inst., 14: 75-80.

TSACHEVA, K. (1967): A contribution to investigation of helminth fauna of wild birds in Thrace. - Izv. Zool. Inst, with musium, BAN, 23: 175-181.

VAIDOVA, S. M. (1964): About fauna and ecology of Acanthocephalans (Acanthocephala) of birds in Azerbaijan (Lencoranskaya zone and Muganskaja steppe) (in Russian). - Izv. Akad. Nauk Azerb. SSR, Ser. biol., 2: 29-35.

Received: 10 July, 1990.

Dr. DIMITROVA, Z. M. Section of Zoology, Higher Institute of Zootechnics and Veterinary Medicine D. Blagoev str. 62. B-6000 Stara Zagora

BULGARIA

Dr. MURAI, É. Zoological Department, Hungarian Natural History Museum Baross utca 13. H-1088 Budapest HUNGARY

Dr. GENOV, T. Institute of Parasitology, Bulgarian Academy of Sciences Akad. G. Bonchev str., Bl. 25. B-l 113 Sofia

BULGARIA