Daphnia orthology gene groups with over-abundances compared to insects

Notes: Gene_Group documentation is at http://insects.eugenes.org/arthropods/; Gene_Class is a consensus functional annotation of genes in this group; Description is a consensus description of the genes; Groups with limited annotations (hypothetical) were excluded from this list. Daph column is gene count for Daphnia, * mark is for nDaph > Insects with significance p < 0.01 using chi-square test, others are groups with 2+ Daphnia genes for 1- 1 Insect genes. dPI column lists protein percent identity mean, and maximum in the group. iAve, iMax are average, maximum other (insect) gene counts for the group; marks of ^Aphid indicate overabundant group shared with Aphid

Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G1539 2 88 0.9 1 ABC_transporter ATP-binding cassette sub-family F member 1 ^Aphid ARP1_G1456 2 56 1 2 Abhydrolase conserved hypothetical protein ^Aphid ARP1_G360 15* 45/70 1.3 4 Abhydrolase lysosomal acid lipase ^Aphid ARP1_G2060 2 94 1 1 Acetyl coA- 4-Hydroxybutyrate CoA-transferase ARP1_G2713 2 100 0.9 1 acetylcholinesterase membrane anchor CutA homolog conserved hypothetical protein ARP1_G1507 2 69 1 1 acetylgalactosaminidase alpha-galactosidase/alpha-n-acetylgalactosaminidase ^Aphid ARP1_G187 4 25/30 2.8 3 actin binding beta chain spectrin ARP1_G236 20* 52/95 1.6 3 Acyl_transf conserved hypothetical protein ARP1_G1343 4 72/97 1.1 2 acyl-CoA binding peroxisomal 3,2-trans-enoyl-CoA ARP1_G331 8 81/100 1.8 2 acyl-CoA binding acyl-CoA-binding protein ARP1_G1582 2 36 1.2 2 acyl-CoA dehydrogenase short-chain specific acyl-CoA dehydrogenase ARP1_G1760 3 48/57 1 1 Acyltransferase 1-acylglycerol-3-phosphate acyltransferase ARP1_G1989 3 57/76 1 2 Adenylate cyclase adenylate cyclase ^Aphid ARP1_G2596 2 100 0.9 1 Adenylate cyclase conserved hypothetical protein ^Aphid ARP1_G1070 4 59/69 1.1 2 ADH_short short-chain dehydrogenase ARP1_G1638 5 74/91 1 2 ADH_short dehydrogenase/reductase SDR family member 4 ARP1_G1957 2 62 1.1 2 ADH_short dehydrogenase/reductase SDR family member 7 ARP1_G3060 2 99 1 1 ADH_short short-chain dehydrogenase ^Aphid ARP1_G3360 3 76/85 1.1 2 ADH_short short-chain dehydrogenase ARP1_G5899 2 39 0.9 1 ADH_short GA21392-PA ARP1_G6031 4* 57/63 0.5 4 ADH_short Carbonyl reductase ^Aphid ARP1_G732 3 58/62 1.1 2 ADH_short steroid dehydrogenase ARP1_G9451 5* 49/59 0 1 ADH_short Steroid dehydrogenase ARP1_G3110 2 61 0.8 1 Adiponectin receptor adiponectin receptor protein 2 ARP1_G4686 2 99 1.1 2 alanine-glyoxylate transaminase Alanine-glyoxylate aminotransferase ARP1_G2324 3 96/98 1 1 Alcohol dehydrogenase class 3 alcohol dehydrogenase class 3 ARP1_G4324 2 80 0.9 1 Aldedh aldehyde dehydrogenase 7 family ARP1_G944 4 89/93 1.1 2 Aldedh pyrroline-5-carboxylate dehydrogenase ARP1_G1087 2 98 1.3 2 allatostatin receptor allatostatin C receptor 2 CG13702-PB ARP1_G1055 3 64/69 1.2 2 Alpha_L_fucos plasma alpha-L-fucosidase ARP1_G1408 2 77 1.1 2 amidase amidase ARP1_G5562 2 28 0.9 1 Amidohydro_1 CyPIN guanine aminohydrolase family (cpin-1) ARP1_G1329 2 71 1 1 and derivative metabolic processaromatic-L-amino-acid decarboxylase ARP1_G947 4 60/80 0.8 1 Amino acid transporter proton-coupled amino acid transporter 1 ARP1_G4427 2 99 1 1 aminolevulinate synthase 5-aminolevulinic acid synthase ARP1_G12942 3* 53/55 0 1 ammonium transporter Ammonium transporter (ISS) ARP1_G3342 2 65 1 1 Ammonium_transp Rh-like protein ARP1_G1292 3 76/78 1 1 AMP-binding long-chain-fatty-acid coa ARP1_G399 12* 59/98 1.4 3 AMP-binding AMP dependent coa ligase ARP1_G793 4 48/60 1.5 3 AMP-binding amp dependent coa ligase ARP1_G538 3 72/78 1.1 2 AMP-binding long-chain-fatty-acid coa ligase ARP1_G3452 3 67/69 0.8 1 Ancient conserved domain ancient conserved domain protein 2 (cyclin m2) ARP1_G2783 2 83 1.1 2 Arfaptin islet cell autoantigen 1 ARP1_G1181 3 44/58 1 1 Arrestin conserved hypothetical protein ^Aphid ARP1_G222 16* 51/96 2.1 5 Astacin nas-14 ARP1_G8409 4* 72/76 0.3 2 Astacin zinc metalloproteinase dpy-31 ARP1_G804 2 na 1 1 ATP-binding cassette sub-family D memberATP-binding 2 cassette sub-family D member 1 ARP1_G1932 2 96 1 1 ATPase conserved hypothetical protein ^Aphid ARP1_G195 15* 59/89 2.2 4 ATPase abc transporter ARP1_G203 11* 60/83 2 3 ATPase ATP-binding cassette sub-family G member 4 ARP1_G2163 2 89 1.1 2 ATPase ATP-dependent Clp ATP-binding subunit clpX ARP1_G890 2 91 1 1 ATPase mitochondrial atp synthase b chain ^Aphid ARP1_G964 3 57/80 1.1 2 acetylhexosaminidase beta-N-acetylglucosaminidase ARP1_G2899 3 87/89 1 1 binding tetratricopeptide repeat protein 1 ARP1_G794 2 82 1 2 binding importin beta-2 ARP1_G2288 2 43 1 1 Biotin_lipoyl dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase ARP1_G870 5 58/72 1.1 2 bombesin receptor subtype-3 bombesin receptor subtype-3 ^Aphid ARP1_G2258 2 99 1.1 2 Brix domain brix domain-containing protein 2 ARP1_G4778 2 99 0.8 1 Calcitonin gene-related peptide-receptor componentcalcitonin proteingene-related peptide-receptor component protein ARP1_G5840 2 85 0.9 1 calcium channel dihydropyridine-sensitive l-type calcium channel ARP1_G1199 2 31 1.2 2 calcium ion binding fibrillin 2 ARP1_G4096 3 58/70 0.9 1 calcium ion binding nadph oxidase ARP1_G4617 2 36 1 1 calcium ion binding fibulin 1 and ARP1_G970 4 72/98 1.3 2 calcium ion binding calcyphosine-like protein ARP1_G889 3 50/78 1.1 2 calcium ion transport Na/Ca exchange protein

don gilbert Page 1 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G640 2 100 0.9 1 cAMP-dependent protein kinase regulator camp-dependent protein kinase type i-beta regulatory subunit ARP1_G152 20* 52/89 2.5 4 Carb_anhydrase Carbonic anhydrase 2 CG6906-PA ARP1_G441 15* 96/100 0.9 2 Carb_anhydrase carbonic anhydrase ARP1_G3593 2 57 1 1 Carb_anhydrase putative cytoplasmic carbonic anhydrase ARP1_G1682 2 35 1.1 2 carbohydrate metabolic process conserved hypothetical protein ARP1_G4691 2 91 1 1 carboxylic acid metabolic process Glutamate decarboxylase-like 1 ARP1_G630 2 na 1.1 2 caspase activation disulfide ARP1_G1803 2 44 1.2 2 catalytic isochorismatase domain-containing protein 2 ARP1_G3515 2 99 1 1 catalytic conserved hypothetical protein ARP1_G1018 2 45 1.3 2 Cation efflux protein cation efflux protein/ zinc transporter ^Aphid ARP1_G3858 2 98 1 1 CCAAT-box-binding transcription factor conserved hypothetical protein ARP1_G702 2 36 1.1 2 cell cycle arrest Short stop/Kakapo long isoform ARP1_G402 3 43/56 2 2 Chitin synthase Chitin synthase variant 1 ARP1_G1754 4 78/100 1.1 2 Chitin_bind conserved hypothetical protein ARP1_G3063 2 63 1 1 Chitin_bind conserved hypothetical protein ARP1_G647 19* 39/100 0.2 1 Chitin_bind prenylcysteine oxidase 1 ARP1_G831 9* 57/96 0.6 2 Chitin_bind peritrophic membrane chitin binding protein ARP1_G581 4 62/77 1.2 2 Chloride channel chloride channel protein 2 ARP1_G3158 6* 46/71 0.6 2 Chloride channel ca-activated chloride channel protein ARP1_G3355 2 na 1 1 cholesterol transporter putative cholesterol transporter BmStart1 ARP1_G1191 2 46 1 1 Choline_kinase choline/ethanolamine kinase ARP1_G2585 2 98 1 1 Chondroitin synthase chondroitin synthase ARP1_G3822 2 100 1 1 Chromatin accessibility complex protein 1histone-fold protein CHRAC subunit ARP1_G4566 2 80 1 1 Chromatin assembly chromatin assembly factor i P60 subunit ??Aphid/Daph ARP1_G5454 4 58/82 0.7 1 Chromatin assembly conserved hypothetical protein ARP1_G2475 2 61 1 2 Chromodomain heterochromatin protein 1 alpha ^Aphid ARP1_G1301 4 94/99 1 1 Chromosome region maintenance chromosome region maintenance protein 1/exportin ARP1_G5080 2 58 0.8 1 Cl_peroxidase_N presqualene diphosphate phosphatase ^Aphid ARP1_G2893 2 99 1 1 Cleavage and polyadenylation cleavage and polyadenylation specificity factor subunit 3 ??Aphid/Daph ARP1_G888 3 100/100 1 1 Coiled-coil domain conserved hypothetical protein ARP1_G4616 2 96 1 1 coupled to transmembrane m... ATP-binding cassette sub-family B member 10 ARP1_G245 12* 64/99 2.1 4 CRAL_TRIO CRAL/TRIO domain-containing protein ??Aphid/Daph ARP1_G407 4 51/66 1.4 2 CRAL_TRIO CRAL/TRIO domain-containing protein ARP1_G177 27* 48/83 1 2 CRAL_TRIO_N CRAL/TRIO domain-containing protein ARP1_G2956 2 91 1 1 Crossover junction endonuclease EME1 conserved hypothetical protein ARP1_G1749 3 53/68 0.8 1 Crust_neurohorm prepro ion transport-like peptide ARP1_G3715 2 100 1 1 Cuticle SGT1 protein homolog ecdysoneless ARP1_G49 82* 65/100 0.9 1 Cuticle Cuticular protein 146; RR-2 family ??Aphid/Daph ARP1_G88 39* 83/100 2.5 8 Cuticle pupal cuticle protein 78E ARP1_G2401 2 83 1 1 Cyclin g cyclin g ARP1_G769 2 95 1.3 2 Cysteine synthase Squamous cell carcinoma antigen ^Aphid ARP1_G2266 2 79 1 2 cytidine deaminase cytidine deaminase ARP1_G276 2 na 0.9 1 Cyt_P450 Cytochrome P450 18a1 (CYPXVIIIA1) ARP1_G219 13* 52/100 2.4 5 Cyt_P450 cytochrome P450 CYP15A1 ARP1_G5951 10* 63/89 0 0 Cyt_P450 Cytochrome P450 3A14 ARP1_G2399 2 na 1.1 2 Deadenylation factor EDEN-BP deadenylation factor EDEN-BP ARP1_G315 3 47/64 2 2 Dehalogen_hydro phospholipid-transporting atpase 1 ^Aphid ARP1_G7467 5* 87/99 0.3 1 DENN domain-containing protein 2B suppression of tumorigenicity 5 ARP1_G1509 2 44 1 1 DNA topoisomerase DNA topoisomerase 2-binding protein 1 ^Aphid ARP1_G1272 5 75/100 1 1 DNA topoisomerase II DNA topoisomerase/gyrase ARP1_G1127 2 71 1 1 DNA unwinding during replication High mobility group protein DSP1 ARP1_G2361 2 na 0.9 1 DNA_helicase ATP-dependent DNA helicase recQ ??Aphid/Daph ARP1_G5200 2 96 0.9 1 Dynactin 4 protein dynactin 4 protein ARP1_G878 8* 83/99 1 2 Dynein intermediate chain 3 dynein intermediate chain 2 ^Aphid ARP1_G1239 3 66/82 0.9 1 EF_hand_Ca_bd paramyosin ^Aphid ARP1_G4626 2 97 1 1 efhand supercoiling factor ARP1_G1054 3 59/75 1.4 2 electron transport peroxisomal N1-acetyl-spermine/spermidine oxidase ARP1_G2168 2 35 1.1 2 EMP70 transmembrane 9 superfamily protein member 3 ARP1_G347 9* 57/88 1.9 4 Endonuclease deoxyribonuclease I ARP1_G2051 2 37 1.1 2 spermatogenesis associated factor ARP1_G1729 2 na 1 1 epidermal growth factor receptor signalingpole ... hole CG2845-PC ARP1_G7361 6* 46/85 0.3 1 Epithelium-restricted ESE-1-related Ets factorconserved hypothetical protein ARP1_G1330 2 96 1.1 2 Eukaryotic translation initiation eukaryotic translation initiation factor 4 gamma ??Aphid/Daph ARP1_G4041 2 27 0.9 2 Eukaryotic translation initiation eukaryotic translation initiation factor 2 alpha kinase 1 ??Aphid/Daph ARP1_G961 5 65/100 1.2 2 exodeoxyribonuclease three prime repair exonuclease 1 ARP1_G10093 4* 78/86 0.1 2 exonuclease Rrp6 CG7292-PB ARP1_G1203 2 39 1.1 2 exonuclease Putative uncharacterized protein ARP1_G4926 2 98 0.9 1 exonuclease 3'-5' exonuclease ARP1_G2036 2 94 1.1 2 Exosome complex exonuclease RRP40 conserved hypothetical protein ARP1_G3223 2 90 1 2 extracellular ligand-gated ion channel acti...histamine-gated chloride channel subunit ARP1_G386 25* 36/99 0.2 1 F-box F-box only protein 21 ??Aphid/Daph

don gilbert Page 2 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G1645 4 68/90 1 2 FAA_hydrolase fumarylacetoacetate domain-containing protein 1 ARP1_G404 2 25 0.7 1 Fasciclin adhesion midline fasciclin CG3359-PM ARP1_G685 3 65/95 1 1 female receptivity possible integral membrane efflux protein efpa ARP1_G614 2 59 1.2 2 ferrous iron binding Ferritin 2 light chain homologue CG1469-PC ARP1_G1558 3 92/94 1 1 Fizzy-related protein retina aberrant in pattern CG3000-PA ARP1_G1757 3 80/84 1 1 FKBP_C FK506-binding protein FKBP59 CG4535-PA ARP1_G1707 2 75 1 2 Flotillin flotillin-1 ARP1_G42 89* 41/99 1 2 fucosyltransferase alpha(1,3)fucosyltransferase ARP1_G3059 2 99 1 1 Fused toes protein homolog Fused toes protein homolog ARP1_G1123 4 75/85 1.1 2 G-protein coupled receptor putative allatostatin receptor 1 ARP1_G1973 3 77/84 1 1 G-protein coupled receptor dopamine receptor type D2 ARP1_G3269 2 81 1 2 G-protein coupled receptor ecdysis triggering hormone receptor subtype-B ARP1_G6191 2 47 0.7 1 G-protein coupled receptor leucine rich repeat G protein coupled receptor ARP1_G4146 3 74/82 0.8 2 G-protein coupled receptor protein signalin...g-protein coupled receptor ARP1_G774 2 na 0.9 1 GABA receptor GABA receptor subunit ^Aphid ARP1_G607 7 57/98 1.1 2 Galactosyltransferase beta-1,4-galactosyltransferase ^Aphid ARP1_G2213 3 94/98 1 1 Galactosyltransferase beta 1,3-galactosyltransferase ARP1_G2383 2 57 1.2 2 Galactosyltransferase beta-1,3-galactosyltransferase ARP1_G3385 7* 47/76 0.6 6 Galactosyltransferase Lactosylceramide galactosyltransferase ARP1_G7731 7* 42/96 0.1 1 Galactosyltransferase Lactosylceramide 4-alpha-galactosyltransferase ARP1_G328 13* 52/88 1.4 3 Galactosyltransferase Lactosylceramide 4-alpha-galactosyltransferase ARP1_G1111 3 68/79 1.1 2 gamma-aminobutyric acid metabolic process4-aminobutyrate aminotransferase ARP1_G2355 2 83 1 1 gap junction innexin 2 CG4590-PA ARP1_G5653 2 57 0.7 1 gap junction innexin 3 CG1448-PA ARP1_G9494 3* 84/100 0.2 3 Globin hemoglobin T1 polymer ^Aphid ARP1_G5340 2 64 0.9 1 glucose-regulated protein Heat shock protein cognate 5 CG8542-PA ARP1_G242 14* 39/71 2.1 8 glucuronosyl glucosyl/glucuronosyl transferases ARP1_G713 2 67 0.8 1 Glutamate-gated chloride channel glutamate-gated chloride channel ARP1_G1053 4 73/98 1 1 Glutamyl hydrolase gamma-glutamyl hydrolase ^Aphid ARP1_G246 9* 57/92 1.8 5 glutathione transferase Glutathione S-transferase ARP1_G9149 6* 79/92 0 0 glutathione transferase ARP1_G8533 5* 46/64 0 1 glyceroaquaporin Aquaporin 3 ARP1_G956 3 88/93 0.9 1 glycerol-3osphate dehydrogenase lethal (2) k05713 CG8256-PA ARP1_G2118 2 96 1.1 2 glycine dehydrogenase glycine dehydrogenase ARP1_G1578 7* 57/95 0.8 2 Glyco_hydro_16 Gram-negative bacteria binding protein 1 CG6895-PA ARP1_G616 2 64 1 2 Glyco_hydro_47 mannosyl-oligosaccharide alpha-1,2-mannosidase ARP1_G2047 2 69 1.1 2 Glyco_hydro-type_carb-bd_sub aldose 1-epimerase ^Aphid ARP1_G2290 2 98 1.1 2 Glyco_transf_22 dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1 ARP1_G951 2 43 1.4 2 glycoprotein catabolic process l-asparaginase ARP1_G81 16* 61/88 4.6 11 GMC_OxRdtase_N glucose dehydrogenase ^Aphid ARP1_G2030 2 25 0.9 1 golgi apparatus protein 1 MG-160 ^Aphid ARP1_G1337 2 90 1 1 GTP binding GTP-binding protein ARP1_G3057 3 86/97 1 1 GTP binding GTP-binding protein Di-Ras2 ARP1_G2570 2 na 1 1 GTP binding GTP-binding protein era ARP1_G5544 2 70 0.7 1 GTP binding rho-like CG9366-PA ??Aphid/Daph ARP1_G934 2 94 1 1 GTP binding gtp-binding protein sar1 ARP1_G797 4 57/74 1.4 2 Guanylate_cyc Guanylyl cyclase at 89Da CG14885-PB ARP1_G1065 5 55/67 0.9 1 Haem_peroxidase_animal GA19195-PA ^Aphid ARP1_G4925 4* 59/91 0.6 4 HAT family dimerisation domain predicted protein ^Aphid ARP1_G247 13* 83/99 1.7 4 HATPase_c heat shock protein 90 ARP1_G3980 2 100 0.8 1 HCaRG conserved hypothetical protein ARP1_G4910 2 68 0.8 1 Hcp beta-lactamase-like protein beta-lactamase hcpA ARP1_G1721 2 97 1 1 heat shock protein binding f string protein CG6395-PC ARP1_G2170 3 90/98 1 1 heat shock protein binding conserved hypothetical protein ARP1_G2310 2 28 1.1 2 heat shock protein binding conserved hypothetical protein ARP1_G3685 6* 53/98 0.5 1 helicase senataxin splicing endonuclease positive effector sen1 ARP1_G2743 2 64 1 1 Hemomucin adipocyte plasma membrane-associated protein ARP1_G1606 3 73/79 1 1 HIF prolyl hydroxylase HIF prolyl hydroxylase CG31543-PC ARP1_G1308 2 93 1.1 2 Histidine_acid_Pase Acid phosphatase 1 CG7899-PA ^Aphid ARP1_G41 23* 100/100 7 23 Histone histone H3 type 2 ARP1_G1589 4 57/90 1 1 homophilic cell adhesion Cad88C CG3389-PA ARP1_G928 3 67/72 1.2 3 HSP20 lethal(2)essential for life protein ARP1_G4543 2 92 1 1 hydrolase nudix hydrolase 6 ARP1_G3817 2 25 0.8 1 hydrolase Ectonucleoside triphosphate diphosphohydrolase 1 ARP1_G901 4 47/60 1.3 2 hydrolase valacyclovir hydrolase ARP1_G1407 3 65/71 1.3 2 hydrolase valacyclovir hydrolase ARP1_G5881 3 79/99 0.8 1 imaginal disc-derived leg joint morphogenesisfour-jointed protein ARP1_G3850 2 60 0.9 2 Immunoglobulin defective proboscis extension response ^Aphid ARP1_G1649 2 100 1.1 2 Immunoglobulin-binding Two A-associated protein of 42kDa CG31852-PA ^Aphid ARP1_G2914 3 100/100 0.9 1 inositol phosphatase myo inositol monophosphatase ??Aphid/Daph ARP1_G2934 2 95 1 1 inositol phosphatase myo inositol monophosphatase

don gilbert Page 3 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G1246 3 85/92 1.1 2 integrin-mediated signaling pathway integrin alpha-ps ARP1_G2071 2 52 1.1 2 intracellular signaling cascade rac GTPase-activating protein ARP1_G1716 3 98/99 1.1 2 intraflagellar transport Intraflagellar transport 122 homolog (Chlamydomonas) ARP1_G756 2 66 1.1 2 Ion_trans_2 small conductance calcium-activated potassium channel CG10706-PB ARP1_G1354 2 92 1.1 2 ionotropic glutamate receptor Glutamate NMDA receptor subunit variant NR2-a ??Aphid/Daph ARP1_G2533 2 61 1 1 Isoprene biosynthesis candidate tumor suppressor protein ^Aphid ARP1_G2391 3 54/54 1.2 2 Kinesin kinesin-like protein KIF1B ARP1_G5884 2 na 1 1 Kinesin GA20615-PA ARP1_G783 2 60 1.1 2 kinesin kinesin-like protein Klp10A ^Aphid ARP1_G2436 7* 36/53 0.4 2 Kunitz_BPTI CG6784 CG6784-PB ARP1_G1197 2 98 1 2 Kurtz arrestin-like protein ArrK beta-arrestin 1 ARP1_G862 2 80 1.1 2 kynurenine aminotransferase kynurenine-oxoglutarate transaminase 1 ^Aphid ARP1_G6268 2 99 0.8 1 Lactamase_B GA21555-PA ARP1_G4515 2 41 0.9 2 Leucine rich protein leucine rich protein ARP1_G798 2 77 1.4 2 LIM beadex/dLMO protein ARP1_G330 13* 45/77 1.6 3 lipid metabolic process pancreatic triacylglycerol lipase ARP1_G1108 3 42/52 1.3 2 Lipocalin Neural Lazarillo CG33126-PA ARP1_G1081 7 57/70 1 1 lipoprotein receptor low-density lipoprotein receptor (ldl), cueball ARP1_G423 9* 67/99 1.3 2 lipoprotein receptor low-density lipoprotein receptor (ldl), yolkless ARP1_G1195 2 52 1.3 2 Lysosomal beta A mannosidase beta-mannosidase ARP1_G1367 3 41/64 1.3 3 lysosomal membrane sialoglycoprotein sensory neuron membrane protein-1 ARP1_G1617 5 58/89 0.9 1 Macroglobulin Macroglobulin complement-related CG7586-PA ARP1_G2598 2 58 1 1 Mannosyltransferase Beta 1,4-mannosyltransferase egh ??Aphid/Daph ARP1_G69 73* 37/100 0.3 4 MAPP family RE34508p ARP1_G320 31* 44/93 0 1 MATH conserved hypothetical protein ARP1_G5896 2 93 0.9 1 Mechanosensory abnormality protein 2 AGAP003352-PA ARP1_G919 2 76 1.2 2 Melastatin 2 conserved hypothetical protein ARP1_G2731 2 68 1 1 membrane conserved hypothetical protein ARP1_G7170 5* 68/81 0.4 2 membrane microsomal dipeptidase ARP1_G1652 3 53/64 1 2 metalloexopeptidase xaa-pro ARP1_G2238 2 87 1 1 metalloexopeptidase proliferation-associated 2g4 (pa2g4/ebp1) ARP1_G92 17* 44/91 3.9 13 Metalloprotease protease m1 zinc metalloprotease ^Aphid ARP1_G287 13* 46/88 1.5 2 Methuselah methuselah-like Aphid/Daph ARP1_G786 3 90/94 1.2 2 methylenetetrahydrofolate methylenetetrahydrofolate dehydrogenase ARP1_G7796 2 na 0.7 1 methylenetetrahydrofolate 5,10-methylenetetrahydrofolate reductase ARP1_G1240 2 100 1 1 methylglutaryl coenzyme A synthase hydroxymethylglutaryl-CoA synthase 1 ARP1_G2809 2 100 1 1 methyltransferase N6-adenosine-methyltransferase IME4 ARP1_G2873 2 100 1 1 methyltransferase protein arginine n-methyltransferase 1 ARP1_G4412 2 63 1 1 methyltransferase Nasonia vitripennis hypothetical protein ARP1_G740 8* 61/100 0 1 methyltransferase Nasonia vitripennis hypothetical protein ARP1_G1180 3 79/88 1 2 methyltransferase N6-adenosine-methyltransferase IME4 ARP1_G1864 2 56 1 1 Methyltransferase histone-lysine N-methyltransferase SETDB1 ^Aphid ARP1_G678 2 na 1.2 2 Methyltransferase histone-lysine n-methyltransferase ^Aphid ARP1_G1997 2 50 1.1 2 Microtubule associated Microtubule associated protein 1 light chain 3 beta ??Aphid/Daph ARP1_G495 4 90/96 0.9 1 Microtubule associated microtubule associated protein RP/EB family member 3 ??Aphid/Daph ARP1_G1497 2 70 1 2 mitochondrial inner membrane folate carrier protein ARP1_G2066 2 40 1.1 2 Mitochondrial inner membrane mitochondrial inner membrane protein ARP1_G1918 2 53 1 1 mitochondrial precursor conserved hypothetical protein ARP1_G2184 2 51 1.1 2 Mitochondrial succinate dehydrogenase mitochondrial succinate dehydrogenase cytochrome b subunit ARP1_G2128 2 54 1 2 Mitochondrial transcription mitochondrial transcription factor A ARP1_G2635 2 82 1 2 Molybdopterin molybdopterin synthase large subunit ARP1_G1204 5 92/97 1 1 mRNA cleavage pre-mRNA cleavage complex II protein Clp1 ??Aphid/Daph ARP1_G1345 2 88 0.8 2 mRNA cleavage musashi/mRNA cleavage and polyadenylation factor I complex subunit HRP1 ARP1_G128 5 56/98 3.9 4 mRNA-splicing factor pre-mRNA-splicing factor ATP-dependent RNA helicase prp22 ^Aphid ARP1_G1893 2 88 1.1 2 mRNA-splicing factor step ii splicing factor slu7 ARP1_G1251 2 99 1.3 2 multicellular organismal development gooseberry-neuro CG2692-PA ARP1_G414 3 68/96 1.7 2 myosin myosin i ??Aphid/Daph ARP1_G848 2 36 1.4 2 N-acetyltransferase heparan-alpha-glucosaminide N-acetyltransferase ^Aphid ARP1_G181 13* 56/90 2.6 4 NaKCl_cotransptS bumetanide-sensitive Na-K-Cl cotransport protein ^Aphid ARP1_G2797 2 na 1.1 2 negative regulation of cyclin-dependent pro...radical sam proteins ARP1_G12993 3* 91/94 0 0 negative regulation of transcription ARP1_G7400 9* 52/88 0 1 neprilysin ARP1_G2651 2 79 0.9 1 Neur_chan_memb histamine-gated chloride channel subunit ARP1_G4490 2 80 0.9 1 Neur_chan_memb nicotinic acetylcholine receptor subunit alpha2 ARP1_G2113 2 97 0.9 1 NICE-3 NICE-3 family member ARP1_G1740 3 91/96 1 1 Nicotinamide adenylyltransferase nicotinamide mononucleotide adenylyltransferase 1 ARP1_G393 3 73/80 1.5 2 Nmda receptor glutamate-binding nmda receptor glutamate-binding chain ARP1_G5945 8* 44/83 0.3 2 Notch signaling pathway CG3491 CG3491-PA ARP1_G3821 2 76 0.9 1 Nuclear valosin peroxisome assembly factor-2 (peroxisomal-type atpase 1) ARP1_G1471 2 100 0.9 1 nucleic acid binding conserved hypothetical protein ARP1_G3756 2 na 1 2 nucleic acid binding conserved hypothetical protein

don gilbert Page 4 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G5382 2 79 0.8 1 nucleic acid binding conserved hypothetical protein ARP1_G608 4 70/95 1.5 2 nucleic acid binding transcriptional regulator ATRX (X-linked helicase II) ARP1_G2171 2 90 1 1 Nucleoporin nuclear pore complex protein nup154 ^Aphid ARP1_G6389 2 100 0.8 1 Nucleoporin nucleoporin NUP53 ??Aphid/Daph ARP1_G864 3 73/79 1.2 2 nucleoside cotransporter sodium/nucleoside cotransporter ARP1_G1315 5 97/100 0.9 1 Nucleoside diphosphate kinase nucleoside-diphosphate kinase NBR-A ^Aphid ARP1_G3671 2 83 0.9 1 nucleoside triphosphatase AGAP005176-PA ARP1_G629 5 86/99 1.5 2 nucleoside triphosphatase vesicular-fusion protein nsf ARP1_G17 25* 100/100 10.1 21 nucleosome assembly Histone H4 replacement CG3379-PC ARP1_G635 3 78/80 1.1 2 Nucleotidyltransferase PAPS synthetase CG8363-PA ^Aphid ARP1_G364 4 58/82 1.4 2 oligopeptide transporter oligopeptide transporter ^Aphid ARP1_G145 21* 59/100 2.7 6 Opsin long wavelength sensitive opsin 1 ARP1_G2260 2 50 1 1 Opsin ultraviolet-sensitive opsin ^Aphid ARP1_G3992 2 49 0.7 1 Opsin ultraviolet-sensitive opsin ARP1_G1147 2 71 1.4 2 Opsin 5-hydroxytryptamine receptor 2, Rhodopsin ARP1_G355 6 43/78 1.9 3 organic cation transporter organic cation transporter ^Aphid ARP1_G7296 2 97 0.8 1 Origin recognition complex subunit 6 origin recognition complex subunit 6 ARP1_G1351 5 81/99 1 1 Otopetrin otopetrin ARP1_G4665 2 61 1 1 Otopetrin otopetrin ARP1_G11188 4* 73/100 0 1 oxidoreductase Aldo-keto reductase ARP1_G1756 2 94 0.9 1 oxidoreductase electron transfer flavoprotein-ubiquinone oxidoreductase ARP1_G1785 7* 69/86 0.7 1 oxidoreductase Ferritin 3 heavy chain homologue CG4349-PA ARP1_G2405 5 60/69 1 2 oxidoreductase conserved hypothetical protein ARP1_G11202 3* 95/97 0 0 Oxysterol_BP Oxysterol-binding protein ARP1_G1039 2 76 0.9 1 P_proprotein proprotein convertase subtilisin/kexin type 4 ARP1_G1217 4 66/69 1 1 PAP2 phosphatidate phosphatase ARP1_G10099 4* 52/63 0 1 Peptidase Adult male pituitary gland cDNA ARP1_G249 11* 50/97 2.2 5 Peptidase putative A-like ARP1_G580 2 44 1.1 2 Peptidase isoform 1B long tail-2 ARP1_G861 7 61/80 1.1 2 Peptidase zinc carboxypeptidase ARP1_G585 6 57/80 1.6 3 Peptidase zinc carboxypeptidase ARP1_G8513 7* 57/100 0 1 Peptidase ARP1_G2222 12* 38/100 0.2 3 Peptidase_C48 ^Aphid ARP1_G4955 4* 33/41 0.7 5 Peptidase_C48 predicted protein ^Aphid ARP1_G1020 2 30 1 1 peptidyl-histidine phosphorylation Pyruvate dehydrogenase kinase CG8808-PA ARP1_G1057 9* 64/86 0.8 1 permease ATP dependent transmembrane transporter protein ARP1_G1342 3 91/97 1.1 2 peroxidase dual oxidase 1 ARP1_G1914 2 na 1.1 2 peroxidase oxidase/peroxidase ARP1_G202 19* 50/85 1.9 4 peroxidase Peroxidase 9 (Fragment). ARP1_G2758 2 45 1.1 2 peroxidase oxidase/peroxidase ARP1_G5814 2 91 1 1 peroxidase oxidase/peroxidase ARP1_G1596 3 87/89 1 1 Peroxiredoxin peroxiredoxin 5 ARP1_G1290 2 na 0.8 1 Phosphatase and tensin protein Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ARP1_G1225 3 56/100 1 1 phosphatidylinositol 3-kinase phosphatidylinositol 3-kinase catalytic subunit alpha ARP1_G1624 2 71 1.1 2 Phosphatidylinositol 3-kinase phosphatidylinositol 3-kinase regulatory subunit ^Aphid ARP1_G180 17* 53/99 2.4 17 Phospholipase F57G4.9 ^Aphid ARP1_G2116 2 59 1 1 Phospholipase phospholipase c gamma ^Aphid ARP1_G2430 2 46 1 1 phospholipase A2 GXIVsPLA2 CG17035-PA ARP1_G3465 2 55 1 1 phospholipid metabolic process secretory Phospholipase A2 ARP1_G484 3 72/84 1.5 3 Phospholipid-hydroperoxide glutathione peroxidasephospholipid-hydroperoxide glutathione peroxidase ARP1_G1604 2 99 1 1 Phosphoprotein conserved hypothetical protein ^Aphid ARP1_G1012 2 89 0.9 1 Pkinase Protein kinase related to protein kinase N CG2049-PB ARP1_G12939 3* 67/73 0 0 Pkinase ARP1_G396 27* 51/100 0 1 Pkinase_Tyr VEGFR-A splice form A ARP1_G6687 2 na 0.9 1 Pkinase_Tyr tyrosine protein kinase with ig domain ARP1_G9121 6* 60/99 0 1 Pkinase_Tyr FMS-like tyrosine kinase 1 ARP1_G1786 2 56 1.2 2 Pmp22 peroxisomal membrane protein pmp22 peroxisomal membrane protein ARP1_G4679 3 95/99 1 2 Polymerase III polypeptide conserved hypothetical protein ARP1_G1546 4 56/63 1 1 polyprenyl_synt trans-prenyltransferase ARP1_G857 3 95/96 1.3 3 positive regulation of protein kinase conserved hypothetical protein ARP1_G5602 2 40 0.9 2 potassium channel GA21275-PA ARP1_G2409 2 82 1.2 2 PQ-loop conserved hypothetical protein ARP1_G1472 2 98 1.2 2 protease regulatory subunit 26S protease regulatory subunit 6b ARP1_G1067 2 54 0.9 2 proteasome activator adhesion regulating molecule 1 ARP1_G2773 2 71 0.9 1 protein amino acid phosphorylation epidermal growth factor receptor ARP1_G3157 2 74 0.9 1 protein amino acid phosphorylation conserved hypothetical protein ARP1_G2333 2 83 1 1 Protein bicaudal C homolog 1 bicaudal-c ARP1_G10076 5* 82/100 0 1 protein binding CG9791 CG9791-PB ARP1_G2286 2 100 1 1 protein binding inner membrane protein COX18 ARP1_G2451 14* 34/46 0 1 protein binding Tribolium castaneum hypothetical LOC664274 ARP1_G2642 3 83/89 1 1 protein binding conserved hypothetical protein

don gilbert Page 5 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G3032 2 85 1 1 protein binding conserved hypothetical protein ARP1_G4931 6* 79/100 0.5 1 protein binding conserved hypothetical protein ARP1_G8519 7* 78/97 0 0 protein binding ARP1_G3352 2 97 1 1 Protein KTI12 homolog cyclohex-1-ene-1-carboxyl-CoA hydratase ARP1_G931 6 71/76 1.1 2 protein metabolic process prolyl 4-hydroxylase alpha subunit 1 ARP1_G4292 2 86 1 1 Protein Swiss cheese neuropathy target esterase/swiss cheese ARP1_G1371 3 69/76 1.2 2 protein transporter importin beta-4 ARP1_G4712 3 80/83 1 1 protein transporter importin alpha ARP1_G869 3 55/65 0.8 1 protein-methionine-S-oxide reductase methionine-R-sulfoxide reductase ARP1_G2981 2 na 1 2 conserved hypothetical protein ARP1_G6875 2 100 0.7 1 pseudouridine synthase conserved hypothetical protein ARP1_G10019 5* 89/100 0 1 regulation of GTPase centaurin-alpha 2 ARP1_G3311 4 89/96 1 1 regulation of Rab GTPase conserved hypothetical protein ARP1_G2326 2 91 1 1 regulation of small GTPase mediated signalguanine ... nucleotide exchange factor ARP1_G2760 2 40 1.1 2 Replication protein insect replication protein a ARP1_G1107 7 77/91 1 1 RGS regulator of g protein signaling ARP1_G2062 3 69/98 1 1 Ribosomal ribokinase ARP1_G1466 3 100/100 1 1 Ribosomal 40S ribosomal protein S9 ARP1_G2227 2 98 0.9 1 Ribosomal Ribosomal protein L3 ARP1_G2925 2 68 1 1 Ribosomal mitochondrial ribosomal protein S18B ARP1_G1227 2 99 1.1 2 Ribosomal 40S ribosomal protein S2 ARP1_G2525 2 84 1 1 Ribosomal mitochondrial ribosomal protein S35 CG2101-PA ARP1_G3483 2 92 1 1 Ribosomal mitochondrial 60S ribosomal protein L7/L12 ARP1_G2993 2 100 1 1 Ribosomal mitochondrial ribosomal protein S29 CG3633-PA ARP1_G682 3 72/99 1 2 Rieske rieske-domain protein Neverland ARP1_G575 8 61/81 1.3 3 RNA binding suppressor of ty ARP1_G1847 4 80/87 1 1 RNA guanylyltransferase mRNA-capping- CG1810-PA ARP1_G2754 2 92 1 1 RNA helicase DEAH (Asp-Glu-Ala-His) box polypeptide 36 ARP1_G2666 2 92 1.1 2 RNA polymerase DNA-directed RNA polymerase ??Aphid/Daph ARP1_G4327 2 88 1 1 RNA polymerase RNA polymerase II mediator complex subunit 10 ??Aphid/Daph ARP1_G196 15* 59/99 2 4 RNA polymerase DNA-directed RNA polymerase II largest subunit ^Aphid ARP1_G1968 2 82 1.1 2 RNA polymerase general transcription factor 3C polypeptide 3 ARP1_G4523 3 83/98 0.9 1 RNA polymerase DNA-directed RNA polymerase III subunit F ARP1_G3272 2 100 1.1 2 RNA terminal phosphate cyclase domain 1RNA 3'-terminal phosphate cyclase ARP1_G3210 2 31 1 1 RNA_helicase ATP-dependent RNA helicase A ARP1_G5442 2 94 0.9 1 RNA_helicase dead box atp-dependent rna helicase ??Aphid/Daph ARP1_G2543 3 48/64 1 1 RNA_helicase Dicer-1 ??Aphid/Daph ARP1_G1455 2 97 1 1 RNA-binding small bristles CG1664-PA ^Aphid ARP1_G684 2 76 1.3 2 RNA-binding RNA-binding protein ^Aphid ARP1_G3176 2 33 0.9 1 RPE65 beta-carotene dioxygenase ARP1_G1298 3 88/99 1.1 2 SART-1 U4/U6.U5 tri-snRNP-associated protein 1 ARP1_G1802 4 58/72 1 1 scavenger receptor scavenger receptor acting in neural tissue ??Aphid/Daph ARP1_G412 6 49/81 1.7 3 SCP conserved hypothetical protein ARP1_G4392 2 100 1 1 Sec61 protein translocation complex beta-subunitprotein translocation complex beta subunit ARP1_G4603 2 64 1 1 Secretory carrier membrane protein secretory carrier-associated membrane protein (scamp) ARP1_G2284 2 32 1.1 2 sequence-specific DNA binding DNA-binding protein D-ELG ARP1_G4541 2 97 1 1 sequence-specific DNA binding WD repeat and HMG-box DNA-binding protein 1 ARP1_G8696 4* 88/98 0.2 1 sequence-specific DNA binding CG15828 CG15828-PA ARP1_G8784 6* 53/98 0 1 sequence-specific DNA binding ARP1_G1139 2 28 1.3 2 Ser_thr_pkinase Unc-89 CG33519-PB ARP1_G5823 4 98/99 0.7 1 Ser_thr_pkinase mitosis inhibitor protein kinase ??Aphid/Daph ARP1_G3668 2 74 1 2 Ser_thr_pkinase Serine/threonine-protein kinase 6 (Aurora-A) (Aurora/IPL1-related kinase 1) ARP1_G899 3 72/78 1.1 2 Ser_thr_pkinase serine/threonine-protein kinase polo ^Aphid ARP1_G1183 2 99 1 1 Ser_thr_pkinase serine/threonine-protein kinase SRPK2 ARP1_G2202 2 100 1 1 Ser_thr_pkinase serine/threonine-protein kinase rio1 (rio kinase 1) ARP1_G2231 2 97 1 1 Ser_thr_pkinase serine/threonine-protein kinase OSR1 ARP1_G2352 2 56 0.9 1 Ser_thr_pkinase mitogen activated protein kinase kinase kinase ??Aphid/Daph ARP1_G2946 2 82 1 1 Ser_thr_pkinase mitogen activated protein kinase kinase 4 MAPKK4 ARP1_G4619 8* 55/87 0.4 2 serine esterase carboxylesterase ARP1_G6982 2 79 0.7 1 serine esterase ace1 type acetylcholinesterase ARP1_G2331 4 66/93 1 1 trypsin-like serine protease ARP1_G2548 2 82 1 1 serine protease CLIP-domain serine protease subfamily D ??Aphid/Daph ARP1_G4231 2 63 0.9 1 serine protease CLIP-domain serine protease subfamily A ??Aphid/Daph ARP1_G2199 2 61 1 1 serine protease Reeler conserved hypothetical protein ARP1_G358 20* 36/79 0.9 2 serine protease Reeler serine protease ^Aphid ARP1_G936 2 92 1.1 2 serine endopeptidase scavenger receptor class a-like and serine protease ARP1_G7395 6* 87/100 0.3 2 serine endopeptidase inhibitor CG16704 CG16704-PA ARP1_G191 2 40 1.3 2 Serpin serine protease inhibitor 4 ^Aphid ARP1_G2104 2 80 1.1 2 signal recognition signal recognition particle 19 kDa protein ^Aphid ARP1_G7086 2 68 0.7 1 signal transducer hypoxia-inducible factor 1 alpha ARP1_G5630 2 91 0.8 1 SNF high-affinity serotonin transporter

don gilbert Page 6 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G1770 4 71/81 0.9 1 snRNA processing integrator complex subunit 2 ARP1_G3284 2 86 1 1 snRNA processing Integrator complex subunit 6 ARP1_G2077 3 94/98 1 2 snRNA processing Integrator complex subunit 7 (Int7) ARP1_G452 3 54/56 1.9 2 Sodium bicarbonate cotransporter Na-driven anion exchanger 1 CG4675-PA ARP1_G3861 2 79 0.9 1 Sodium-bile acid cotransporter sodium-bile acid cotransporter ARP1_G11103 4* 64/78 0 0 sodium-dependent phosphate transporter Slc34a2a protein ARP1_G1790 4 71/99 1 1 sodium:hydrogen antiporter alkali metal ion/proton exchanger 3 ARP1_G12867 3* 53/89 0 1 Solute carrier family 17 member 5 Sialin ARP1_G2953 2 93 0.9 1 Solute carrier family 25 member 37 mitochondrial rna splicing protein ARP1_G3274 3 40/45 0.7 1 Somatomedin_B Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ARP1_G10029 5* 74/95 0 0 Sphingomyelin Sphingomyelin phosphodiesterase D 5 precursor ARP1_G1410 2 30 1 1 Sphingomyelin sphingomyelin phosphodiesterase ARP1_G3937 2 45 0.9 2 Ss-alanyl conjugating enzyme beta-alanyl conjugating enzyme ARP1_G266 5 68/78 1.8 3 stearoyl-CoA desaturase delta(9)-desaturase ARP1_G3446 2 88 0.9 1 Stress-activated protein kinase Stress-activated protein kinase JNK ARP1_G1333 4 78/87 1.1 2 Stromal antigen stromal antigen ARP1_G2792 2 79 1.1 2 Structural maint. of chromosomes structural maintenance of chromosomes protein 1A ARP1_G1616 2 79 1 1 Structural maint. of chromosomes structural maintenance of chromosomes 6 smc6 ^Aphid ARP1_G140 23* 50/100 2.7 4 Sulfotransferase sulfotransferase (sult) ARP1_G929 2 65 0.8 1 Superoxide dismutase [Cu-Zn] extracellular Cu/Zn superoxide dismutase ARP1_G1098 2 55 1 1 TB2_DP1_HVA22 conserved hypothetical protein ARP1_G280 16* 50/100 1 8 Tc1 transporase ARP1_G2078 4 49/72 0.8 1 Tetraspannin conserved hypothetical protein ARP1_G2374 2 100 1.1 2 Thiamin pyrophosphokinase 1 thiamin pyrophosphokinase 1 ARP1_G1887 2 79 1 1 THO complex subunit 2 THO complex subunit 2 (Tho2) ARP1_G1110 4 92/96 0.9 1 thymidylate kinase Deoxythymidylate kinase (thymidylate kinase) ARP1_G1536 2 96 1.2 2 thymidylate synthase bifunctional dihydrofolate reductase-thymidylate synthase ARP1_G4557 2 na 1 1 timeless timeout/timeless-2 ARP1_G586 9* 47/70 0.7 1 timeless timeless protein ARP1_G5357 2 89 0.8 1 TmcA TmcA-like protein (Fragment). ARP1_G779 2 44 1.3 2 TPR_region Nasonia vitripennis hypothetical protein ^Aphid ARP1_G4640 2 70 1.1 2 TRAF interacting protein conserved hypothetical protein ARP1_G4399 2 99 1 2 Transcription initiation Transcription initiation factor TFIID subunit 11 ??Aphid/Daph ARP1_G3525 2 83 1 1 Transcription initiation transcription initiation factor IIE subunit beta ??Aphid/Daph ARP1_G2162 2 na 1.1 2 Transcription regulation t-box transcription factor tbx20 ^Aphid ARP1_G716 20* 37/97 0 1 transcriptional regulator ycf27 predicted protein ARP1_G547 11* 57/96 0.9 1 transferase glucosyl/glucuronosyl transferases ARP1_G1520 3 58/65 1.1 2 transferring glycosyl... Protein O-mannosyltransferase 2 CG12311-PA ARP1_G3299 2 23 1 1 Transforming growth factor beta receptor-associatedconserved proteinhypothetical 1 protein ARP1_G2014 7* 72/99 0.5 2 Transglut_core transglutaminase ARP1_G1291 2 33 1.2 2 Transmembrane conserved hypothetical protein ^Aphid ARP1_G920 2 33 1.4 2 Transmembrane conserved hypothetical protein ^Aphid ARP1_G2564 2 90 1 1 transport lung seven transmembrane receptor ARP1_G4678 3 49/53 1 1 transport conserved hypothetical protein ARP1_G4708 4 70/78 0.9 1 transport sodium-dependent phosphate transporter ARP1_G1063 4 69/73 1 1 transport conserved hypothetical protein ARP1_G633 2 61 1.1 2 Trehalase alpha,alpha-trehalase ^Aphid ARP1_G1465 3 58/64 1 1 Trehalose_PPase Trehalose-6-phosphate synthase 1 CG4104-PA ^Aphid ARP1_G5771 2 88 0.9 1 Trehalose_rcpt Gustatory receptor for trehalose ??Aphid/Daph ARP1_G712 3 92/94 1.5 2 tricarboxylic acid cycle succinate dehydrogenase flavoprotein subunit ARP1_G2423 2 83 1.1 2 tricarboxylic acid cycle Ecdysone-inducible gene L3 CG10160-PA ARP1_G2826 2 88 0.9 1 Trimethylguanosine synthase homolog prip interacting protein. pimt ^Aphid ARP1_G781 2 na 0.9 2 triphosphate receptor Inositol 1,4,5,-tris-phosphate receptor CG1063-PA ARP1_G558 11* 90/100 1 1 tRNA ribosyltransferase queuine tRNA-ribosyltransferase ARP1_G2592 2 85 1 1 tRNA synthetase Cysteinyl-tRNA synthetase CG8431-PA ARP1_G1663 2 90 1 1 tRNA synthetase isoleucyl tRNA synthetase ARP1_G918 2 93 1 1 tRNA synthetase threonyl-tRNA synthetase ^Aphid ARP1_G4649 3 67/80 1 1 trypsin GA19914-PA ARP1_G336 17* 50/96 1.1 10 Trypsin microtubule binding protein ARP1_G549 23* 42/99 0 0 Trypsin ARP1_G2507 2 70 1 1 TSP_1 Sema-5c CG5661-PA ARP1_G56 72* 79/100 1 2 Tubulin-folding beta-tubulin cofactor d ARP1_G2020 2 100 1 1 Ubiquinone NADH:ubiquinone dehydrogenase ^Aphid ARP1_G3212 2 36 1 1 ubiquitin E3 ubiquitin-protein ligase mib1 ARP1_G2652 2 93 0.9 1 ubiquitin proteasome subunit beta type 5,8 ARP1_G1052 2 80 1.2 2 ubiquitin hect E3 ubiquitin ligase ARP1_G2247 3 98/99 1 1 Ubiquitin Ufm1-conjugating enzyme 1 ARP1_G4703 2 87 1 1 Ubiquitin ubiquitin carboxyl-terminal hydrolase 38 ARP1_G4246 2 77 1 1 UBX domain-containing protein 8 UBX domain-containing protein 8 ARP1_G3102 2 39 0.9 1 UPF0389 protein growth and transformation-dependent protein ARP1_G12812 3* 40/45 0 0 UPF0462 protein C4orf33 homolog

don gilbert Page 7 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G1034 2 76 1.1 2 voltage-gated chloride channel chloride channel protein 3 ARP1_G578 3 76/78 1.5 2 voltage-gated potassium channel voltage-gated potassium channel ARP1_G4627 2 25 1 2 voltage-gated sodium channel tipe protein (temperature-induced paralytic e) ARP1_G2024 3 84/96 1 1 WD40_repeat WD repeat protein 3 ARP1_G2935 2 99 1 1 WD40_repeat WD repeat protein 61 ARP1_G1321 2 72 1 1 WD40_repeat wd-repeat protein ARP1_G9202 6* 80/91 0 1 WD40_repeat ARP1_G3889 2 96 0.9 1 WD40_repeat receptor for activated C kinase ARP1_G1734 3 97/97 1 1 WH2 Wiskott-Aldrich syndrome protein (WASp) ARP1_G2358 2 na 1 1 WW conserved hypothetical protein ARP1_G10083 5* 57/100 0 0 XK-related protein 7 ARP1_G234 10* 73/99 2 5 Y_phosphatase Protein tyrosine phosphatase 99A CG11516-PB ARP1_G1713 2 55 1 1 zinc ion binding conserved hypothetical protein ARP1_G1939 2 88 1 1 zinc ion binding conserved hypothetical protein ARP1_G2044 14* 55/98 0 1 zinc ion binding ARP1_G2274 2 83 1.1 2 zinc superoxide dismutase Cu-Zn superoxide dismutase 1 ARP1_G400 4 59/90 1.7 3 Zinc/iron transporter Zinc/iron regulated transporter-related protein 3 CG6898-PA ^Aphid ARP1_G4161 2 70 0.8 1 Znfinger zinc finger protein ARP1_G4167 2 98 1 1 Znfinger Zinc finger protein 183. ARP1_G1220 6* 39/65 1 6 Znfinger NFX1-type zinc finger-containing protein 1 ^Aphid ARP1_G7532 4* 41/51 0.4 5 Znfinger ^Aphid ARP1_G7028 5* 91/100 0.4 2 Znfinger RanBP-type and C3HC4-type zinc finger-containing protein 1 # # Unclassified # ARP1_G10034 5* 43/49 0 1 none ARP1_G10054 5* 88/100 0 0 none ARP1_G10070 5* 57/100 0 1 none ARP1_G10084 3* 55/99 0.2 3 none ARP1_G10092 4* 84/100 0.1 1 none ARP1_G10591 4* 80/99 0 1 none ARP1_G1092 2 63 1 1 none coro CG9446-PC ARP1_G11092 4* 52/91 0 1 none ARP1_G11155 3* 51/54 0 0 none ARP1_G11165 4* 71/97 0 0 none ARP1_G11167 4* 32/35 0 1 none ARP1_G11178 4* 64/83 0 1 none ARP1_G1144 10* 52/94 0.7 2 none conserved hypothetical protein ARP1_G1155 15* 70/100 0.2 3 none ARP1_G11711 3* 80/90 0 1 none ARP1_G11773 3* 59/70 0 1 none Protein Star ARP1_G1215 16* 36/46 0 1 none ARP1_G12478 3* 55/82 0 1 none ARP1_G12690 3* 45/59 0 1 none ARP1_G12753 3* 88/98 0 1 none conserved hypothetical protein ARP1_G12795 3* 56/90 0 1 none ARP1_G12968 3* 90/100 0 0 none ARP1_G1355 3 75/76 1.1 2 none triosephosphate isomerase ARP1_G1370 16* 46/79 0 1 none ARP1_G1415 4 41/54 1.1 2 none conserved hypothetical protein ARP1_G1527 2 100 1 1 none ARP1_G1807 6 55/100 1 2 none endoU protein ARP1_G2111 2 40 1.1 2 none conserved hypothetical protein ARP1_G216 40* 52/100 0 1 none ARP1_G2221 14* 45/96 0 1 none ARP1_G2322 4 91/100 0.9 1 none conserved hypothetical protein ARP1_G2365 2 98 1.1 2 none 14 days pregnant adult female placenta cDNA ARP1_G2428 2 41 1.1 2 none conserved hypothetical protein ARP1_G2435 14* 34/95 0 1 none ARP1_G2646 2 91 1 1 none conserved hypothetical protein ARP1_G283 33* 40/94 0 1 none CG33145 CG33145-PA ARP1_G3265 2 100 1.1 2 none COP9 complex homolog subunit 1 b CG3889-PA ARP1_G3336 4 76/98 1 1 none conserved hypothetical protein ARP1_G3679 3 83/92 0.8 1 none conserved hypothetical protein ARP1_G3894 2 81 1 1 none 1110059p08rik-like protein ARP1_G4189 6* 52/100 0.6 5 none ARP1_G4688 2 55 0.8 1 none conserved hypothetical protein ARP1_G4740 12* 44/73 0 1 none salivary C-type lectin ARP1_G4746 11* 56/99 0 0 none ARP1_G4983 2 99 0.9 2 none conserved hypothetical protein ARP1_G5392 2 50 0.9 1 none conserved hypothetical protein

don gilbert Page 8 3/27/09 Daphnia orthology gene groups with over-abundances compared to insects Gene Group Daph dPI iAve iMax Gene_Class Description ARP1_G5650 2 91 0.8 1 none short gastrulation CG9224-PA ARP1_G5697 2 na 0.9 1 none conserved hypothetical protein ARP1_G6324 10* 42/55 0 1 none ARP1_G6460 2 100 0.8 1 none Nasonia vitripennis hypothetical protein ARP1_G660 10* 49/82 1 12 none ARP1_G6758 2 29 0.8 1 none CG6321 CG6321-PA ARP1_G6921 4* 85/99 0.5 4 none ^Aphid ARP1_G6965 5* 52/81 0 1 none ARP1_G699 7 97/100 1.2 3 none conserved hypothetical protein ARP1_G706 16* 41/90 0.4 3 none CG33182 CG33182-PA ARP1_G7536 8* 60/99 0 1 none ARP1_G7559 8* 94/98 0 1 none ARP1_G7615 4* 67/100 0.1 2 none ARP1_G7649 8* 43/95 0 1 none ARP1_G7915 8* 52/100 0 1 none conserved hypothetical protein ARP1_G7922 8* 52/97 0 1 none ARP1_G8131 4* 96/98 0.3 3 none Nasonia vitripennis hypothetical protein ARP1_G8165 7* 29/36 0 1 none ARP1_G8478 7* 40/56 0 1 none ARP1_G8485 7* 42/77 0 1 none ARP1_G8659 5* 49/76 0.1 2 none ARP1_G89 29* 66/100 3.4 22 none Nasonia vitripennis hypothetical protein ARP1_G905 2 28 1 1 none conserved hypothetical protein ARP1_G9119 6* 70/99 0 1 none ARP1_G9122 3* 69/94 0.3 3 none Putative uncharacterized protein ARP1_G9146 5* 42/77 0.1 2 none antifreeze protein ARP1_G9158 6* 60/98 0 0 none ARP1_G942 16* 49/91 0.2 2 none ARP1_G9489 5* 32/48 0 1 none ARP1_G97 62* 75/100 0.1 2 none ARP1_G975 18* 51/96 0 1 none ARP1_G2936 2 98 1 1 none conserved hypothetical protein ARP1_G1710 3 65/67 1 1 none conserved hypothetical protein ARP1_G3874 2 100 1 1 none conserved hypothetical protein ARP1_G3687 2 97 1 1 none conserved hypothetical protein ARP1_G3150 11* 71/98 0.2 2 none ARP1_G1007 2 57 1 2 none beta-hexosaminidase beta chain ARP1_G6649 2 48 0.8 2 none conserved hypothetical protein ARP1_G6706 3 68/89 0.8 2 none conserved hypothetical protein ARP1_G6778 2 53 0.8 2 none M-spondin CG10145-PA ARP1_G7772 2 60 0.7 1 none ADP-ribosylation factor-like 6 interacting protein 4 ARP1_G3427 2 93 1 1 none conserved hypothetical protein ARP1_G2926 4 48/68 0.8 1 none conserved hypothetical protein ARP1_G10056 4* 67/98 0 1 none ARP1_G4195 2 90 0.9 1 none conserved hypothetical protein ARP1_G4488 3 61/70 0.7 1 none conserved hypothetical protein # # Transposons # ARP1_G120 47* 44/100 1 11 TE/DNA integration ARP1_G106 59* 46/100 0.1 1 TE/RNA-directed DNA polymerase ARP1_G318 27* 51/99 0.4 4 TE?/GfV-C17-ORF1 ^Aphid ARP1_G2021 12* 61/96 0.2 2 TE polyprotein # # SUM Transposons: Over=145, PI=50, PM=99, iAve=2, iMax=18, ngroup=4 # # SUM Unclassified: Over=647, PI=65, PM=83, iAve=50, iMax=166, ngroup=100 # # SUM Regular: Over=2621, PI=74, PM=82, iAve=610, iMax=1017, ngroup=589 #

don gilbert Page 9 3/27/09